YNL103W / MET4

Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p

Zygosity: Heterozygous strain
fixedexpanded
Profile for YNL103W / MET4

Click on Significant Values for Screen Details ID:SGTC_23|Compound:0213-0011|FD-Score:3.57|P-value:3.63E-4 ID:SGTC_34|Compound:0469-0683|FD-Score:4.51|P-value:9.57E-6 ID:SGTC_44|Compound:0929-0055|FD-Score:-10.80|P-value:2.59E-25 ID:SGTC_67|Compound:0335-0847|FD-Score:4.40|P-value:1.53E-5 ID:SGTC_72|Compound:0744-0168|FD-Score:-3.79|P-value:1.49E-4 ID:SGTC_115|Compound:0527-0192|FD-Score:-5.81|P-value:1.48E-8 ID:SGTC_159|Compound:3970-0959|FD-Score:3.69|P-value:2.33E-4 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:3.41|P-value:6.22E-4 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:3.61|P-value:3.14E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:4.39|P-value:1.58E-5 ID:SGTC_690|Compound:glyoxal|FD-Score:9.04|P-value:4.58E-18 ID:SGTC_758|Compound:0868-0029|FD-Score:-10.50|P-value:1.15E-23 ID:SGTC_805|Compound:3852-0218|FD-Score:-3.43|P-value:5.33E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:4.84|P-value:2.17E-6 ID:SGTC_914|Compound:3820-6779|FD-Score:-3.49|P-value:4.33E-4 ID:SGTC_968|Compound:1309-0633|FD-Score:4.33|P-value:1.97E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:-4.31|P-value:1.94E-5 ID:SGTC_1333|Compound:1482-0047|FD-Score:3.81|P-value:1.51E-4 ID:SGTC_1420|Compound:4'-methoxyflavone|FD-Score:3.45|P-value:5.39E-4 ID:SGTC_1491|Compound:4138-0063|FD-Score:9.48|P-value:1.07E-19 ID:SGTC_1500|Compound:4488-1866|FD-Score:3.38|P-value:6.80E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:3.42|P-value:5.93E-4 ID:SGTC_1751|Compound:st044839|FD-Score:4.04|P-value:6.41E-5 ID:SGTC_1829|Compound:berberine|FD-Score:4.14|P-value:4.32E-5 ID:SGTC_2077|Compound:5212015|FD-Score:4.71|P-value:4.01E-6 ID:SGTC_2117|Compound:5521686|FD-Score:-3.63|P-value:2.62E-4 ID:SGTC_2239|Compound:6685329|FD-Score:3.57|P-value:3.56E-4 ID:SGTC_2330|Compound:7979373|FD-Score:-4.27|P-value:2.33E-5 ID:SGTC_2409|Compound:alverine citrate|FD-Score:5.77|P-value:2.18E-8 ID:SGTC_2423|Compound:st077119|FD-Score:-4.11|P-value:4.44E-5 ID:SGTC_2436|Compound:5633522|FD-Score:-3.96|P-value:7.93E-5 ID:SGTC_2605|Compound:digitonin|FD-Score:8.93|P-value:1.14E-17 ID:SGTC_2607|Compound:solasodine|FD-Score:3.56|P-value:3.72E-4 ID:SGTC_2616|Compound:abietic acid|FD-Score:6.03|P-value:5.26E-9 ID:SGTC_2629|Compound:bisabolol|FD-Score:3.76|P-value:1.84E-4 ID:SGTC_2700|Compound:st077718|FD-Score:-3.66|P-value:2.34E-4 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:3.59|P-value:3.32E-4 ID:SGTC_2852|Compound:9023854|FD-Score:3.98|P-value:7.97E-5 ID:SGTC_2906|Compound:7926292|FD-Score:-3.40|P-value:5.79E-4 ID:SGTC_2973|Compound:9094665|FD-Score:3.38|P-value:6.89E-4 ID:SGTC_3005|Compound:9076496|FD-Score:-3.32|P-value:7.64E-4 ID:SGTC_3012|Compound:9080433|FD-Score:3.73|P-value:2.02E-4 ID:SGTC_3016|Compound:9080866|FD-Score:-3.44|P-value:5.03E-4 ID:SGTC_3217|Compound:9129673|FD-Score:4.81|P-value:2.54E-6 ID:SGTC_3323|Compound:9138564|FD-Score:11.60|P-value:8.79E-29 ID:SGTC_3328|Compound:9140624|FD-Score:10.30|P-value:4.04E-23 ID:SGTC_3329|Compound:9141233|FD-Score:12.10|P-value:7.84E-31 ID:SGTC_3331|Compound:9141060|FD-Score:6.47|P-value:4.08E-10 ID:SGTC_3335|Compound:9143315|FD-Score:-4.21|P-value:2.94E-5 ID:SGTC_23|Compound:0213-0011|FD-Score:3.57|P-value:3.63E-4 ID:SGTC_34|Compound:0469-0683|FD-Score:4.51|P-value:9.57E-6 ID:SGTC_44|Compound:0929-0055|FD-Score:-10.80|P-value:2.59E-25 ID:SGTC_67|Compound:0335-0847|FD-Score:4.40|P-value:1.53E-5 ID:SGTC_72|Compound:0744-0168|FD-Score:-3.79|P-value:1.49E-4 ID:SGTC_115|Compound:0527-0192|FD-Score:-5.81|P-value:1.48E-8 ID:SGTC_159|Compound:3970-0959|FD-Score:3.69|P-value:2.33E-4 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:3.41|P-value:6.22E-4 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:3.61|P-value:3.14E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:4.39|P-value:1.58E-5 ID:SGTC_690|Compound:glyoxal|FD-Score:9.04|P-value:4.58E-18 ID:SGTC_758|Compound:0868-0029|FD-Score:-10.50|P-value:1.15E-23 ID:SGTC_805|Compound:3852-0218|FD-Score:-3.43|P-value:5.33E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:4.84|P-value:2.17E-6 ID:SGTC_914|Compound:3820-6779|FD-Score:-3.49|P-value:4.33E-4 ID:SGTC_968|Compound:1309-0633|FD-Score:4.33|P-value:1.97E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:-4.31|P-value:1.94E-5 ID:SGTC_1333|Compound:1482-0047|FD-Score:3.81|P-value:1.51E-4 ID:SGTC_1420|Compound:4'-methoxyflavone|FD-Score:3.45|P-value:5.39E-4 ID:SGTC_1491|Compound:4138-0063|FD-Score:9.48|P-value:1.07E-19 ID:SGTC_1500|Compound:4488-1866|FD-Score:3.38|P-value:6.80E-4 ID:SGTC_1511|Compound:0906-3109|FD-Score:3.42|P-value:5.93E-4 ID:SGTC_1751|Compound:st044839|FD-Score:4.04|P-value:6.41E-5 ID:SGTC_1829|Compound:berberine|FD-Score:4.14|P-value:4.32E-5 ID:SGTC_2077|Compound:5212015|FD-Score:4.71|P-value:4.01E-6 ID:SGTC_2117|Compound:5521686|FD-Score:-3.63|P-value:2.62E-4 ID:SGTC_2239|Compound:6685329|FD-Score:3.57|P-value:3.56E-4 ID:SGTC_2330|Compound:7979373|FD-Score:-4.27|P-value:2.33E-5 ID:SGTC_2409|Compound:alverine citrate|FD-Score:5.77|P-value:2.18E-8 ID:SGTC_2423|Compound:st077119|FD-Score:-4.11|P-value:4.44E-5 ID:SGTC_2436|Compound:5633522|FD-Score:-3.96|P-value:7.93E-5 ID:SGTC_2605|Compound:digitonin|FD-Score:8.93|P-value:1.14E-17 ID:SGTC_2607|Compound:solasodine|FD-Score:3.56|P-value:3.72E-4 ID:SGTC_2616|Compound:abietic acid|FD-Score:6.03|P-value:5.26E-9 ID:SGTC_2629|Compound:bisabolol|FD-Score:3.76|P-value:1.84E-4 ID:SGTC_2700|Compound:st077718|FD-Score:-3.66|P-value:2.34E-4 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:3.59|P-value:3.32E-4 ID:SGTC_2852|Compound:9023854|FD-Score:3.98|P-value:7.97E-5 ID:SGTC_2906|Compound:7926292|FD-Score:-3.40|P-value:5.79E-4 ID:SGTC_2973|Compound:9094665|FD-Score:3.38|P-value:6.89E-4 ID:SGTC_3005|Compound:9076496|FD-Score:-3.32|P-value:7.64E-4 ID:SGTC_3012|Compound:9080433|FD-Score:3.73|P-value:2.02E-4 ID:SGTC_3016|Compound:9080866|FD-Score:-3.44|P-value:5.03E-4 ID:SGTC_3217|Compound:9129673|FD-Score:4.81|P-value:2.54E-6 ID:SGTC_3323|Compound:9138564|FD-Score:11.60|P-value:8.79E-29 ID:SGTC_3328|Compound:9140624|FD-Score:10.30|P-value:4.04E-23 ID:SGTC_3329|Compound:9141233|FD-Score:12.10|P-value:7.84E-31 ID:SGTC_3331|Compound:9141060|FD-Score:6.47|P-value:4.08E-10 ID:SGTC_3335|Compound:9143315|FD-Score:-4.21|P-value:2.94E-5

Top fitness defect scores for YNL103W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_3329 9141233 12.10 27.6 µM 7.84E-31 1 6.29E-34 7.92
2 SGTC_3323 9138564 11.60 53.9 µM 8.79E-29 1 1.16E-31 5.94
3 SGTC_3328 9140624 10.30 29.0 µM 4.04E-23 1 2.10E-25 5.13
4 SGTC_1491 4138-0063 9.48 26.5 µM 1.07E-19 1 1.27E-21 4.39
5 SGTC_690 glyoxal 9.04 20.3 µM 4.58E-18 4 8.11E-20 0.02
6 SGTC_2605 digitonin 8.93 300.0 nM 1.14E-17 1 2.21E-19 3.00
7 SGTC_3331 9141060 6.47 2.0 µM 4.08E-10 cell wall 2 4.93E-11 0.95
8 SGTC_2616 abietic acid 6.03 100.0 µM 5.26E-9 1 8.31E-10 1.81
9 SGTC_2409 alverine citrate 5.77 64.0 µM 2.18E-8 7 4.00E-9 0.78
10 SGTC_913 3487-1245 4.84 167.0 µM 2.17E-6 7 6.42E-7 0.21
11 SGTC_3217 9129673 4.81 49.5 µM 2.54E-6 5 7.64E-7 0.37
12 SGTC_2077 5212015 4.71 200.0 µM 4.01E-6 6 1.26E-6 0.38
13 SGTC_34 0469-0683 4.51 81.8 µM 9.57E-6 SWF1 & branched chain AA biosynthesis 1 3.29E-6 0.33
14 SGTC_67 0335-0847 4.40 266.0 µM 1.53E-5 ubiquinone biosynthesis & proteasome 15 5.52E-6 0.07
15 SGTC_669 0108-0021 4.39 26.2 µM 1.58E-5 fatty acid desaturase (OLE1) 10 5.73E-6 0.07
16 SGTC_968 1309-0633 4.33 340.0 µM 1.97E-5 2 7.31E-6 0.02
17 SGTC_1829 berberine 4.14 14.4 µM 4.32E-5 60S ribosome export 12 1.74E-5 0.11
18 SGTC_1751 st044839 4.04 27.2 µM 6.41E-5 Golgi 4 2.68E-5 0.36
19 SGTC_2852 9023854 3.98 52.0 µM 7.97E-5 6 3.41E-5 0.29
20 SGTC_1333 1482-0047 3.81 286.0 µM 1.51E-4 7 6.93E-5 0.24

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.254 9.58E-51 YHL045W_d YHL045W_d hom Putative protein of unknown function; not an essential gene
0.227 1.35E-40 YGL090W LIF1 hom Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein
0.219 8.23E-38 YLR040C_p YLR040C_p hom Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential
0.213 1.19E-35 YDL155W CLB3 hom B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress
0.208 3.04E-34 YPL246C RBD2 hom Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p
0.201 7.07E-32 YNL123W NMA111 hom Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases
0.185 3.49E-27 YML029W USA1 hom Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p
0.183 1.17E-26 YDL022W GPD1 hom NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity
0.181 4.44E-26 YOR271C_p FSF1_p hom Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis
0.176 1.22E-24 YGL114W_p YGL114W_p hom Putative protein of unknown function; predicted member of the oligopeptide transporter (OPT) family of membrane transporters
0.167 2.31E-22 YIL009W FAA3 hom Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.155 1.78E-19 YML025C YML6 het Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins
0.154 2.56E-19 YLR317W_d YLR317W_d het Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C
0.143 7.36E-17 YOR033C EXO1 hom 5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress
0.141 2.52E-16 YOR014W RTS1 hom B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A