YNL188W / KAR1

Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p

Zygosity: Heterozygous strain
fixedexpanded
Profile for YNL188W / KAR1

Click on Significant Values for Screen Details ID:SGTC_35|Compound:0133-0054|FD-Score:-5.01|P-value:3.21E-7 ID:SGTC_42|Compound:0133-0086|FD-Score:-5.14|P-value:1.60E-7 ID:SGTC_335|Compound:4077-0256|FD-Score:-3.80|P-value:8.03E-5 ID:SGTC_353|Compound:1988-1175|FD-Score:-5.37|P-value:4.69E-8 ID:SGTC_566|Compound:0958-0068|FD-Score:3.18|P-value:7.66E-4 ID:SGTC_600|Compound:1495-0418|FD-Score:-3.61|P-value:1.69E-4 ID:SGTC_752|Compound:1896-0090|FD-Score:5.44|P-value:2.99E-8 ID:SGTC_760|Compound:0568-0725|FD-Score:3.37|P-value:3.85E-4 ID:SGTC_772|Compound:k072-0172|FD-Score:-4.17|P-value:1.66E-5 ID:SGTC_815|Compound:1582-0027|FD-Score:3.19|P-value:7.38E-4 ID:SGTC_929|Compound:2805-0021|FD-Score:3.27|P-value:5.55E-4 ID:SGTC_960|Compound:1222-0186|FD-Score:5.17|P-value:1.33E-7 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.12|P-value:9.54E-4 ID:SGTC_1234|Compound:0326-0494|FD-Score:3.47|P-value:2.74E-4 ID:SGTC_1321|Compound:1326-2144|FD-Score:4.39|P-value:5.95E-6 ID:SGTC_1342|Compound:1486-1464|FD-Score:3.15|P-value:8.39E-4 ID:SGTC_1523|Compound:st012844|FD-Score:-5.16|P-value:1.41E-7 ID:SGTC_1527|Compound:coumarin|FD-Score:-3.85|P-value:6.51E-5 ID:SGTC_1695|Compound:st026914|FD-Score:3.11|P-value:9.66E-4 ID:SGTC_1724|Compound:st037281|FD-Score:3.67|P-value:1.28E-4 ID:SGTC_1794|Compound:st048918|FD-Score:-3.65|P-value:1.44E-4 ID:SGTC_1799|Compound:st045822|FD-Score:-3.43|P-value:3.28E-4 ID:SGTC_1807|Compound:st050092|FD-Score:3.44|P-value:3.01E-4 ID:SGTC_1809|Compound:st050081|FD-Score:-3.88|P-value:5.62E-5 ID:SGTC_1812|Compound:st052057|FD-Score:-3.55|P-value:2.05E-4 ID:SGTC_1820|Compound:st053765|FD-Score:-3.99|P-value:3.58E-5 ID:SGTC_2003|Compound:4023581|FD-Score:-4.59|P-value:2.48E-6 ID:SGTC_2008|Compound:4012253|FD-Score:-3.33|P-value:4.67E-4 ID:SGTC_2334|Compound:9017922|FD-Score:3.37|P-value:3.88E-4 ID:SGTC_2343|Compound:7990716|FD-Score:5.13|P-value:1.60E-7 ID:SGTC_2353|Compound:9059630|FD-Score:4.02|P-value:3.09E-5 ID:SGTC_2693|Compound:st077581|FD-Score:-6.32|P-value:1.64E-10 ID:SGTC_2695|Compound:st077686|FD-Score:4.10|P-value:2.21E-5 ID:SGTC_2795|Compound:7780010|FD-Score:5.17|P-value:1.31E-7 ID:SGTC_2844|Compound:9010103|FD-Score:3.52|P-value:2.25E-4 ID:SGTC_2877|Compound:9040724|FD-Score:3.37|P-value:3.90E-4 ID:SGTC_2976|Compound:9009005|FD-Score:-3.41|P-value:3.51E-4 ID:SGTC_2997|Compound:9067075|FD-Score:-3.55|P-value:2.07E-4 ID:SGTC_3144|Compound:9087057|FD-Score:-3.93|P-value:4.63E-5 ID:SGTC_35|Compound:0133-0054|FD-Score:-5.01|P-value:3.21E-7 ID:SGTC_42|Compound:0133-0086|FD-Score:-5.14|P-value:1.60E-7 ID:SGTC_335|Compound:4077-0256|FD-Score:-3.80|P-value:8.03E-5 ID:SGTC_353|Compound:1988-1175|FD-Score:-5.37|P-value:4.69E-8 ID:SGTC_566|Compound:0958-0068|FD-Score:3.18|P-value:7.66E-4 ID:SGTC_600|Compound:1495-0418|FD-Score:-3.61|P-value:1.69E-4 ID:SGTC_752|Compound:1896-0090|FD-Score:5.44|P-value:2.99E-8 ID:SGTC_760|Compound:0568-0725|FD-Score:3.37|P-value:3.85E-4 ID:SGTC_772|Compound:k072-0172|FD-Score:-4.17|P-value:1.66E-5 ID:SGTC_815|Compound:1582-0027|FD-Score:3.19|P-value:7.38E-4 ID:SGTC_929|Compound:2805-0021|FD-Score:3.27|P-value:5.55E-4 ID:SGTC_960|Compound:1222-0186|FD-Score:5.17|P-value:1.33E-7 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.12|P-value:9.54E-4 ID:SGTC_1234|Compound:0326-0494|FD-Score:3.47|P-value:2.74E-4 ID:SGTC_1321|Compound:1326-2144|FD-Score:4.39|P-value:5.95E-6 ID:SGTC_1342|Compound:1486-1464|FD-Score:3.15|P-value:8.39E-4 ID:SGTC_1523|Compound:st012844|FD-Score:-5.16|P-value:1.41E-7 ID:SGTC_1527|Compound:coumarin|FD-Score:-3.85|P-value:6.51E-5 ID:SGTC_1695|Compound:st026914|FD-Score:3.11|P-value:9.66E-4 ID:SGTC_1724|Compound:st037281|FD-Score:3.67|P-value:1.28E-4 ID:SGTC_1794|Compound:st048918|FD-Score:-3.65|P-value:1.44E-4 ID:SGTC_1799|Compound:st045822|FD-Score:-3.43|P-value:3.28E-4 ID:SGTC_1807|Compound:st050092|FD-Score:3.44|P-value:3.01E-4 ID:SGTC_1809|Compound:st050081|FD-Score:-3.88|P-value:5.62E-5 ID:SGTC_1812|Compound:st052057|FD-Score:-3.55|P-value:2.05E-4 ID:SGTC_1820|Compound:st053765|FD-Score:-3.99|P-value:3.58E-5 ID:SGTC_2003|Compound:4023581|FD-Score:-4.59|P-value:2.48E-6 ID:SGTC_2008|Compound:4012253|FD-Score:-3.33|P-value:4.67E-4 ID:SGTC_2334|Compound:9017922|FD-Score:3.37|P-value:3.88E-4 ID:SGTC_2343|Compound:7990716|FD-Score:5.13|P-value:1.60E-7 ID:SGTC_2353|Compound:9059630|FD-Score:4.02|P-value:3.09E-5 ID:SGTC_2693|Compound:st077581|FD-Score:-6.32|P-value:1.64E-10 ID:SGTC_2695|Compound:st077686|FD-Score:4.10|P-value:2.21E-5 ID:SGTC_2795|Compound:7780010|FD-Score:5.17|P-value:1.31E-7 ID:SGTC_2844|Compound:9010103|FD-Score:3.52|P-value:2.25E-4 ID:SGTC_2877|Compound:9040724|FD-Score:3.37|P-value:3.90E-4 ID:SGTC_2976|Compound:9009005|FD-Score:-3.41|P-value:3.51E-4 ID:SGTC_2997|Compound:9067075|FD-Score:-3.55|P-value:2.07E-4 ID:SGTC_3144|Compound:9087057|FD-Score:-3.93|P-value:4.63E-5

Top fitness defect scores for YNL188W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_752 1896-0090 5.44 130.0 µM 2.99E-8 TSC3-RPN4 1 2.67E-8 1.22
2 SGTC_2795 7780010 5.17 54.5 µM 1.31E-7 2 1.18E-7 0.18
3 SGTC_960 1222-0186 5.17 7.7 µM 1.33E-7 3 1.20E-7 0.01
4 SGTC_2343 7990716 5.13 14.8 µM 1.60E-7 1 1.45E-7 0.71
5 SGTC_1321 1326-2144 4.39 215.0 µM 5.95E-6 2 5.54E-6 0.42
6 SGTC_2695 st077686 4.10 52.0 µM 2.21E-5 2 2.08E-5 0.01
7 SGTC_2353 9059630 4.02 146.7 µM 3.09E-5 2 2.91E-5 0.81
8 SGTC_1724 st037281 3.67 8.3 µM 1.28E-4 19 1.22E-4 0.03
9 SGTC_2844 9010103 3.52 26.0 µM 2.25E-4 11 2.16E-4 0.00
10 SGTC_1234 0326-0494 3.47 15.5 µM 2.74E-4 6 2.63E-4 0.03
11 SGTC_1807 st050092 3.44 58.9 µM 3.01E-4 60S ribosome export 9 2.89E-4 0.01
12 SGTC_760 0568-0725 3.37 82.9 µM 3.85E-4 5 3.70E-4 0.18
13 SGTC_2334 9017922 3.37 200.0 µM 3.88E-4 6 3.73E-4 0.03
14 SGTC_2877 9040724 3.37 71.4 µM 3.90E-4 5 3.75E-4 0.27
15 SGTC_929 2805-0021 3.27 398.0 µM 5.55E-4 10 5.35E-4 0.17
16 SGTC_815 1582-0027 3.19 36.9 µM 7.38E-4 13 7.13E-4 0.07
17 SGTC_566 0958-0068 3.18 16.5 µM 7.66E-4 RSC & ERG11 28 7.41E-4 0.03
18 SGTC_1342 1486-1464 3.15 234.0 µM 8.39E-4 8 8.12E-4 0.00
19 SGTC_1695 st026914 3.11 34.5 µM 9.66E-4 9 9.35E-4 0.04
20 SGTC_1699 st031318 3.10 29.1 µM 0.00100 6 9.70E-4 0.07

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.133 9.94E-15 YGR025W_d YGR025W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.120 2.88E-12 YDR489W SLD5 het Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
0.110 1.72E-10 YDR094W_d YDR094W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2
0.104 1.53E-9 YMR239C RNT1 het Nuclear dsRNA-specific ribonuclease (RNase III); involved in rDNA transcription, rRNA processing and U2 snRNA 3' end formation by cleavage of a stem-loop structure at the 3' end of U2 snRNA; involved in polyadenylation-independent transcription termination; involved in the cell wall stress response, regulating the degradation of cell wall integrity and morphogenesis checkpoint genes
0.103 2.42E-9 YJR067C YAE1 het Protein that forms a complex with Lto1p and Rli1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species
0.102 2.73E-9 YGR245C SDA1 het Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis
0.096 2.63E-8 YGR097W ASK10 hom Component of RNA polymerase II holoenzyme; phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p; proposed to function in activation of the glycerol channel Fps1p; ASK10 has a paralog, RGC1, that arose from the whole genome duplication
0.094 5.59E-8 YGR169C PUS6 hom tRNA:pseudouridine synthase, catalyzes the conversion of uridine to pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability
0.092 9.54E-8 YFR057W_p YFR057W_p hom Putative protein of unknown function
0.089 2.29E-7 YGR246C BRF1 het TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB
0.089 2.68E-7 YJL090C DPB11 het DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress
0.088 2.97E-7 YPL177C CUP9 hom Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.088 3.82E-7 YBL030C PET9 het Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication
0.088 3.81E-7 YHR138C YHR138C hom Protein of unknown function; similar to Pbi2p; double null mutant lacking Pbi2p and Yhr138cp exhibits highly fragmented vacuoles; protein abundance increases in response to DNA replication stress
0.088 3.85E-7 YKL122C SRP21 het Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm