YNL158W / PGA1

Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes

Zygosity: Heterozygous strain
fixedexpanded
Profile for YNL158W / PGA1

Click on Significant Values for Screen Details ID:SGTC_143|Compound:4032-1194|FD-Score:4.62|P-value:1.87E-7 ID:SGTC_148|Compound:3253-0744|FD-Score:-3.28|P-value:1.62E-4 ID:SGTC_152|Compound:4469-0003|FD-Score:-3.98|P-value:6.47E-6 ID:SGTC_234|Compound:nsc-17383|FD-Score:5.11|P-value:9.60E-9 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:2.82|P-value:9.45E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:-3.07|P-value:3.85E-4 ID:SGTC_533|Compound:1222-0222|FD-Score:3.06|P-value:3.85E-4 ID:SGTC_808|Compound:0711-0032|FD-Score:3.44|P-value:7.78E-5 ID:SGTC_810|Compound:3825-7150|FD-Score:3.45|P-value:7.39E-5 ID:SGTC_901|Compound:1112-0062|FD-Score:3.13|P-value:2.82E-4 ID:SGTC_912|Compound:3474-0017|FD-Score:3.58|P-value:4.03E-5 ID:SGTC_918|Compound:glabranin|FD-Score:2.85|P-value:8.43E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-3.30|P-value:1.51E-4 ID:SGTC_1304|Compound:1165-0502|FD-Score:3.32|P-value:1.30E-4 ID:SGTC_1322|Compound:1348-1070|FD-Score:3.31|P-value:1.34E-4 ID:SGTC_1414|Compound:3917-0015|FD-Score:2.94|P-value:6.05E-4 ID:SGTC_1421|Compound:4029-0249|FD-Score:2.93|P-value:6.21E-4 ID:SGTC_1512|Compound:0259-0544|FD-Score:-3.48|P-value:6.67E-5 ID:SGTC_1520|Compound:flindersine|FD-Score:-3.87|P-value:1.11E-5 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:3.25|P-value:1.73E-4 ID:SGTC_1719|Compound:st032227|FD-Score:-4.69|P-value:1.30E-7 ID:SGTC_1722|Compound:st034304|FD-Score:-3.22|P-value:2.04E-4 ID:SGTC_1744|Compound:st037937|FD-Score:3.16|P-value:2.56E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.29|P-value:1.21E-6 ID:SGTC_1845|Compound:st056753|FD-Score:-2.83|P-value:9.61E-4 ID:SGTC_2006|Compound:4011487|FD-Score:-3.64|P-value:3.22E-5 ID:SGTC_2007|Compound:4011539|FD-Score:-3.24|P-value:1.89E-4 ID:SGTC_2112|Compound:5488546|FD-Score:3.55|P-value:4.74E-5 ID:SGTC_2316|Compound:5844804|FD-Score:-6.25|P-value:3.40E-12 ID:SGTC_2427|Compound:5545055|FD-Score:-3.11|P-value:3.27E-4 ID:SGTC_2478|Compound:5763493|FD-Score:-5.03|P-value:1.65E-8 ID:SGTC_2488|Compound:5268135|FD-Score:3.21|P-value:2.06E-4 ID:SGTC_2505|Compound:galangin|FD-Score:3.04|P-value:4.04E-4 ID:SGTC_2556|Compound:citropten|FD-Score:-3.38|P-value:1.04E-4 ID:SGTC_2560|Compound:caryophyllene|FD-Score:-4.25|P-value:1.57E-6 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:-3.15|P-value:2.74E-4 ID:SGTC_2581|Compound:avocatin a|FD-Score:3.94|P-value:7.38E-6 ID:SGTC_2587|Compound:piperic acid|FD-Score:-3.42|P-value:8.85E-5 ID:SGTC_2607|Compound:solasodine|FD-Score:3.06|P-value:3.77E-4 ID:SGTC_2750|Compound:bromebric acid|FD-Score:2.88|P-value:7.64E-4 ID:SGTC_2765|Compound:myclobutanil|FD-Score:3.21|P-value:2.05E-4 ID:SGTC_2825|Compound:7998094|FD-Score:4.67|P-value:1.41E-7 ID:SGTC_2910|Compound:7948405|FD-Score:3.35|P-value:1.15E-4 ID:SGTC_3020|Compound:9089140|FD-Score:5.57|P-value:4.44E-10 ID:SGTC_3055|Compound:9094636|FD-Score:-3.09|P-value:3.56E-4 ID:SGTC_3065|Compound:9113888|FD-Score:-3.81|P-value:1.44E-5 ID:SGTC_3070|Compound:9117005|FD-Score:4.10|P-value:3.20E-6 ID:SGTC_3101|Compound:9120531|FD-Score:3.01|P-value:4.54E-4 ID:SGTC_3106|Compound:9121286|FD-Score:2.83|P-value:9.17E-4 ID:SGTC_3108|Compound:9121785|FD-Score:3.03|P-value:4.35E-4 ID:SGTC_3224|Compound:9130643|FD-Score:2.83|P-value:9.30E-4 ID:SGTC_3285|Compound:9112818|FD-Score:-3.36|P-value:1.13E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:4.62|P-value:1.87E-7 ID:SGTC_148|Compound:3253-0744|FD-Score:-3.28|P-value:1.62E-4 ID:SGTC_152|Compound:4469-0003|FD-Score:-3.98|P-value:6.47E-6 ID:SGTC_234|Compound:nsc-17383|FD-Score:5.11|P-value:9.60E-9 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:2.82|P-value:9.45E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:-3.07|P-value:3.85E-4 ID:SGTC_533|Compound:1222-0222|FD-Score:3.06|P-value:3.85E-4 ID:SGTC_808|Compound:0711-0032|FD-Score:3.44|P-value:7.78E-5 ID:SGTC_810|Compound:3825-7150|FD-Score:3.45|P-value:7.39E-5 ID:SGTC_901|Compound:1112-0062|FD-Score:3.13|P-value:2.82E-4 ID:SGTC_912|Compound:3474-0017|FD-Score:3.58|P-value:4.03E-5 ID:SGTC_918|Compound:glabranin|FD-Score:2.85|P-value:8.43E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-3.30|P-value:1.51E-4 ID:SGTC_1304|Compound:1165-0502|FD-Score:3.32|P-value:1.30E-4 ID:SGTC_1322|Compound:1348-1070|FD-Score:3.31|P-value:1.34E-4 ID:SGTC_1414|Compound:3917-0015|FD-Score:2.94|P-value:6.05E-4 ID:SGTC_1421|Compound:4029-0249|FD-Score:2.93|P-value:6.21E-4 ID:SGTC_1512|Compound:0259-0544|FD-Score:-3.48|P-value:6.67E-5 ID:SGTC_1520|Compound:flindersine|FD-Score:-3.87|P-value:1.11E-5 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:3.25|P-value:1.73E-4 ID:SGTC_1719|Compound:st032227|FD-Score:-4.69|P-value:1.30E-7 ID:SGTC_1722|Compound:st034304|FD-Score:-3.22|P-value:2.04E-4 ID:SGTC_1744|Compound:st037937|FD-Score:3.16|P-value:2.56E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.29|P-value:1.21E-6 ID:SGTC_1845|Compound:st056753|FD-Score:-2.83|P-value:9.61E-4 ID:SGTC_2006|Compound:4011487|FD-Score:-3.64|P-value:3.22E-5 ID:SGTC_2007|Compound:4011539|FD-Score:-3.24|P-value:1.89E-4 ID:SGTC_2112|Compound:5488546|FD-Score:3.55|P-value:4.74E-5 ID:SGTC_2316|Compound:5844804|FD-Score:-6.25|P-value:3.40E-12 ID:SGTC_2427|Compound:5545055|FD-Score:-3.11|P-value:3.27E-4 ID:SGTC_2478|Compound:5763493|FD-Score:-5.03|P-value:1.65E-8 ID:SGTC_2488|Compound:5268135|FD-Score:3.21|P-value:2.06E-4 ID:SGTC_2505|Compound:galangin|FD-Score:3.04|P-value:4.04E-4 ID:SGTC_2556|Compound:citropten|FD-Score:-3.38|P-value:1.04E-4 ID:SGTC_2560|Compound:caryophyllene|FD-Score:-4.25|P-value:1.57E-6 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:-3.15|P-value:2.74E-4 ID:SGTC_2581|Compound:avocatin a|FD-Score:3.94|P-value:7.38E-6 ID:SGTC_2587|Compound:piperic acid|FD-Score:-3.42|P-value:8.85E-5 ID:SGTC_2607|Compound:solasodine|FD-Score:3.06|P-value:3.77E-4 ID:SGTC_2750|Compound:bromebric acid|FD-Score:2.88|P-value:7.64E-4 ID:SGTC_2765|Compound:myclobutanil|FD-Score:3.21|P-value:2.05E-4 ID:SGTC_2825|Compound:7998094|FD-Score:4.67|P-value:1.41E-7 ID:SGTC_2910|Compound:7948405|FD-Score:3.35|P-value:1.15E-4 ID:SGTC_3020|Compound:9089140|FD-Score:5.57|P-value:4.44E-10 ID:SGTC_3055|Compound:9094636|FD-Score:-3.09|P-value:3.56E-4 ID:SGTC_3065|Compound:9113888|FD-Score:-3.81|P-value:1.44E-5 ID:SGTC_3070|Compound:9117005|FD-Score:4.10|P-value:3.20E-6 ID:SGTC_3101|Compound:9120531|FD-Score:3.01|P-value:4.54E-4 ID:SGTC_3106|Compound:9121286|FD-Score:2.83|P-value:9.17E-4 ID:SGTC_3108|Compound:9121785|FD-Score:3.03|P-value:4.35E-4 ID:SGTC_3224|Compound:9130643|FD-Score:2.83|P-value:9.30E-4 ID:SGTC_3285|Compound:9112818|FD-Score:-3.36|P-value:1.13E-4

Top fitness defect scores for YNL158W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_3020 9089140 5.57 49.5 µM 4.44E-10 4 1.25E-8 0.55
2 SGTC_234 nsc-17383 5.11 10.7 µM 9.60E-9 ERG2 1 1.62E-7 0.72
3 SGTC_2825 7998094 4.67 58.4 µM 1.41E-7 fatty acid desaturase (OLE1) 7 1.51E-6 0.01
4 SGTC_143 4032-1194 4.62 15.7 µM 1.87E-7 10 1.91E-6 0.12
5 SGTC_1844 rapamycin 4.29 4.0 nM 1.21E-6 46 9.12E-6 0.10
6 SGTC_3070 9117005 4.10 49.5 µM 3.20E-6 3 2.04E-5 0.56
7 SGTC_2581 avocatin a 3.94 64.0 µM 7.38E-6 sphingolipid biosynthesis & PDR1 7 4.11E-5 0.16
8 SGTC_912 3474-0017 3.58 2.2 µM 4.03E-5 7 1.70E-4 0.11
9 SGTC_2112 5488546 3.55 200.0 µM 4.74E-5 TSC3-RPN4 10 1.95E-4 6.56E-4
10 SGTC_810 3825-7150 3.45 143.0 µM 7.39E-5 60S ribosome export 4 2.82E-4 0.32
11 SGTC_808 0711-0032 3.44 135.0 µM 7.78E-5 14 2.94E-4 0.04
12 SGTC_2910 7948405 3.35 26.0 µM 1.15E-4 plasma membrane duress 7 4.07E-4 0.19
13 SGTC_1304 1165-0502 3.32 251.0 µM 1.30E-4 11 4.53E-4 0.00
14 SGTC_1322 1348-1070 3.31 12.0 µM 1.34E-4 7 4.65E-4 0.42
15 SGTC_1551 6-bromo-3'-methylflavone 3.25 63.5 µM 1.73E-4 5 5.75E-4 0.03
16 SGTC_2765 myclobutanil 3.21 7.7 µM 2.05E-4 azole & statin 21 6.62E-4 0.02
17 SGTC_2488 5268135 3.21 45.7 µM 2.06E-4 17 6.63E-4 0.01
18 SGTC_1744 st037937 3.16 38.9 µM 2.56E-4 12 7.98E-4 0.10
19 SGTC_901 1112-0062 3.13 60.1 µM 2.82E-4 12 8.64E-4 0.11
20 SGTC_2607 solasodine 3.06 47.4 µM 3.77E-4 29 0.00110 0.05

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.115 2.47E-11 YJR151W-A_p YJR151W-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR; predicted to have a role in transcription based on computational guilt by association analysis
0.112 9.47E-11 YJR066W TOR1 hom PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.108 3.69E-10 YNL119W NCS2 hom Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.104 1.49E-9 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.104 1.54E-9 YLR114C AVL9 hom Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.100 6.58E-9 YML121W GTR1 hom Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
0.097 1.53E-8 YLR209C PNP1 hom Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway
0.095 4.20E-8 YFL024C EPL1 het Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb
0.092 9.45E-8 YDR267C CIA1 het Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein
0.090 2.06E-7 YHR186C KOG1 het Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
0.087 5.09E-7 YNL032W SIW14 hom Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm
0.083 1.71E-6 YOR069W VPS5 hom Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes
0.083 1.70E-6 YGR017W_p YGR017W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm
0.081 2.67E-6 YKR060W UTP30 hom Putative subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit
0.081 2.81E-6 YJL164C TPK1 hom cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication