YNL098C / RAS2

GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL098C / RAS2

Click on Significant Values for Screen Details ID:SGTC_18|Compound:0111-0177|FD-Score:5.22|P-value:2.34E-6 ID:SGTC_22|Compound:0964-0034|FD-Score:4.89|P-value:9.24E-6 ID:SGTC_150|Compound:3970-0969|FD-Score:6.95|P-value:4.54E-10 ID:SGTC_168|Compound:3970-0862|FD-Score:5.66|P-value:3.32E-7 ID:SGTC_173|Compound:1273-0061|FD-Score:3.62|P-value:8.25E-4 ID:SGTC_210|Compound:3551-0445|FD-Score:3.67|P-value:7.03E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:4.03|P-value:2.24E-4 ID:SGTC_214|Compound:4373-3496|FD-Score:3.72|P-value:6.03E-4 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:4.53|P-value:3.77E-5 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:5.66|P-value:3.38E-7 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:7.24|P-value:8.96E-11 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:3.87|P-value:3.76E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:6.08|P-value:4.49E-8 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:5.71|P-value:2.60E-7 ID:SGTC_319|Compound:1348-1596|FD-Score:3.69|P-value:6.73E-4 ID:SGTC_597|Compound:k018-0003|FD-Score:-3.39|P-value:9.29E-4 ID:SGTC_611|Compound:3453-1652|FD-Score:3.66|P-value:7.37E-4 ID:SGTC_815|Compound:1582-0027|FD-Score:4.81|P-value:1.27E-5 ID:SGTC_944|Compound:3370-0224|FD-Score:-3.83|P-value:2.27E-4 ID:SGTC_960|Compound:1222-0186|FD-Score:3.82|P-value:4.46E-4 ID:SGTC_992|Compound:3909-8734|FD-Score:5.05|P-value:4.76E-6 ID:SGTC_993|Compound:3909-8757|FD-Score:-3.52|P-value:6.11E-4 ID:SGTC_1151|Compound:3909-8316|FD-Score:3.94|P-value:3.03E-4 ID:SGTC_1323|Compound:1348-1351|FD-Score:3.87|P-value:3.73E-4 ID:SGTC_1673|Compound:st016611|FD-Score:-3.57|P-value:5.30E-4 ID:SGTC_1682|Compound:st012968|FD-Score:-6.75|P-value:4.67E-10 ID:SGTC_1817|Compound:st052116|FD-Score:3.97|P-value:2.72E-4 ID:SGTC_1963|Compound:st076565|FD-Score:3.88|P-value:3.65E-4 ID:SGTC_1974|Compound:st069448|FD-Score:4.86|P-value:1.04E-5 ID:SGTC_2020|Compound:4017018|FD-Score:3.80|P-value:4.72E-4 ID:SGTC_2038|Compound:4100038|FD-Score:4.29|P-value:8.91E-5 ID:SGTC_2039|Compound:Aminomethylbenzodioxan|FD-Score:-4.11|P-value:8.53E-5 ID:SGTC_2273|Compound:7815444|FD-Score:3.68|P-value:6.87E-4 ID:SGTC_2440|Compound:5628896|FD-Score:3.88|P-value:3.66E-4 ID:SGTC_2481|Compound:5478948|FD-Score:5.20|P-value:2.60E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:9.89|P-value:1.10E-18 ID:SGTC_2610|Compound:ononetin|FD-Score:3.94|P-value:2.99E-4 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:-3.67|P-value:3.80E-4 ID:SGTC_2870|Compound:9038528|FD-Score:4.51|P-value:4.02E-5 ID:SGTC_2906|Compound:7926292|FD-Score:4.99|P-value:6.30E-6 ID:SGTC_2962|Compound:9082311|FD-Score:-3.57|P-value:5.32E-4 ID:SGTC_2963|Compound:9082507|FD-Score:4.86|P-value:1.05E-5 ID:SGTC_18|Compound:0111-0177|FD-Score:5.22|P-value:2.34E-6 ID:SGTC_22|Compound:0964-0034|FD-Score:4.89|P-value:9.24E-6 ID:SGTC_150|Compound:3970-0969|FD-Score:6.95|P-value:4.54E-10 ID:SGTC_168|Compound:3970-0862|FD-Score:5.66|P-value:3.32E-7 ID:SGTC_173|Compound:1273-0061|FD-Score:3.62|P-value:8.25E-4 ID:SGTC_210|Compound:3551-0445|FD-Score:3.67|P-value:7.03E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:4.03|P-value:2.24E-4 ID:SGTC_214|Compound:4373-3496|FD-Score:3.72|P-value:6.03E-4 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:4.53|P-value:3.77E-5 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:5.66|P-value:3.38E-7 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:7.24|P-value:8.96E-11 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:3.87|P-value:3.76E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:6.08|P-value:4.49E-8 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:5.71|P-value:2.60E-7 ID:SGTC_319|Compound:1348-1596|FD-Score:3.69|P-value:6.73E-4 ID:SGTC_597|Compound:k018-0003|FD-Score:-3.39|P-value:9.29E-4 ID:SGTC_611|Compound:3453-1652|FD-Score:3.66|P-value:7.37E-4 ID:SGTC_815|Compound:1582-0027|FD-Score:4.81|P-value:1.27E-5 ID:SGTC_944|Compound:3370-0224|FD-Score:-3.83|P-value:2.27E-4 ID:SGTC_960|Compound:1222-0186|FD-Score:3.82|P-value:4.46E-4 ID:SGTC_992|Compound:3909-8734|FD-Score:5.05|P-value:4.76E-6 ID:SGTC_993|Compound:3909-8757|FD-Score:-3.52|P-value:6.11E-4 ID:SGTC_1151|Compound:3909-8316|FD-Score:3.94|P-value:3.03E-4 ID:SGTC_1323|Compound:1348-1351|FD-Score:3.87|P-value:3.73E-4 ID:SGTC_1673|Compound:st016611|FD-Score:-3.57|P-value:5.30E-4 ID:SGTC_1682|Compound:st012968|FD-Score:-6.75|P-value:4.67E-10 ID:SGTC_1817|Compound:st052116|FD-Score:3.97|P-value:2.72E-4 ID:SGTC_1963|Compound:st076565|FD-Score:3.88|P-value:3.65E-4 ID:SGTC_1974|Compound:st069448|FD-Score:4.86|P-value:1.04E-5 ID:SGTC_2020|Compound:4017018|FD-Score:3.80|P-value:4.72E-4 ID:SGTC_2038|Compound:4100038|FD-Score:4.29|P-value:8.91E-5 ID:SGTC_2039|Compound:Aminomethylbenzodioxan|FD-Score:-4.11|P-value:8.53E-5 ID:SGTC_2273|Compound:7815444|FD-Score:3.68|P-value:6.87E-4 ID:SGTC_2440|Compound:5628896|FD-Score:3.88|P-value:3.66E-4 ID:SGTC_2481|Compound:5478948|FD-Score:5.20|P-value:2.60E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:9.89|P-value:1.10E-18 ID:SGTC_2610|Compound:ononetin|FD-Score:3.94|P-value:2.99E-4 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:-3.67|P-value:3.80E-4 ID:SGTC_2870|Compound:9038528|FD-Score:4.51|P-value:4.02E-5 ID:SGTC_2906|Compound:7926292|FD-Score:4.99|P-value:6.30E-6 ID:SGTC_2962|Compound:9082311|FD-Score:-3.57|P-value:5.32E-4 ID:SGTC_2963|Compound:9082507|FD-Score:4.86|P-value:1.05E-5

Top fitness defect scores for YNL098C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2582 agaric acid 9.89 100.0 µM 1.10E-18 redox potentiating 64 2.37E-23
2 SGTC_240 5hr heat shock (37°C) + Streptovitacin 7.24 300.0 µM 8.96E-11 heat shock/prefoldin 11 2.31E-13
3 SGTC_150 3970-0969 6.95 77.2 µM 4.54E-10 13 1.79E-12
4 SGTC_242 5hr heat shock (37°C) + 5-fluorouracil 6.08 4.6 µM 4.49E-8 heat shock/prefoldin 14 5.96E-10
5 SGTC_243 5hr heat shock (37°C) + splitomicin 5.71 1.0 µM 2.60E-7 heat shock/prefoldin 18 5.49E-9
6 SGTC_168 3970-0862 5.66 530.5 µM 3.32E-7 52 7.48E-9
7 SGTC_239 5hr heat shock (37°C) + mitomycin C 5.66 819.4 µM 3.38E-7 heat shock/prefoldin 13 7.65E-9
8 SGTC_18 0111-0177 5.22 500.0 nM 2.34E-6 29 8.85E-8
9 SGTC_2481 5478948 5.20 45.2 µM 2.60E-6 PDR1 14 1.01E-7
10 SGTC_992 3909-8734 5.05 162.0 µM 4.76E-6 5 2.17E-7
11 SGTC_2906 7926292 4.99 26.4 µM 6.30E-6 10 3.10E-7
12 SGTC_22 0964-0034 4.89 14.5 µM 9.24E-6 unfolded protein response 29 5.03E-7
13 SGTC_1974 st069448 4.86 26.9 µM 1.04E-5 11 5.86E-7
14 SGTC_2963 9082507 4.86 9.0 µM 1.05E-5 11 5.89E-7
15 SGTC_815 1582-0027 4.81 36.9 µM 1.27E-5 8 7.53E-7
16 SGTC_238 5hr heat shock (37°C) + nsc-638432 4.53 8.9 µM 3.77E-5 heat shock/prefoldin 26 2.98E-6
17 SGTC_2870 9038528 4.51 13.0 µM 4.02E-5 13 3.23E-6
18 SGTC_2038 4100038 4.29 31.0 µM 8.91E-5 plasma membrane duress 38 8.84E-6
19 SGTC_212 3807-4682 4.03 38.8 µM 2.24E-4 tubulin folding & SWR complex 66 2.83E-5
20 SGTC_1817 st052116 3.97 64.4 µM 2.72E-4 RNA processing & uracil transport 12 3.63E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.327 3.17E-84 YLR027C AAT2 hom Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells
0.271 1.62E-57 YDR260C SWM1 hom Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation
0.270 4.59E-57 YDR069C DOA4 hom Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole
0.253 5.08E-50 YLR200W YKE2 hom Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin
0.242 6.79E-46 YCR063W BUD31 hom Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis
0.195 3.35E-30 YDR457W TOM1 hom E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase
0.182 1.66E-26 YML103C NUP188 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188
0.177 3.66E-25 YHR041C SRB2 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance
0.176 9.92E-25 YNL106C INP52 hom Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain
0.172 8.79E-24 YOR001W RRP6 hom Nuclear exosome exonuclease component; has 3'-5' exonuclease activity; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay
0.167 1.71E-22 YKL007W CAP1 hom Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress
0.160 9.65E-21 YPL232W SSO1 hom Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p
0.159 1.56E-20 YIL129C TAO3 het Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p
0.156 1.09E-19 YMR109W MYO5 hom One of two type I myosins; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization
0.154 2.55E-19 YOR049C RSB1 hom Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane