YNL149C / PGA2

Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect

Zygosity: Heterozygous strain
fixedexpanded
Profile for YNL149C / PGA2

Click on Significant Values for Screen Details ID:SGTC_33|Compound:0986-0033|FD-Score:2.83|P-value:2.48E-4 ID:SGTC_68|Compound:0986-0284|FD-Score:-3.40|P-value:1.77E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:20.80|P-value:4.64E-143 ID:SGTC_137|Compound:0438-0332|FD-Score:2.85|P-value:2.24E-4 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:4.15|P-value:1.77E-7 ID:SGTC_264|Compound:promethazine|FD-Score:-3.02|P-value:1.22E-4 ID:SGTC_285|Compound:1486-1325|FD-Score:2.55|P-value:8.48E-4 ID:SGTC_315|Compound:k915-0449|FD-Score:46.00|P-value:0 ID:SGTC_319|Compound:1348-1596|FD-Score:3.08|P-value:7.50E-5 ID:SGTC_333|Compound:3486-0131|FD-Score:3.43|P-value:1.22E-5 ID:SGTC_367|Compound:0335-0881|FD-Score:3.15|P-value:5.32E-5 ID:SGTC_372|Compound:0155-0182|FD-Score:-6.82|P-value:4.81E-17 ID:SGTC_373|Compound:0844-0104|FD-Score:3.82|P-value:1.34E-6 ID:SGTC_407|Compound:eburnamonine|FD-Score:-3.30|P-value:3.03E-5 ID:SGTC_533|Compound:1222-0222|FD-Score:2.64|P-value:5.67E-4 ID:SGTC_552|Compound:k242-0005|FD-Score:4.26|P-value:8.31E-8 ID:SGTC_557|Compound:1315-0110|FD-Score:-2.97|P-value:1.58E-4 ID:SGTC_560|Compound:2332-1943|FD-Score:-2.69|P-value:5.44E-4 ID:SGTC_594|Compound:1487-1584|FD-Score:2.84|P-value:2.39E-4 ID:SGTC_642|Compound:0335-0876|FD-Score:-2.71|P-value:5.03E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:-2.69|P-value:5.56E-4 ID:SGTC_662|Compound:k832-1615|FD-Score:2.68|P-value:4.86E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-2.58|P-value:8.83E-4 ID:SGTC_815|Compound:1582-0027|FD-Score:-2.79|P-value:3.51E-4 ID:SGTC_860|Compound:0443-0016|FD-Score:4.41|P-value:3.08E-8 ID:SGTC_867|Compound:0518-0118|FD-Score:-2.57|P-value:9.26E-4 ID:SGTC_994|Compound:3910-0338|FD-Score:3.17|P-value:4.87E-5 ID:SGTC_1123|Compound:mangostin|FD-Score:-3.44|P-value:1.46E-5 ID:SGTC_1161|Compound:k007-0610|FD-Score:7.82|P-value:4.96E-22 ID:SGTC_1245|Compound:0418-0076|FD-Score:2.53|P-value:9.27E-4 ID:SGTC_1510|Compound:1123-0200|FD-Score:15.10|P-value:1.73E-76 ID:SGTC_1735|Compound:st034310|FD-Score:2.67|P-value:5.00E-4 ID:SGTC_1750|Compound:st081859|FD-Score:3.60|P-value:4.74E-6 ID:SGTC_1987|Compound:st072290|FD-Score:2.96|P-value:1.34E-4 ID:SGTC_1995|Compound:st072814|FD-Score:3.59|P-value:5.19E-6 ID:SGTC_2008|Compound:4012253|FD-Score:4.05|P-value:3.25E-7 ID:SGTC_2009|Compound:4012297|FD-Score:3.92|P-value:7.28E-7 ID:SGTC_2012|Compound:4023501|FD-Score:2.99|P-value:1.21E-4 ID:SGTC_2088|Compound:5278280|FD-Score:-2.66|P-value:6.13E-4 ID:SGTC_2115|Compound:tiliquinol|FD-Score:2.74|P-value:3.74E-4 ID:SGTC_2134|Compound:5324836|FD-Score:-3.11|P-value:8.00E-5 ID:SGTC_2157|Compound:5649910|FD-Score:3.81|P-value:1.45E-6 ID:SGTC_2246|Compound:7293507|FD-Score:2.90|P-value:1.79E-4 ID:SGTC_2430|Compound:5571206|FD-Score:4.87|P-value:1.19E-9 ID:SGTC_2455|Compound:5377712|FD-Score:-2.72|P-value:4.84E-4 ID:SGTC_2468|Compound:5987765|FD-Score:-2.91|P-value:2.02E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:-2.55|P-value:9.89E-4 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:4.53|P-value:1.37E-8 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:3.20|P-value:4.18E-5 ID:SGTC_2791|Compound:5663188|FD-Score:3.13|P-value:6.09E-5 ID:SGTC_2814|Compound:7977482|FD-Score:2.87|P-value:2.03E-4 ID:SGTC_2887|Compound:9044784|FD-Score:-2.66|P-value:6.10E-4 ID:SGTC_2888|Compound:9058547|FD-Score:-2.66|P-value:6.29E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-2.76|P-value:4.00E-4 ID:SGTC_2918|Compound:7977171|FD-Score:6.31|P-value:5.37E-15 ID:SGTC_2919|Compound:7978188|FD-Score:2.61|P-value:6.52E-4 ID:SGTC_2924|Compound:9010168|FD-Score:2.79|P-value:3.03E-4 ID:SGTC_2925|Compound:9010536|FD-Score:-3.45|P-value:1.36E-5 ID:SGTC_2945|Compound:9067681|FD-Score:-2.61|P-value:7.66E-4 ID:SGTC_2946|Compound:9065502|FD-Score:2.53|P-value:9.07E-4 ID:SGTC_2954|Compound:9074818|FD-Score:2.85|P-value:2.31E-4 ID:SGTC_2955|Compound:9076254|FD-Score:4.61|P-value:7.41E-9 ID:SGTC_2959|Compound:9079155|FD-Score:4.43|P-value:2.74E-8 ID:SGTC_2965|Compound:9082772|FD-Score:-3.37|P-value:2.12E-5 ID:SGTC_3060|Compound:1327-0027|FD-Score:2.66|P-value:5.21E-4 ID:SGTC_3066|Compound:9117245|FD-Score:-4.48|P-value:2.44E-8 ID:SGTC_3201|Compound:9113999|FD-Score:-3.63|P-value:5.02E-6 ID:SGTC_3224|Compound:9130643|FD-Score:4.51|P-value:1.53E-8 ID:SGTC_3228|Compound:9131799|FD-Score:2.88|P-value:1.98E-4 ID:SGTC_3232|Compound:9132874|FD-Score:-2.81|P-value:3.19E-4 ID:SGTC_3245|Compound:9134821|FD-Score:-2.95|P-value:1.66E-4 ID:SGTC_3290|Compound:9121031|FD-Score:-4.07|P-value:3.74E-7 ID:SGTC_3324|Compound:9138759|FD-Score:3.36|P-value:1.79E-5 ID:SGTC_33|Compound:0986-0033|FD-Score:2.83|P-value:2.48E-4 ID:SGTC_68|Compound:0986-0284|FD-Score:-3.40|P-value:1.77E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:20.80|P-value:4.64E-143 ID:SGTC_137|Compound:0438-0332|FD-Score:2.85|P-value:2.24E-4 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:4.15|P-value:1.77E-7 ID:SGTC_264|Compound:promethazine|FD-Score:-3.02|P-value:1.22E-4 ID:SGTC_285|Compound:1486-1325|FD-Score:2.55|P-value:8.48E-4 ID:SGTC_315|Compound:k915-0449|FD-Score:46.00|P-value:0 ID:SGTC_319|Compound:1348-1596|FD-Score:3.08|P-value:7.50E-5 ID:SGTC_333|Compound:3486-0131|FD-Score:3.43|P-value:1.22E-5 ID:SGTC_367|Compound:0335-0881|FD-Score:3.15|P-value:5.32E-5 ID:SGTC_372|Compound:0155-0182|FD-Score:-6.82|P-value:4.81E-17 ID:SGTC_373|Compound:0844-0104|FD-Score:3.82|P-value:1.34E-6 ID:SGTC_407|Compound:eburnamonine|FD-Score:-3.30|P-value:3.03E-5 ID:SGTC_533|Compound:1222-0222|FD-Score:2.64|P-value:5.67E-4 ID:SGTC_552|Compound:k242-0005|FD-Score:4.26|P-value:8.31E-8 ID:SGTC_557|Compound:1315-0110|FD-Score:-2.97|P-value:1.58E-4 ID:SGTC_560|Compound:2332-1943|FD-Score:-2.69|P-value:5.44E-4 ID:SGTC_594|Compound:1487-1584|FD-Score:2.84|P-value:2.39E-4 ID:SGTC_642|Compound:0335-0876|FD-Score:-2.71|P-value:5.03E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:-2.69|P-value:5.56E-4 ID:SGTC_662|Compound:k832-1615|FD-Score:2.68|P-value:4.86E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-2.58|P-value:8.83E-4 ID:SGTC_815|Compound:1582-0027|FD-Score:-2.79|P-value:3.51E-4 ID:SGTC_860|Compound:0443-0016|FD-Score:4.41|P-value:3.08E-8 ID:SGTC_867|Compound:0518-0118|FD-Score:-2.57|P-value:9.26E-4 ID:SGTC_994|Compound:3910-0338|FD-Score:3.17|P-value:4.87E-5 ID:SGTC_1123|Compound:mangostin|FD-Score:-3.44|P-value:1.46E-5 ID:SGTC_1161|Compound:k007-0610|FD-Score:7.82|P-value:4.96E-22 ID:SGTC_1245|Compound:0418-0076|FD-Score:2.53|P-value:9.27E-4 ID:SGTC_1510|Compound:1123-0200|FD-Score:15.10|P-value:1.73E-76 ID:SGTC_1735|Compound:st034310|FD-Score:2.67|P-value:5.00E-4 ID:SGTC_1750|Compound:st081859|FD-Score:3.60|P-value:4.74E-6 ID:SGTC_1987|Compound:st072290|FD-Score:2.96|P-value:1.34E-4 ID:SGTC_1995|Compound:st072814|FD-Score:3.59|P-value:5.19E-6 ID:SGTC_2008|Compound:4012253|FD-Score:4.05|P-value:3.25E-7 ID:SGTC_2009|Compound:4012297|FD-Score:3.92|P-value:7.28E-7 ID:SGTC_2012|Compound:4023501|FD-Score:2.99|P-value:1.21E-4 ID:SGTC_2088|Compound:5278280|FD-Score:-2.66|P-value:6.13E-4 ID:SGTC_2115|Compound:tiliquinol|FD-Score:2.74|P-value:3.74E-4 ID:SGTC_2134|Compound:5324836|FD-Score:-3.11|P-value:8.00E-5 ID:SGTC_2157|Compound:5649910|FD-Score:3.81|P-value:1.45E-6 ID:SGTC_2246|Compound:7293507|FD-Score:2.90|P-value:1.79E-4 ID:SGTC_2430|Compound:5571206|FD-Score:4.87|P-value:1.19E-9 ID:SGTC_2455|Compound:5377712|FD-Score:-2.72|P-value:4.84E-4 ID:SGTC_2468|Compound:5987765|FD-Score:-2.91|P-value:2.02E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:-2.55|P-value:9.89E-4 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:4.53|P-value:1.37E-8 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:3.20|P-value:4.18E-5 ID:SGTC_2791|Compound:5663188|FD-Score:3.13|P-value:6.09E-5 ID:SGTC_2814|Compound:7977482|FD-Score:2.87|P-value:2.03E-4 ID:SGTC_2887|Compound:9044784|FD-Score:-2.66|P-value:6.10E-4 ID:SGTC_2888|Compound:9058547|FD-Score:-2.66|P-value:6.29E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-2.76|P-value:4.00E-4 ID:SGTC_2918|Compound:7977171|FD-Score:6.31|P-value:5.37E-15 ID:SGTC_2919|Compound:7978188|FD-Score:2.61|P-value:6.52E-4 ID:SGTC_2924|Compound:9010168|FD-Score:2.79|P-value:3.03E-4 ID:SGTC_2925|Compound:9010536|FD-Score:-3.45|P-value:1.36E-5 ID:SGTC_2945|Compound:9067681|FD-Score:-2.61|P-value:7.66E-4 ID:SGTC_2946|Compound:9065502|FD-Score:2.53|P-value:9.07E-4 ID:SGTC_2954|Compound:9074818|FD-Score:2.85|P-value:2.31E-4 ID:SGTC_2955|Compound:9076254|FD-Score:4.61|P-value:7.41E-9 ID:SGTC_2959|Compound:9079155|FD-Score:4.43|P-value:2.74E-8 ID:SGTC_2965|Compound:9082772|FD-Score:-3.37|P-value:2.12E-5 ID:SGTC_3060|Compound:1327-0027|FD-Score:2.66|P-value:5.21E-4 ID:SGTC_3066|Compound:9117245|FD-Score:-4.48|P-value:2.44E-8 ID:SGTC_3201|Compound:9113999|FD-Score:-3.63|P-value:5.02E-6 ID:SGTC_3224|Compound:9130643|FD-Score:4.51|P-value:1.53E-8 ID:SGTC_3228|Compound:9131799|FD-Score:2.88|P-value:1.98E-4 ID:SGTC_3232|Compound:9132874|FD-Score:-2.81|P-value:3.19E-4 ID:SGTC_3245|Compound:9134821|FD-Score:-2.95|P-value:1.66E-4 ID:SGTC_3290|Compound:9121031|FD-Score:-4.07|P-value:3.74E-7 ID:SGTC_3324|Compound:9138759|FD-Score:3.36|P-value:1.79E-5

Top fitness defect scores for YNL149C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_315 k915-0449 46.00 187.0 µM 0 1 0 32.30
2 SGTC_103 0844-0104 20.80 51.8 µM 4.64E-143 ERAD & cell cycle 2 2.51E-96 5.20
3 SGTC_1510 1123-0200 15.10 71.5 µM 1.73E-76 2 8.95E-52 6.49
4 SGTC_1161 k007-0610 7.82 110.0 µM 4.96E-22 ERAD & cell cycle 11 2.71E-15 0.59
5 SGTC_2918 7977171 6.31 22.1 µM 5.37E-15 1 1.44E-10 2.33
6 SGTC_2430 5571206 4.87 200.0 µM 1.19E-9 11 5.71E-7 0.05
7 SGTC_2955 9076254 4.61 12.2 µM 7.41E-9 2 1.97E-6 0.27
8 SGTC_2573 2',4'-dihydroxychalcone 4.53 15.0 µM 1.37E-8 10 2.98E-6 0.01
9 SGTC_3224 9130643 4.51 49.5 µM 1.53E-8 RPP1 & pyrimidine depletion 6 3.21E-6 0.46
10 SGTC_2959 9079155 4.43 11.0 µM 2.74E-8 8 4.76E-6 0.41
11 SGTC_860 0443-0016 4.41 25.1 µM 3.08E-8 6 5.15E-6 0.04
12 SGTC_552 k242-0005 4.26 476.0 µM 8.31E-8 1 1.01E-5 0.52
13 SGTC_243 5hr heat shock (37°C) + splitomicin 4.15 1.0 µM 1.77E-7 heat shock/prefoldin 4 1.68E-5 0.46
14 SGTC_2008 4012253 4.05 172.0 µM 3.25E-7 13 2.53E-5 0.29
15 SGTC_2009 4012297 3.92 12.1 µM 7.28E-7 cell wall signaling 10 4.38E-5 0.02
16 SGTC_373 0844-0104 3.82 25.9 µM 1.34E-6 ERAD & cell cycle 13 6.63E-5 0.12
17 SGTC_2157 5649910 3.81 96.4 µM 1.45E-6 8 6.99E-5 0.20
18 SGTC_1750 st081859 3.60 2.4 µM 4.74E-6 endomembrane recycling 34 1.56E-4 0.00
19 SGTC_1995 st072814 3.59 61.1 µM 5.19E-6 3 1.66E-4 0.27
20 SGTC_333 3486-0131 3.43 40.1 µM 1.22E-5 amide catabolism 8 2.98E-4 0.15

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.483 7.40E-196 YNL150W_d YNL150W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking
0.273 3.53E-58 YDL148C NOP14 het Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
0.270 6.46E-57 YGR089W NNF2 hom Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation
0.234 1.05E-42 YLR065C ENV10 hom Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene
0.227 2.35E-40 YMR022W UBC7 hom Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
0.207 7.39E-34 YOL065C INP54 hom Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins
0.205 4.16E-33 YJR087W_d YJR087W_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified genes STE18 and ECM2
0.195 5.13E-30 YDL126C CDC48 het AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP
0.189 1.92E-28 YIL090W ICE2 hom Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells
0.189 2.42E-28 YMR029C FAR8 hom Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p
0.185 3.18E-27 YDR186C YDR186C hom Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.185 4.31E-27 YOR246C ENV9 hom Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
0.184 8.19E-27 YBR106W PHO88 hom Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations
0.183 1.43E-26 YOR363C PIP2 hom Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes; PIP2 has a paralog, OAF1, that arose from the whole genome duplication
0.178 2.28E-25 YLR204W QRI5 hom Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA