YNL102W / POL1

Catalytic subunit of the DNA polymerase I alpha-primase complex, required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis

Zygosity: Heterozygous strain
fixedexpanded
Profile for YNL102W / POL1

Click on Significant Values for Screen Details ID:SGTC_116|Compound:0155-0182|FD-Score:-4.96|P-value:1.06E-7 ID:SGTC_141|Compound:0443-0274|FD-Score:-3.88|P-value:2.41E-5 ID:SGTC_145|Compound:3737-0113|FD-Score:3.91|P-value:2.83E-5 ID:SGTC_146|Compound:3448-8650|FD-Score:-3.70|P-value:5.32E-5 ID:SGTC_151|Compound:3013-0144|FD-Score:5.12|P-value:6.41E-8 ID:SGTC_414|Compound:piperonyl butoxide|FD-Score:3.04|P-value:9.20E-4 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:-3.05|P-value:6.81E-4 ID:SGTC_527|Compound:4476-4215|FD-Score:3.72|P-value:6.62E-5 ID:SGTC_921|Compound:1561-0023|FD-Score:3.12|P-value:6.91E-4 ID:SGTC_942|Compound:3253-1115|FD-Score:7.52|P-value:3.92E-15 ID:SGTC_949|Compound:1165-0505|FD-Score:4.11|P-value:1.19E-5 ID:SGTC_1039|Compound:k821-0043|FD-Score:3.36|P-value:2.83E-4 ID:SGTC_1076|Compound:itraconazole|FD-Score:3.12|P-value:6.80E-4 ID:SGTC_1101|Compound:ebselen|FD-Score:3.25|P-value:4.25E-4 ID:SGTC_1122|Compound:capsaicin|FD-Score:-3.03|P-value:7.30E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-3.16|P-value:4.49E-4 ID:SGTC_1198|Compound:1612-1005|FD-Score:-3.45|P-value:1.47E-4 ID:SGTC_1582|Compound:3',3',6'-trihydroxyflavone|FD-Score:-3.20|P-value:3.84E-4 ID:SGTC_1886|Compound:st057356|FD-Score:3.23|P-value:4.49E-4 ID:SGTC_2000|Compound:st075254|FD-Score:3.78|P-value:4.99E-5 ID:SGTC_2003|Compound:4023581|FD-Score:3.87|P-value:3.51E-5 ID:SGTC_2010|Compound:4012422|FD-Score:4.04|P-value:1.62E-5 ID:SGTC_2054|Compound:5233173|FD-Score:-4.58|P-value:8.47E-7 ID:SGTC_2199|Compound:6590217|FD-Score:3.31|P-value:3.32E-4 ID:SGTC_2287|Compound:7945887|FD-Score:-3.84|P-value:2.84E-5 ID:SGTC_2346|Compound:9031566|FD-Score:3.13|P-value:6.62E-4 ID:SGTC_2377|Compound:9073351|FD-Score:4.67|P-value:7.58E-7 ID:SGTC_2385|Compound:9074692|FD-Score:3.97|P-value:2.26E-5 ID:SGTC_2450|Compound:5739737|FD-Score:3.14|P-value:6.39E-4 ID:SGTC_2515|Compound:chrysin|FD-Score:3.01|P-value:9.94E-4 ID:SGTC_2525|Compound:harmine|FD-Score:3.41|P-value:2.29E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:4.44|P-value:2.41E-6 ID:SGTC_2638|Compound:epoxomicin|FD-Score:3.15|P-value:6.05E-4 ID:SGTC_2853|Compound:9015042|FD-Score:5.25|P-value:3.15E-8 ID:SGTC_2856|Compound:9022925|FD-Score:-5.41|P-value:7.91E-9 ID:SGTC_2892|Compound:9047358|FD-Score:-3.14|P-value:4.98E-4 ID:SGTC_3211|Compound:9128781|FD-Score:-3.32|P-value:2.45E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:-4.96|P-value:1.06E-7 ID:SGTC_141|Compound:0443-0274|FD-Score:-3.88|P-value:2.41E-5 ID:SGTC_145|Compound:3737-0113|FD-Score:3.91|P-value:2.83E-5 ID:SGTC_146|Compound:3448-8650|FD-Score:-3.70|P-value:5.32E-5 ID:SGTC_151|Compound:3013-0144|FD-Score:5.12|P-value:6.41E-8 ID:SGTC_414|Compound:piperonyl butoxide|FD-Score:3.04|P-value:9.20E-4 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:-3.05|P-value:6.81E-4 ID:SGTC_527|Compound:4476-4215|FD-Score:3.72|P-value:6.62E-5 ID:SGTC_921|Compound:1561-0023|FD-Score:3.12|P-value:6.91E-4 ID:SGTC_942|Compound:3253-1115|FD-Score:7.52|P-value:3.92E-15 ID:SGTC_949|Compound:1165-0505|FD-Score:4.11|P-value:1.19E-5 ID:SGTC_1039|Compound:k821-0043|FD-Score:3.36|P-value:2.83E-4 ID:SGTC_1076|Compound:itraconazole|FD-Score:3.12|P-value:6.80E-4 ID:SGTC_1101|Compound:ebselen|FD-Score:3.25|P-value:4.25E-4 ID:SGTC_1122|Compound:capsaicin|FD-Score:-3.03|P-value:7.30E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-3.16|P-value:4.49E-4 ID:SGTC_1198|Compound:1612-1005|FD-Score:-3.45|P-value:1.47E-4 ID:SGTC_1582|Compound:3',3',6'-trihydroxyflavone|FD-Score:-3.20|P-value:3.84E-4 ID:SGTC_1886|Compound:st057356|FD-Score:3.23|P-value:4.49E-4 ID:SGTC_2000|Compound:st075254|FD-Score:3.78|P-value:4.99E-5 ID:SGTC_2003|Compound:4023581|FD-Score:3.87|P-value:3.51E-5 ID:SGTC_2010|Compound:4012422|FD-Score:4.04|P-value:1.62E-5 ID:SGTC_2054|Compound:5233173|FD-Score:-4.58|P-value:8.47E-7 ID:SGTC_2199|Compound:6590217|FD-Score:3.31|P-value:3.32E-4 ID:SGTC_2287|Compound:7945887|FD-Score:-3.84|P-value:2.84E-5 ID:SGTC_2346|Compound:9031566|FD-Score:3.13|P-value:6.62E-4 ID:SGTC_2377|Compound:9073351|FD-Score:4.67|P-value:7.58E-7 ID:SGTC_2385|Compound:9074692|FD-Score:3.97|P-value:2.26E-5 ID:SGTC_2450|Compound:5739737|FD-Score:3.14|P-value:6.39E-4 ID:SGTC_2515|Compound:chrysin|FD-Score:3.01|P-value:9.94E-4 ID:SGTC_2525|Compound:harmine|FD-Score:3.41|P-value:2.29E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:4.44|P-value:2.41E-6 ID:SGTC_2638|Compound:epoxomicin|FD-Score:3.15|P-value:6.05E-4 ID:SGTC_2853|Compound:9015042|FD-Score:5.25|P-value:3.15E-8 ID:SGTC_2856|Compound:9022925|FD-Score:-5.41|P-value:7.91E-9 ID:SGTC_2892|Compound:9047358|FD-Score:-3.14|P-value:4.98E-4 ID:SGTC_3211|Compound:9128781|FD-Score:-3.32|P-value:2.45E-4

Top fitness defect scores for YNL102W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_942 3253-1115 7.52 227.0 µM 3.92E-15 2 2.72E-14 2.31
2 SGTC_2853 9015042 5.25 58.4 µM 3.15E-8 2 7.70E-8 0.96
3 SGTC_151 3013-0144 5.12 2.3 µM 6.41E-8 iron homeostasis 5 1.50E-7 0.93
4 SGTC_2377 9073351 4.67 62.6 µM 7.58E-7 ergosterol biosynthesis 4 1.52E-6 0.07
5 SGTC_2566 crystal violet carbinol 4.44 226.0 nM 2.41E-6 10 4.49E-6 0.04
6 SGTC_949 1165-0505 4.11 146.0 µM 1.19E-5 6 2.01E-5 0.14
7 SGTC_2010 4012422 4.04 159.0 µM 1.62E-5 6 2.68E-5 0.39
8 SGTC_2385 9074692 3.97 200.0 µM 2.26E-5 5 3.66E-5 0.23
9 SGTC_145 3737-0113 3.91 38.4 µM 2.83E-5 DNA intercalators 51 4.53E-5 0.03
10 SGTC_2003 4023581 3.87 200.0 µM 3.51E-5 cell wall signaling 9 5.54E-5 0.20
11 SGTC_2000 st075254 3.78 36.4 µM 4.99E-5 4 7.72E-5 0.07
12 SGTC_527 4476-4215 3.72 268.0 µM 6.62E-5 PDR1 4 1.01E-4 0.04
13 SGTC_2525 harmine 3.41 47.9 µM 2.29E-4 ergosterol biosynthesis 6 3.23E-4 0.02
14 SGTC_1039 k821-0043 3.36 94.0 µM 2.83E-4 Golgi 11 3.94E-4 0.20
15 SGTC_2199 6590217 3.31 200.0 µM 3.32E-4 mitochondrial processes 6 4.59E-4 0.05
16 SGTC_1101 ebselen 3.25 5.3 µM 4.25E-4 11 5.78E-4 0.14
17 SGTC_1886 st057356 3.23 49.9 µM 4.49E-4 60S ribosome export 15 6.09E-4 0.11
18 SGTC_2638 epoxomicin 3.15 18.0 µM 6.05E-4 7 8.06E-4 0.15
19 SGTC_2450 5739737 3.14 12.9 µM 6.39E-4 10 8.50E-4 0.12
20 SGTC_2346 9031566 3.13 143.8 µM 6.62E-4 Golgi 18 8.77E-4 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.138 1.23E-15 YLR392C ART10 hom Protein of unknown function that contains 2 PY motifs and is ubiquinated by Rsp5p; overexpression confers resistance to arsenite; green fluorescent protein (GFP)-fusion protein localizes it to the cytoplasm; non-essential gene
0.132 1.67E-14 YPL123C RNY1 hom Vacuolar RNase of the T(2) family, relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; promotes apoptosis under stress conditions and this function is independent of its catalytic activity
0.126 2.74E-13 YOR159C SME1 het Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
0.123 7.29E-13 YDR054C CDC34 het Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress
0.118 5.96E-12 YAR008W SEN34 het Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease
0.113 5.04E-11 YDR478W SNM1 het Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP
0.111 1.33E-10 YBL004W UTP20 het Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA
0.108 4.16E-10 YER171W RAD3 het 5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress
0.107 5.81E-10 YOR304W ISW2 hom ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth
0.105 8.89E-10 YJL143W TIM17 het Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex
0.103 2.31E-9 YDR013W PSF1 het Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
0.101 4.17E-9 YDR201W SPC19 het Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body
0.100 6.63E-9 YNL163C RIA1 het Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis; with Sdo1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; required for quality control check of newly made large ribosomal subunits before they are released into the pool of translating ribosomes
0.099 9.50E-9 YNL137C NAM9 het Mitochondrial ribosomal component of the small subunit
0.096 2.23E-8 YER013W PRP22 het DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes