YNL172W / APC1

Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YNL172W / APC1

Click on Significant Values for Screen Details ID:SGTC_11|Compound:0438-0307|FD-Score:4.39|P-value:1.30E-7 ID:SGTC_30|Compound:5-(2-thienylidene)-Rhodanine|FD-Score:3.19|P-value:9.41E-5 ID:SGTC_35|Compound:0133-0054|FD-Score:5.72|P-value:9.74E-12 ID:SGTC_38|Compound:0929-0051|FD-Score:4.24|P-value:3.39E-7 ID:SGTC_40|Compound:0327-0103|FD-Score:-5.98|P-value:8.71E-13 ID:SGTC_50|Compound:0929-0078|FD-Score:2.87|P-value:3.96E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.21|P-value:7.40E-5 ID:SGTC_264|Compound:promethazine|FD-Score:2.81|P-value:4.98E-4 ID:SGTC_271|Compound:rotenone|FD-Score:4.12|P-value:6.66E-7 ID:SGTC_535|Compound:2293-4129|FD-Score:2.74|P-value:6.82E-4 ID:SGTC_561|Compound:1550-0330|FD-Score:2.88|P-value:3.70E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:2.66|P-value:9.35E-4 ID:SGTC_1013|Compound:4112-3890|FD-Score:-2.85|P-value:3.78E-4 ID:SGTC_1037|Compound:k284-1091|FD-Score:-3.42|P-value:2.64E-5 ID:SGTC_1066|Compound:ketoconazole|FD-Score:3.02|P-value:2.05E-4 ID:SGTC_1093|Compound:fluphenazine|FD-Score:2.85|P-value:4.27E-4 ID:SGTC_1362|Compound:1611-0397|FD-Score:3.68|P-value:8.00E-6 ID:SGTC_1505|Compound:k297-0037|FD-Score:4.63|P-value:2.72E-8 ID:SGTC_1688|Compound:st021091|FD-Score:3.40|P-value:3.38E-5 ID:SGTC_1768|Compound:st044301|FD-Score:-2.80|P-value:4.67E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:2.70|P-value:7.77E-4 ID:SGTC_1948|Compound:st076329|FD-Score:3.25|P-value:7.00E-5 ID:SGTC_1949|Compound:st076024|FD-Score:3.45|P-value:2.70E-5 ID:SGTC_2149|Compound:5554786|FD-Score:-2.75|P-value:5.86E-4 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:3.64|P-value:9.70E-6 ID:SGTC_2456|Compound:5770359|FD-Score:4.49|P-value:7.04E-8 ID:SGTC_2459|Compound:5811157|FD-Score:-3.34|P-value:3.98E-5 ID:SGTC_2465|Compound:5809242|FD-Score:-2.81|P-value:4.54E-4 ID:SGTC_2796|Compound:7949634|FD-Score:-2.75|P-value:5.67E-4 ID:SGTC_2835|Compound:9006588|FD-Score:-2.89|P-value:3.17E-4 ID:SGTC_2848|Compound:9016370|FD-Score:4.59|P-value:3.61E-8 ID:SGTC_2940|Compound:9060638|FD-Score:-3.83|P-value:3.03E-6 ID:SGTC_3001|Compound:9076041|FD-Score:3.36|P-value:4.22E-5 ID:SGTC_3006|Compound:9075424|FD-Score:-2.77|P-value:5.33E-4 ID:SGTC_3164|Compound:9100705|FD-Score:3.06|P-value:1.67E-4 ID:SGTC_3177|Compound:9107506|FD-Score:3.24|P-value:7.43E-5 ID:SGTC_3220|Compound:9131269|FD-Score:-2.89|P-value:3.13E-4 ID:SGTC_3244|Compound:9134489|FD-Score:3.51|P-value:2.00E-5 ID:SGTC_3327|Compound:9140648|FD-Score:-2.72|P-value:6.53E-4 ID:SGTC_3329|Compound:9141233|FD-Score:3.04|P-value:1.84E-4 ID:SGTC_11|Compound:0438-0307|FD-Score:4.39|P-value:1.30E-7 ID:SGTC_30|Compound:5-(2-thienylidene)-Rhodanine|FD-Score:3.19|P-value:9.41E-5 ID:SGTC_35|Compound:0133-0054|FD-Score:5.72|P-value:9.74E-12 ID:SGTC_38|Compound:0929-0051|FD-Score:4.24|P-value:3.39E-7 ID:SGTC_40|Compound:0327-0103|FD-Score:-5.98|P-value:8.71E-13 ID:SGTC_50|Compound:0929-0078|FD-Score:2.87|P-value:3.96E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.21|P-value:7.40E-5 ID:SGTC_264|Compound:promethazine|FD-Score:2.81|P-value:4.98E-4 ID:SGTC_271|Compound:rotenone|FD-Score:4.12|P-value:6.66E-7 ID:SGTC_535|Compound:2293-4129|FD-Score:2.74|P-value:6.82E-4 ID:SGTC_561|Compound:1550-0330|FD-Score:2.88|P-value:3.70E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:2.66|P-value:9.35E-4 ID:SGTC_1013|Compound:4112-3890|FD-Score:-2.85|P-value:3.78E-4 ID:SGTC_1037|Compound:k284-1091|FD-Score:-3.42|P-value:2.64E-5 ID:SGTC_1066|Compound:ketoconazole|FD-Score:3.02|P-value:2.05E-4 ID:SGTC_1093|Compound:fluphenazine|FD-Score:2.85|P-value:4.27E-4 ID:SGTC_1362|Compound:1611-0397|FD-Score:3.68|P-value:8.00E-6 ID:SGTC_1505|Compound:k297-0037|FD-Score:4.63|P-value:2.72E-8 ID:SGTC_1688|Compound:st021091|FD-Score:3.40|P-value:3.38E-5 ID:SGTC_1768|Compound:st044301|FD-Score:-2.80|P-value:4.67E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:2.70|P-value:7.77E-4 ID:SGTC_1948|Compound:st076329|FD-Score:3.25|P-value:7.00E-5 ID:SGTC_1949|Compound:st076024|FD-Score:3.45|P-value:2.70E-5 ID:SGTC_2149|Compound:5554786|FD-Score:-2.75|P-value:5.86E-4 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:3.64|P-value:9.70E-6 ID:SGTC_2456|Compound:5770359|FD-Score:4.49|P-value:7.04E-8 ID:SGTC_2459|Compound:5811157|FD-Score:-3.34|P-value:3.98E-5 ID:SGTC_2465|Compound:5809242|FD-Score:-2.81|P-value:4.54E-4 ID:SGTC_2796|Compound:7949634|FD-Score:-2.75|P-value:5.67E-4 ID:SGTC_2835|Compound:9006588|FD-Score:-2.89|P-value:3.17E-4 ID:SGTC_2848|Compound:9016370|FD-Score:4.59|P-value:3.61E-8 ID:SGTC_2940|Compound:9060638|FD-Score:-3.83|P-value:3.03E-6 ID:SGTC_3001|Compound:9076041|FD-Score:3.36|P-value:4.22E-5 ID:SGTC_3006|Compound:9075424|FD-Score:-2.77|P-value:5.33E-4 ID:SGTC_3164|Compound:9100705|FD-Score:3.06|P-value:1.67E-4 ID:SGTC_3177|Compound:9107506|FD-Score:3.24|P-value:7.43E-5 ID:SGTC_3220|Compound:9131269|FD-Score:-2.89|P-value:3.13E-4 ID:SGTC_3244|Compound:9134489|FD-Score:3.51|P-value:2.00E-5 ID:SGTC_3327|Compound:9140648|FD-Score:-2.72|P-value:6.53E-4 ID:SGTC_3329|Compound:9141233|FD-Score:3.04|P-value:1.84E-4

Top fitness defect scores for YNL172W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_35 0133-0054 5.72 64.1 µM 9.74E-12 1 5.43E-9 0.72
2 SGTC_1505 k297-0037 4.63 20.6 µM 2.72E-8 2 1.79E-6 0.29
3 SGTC_2848 9016370 4.59 26.0 µM 3.61E-8 1 2.20E-6 0.38
4 SGTC_2456 5770359 4.49 74.5 µM 7.04E-8 2 3.60E-6 0.45
5 SGTC_11 0438-0307 4.39 1.3 µM 1.30E-7 4 5.62E-6 0.40
6 SGTC_38 0929-0051 4.24 58.3 µM 3.39E-7 4 1.14E-5 0.43
7 SGTC_271 rotenone 4.12 740.0 µM 6.66E-7 1 1.87E-5 0.72
8 SGTC_1362 1611-0397 3.68 101.0 µM 8.00E-6 4 1.16E-4 0.03
9 SGTC_2454 5-benzylidenerhodanine 3.64 10.4 µM 9.70E-6 TRP & mitochondrial translation 7 1.34E-4 0.11
10 SGTC_3244 9134489 3.51 49.5 µM 2.00E-5 7 2.28E-4 0.08
11 SGTC_1949 st076024 3.45 69.1 µM 2.70E-5 12 2.85E-4 0.10
12 SGTC_1688 st021091 3.40 57.6 µM 3.38E-5 6 3.37E-4 0.39
13 SGTC_3001 9076041 3.36 71.4 µM 4.22E-5 8 3.96E-4 0.01
14 SGTC_1948 st076329 3.25 66.6 µM 7.00E-5 11 5.76E-4 0.17
15 SGTC_3177 9107506 3.24 49.5 µM 7.43E-5 ERG2 3 6.02E-4 0.17
16 SGTC_30 5-(2-thienylidene)-Rhodanine 3.19 28.7 µM 9.41E-5 5 7.17E-4 0.15
17 SGTC_3164 9100705 3.06 49.5 µM 1.67E-4 3 0.00110 0.26
18 SGTC_3329 9141233 3.04 27.6 µM 1.84E-4 8 0.00118 0.16
19 SGTC_1066 ketoconazole 3.02 628.0 nM 2.05E-4 9 0.00128 0.05
20 SGTC_561 1550-0330 2.88 72.9 µM 3.70E-4 15 0.00198 0.09

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.109 2.77E-10 YDR454C GUK1 het Guanylate kinase, converts GMP to GDP; required for growth and mannose outer chain elongation of cell wall N-linked glycoproteins
0.106 7.19E-10 YLR342W-A_p YLR342W-A_p hom Putative protein of unknown function
0.094 5.65E-8 YAL002W VPS8 hom Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif
0.092 8.02E-8 YGL098W USE1 het Essential SNARE protein localized to the ER, involved in retrograde traffic from the Golgi to the ER; forms a complex with the SNAREs Sec22p, Sec20p and Ufe1p
0.090 2.03E-7 YLR396C VPS33 hom ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essential for protein sorting, vesicle docking and fusion at the vacuole
0.088 3.58E-7 YDR327W_d YDR327W_d het Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1
0.083 1.46E-6 YKL074C MUD2 hom Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65
0.083 1.64E-6 YDL125C HNT1 hom Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress
0.082 1.77E-6 YOL079W_d YOL079W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.082 1.80E-6 YDR104C SPO71 hom Meiosis-specific protein of unknown function, required for spore wall formation during sporulation, in particular for the proper size of the prospore membrane (PSM); interacts with SPO1; dispensable for both nuclear divisions during meiosis; contains two PH domains
0.082 2.13E-6 YDL179W PCL9 hom Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p
0.081 2.35E-6 YDR045C RPC11 het RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS
0.079 4.25E-6 YDR408C ADE8 hom Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway
0.079 4.40E-6 YNR030W ALG12 hom Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation
0.079 4.43E-6 YOL071W EMI5 hom Subunit of succinate dehydrogenase, which couples succinate oxidation to ubiquinone reduction; required for FAD cofactor attachment to Sdh1p; mutations in human ortholog PGL2 are associated with neuroendocrine tumors (paraganglioma)