YNL132W / KRE33

Essential protein, required for biogenesis of the small ribosomal subunit; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance

Zygosity: Heterozygous strain
fixedexpanded
Profile for YNL132W / KRE33

Click on Significant Values for Screen Details ID:SGTC_51|Compound:1013-0126|FD-Score:4.64|P-value:5.31E-7 ID:SGTC_80|Compound:0159-0065|FD-Score:3.47|P-value:1.38E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:-3.92|P-value:1.54E-5 ID:SGTC_90|Compound:0870-0863|FD-Score:-3.39|P-value:1.51E-4 ID:SGTC_96|Compound:0929-0093|FD-Score:-3.55|P-value:7.91E-5 ID:SGTC_258|Compound:haloperidol|FD-Score:3.36|P-value:2.08E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:2.96|P-value:9.50E-4 ID:SGTC_381|Compound:3461-2219|FD-Score:3.57|P-value:9.04E-5 ID:SGTC_418|Compound:lividomycin a|FD-Score:3.25|P-value:3.23E-4 ID:SGTC_493|Compound:zm226600|FD-Score:3.17|P-value:4.42E-4 ID:SGTC_640|Compound:3381-0960|FD-Score:3.22|P-value:3.60E-4 ID:SGTC_644|Compound:1498-1197|FD-Score:3.09|P-value:5.90E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-8.45|P-value:1.69E-19 ID:SGTC_815|Compound:1582-0027|FD-Score:3.59|P-value:8.37E-5 ID:SGTC_817|Compound:1391-0715|FD-Score:4.08|P-value:8.92E-6 ID:SGTC_883|Compound:0850-0245|FD-Score:3.07|P-value:6.35E-4 ID:SGTC_1056|Compound:3448-1962|FD-Score:3.79|P-value:3.40E-5 ID:SGTC_1068|Compound:loratadine|FD-Score:3.02|P-value:7.67E-4 ID:SGTC_1087|Compound:pterostilbene|FD-Score:3.72|P-value:4.67E-5 ID:SGTC_1358|Compound:1509-0022|FD-Score:4.69|P-value:4.02E-7 ID:SGTC_1433|Compound:4060-0023|FD-Score:4.17|P-value:6.04E-6 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.05|P-value:6.96E-4 ID:SGTC_1782|Compound:st039423|FD-Score:3.47|P-value:1.35E-4 ID:SGTC_1969|Compound:st069479|FD-Score:-3.79|P-value:2.80E-5 ID:SGTC_1972|Compound:st070201|FD-Score:-3.07|P-value:5.40E-4 ID:SGTC_1979|Compound:st071809|FD-Score:4.48|P-value:1.26E-6 ID:SGTC_2049|Compound:5262831|FD-Score:3.10|P-value:5.82E-4 ID:SGTC_2148|Compound:5546263|FD-Score:3.27|P-value:3.02E-4 ID:SGTC_2327|Compound:6189656|FD-Score:4.25|P-value:3.90E-6 ID:SGTC_2488|Compound:5268135|FD-Score:4.18|P-value:5.61E-6 ID:SGTC_2533|Compound:agelasine|FD-Score:3.23|P-value:3.59E-4 ID:SGTC_2765|Compound:myclobutanil|FD-Score:3.45|P-value:1.49E-4 ID:SGTC_2769|Compound:toremifene|FD-Score:6.93|P-value:1.50E-13 ID:SGTC_2816|Compound:7982706|FD-Score:2.96|P-value:9.68E-4 ID:SGTC_3138|Compound:9097216|FD-Score:5.21|P-value:2.11E-8 ID:SGTC_3285|Compound:9112818|FD-Score:3.12|P-value:5.39E-4 ID:SGTC_3309|Compound:9129340|FD-Score:-2.93|P-value:8.77E-4 ID:SGTC_51|Compound:1013-0126|FD-Score:4.64|P-value:5.31E-7 ID:SGTC_80|Compound:0159-0065|FD-Score:3.47|P-value:1.38E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:-3.92|P-value:1.54E-5 ID:SGTC_90|Compound:0870-0863|FD-Score:-3.39|P-value:1.51E-4 ID:SGTC_96|Compound:0929-0093|FD-Score:-3.55|P-value:7.91E-5 ID:SGTC_258|Compound:haloperidol|FD-Score:3.36|P-value:2.08E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:2.96|P-value:9.50E-4 ID:SGTC_381|Compound:3461-2219|FD-Score:3.57|P-value:9.04E-5 ID:SGTC_418|Compound:lividomycin a|FD-Score:3.25|P-value:3.23E-4 ID:SGTC_493|Compound:zm226600|FD-Score:3.17|P-value:4.42E-4 ID:SGTC_640|Compound:3381-0960|FD-Score:3.22|P-value:3.60E-4 ID:SGTC_644|Compound:1498-1197|FD-Score:3.09|P-value:5.90E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-8.45|P-value:1.69E-19 ID:SGTC_815|Compound:1582-0027|FD-Score:3.59|P-value:8.37E-5 ID:SGTC_817|Compound:1391-0715|FD-Score:4.08|P-value:8.92E-6 ID:SGTC_883|Compound:0850-0245|FD-Score:3.07|P-value:6.35E-4 ID:SGTC_1056|Compound:3448-1962|FD-Score:3.79|P-value:3.40E-5 ID:SGTC_1068|Compound:loratadine|FD-Score:3.02|P-value:7.67E-4 ID:SGTC_1087|Compound:pterostilbene|FD-Score:3.72|P-value:4.67E-5 ID:SGTC_1358|Compound:1509-0022|FD-Score:4.69|P-value:4.02E-7 ID:SGTC_1433|Compound:4060-0023|FD-Score:4.17|P-value:6.04E-6 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.05|P-value:6.96E-4 ID:SGTC_1782|Compound:st039423|FD-Score:3.47|P-value:1.35E-4 ID:SGTC_1969|Compound:st069479|FD-Score:-3.79|P-value:2.80E-5 ID:SGTC_1972|Compound:st070201|FD-Score:-3.07|P-value:5.40E-4 ID:SGTC_1979|Compound:st071809|FD-Score:4.48|P-value:1.26E-6 ID:SGTC_2049|Compound:5262831|FD-Score:3.10|P-value:5.82E-4 ID:SGTC_2148|Compound:5546263|FD-Score:3.27|P-value:3.02E-4 ID:SGTC_2327|Compound:6189656|FD-Score:4.25|P-value:3.90E-6 ID:SGTC_2488|Compound:5268135|FD-Score:4.18|P-value:5.61E-6 ID:SGTC_2533|Compound:agelasine|FD-Score:3.23|P-value:3.59E-4 ID:SGTC_2765|Compound:myclobutanil|FD-Score:3.45|P-value:1.49E-4 ID:SGTC_2769|Compound:toremifene|FD-Score:6.93|P-value:1.50E-13 ID:SGTC_2816|Compound:7982706|FD-Score:2.96|P-value:9.68E-4 ID:SGTC_3138|Compound:9097216|FD-Score:5.21|P-value:2.11E-8 ID:SGTC_3285|Compound:9112818|FD-Score:3.12|P-value:5.39E-4 ID:SGTC_3309|Compound:9129340|FD-Score:-2.93|P-value:8.77E-4

Top fitness defect scores for YNL132W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2769 toremifene 6.93 3.7 µM 1.50E-13 1 2.16E-12 1.71
2 SGTC_3138 9097216 5.21 49.5 µM 2.11E-8 1 9.38E-8 0.98
3 SGTC_1358 1509-0022 4.69 2.5 µM 4.02E-7 1 1.34E-6 0.41
4 SGTC_51 1013-0126 4.64 23.4 µM 5.31E-7 3 1.73E-6 0.35
5 SGTC_1979 st071809 4.48 45.3 µM 1.26E-6 RPP1 & pyrimidine depletion 5 3.78E-6 0.18
6 SGTC_2327 6189656 4.25 200.0 µM 3.90E-6 7 1.05E-5 0.06
7 SGTC_2488 5268135 4.18 45.7 µM 5.61E-6 3 1.45E-5 0.12
8 SGTC_1433 4060-0023 4.17 7.6 µM 6.04E-6 tubulin folding & SWR complex 1 1.55E-5 0.39
9 SGTC_817 1391-0715 4.08 46.0 µM 8.92E-6 6 2.21E-5 0.10
10 SGTC_1056 3448-1962 3.79 115.0 µM 3.40E-5 amide catabolism 9 7.42E-5 0.23
11 SGTC_1087 pterostilbene 3.72 9.1 µM 4.67E-5 7 9.89E-5 0.26
12 SGTC_815 1582-0027 3.59 36.9 µM 8.37E-5 5 1.68E-4 3.78E-4
13 SGTC_381 3461-2219 3.57 42.7 µM 9.04E-5 copper-dependent oxidative stress 3 1.80E-4 0.05
14 SGTC_1782 st039423 3.47 80.6 µM 1.35E-4 5 2.59E-4 0.09
15 SGTC_80 0159-0065 3.47 512.2 µM 1.38E-4 2 2.64E-4 0.93
16 SGTC_2765 myclobutanil 3.45 7.7 µM 1.49E-4 azole & statin 15 2.83E-4 0.01
17 SGTC_258 haloperidol 3.36 50.8 µM 2.08E-4 fatty acid desaturase (OLE1) 25 3.83E-4 0.06
18 SGTC_2148 5546263 3.27 113.6 µM 3.02E-4 7 5.37E-4 0.04
19 SGTC_418 lividomycin a 3.25 100.0 µM 3.23E-4 5 5.70E-4 0.08
20 SGTC_2533 agelasine 3.23 13.7 µM 3.59E-4 ERG2 25 6.29E-4 0.10

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.137 2.12E-15 YDL209C CWC2 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2
0.132 1.36E-14 YOR159C SME1 het Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
0.125 3.13E-13 YER136W GDI1 het GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins
0.121 2.15E-12 YEL019C MMS21 het SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination
0.120 2.47E-12 YLL035W GRC3 het Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated
0.113 5.58E-11 YCR054C CTR86 het Essential protein of unknown function; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels; relative distribution to the nucleus increases upon DNA replication stress
0.107 4.84E-10 YBR236C ABD1 het Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA
0.106 8.64E-10 YGL018C JAC1 het Specialized J-protein that functions with Hsp70 in Fe-S cluster biogenesis in mitochondria, involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix
0.105 9.37E-10 YGL097W SRM1 het Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p
0.105 1.22E-9 YPL146C NOP53 het Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired
0.104 1.32E-9 YHR164C DNA2 het Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress
0.103 2.56E-9 YBR256C RIB5 het Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway
0.099 9.50E-9 YOL130W ALR1 het Plasma membrane Mg(2+) transporter, expression and turnover are regulated by Mg(2+) concentration; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions
0.097 1.77E-8 YBR153W RIB7 het Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway
0.097 1.91E-8 YKL204W EAP1 hom eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade