YNL189W / SRP1

Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation

Zygosity: Heterozygous strain
fixedexpanded
Profile for YNL189W / SRP1

Click on Significant Values for Screen Details ID:SGTC_55|Compound:0767-0285|FD-Score:3.29|P-value:7.22E-4 ID:SGTC_71|Compound:0417-1750|FD-Score:3.62|P-value:2.27E-4 ID:SGTC_75|Compound:0159-0059|FD-Score:-3.40|P-value:4.96E-4 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:-4.56|P-value:4.88E-6 ID:SGTC_122|Compound:0236-0016|FD-Score:5.37|P-value:1.01E-7 ID:SGTC_150|Compound:3970-0969|FD-Score:3.57|P-value:2.74E-4 ID:SGTC_214|Compound:4373-3496|FD-Score:3.56|P-value:2.92E-4 ID:SGTC_257|Compound:daunorubicin|FD-Score:-3.73|P-value:1.50E-4 ID:SGTC_375|Compound:3970-0961|FD-Score:3.20|P-value:9.84E-4 ID:SGTC_376|Compound:3970-0862|FD-Score:3.46|P-value:4.09E-4 ID:SGTC_377|Compound:3970-0964|FD-Score:7.84|P-value:1.61E-14 ID:SGTC_398|Compound:bronopol|FD-Score:3.73|P-value:1.51E-4 ID:SGTC_634|Compound:1611-4317|FD-Score:3.26|P-value:7.96E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:4.68|P-value:2.94E-6 ID:SGTC_997|Compound:3996-0078|FD-Score:3.76|P-value:1.38E-4 ID:SGTC_1003|Compound:4049-0218|FD-Score:-4.23|P-value:2.09E-5 ID:SGTC_1004|Compound:4084-0029|FD-Score:3.23|P-value:8.93E-4 ID:SGTC_1058|Compound:3448-5425|FD-Score:3.89|P-value:8.44E-5 ID:SGTC_1130|Compound:3454-2858|FD-Score:3.29|P-value:7.19E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:-3.33|P-value:6.29E-4 ID:SGTC_1260|Compound:0700-1617|FD-Score:4.25|P-value:1.95E-5 ID:SGTC_1261|Compound:0712-0010|FD-Score:5.31|P-value:1.39E-7 ID:SGTC_1262|Compound:0737-0114|FD-Score:-4.12|P-value:3.30E-5 ID:SGTC_1278|Compound:0874-0585|FD-Score:4.36|P-value:1.23E-5 ID:SGTC_1403|Compound:3909-7815|FD-Score:3.75|P-value:1.44E-4 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:-3.45|P-value:4.14E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:-3.55|P-value:2.89E-4 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:5.36|P-value:1.09E-7 ID:SGTC_1606|Compound:st000094|FD-Score:3.71|P-value:1.64E-4 ID:SGTC_1668|Compound:st014914|FD-Score:3.23|P-value:8.81E-4 ID:SGTC_1713|Compound:st033146|FD-Score:4.37|P-value:1.17E-5 ID:SGTC_1717|Compound:st033143|FD-Score:-3.80|P-value:1.16E-4 ID:SGTC_1724|Compound:st037281|FD-Score:4.19|P-value:2.53E-5 ID:SGTC_1880|Compound:MG-132|FD-Score:4.55|P-value:5.47E-6 ID:SGTC_1939|Compound:st074455|FD-Score:-4.15|P-value:2.93E-5 ID:SGTC_2016|Compound:5121758|FD-Score:-3.63|P-value:2.21E-4 ID:SGTC_2077|Compound:5212015|FD-Score:5.29|P-value:1.52E-7 ID:SGTC_2078|Compound:5212016|FD-Score:5.37|P-value:1.01E-7 ID:SGTC_2081|Compound:5214970|FD-Score:3.43|P-value:4.53E-4 ID:SGTC_2125|Compound:5246583|FD-Score:-3.51|P-value:3.35E-4 ID:SGTC_2147|Compound:5545932|FD-Score:3.27|P-value:7.75E-4 ID:SGTC_2163|Compound:5671233|FD-Score:5.40|P-value:8.71E-8 ID:SGTC_2347|Compound:9049783|FD-Score:-3.81|P-value:1.12E-4 ID:SGTC_2348|Compound:9022543|FD-Score:-6.57|P-value:9.84E-11 ID:SGTC_2358|Compound:9037157|FD-Score:4.69|P-value:2.81E-6 ID:SGTC_2396|Compound:5342950|FD-Score:-6.40|P-value:2.81E-10 ID:SGTC_2398|Compound:5228213|FD-Score:4.81|P-value:1.63E-6 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-3.30|P-value:6.78E-4 ID:SGTC_2473|Compound:5854593|FD-Score:5.53|P-value:4.42E-8 ID:SGTC_2504|Compound:purpurogallin|FD-Score:3.54|P-value:3.03E-4 ID:SGTC_2517|Compound:totarol|FD-Score:-3.22|P-value:8.97E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:3.80|P-value:1.17E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:4.32|P-value:1.49E-5 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:3.40|P-value:5.07E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:4.46|P-value:8.06E-6 ID:SGTC_2970|Compound:9090938|FD-Score:3.59|P-value:2.55E-4 ID:SGTC_3012|Compound:9080433|FD-Score:3.42|P-value:4.74E-4 ID:SGTC_3149|Compound:9097855|FD-Score:3.72|P-value:1.61E-4 ID:SGTC_3319|Compound:9138450|FD-Score:3.48|P-value:3.85E-4 ID:SGTC_3331|Compound:9141060|FD-Score:-3.30|P-value:6.94E-4 ID:SGTC_55|Compound:0767-0285|FD-Score:3.29|P-value:7.22E-4 ID:SGTC_71|Compound:0417-1750|FD-Score:3.62|P-value:2.27E-4 ID:SGTC_75|Compound:0159-0059|FD-Score:-3.40|P-value:4.96E-4 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:-4.56|P-value:4.88E-6 ID:SGTC_122|Compound:0236-0016|FD-Score:5.37|P-value:1.01E-7 ID:SGTC_150|Compound:3970-0969|FD-Score:3.57|P-value:2.74E-4 ID:SGTC_214|Compound:4373-3496|FD-Score:3.56|P-value:2.92E-4 ID:SGTC_257|Compound:daunorubicin|FD-Score:-3.73|P-value:1.50E-4 ID:SGTC_375|Compound:3970-0961|FD-Score:3.20|P-value:9.84E-4 ID:SGTC_376|Compound:3970-0862|FD-Score:3.46|P-value:4.09E-4 ID:SGTC_377|Compound:3970-0964|FD-Score:7.84|P-value:1.61E-14 ID:SGTC_398|Compound:bronopol|FD-Score:3.73|P-value:1.51E-4 ID:SGTC_634|Compound:1611-4317|FD-Score:3.26|P-value:7.96E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:4.68|P-value:2.94E-6 ID:SGTC_997|Compound:3996-0078|FD-Score:3.76|P-value:1.38E-4 ID:SGTC_1003|Compound:4049-0218|FD-Score:-4.23|P-value:2.09E-5 ID:SGTC_1004|Compound:4084-0029|FD-Score:3.23|P-value:8.93E-4 ID:SGTC_1058|Compound:3448-5425|FD-Score:3.89|P-value:8.44E-5 ID:SGTC_1130|Compound:3454-2858|FD-Score:3.29|P-value:7.19E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:-3.33|P-value:6.29E-4 ID:SGTC_1260|Compound:0700-1617|FD-Score:4.25|P-value:1.95E-5 ID:SGTC_1261|Compound:0712-0010|FD-Score:5.31|P-value:1.39E-7 ID:SGTC_1262|Compound:0737-0114|FD-Score:-4.12|P-value:3.30E-5 ID:SGTC_1278|Compound:0874-0585|FD-Score:4.36|P-value:1.23E-5 ID:SGTC_1403|Compound:3909-7815|FD-Score:3.75|P-value:1.44E-4 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:-3.45|P-value:4.14E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:-3.55|P-value:2.89E-4 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:5.36|P-value:1.09E-7 ID:SGTC_1606|Compound:st000094|FD-Score:3.71|P-value:1.64E-4 ID:SGTC_1668|Compound:st014914|FD-Score:3.23|P-value:8.81E-4 ID:SGTC_1713|Compound:st033146|FD-Score:4.37|P-value:1.17E-5 ID:SGTC_1717|Compound:st033143|FD-Score:-3.80|P-value:1.16E-4 ID:SGTC_1724|Compound:st037281|FD-Score:4.19|P-value:2.53E-5 ID:SGTC_1880|Compound:MG-132|FD-Score:4.55|P-value:5.47E-6 ID:SGTC_1939|Compound:st074455|FD-Score:-4.15|P-value:2.93E-5 ID:SGTC_2016|Compound:5121758|FD-Score:-3.63|P-value:2.21E-4 ID:SGTC_2077|Compound:5212015|FD-Score:5.29|P-value:1.52E-7 ID:SGTC_2078|Compound:5212016|FD-Score:5.37|P-value:1.01E-7 ID:SGTC_2081|Compound:5214970|FD-Score:3.43|P-value:4.53E-4 ID:SGTC_2125|Compound:5246583|FD-Score:-3.51|P-value:3.35E-4 ID:SGTC_2147|Compound:5545932|FD-Score:3.27|P-value:7.75E-4 ID:SGTC_2163|Compound:5671233|FD-Score:5.40|P-value:8.71E-8 ID:SGTC_2347|Compound:9049783|FD-Score:-3.81|P-value:1.12E-4 ID:SGTC_2348|Compound:9022543|FD-Score:-6.57|P-value:9.84E-11 ID:SGTC_2358|Compound:9037157|FD-Score:4.69|P-value:2.81E-6 ID:SGTC_2396|Compound:5342950|FD-Score:-6.40|P-value:2.81E-10 ID:SGTC_2398|Compound:5228213|FD-Score:4.81|P-value:1.63E-6 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-3.30|P-value:6.78E-4 ID:SGTC_2473|Compound:5854593|FD-Score:5.53|P-value:4.42E-8 ID:SGTC_2504|Compound:purpurogallin|FD-Score:3.54|P-value:3.03E-4 ID:SGTC_2517|Compound:totarol|FD-Score:-3.22|P-value:8.97E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:3.80|P-value:1.17E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:4.32|P-value:1.49E-5 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:3.40|P-value:5.07E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:4.46|P-value:8.06E-6 ID:SGTC_2970|Compound:9090938|FD-Score:3.59|P-value:2.55E-4 ID:SGTC_3012|Compound:9080433|FD-Score:3.42|P-value:4.74E-4 ID:SGTC_3149|Compound:9097855|FD-Score:3.72|P-value:1.61E-4 ID:SGTC_3319|Compound:9138450|FD-Score:3.48|P-value:3.85E-4 ID:SGTC_3331|Compound:9141060|FD-Score:-3.30|P-value:6.94E-4

Top fitness defect scores for YNL189W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_377 3970-0964 7.84 33.1 µM 1.61E-14 1 2.23E-15 1.34
2 SGTC_2473 5854593 5.53 8.9 µM 4.42E-8 1 1.62E-8 1.00
3 SGTC_2163 5671233 5.40 125.0 µM 8.71E-8 5 3.34E-8 0.09
4 SGTC_122 0236-0016 5.37 402.4 µM 1.01E-7 RNA processing & uracil transport 5 3.90E-8 0.10
5 SGTC_2078 5212016 5.37 200.0 µM 1.01E-7 60S ribosome export 4 3.90E-8 0.86
6 SGTC_1595 6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin 5.36 67.5 µM 1.09E-7 4 4.23E-8 0.02
7 SGTC_1261 0712-0010 5.31 12.7 µM 1.39E-7 RNA processing & uracil transport 4 5.50E-8 0.54
8 SGTC_2077 5212015 5.29 200.0 µM 1.52E-7 3 6.05E-8 0.11
9 SGTC_2398 5228213 4.81 48.8 µM 1.63E-6 iron homeostasis 10 7.58E-7 0.00
10 SGTC_2358 9037157 4.69 4.0 µM 2.81E-6 10 1.35E-6 0.52
11 SGTC_852 0418-0143 4.68 439.0 µM 2.94E-6 calcium & mitochondrial duress 7 1.41E-6 0.23
12 SGTC_1880 MG-132 4.55 525.0 µM 5.47E-6 17 2.74E-6 0.36
13 SGTC_2636 apigenin dimethyl ether 4.46 100.0 µM 8.06E-6 9 4.14E-6 0.11
14 SGTC_1713 st033146 4.37 12.8 µM 1.17E-5 6 6.18E-6 0.26
15 SGTC_1278 0874-0585 4.36 172.0 µM 1.23E-5 2 6.51E-6 0.50
16 SGTC_2593 nobiletin 4.32 86.2 µM 1.49E-5 9 7.98E-6 0.20
17 SGTC_1260 0700-1617 4.25 7.8 µM 1.95E-5 calcium & mitochondrial duress 3 1.06E-5 1.75E-4
18 SGTC_1724 st037281 4.19 8.3 µM 2.53E-5 12 1.40E-5 0.10
19 SGTC_1058 3448-5425 3.89 97.3 µM 8.44E-5 10 5.04E-5 0.18
20 SGTC_2533 agelasine 3.80 13.7 µM 1.17E-4 ERG2 15 7.17E-5 0.11

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.104 1.57E-9 YLR347C KAP95 het Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p
0.095 3.56E-8 YBR258C SHG1 hom Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres
0.093 6.48E-8 YHR086W NAM8 hom RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function
0.093 6.50E-8 YJR042W NUP85 het Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75
0.089 2.87E-7 YJL101C GSH1 hom Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress
0.087 4.06E-7 YOR016C ERP4 hom Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport
0.087 5.09E-7 YNL209W SSB2 hom Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in the folding of newly-synthesized polypeptide chains; member of the HSP70 family; SSB2 has a paralog, SSB1, that arose from the whole genome duplication
0.085 8.05E-7 YEL055C POL5 het DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA
0.083 1.55E-6 YFL025C BST1 hom GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules
0.082 1.88E-6 YHR049W FSH1 hom Putative serine hydrolase that localizes to both the nucleus and cytoplasm; sequence is similar to S. cerevisiae Fsh2p and Fsh3p and the human candidate tumor suppressor OVCA2
0.080 3.64E-6 YDR432W NPL3 hom RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress
0.080 3.73E-6 YMR092C AIP1 hom Actin cortical patch component; interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats; protein increases in abundance and relocalizes from cytoplasm to plasma membrane upon DNA replication stress
0.078 5.56E-6 YLR119W SRN2 hom Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus
0.076 1.08E-5 YGR167W CLC1 hom Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W
0.075 1.31E-5 YER062C HOR2 hom Glycerol-1-phosphatase involved in glycerol biosynthesis; induced in response to hyperosmotic or oxidative stress, and during diauxic shift; HOR2 has a paralog, RHR2, that arose from the whole genome duplication