YNL182C / IPI3

Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; highly conserved protein which contains several WD40 motifs; IPI3 is an essential gene

Zygosity: Heterozygous strain
fixedexpanded
Profile for YNL182C / IPI3

Click on Significant Values for Screen Details ID:SGTC_69|Compound:0096-0274|FD-Score:3.32|P-value:1.67E-4 ID:SGTC_126|Compound:0417-1624|FD-Score:-3.02|P-value:5.63E-4 ID:SGTC_135|Compound:0933-0004|FD-Score:4.50|P-value:6.02E-7 ID:SGTC_164|Compound:4100-3994|FD-Score:2.96|P-value:6.94E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:5.53|P-value:1.16E-9 ID:SGTC_513|Compound:4245-1575|FD-Score:4.14|P-value:3.96E-6 ID:SGTC_521|Compound:r062-0005|FD-Score:3.24|P-value:2.37E-4 ID:SGTC_535|Compound:2293-4129|FD-Score:2.94|P-value:7.66E-4 ID:SGTC_570|Compound:0987-0065|FD-Score:4.42|P-value:9.06E-7 ID:SGTC_579|Compound:4491-1009|FD-Score:3.14|P-value:3.55E-4 ID:SGTC_618|Compound:k072-0232|FD-Score:3.12|P-value:3.81E-4 ID:SGTC_634|Compound:1611-4317|FD-Score:4.07|P-value:5.53E-6 ID:SGTC_750|Compound:4073-0104|FD-Score:-2.92|P-value:8.02E-4 ID:SGTC_766|Compound:1391-0721|FD-Score:3.30|P-value:1.87E-4 ID:SGTC_953|Compound:1188-0045|FD-Score:3.47|P-value:8.87E-5 ID:SGTC_976|Compound:1348-1067|FD-Score:5.97|P-value:6.01E-11 ID:SGTC_1093|Compound:fluphenazine|FD-Score:4.03|P-value:6.71E-6 ID:SGTC_1196|Compound:1588-0294|FD-Score:2.96|P-value:6.95E-4 ID:SGTC_1348|Compound:1493-0373|FD-Score:-3.76|P-value:2.46E-5 ID:SGTC_1349|Compound:flufenamic acid|FD-Score:-3.19|P-value:2.89E-4 ID:SGTC_1358|Compound:1509-0022|FD-Score:-2.97|P-value:6.75E-4 ID:SGTC_1374|Compound:2889-5571|FD-Score:-2.87|P-value:9.89E-4 ID:SGTC_1524|Compound:corynanthine|FD-Score:3.01|P-value:5.74E-4 ID:SGTC_1542|Compound:7-hydroxy-3'-methoxyflavone|FD-Score:4.10|P-value:4.80E-6 ID:SGTC_1732|Compound:st034308|FD-Score:-3.31|P-value:1.74E-4 ID:SGTC_1783|Compound:st039459|FD-Score:3.26|P-value:2.20E-4 ID:SGTC_1823|Compound:3-hydroxy-2-naphthoic acid|FD-Score:-3.18|P-value:2.92E-4 ID:SGTC_1849|Compound:st056239|FD-Score:3.38|P-value:1.34E-4 ID:SGTC_1932|Compound:st074806|FD-Score:3.74|P-value:2.69E-5 ID:SGTC_2095|Compound:5432005|FD-Score:3.23|P-value:2.50E-4 ID:SGTC_2198|Compound:6589944|FD-Score:4.20|P-value:2.87E-6 ID:SGTC_2207|Compound:7258803|FD-Score:3.12|P-value:3.73E-4 ID:SGTC_2307|Compound:7538926|FD-Score:3.10|P-value:4.15E-4 ID:SGTC_2325|Compound:6158266|FD-Score:3.05|P-value:4.96E-4 ID:SGTC_2347|Compound:9049783|FD-Score:3.37|P-value:1.37E-4 ID:SGTC_2379|Compound:9035213|FD-Score:3.69|P-value:3.45E-5 ID:SGTC_2397|Compound:5161689|FD-Score:7.03|P-value:1.67E-14 ID:SGTC_2552|Compound:juarezic acid|FD-Score:3.66|P-value:3.97E-5 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-3.04|P-value:5.13E-4 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:-4.38|P-value:1.10E-6 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:7.84|P-value:1.36E-17 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:-2.98|P-value:6.48E-4 ID:SGTC_2691|Compound:st077356|FD-Score:4.73|P-value:1.62E-7 ID:SGTC_2802|Compound:7925815|FD-Score:3.57|P-value:5.94E-5 ID:SGTC_2954|Compound:9074818|FD-Score:3.47|P-value:8.99E-5 ID:SGTC_2959|Compound:9079155|FD-Score:3.70|P-value:3.20E-5 ID:SGTC_2986|Compound:9022445|FD-Score:3.29|P-value:1.92E-4 ID:SGTC_3026|Compound:9090633|FD-Score:3.49|P-value:8.42E-5 ID:SGTC_3028|Compound:9087778|FD-Score:-3.06|P-value:4.78E-4 ID:SGTC_3063|Compound:1327-0034|FD-Score:3.07|P-value:4.65E-4 ID:SGTC_3173|Compound:9105693|FD-Score:-3.36|P-value:1.40E-4 ID:SGTC_3180|Compound:9109287|FD-Score:3.44|P-value:1.04E-4 ID:SGTC_3187|Compound:9109394|FD-Score:3.63|P-value:4.42E-5 ID:SGTC_69|Compound:0096-0274|FD-Score:3.32|P-value:1.67E-4 ID:SGTC_126|Compound:0417-1624|FD-Score:-3.02|P-value:5.63E-4 ID:SGTC_135|Compound:0933-0004|FD-Score:4.50|P-value:6.02E-7 ID:SGTC_164|Compound:4100-3994|FD-Score:2.96|P-value:6.94E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:5.53|P-value:1.16E-9 ID:SGTC_513|Compound:4245-1575|FD-Score:4.14|P-value:3.96E-6 ID:SGTC_521|Compound:r062-0005|FD-Score:3.24|P-value:2.37E-4 ID:SGTC_535|Compound:2293-4129|FD-Score:2.94|P-value:7.66E-4 ID:SGTC_570|Compound:0987-0065|FD-Score:4.42|P-value:9.06E-7 ID:SGTC_579|Compound:4491-1009|FD-Score:3.14|P-value:3.55E-4 ID:SGTC_618|Compound:k072-0232|FD-Score:3.12|P-value:3.81E-4 ID:SGTC_634|Compound:1611-4317|FD-Score:4.07|P-value:5.53E-6 ID:SGTC_750|Compound:4073-0104|FD-Score:-2.92|P-value:8.02E-4 ID:SGTC_766|Compound:1391-0721|FD-Score:3.30|P-value:1.87E-4 ID:SGTC_953|Compound:1188-0045|FD-Score:3.47|P-value:8.87E-5 ID:SGTC_976|Compound:1348-1067|FD-Score:5.97|P-value:6.01E-11 ID:SGTC_1093|Compound:fluphenazine|FD-Score:4.03|P-value:6.71E-6 ID:SGTC_1196|Compound:1588-0294|FD-Score:2.96|P-value:6.95E-4 ID:SGTC_1348|Compound:1493-0373|FD-Score:-3.76|P-value:2.46E-5 ID:SGTC_1349|Compound:flufenamic acid|FD-Score:-3.19|P-value:2.89E-4 ID:SGTC_1358|Compound:1509-0022|FD-Score:-2.97|P-value:6.75E-4 ID:SGTC_1374|Compound:2889-5571|FD-Score:-2.87|P-value:9.89E-4 ID:SGTC_1524|Compound:corynanthine|FD-Score:3.01|P-value:5.74E-4 ID:SGTC_1542|Compound:7-hydroxy-3'-methoxyflavone|FD-Score:4.10|P-value:4.80E-6 ID:SGTC_1732|Compound:st034308|FD-Score:-3.31|P-value:1.74E-4 ID:SGTC_1783|Compound:st039459|FD-Score:3.26|P-value:2.20E-4 ID:SGTC_1823|Compound:3-hydroxy-2-naphthoic acid|FD-Score:-3.18|P-value:2.92E-4 ID:SGTC_1849|Compound:st056239|FD-Score:3.38|P-value:1.34E-4 ID:SGTC_1932|Compound:st074806|FD-Score:3.74|P-value:2.69E-5 ID:SGTC_2095|Compound:5432005|FD-Score:3.23|P-value:2.50E-4 ID:SGTC_2198|Compound:6589944|FD-Score:4.20|P-value:2.87E-6 ID:SGTC_2207|Compound:7258803|FD-Score:3.12|P-value:3.73E-4 ID:SGTC_2307|Compound:7538926|FD-Score:3.10|P-value:4.15E-4 ID:SGTC_2325|Compound:6158266|FD-Score:3.05|P-value:4.96E-4 ID:SGTC_2347|Compound:9049783|FD-Score:3.37|P-value:1.37E-4 ID:SGTC_2379|Compound:9035213|FD-Score:3.69|P-value:3.45E-5 ID:SGTC_2397|Compound:5161689|FD-Score:7.03|P-value:1.67E-14 ID:SGTC_2552|Compound:juarezic acid|FD-Score:3.66|P-value:3.97E-5 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-3.04|P-value:5.13E-4 ID:SGTC_2580|Compound:diallyl disulphide|FD-Score:-4.38|P-value:1.10E-6 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:7.84|P-value:1.36E-17 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:-2.98|P-value:6.48E-4 ID:SGTC_2691|Compound:st077356|FD-Score:4.73|P-value:1.62E-7 ID:SGTC_2802|Compound:7925815|FD-Score:3.57|P-value:5.94E-5 ID:SGTC_2954|Compound:9074818|FD-Score:3.47|P-value:8.99E-5 ID:SGTC_2959|Compound:9079155|FD-Score:3.70|P-value:3.20E-5 ID:SGTC_2986|Compound:9022445|FD-Score:3.29|P-value:1.92E-4 ID:SGTC_3026|Compound:9090633|FD-Score:3.49|P-value:8.42E-5 ID:SGTC_3028|Compound:9087778|FD-Score:-3.06|P-value:4.78E-4 ID:SGTC_3063|Compound:1327-0034|FD-Score:3.07|P-value:4.65E-4 ID:SGTC_3173|Compound:9105693|FD-Score:-3.36|P-value:1.40E-4 ID:SGTC_3180|Compound:9109287|FD-Score:3.44|P-value:1.04E-4 ID:SGTC_3187|Compound:9109394|FD-Score:3.63|P-value:4.42E-5

Top fitness defect scores for YNL182C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2590 3,4',5',6',7'-pentamethoxyflavone 7.84 100.0 µM 1.36E-17 60S ribosome export 3 2.32E-15 1.11
2 SGTC_2397 5161689 7.03 200.0 µM 1.67E-14 1 1.06E-12 2.31
3 SGTC_976 1348-1067 5.97 47.6 µM 6.01E-11 60S ribosome export 2 1.22E-9 0.06
4 SGTC_309 k408-0051 5.53 1.5 µM 1.16E-9 10 1.56E-8 0.05
5 SGTC_2691 st077356 4.73 60.2 µM 1.62E-7 7 1.10E-6 0.16
6 SGTC_135 0933-0004 4.50 26.4 µM 6.02E-7 60S ribosome export 4 3.42E-6 0.01
7 SGTC_570 0987-0065 4.42 62.6 µM 9.06E-7 60S ribosome export 5 4.87E-6 0.54
8 SGTC_2198 6589944 4.20 200.0 µM 2.87E-6 2 1.32E-5 0.36
9 SGTC_513 4245-1575 4.14 41.7 µM 3.96E-6 unfolded protein response 26 1.74E-5 0.11
10 SGTC_1542 7-hydroxy-3'-methoxyflavone 4.10 49.7 µM 4.80E-6 3 2.06E-5 0.00
11 SGTC_634 1611-4317 4.07 11.2 µM 5.53E-6 ERG2 7 2.32E-5 0.02
12 SGTC_1093 fluphenazine 4.03 8.0 µM 6.71E-6 4 2.75E-5 0.02
13 SGTC_1932 st074806 3.74 40.9 µM 2.69E-5 8 9.12E-5 0.10
14 SGTC_2959 9079155 3.70 11.0 µM 3.20E-5 11 1.06E-4 0.04
15 SGTC_2379 9035213 3.69 200.0 µM 3.45E-5 6 1.13E-4 0.02
16 SGTC_2552 juarezic acid 3.66 87.0 µM 3.97E-5 TSC3-RPN4 5 1.28E-4 0.43
17 SGTC_3187 9109394 3.63 49.5 µM 4.42E-5 Golgi 14 1.40E-4 0.10
18 SGTC_2802 7925815 3.57 54.5 µM 5.94E-5 13 1.81E-4 0.26
19 SGTC_3026 9090633 3.49 49.5 µM 8.42E-5 11 2.45E-4 0.03
20 SGTC_953 1188-0045 3.47 9.7 µM 8.87E-5 60S ribosome export 12 2.56E-4 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.143 7.99E-17 YGR187C HGH1 hom Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress
0.140 3.56E-16 YOL053W AIM39 hom Putative protein of unknown function; null mutant displays elevated frequency of mitochondrial genome loss
0.132 1.50E-14 YJL153C INO1 hom Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element
0.129 7.35E-14 YKL111C_d YKL111C_d het Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene ABF1
0.123 7.91E-13 YBR072W HSP26 hom Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity
0.118 6.25E-12 YGL099W LSG1 het Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
0.118 7.61E-12 YPR012W_d YPR012W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene
0.117 9.81E-12 YFR032C-B_p YFR032C-B_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.096 2.42E-8 YIL046W MET30 het F-box protein containing five copies of the WD40 motif, controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus
0.091 1.33E-7 YLR046C_p YLR046C_p hom Putative membrane protein; member of the fungal lipid-translocating exporter (LTE) family of proteins; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance
0.089 2.28E-7 YIL131C FKH1 hom Forkhead family transcription factor; has a minor role in the expression of G2/M phase genes; negatively regulates transcriptional elongation; positive role in chromatin silencing at HML and HMR; binds to the recombination enhancer near HML, and regulates donor preference during mating-type switching by positioning HML near the MAT locus through FHA-domain mediated interactions with proteins near the DSB; FKH1 has a paralog, FKH2, that arose from the whole genome duplication
0.089 2.96E-7 YDR371W_p CTS2_p hom Putative chitinase, functionally complements A. gossypii cts2 mutant sporulation defect
0.082 2.29E-6 YOR092W ECM3 hom Non-essential protein of unknown function; involved in signal transduction and the genotoxic response; induced rapidly in response to treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from ER to cytoplasm upon DNA replication stress; ECM3 has a paralog, YNL095C, that arose from the whole genome duplication
0.082 2.17E-6 YPL250C ICY2 hom Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate
0.082 2.17E-6 YKR092C SRP40 hom Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140