YNL197C / WHI3

RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci;; Tpk1 (PKA) mediated phosphorylation of Whi3 affects G1/S transition by modulating Cln3 transcription; cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start; WHI3 has a paralog, WHI4, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL197C / WHI3

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:5.93|P-value:3.46E-9 ID:SGTC_62|Compound:0180-0423|FD-Score:3.26|P-value:7.25E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:5.03|P-value:4.38E-7 ID:SGTC_66|Compound:0109-0187|FD-Score:4.12|P-value:2.80E-5 ID:SGTC_74|Compound:000s-0487|FD-Score:3.43|P-value:4.02E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:3.35|P-value:5.29E-4 ID:SGTC_167|Compound:3672-0051|FD-Score:3.76|P-value:1.16E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:4.94|P-value:7.00E-7 ID:SGTC_207|Compound:4185-0010|FD-Score:4.36|P-value:1.03E-5 ID:SGTC_266|Compound:formosanin C|FD-Score:3.51|P-value:2.99E-4 ID:SGTC_316|Compound:k060-0003|FD-Score:3.37|P-value:4.94E-4 ID:SGTC_355|Compound:1311-2252|FD-Score:3.77|P-value:1.12E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:5.71|P-value:1.22E-8 ID:SGTC_528|Compound:3492-0019|FD-Score:4.42|P-value:7.71E-6 ID:SGTC_598|Compound:3937-0236|FD-Score:3.22|P-value:8.11E-4 ID:SGTC_599|Compound:1935-0052|FD-Score:3.18|P-value:9.33E-4 ID:SGTC_611|Compound:3453-1652|FD-Score:-4.52|P-value:5.36E-6 ID:SGTC_733|Compound:4069-0007|FD-Score:3.17|P-value:9.71E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:3.43|P-value:3.95E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:3.16|P-value:9.99E-4 ID:SGTC_1005|Compound:4092-0690|FD-Score:3.77|P-value:1.12E-4 ID:SGTC_1014|Compound:4130-3005|FD-Score:3.21|P-value:8.58E-4 ID:SGTC_1041|Compound:0206-0046|FD-Score:4.96|P-value:6.26E-7 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.47|P-value:3.45E-4 ID:SGTC_1045|Compound:1348-1485|FD-Score:3.16|P-value:9.93E-4 ID:SGTC_1147|Compound:3809-1045|FD-Score:5.92|P-value:3.66E-9 ID:SGTC_1154|Compound:4088-0362|FD-Score:5.13|P-value:2.73E-7 ID:SGTC_1155|Compound:4088-0394|FD-Score:3.70|P-value:1.49E-4 ID:SGTC_1156|Compound:4092-0367|FD-Score:3.50|P-value:3.11E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:-3.47|P-value:3.64E-4 ID:SGTC_1315|Compound:1237-0019|FD-Score:3.24|P-value:7.68E-4 ID:SGTC_1393|Compound:3470-5649|FD-Score:-4.30|P-value:1.41E-5 ID:SGTC_1428|Compound:4049-0204|FD-Score:3.41|P-value:4.28E-4 ID:SGTC_1447|Compound:4239-0816|FD-Score:3.87|P-value:7.63E-5 ID:SGTC_1523|Compound:st012844|FD-Score:4.25|P-value:1.66E-5 ID:SGTC_1609|Compound:st001574|FD-Score:3.62|P-value:1.99E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:4.05|P-value:3.72E-5 ID:SGTC_1745|Compound:st081495|FD-Score:-4.03|P-value:4.30E-5 ID:SGTC_1749|Compound:st037932|FD-Score:3.56|P-value:2.50E-4 ID:SGTC_1750|Compound:st081859|FD-Score:6.07|P-value:1.53E-9 ID:SGTC_1788|Compound:fluconazole|FD-Score:5.49|P-value:4.05E-8 ID:SGTC_1822|Compound:st052468|FD-Score:3.16|P-value:9.93E-4 ID:SGTC_1824|Compound:st053723|FD-Score:3.52|P-value:2.92E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.69|P-value:2.23E-6 ID:SGTC_2088|Compound:5278280|FD-Score:3.57|P-value:2.42E-4 ID:SGTC_2125|Compound:5246583|FD-Score:-3.25|P-value:7.72E-4 ID:SGTC_2322|Compound:7413319|FD-Score:3.94|P-value:5.79E-5 ID:SGTC_2340|Compound:7987427|FD-Score:4.10|P-value:3.07E-5 ID:SGTC_2351|Compound:9026858|FD-Score:-3.29|P-value:6.71E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:3.97|P-value:5.10E-5 ID:SGTC_2496|Compound:diffractaic acid|FD-Score:3.31|P-value:6.06E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:3.88|P-value:7.42E-5 ID:SGTC_2544|Compound:sphondin|FD-Score:3.24|P-value:7.57E-4 ID:SGTC_2549|Compound:cedrol|FD-Score:3.46|P-value:3.52E-4 ID:SGTC_2576|Compound:guaiol|FD-Score:9.14|P-value:2.22E-19 ID:SGTC_2679|Compound:rapamycin|FD-Score:4.13|P-value:2.64E-5 ID:SGTC_2778|Compound:tioconazole|FD-Score:3.37|P-value:4.88E-4 ID:SGTC_2790|Compound:5423970|FD-Score:3.60|P-value:2.14E-4 ID:SGTC_2798|Compound:7813637|FD-Score:4.08|P-value:3.25E-5 ID:SGTC_2836|Compound:9010338|FD-Score:3.95|P-value:5.65E-5 ID:SGTC_2912|Compound:7964298|FD-Score:3.27|P-value:6.99E-4 ID:SGTC_2969|Compound:9091408|FD-Score:3.37|P-value:4.95E-4 ID:SGTC_2972|Compound:9093619|FD-Score:3.53|P-value:2.76E-4 ID:SGTC_2997|Compound:9067075|FD-Score:5.24|P-value:1.53E-7 ID:SGTC_3011|Compound:9081274|FD-Score:4.33|P-value:1.17E-5 ID:SGTC_3015|Compound:9080845|FD-Score:-3.37|P-value:5.13E-4 ID:SGTC_3037|Compound:9091026|FD-Score:-3.21|P-value:8.80E-4 ID:SGTC_3093|Compound:9117344|FD-Score:4.12|P-value:2.87E-5 ID:SGTC_3197|Compound:9113331|FD-Score:3.24|P-value:7.68E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:5.93|P-value:3.46E-9 ID:SGTC_62|Compound:0180-0423|FD-Score:3.26|P-value:7.25E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:5.03|P-value:4.38E-7 ID:SGTC_66|Compound:0109-0187|FD-Score:4.12|P-value:2.80E-5 ID:SGTC_74|Compound:000s-0487|FD-Score:3.43|P-value:4.02E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:3.35|P-value:5.29E-4 ID:SGTC_167|Compound:3672-0051|FD-Score:3.76|P-value:1.16E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:4.94|P-value:7.00E-7 ID:SGTC_207|Compound:4185-0010|FD-Score:4.36|P-value:1.03E-5 ID:SGTC_266|Compound:formosanin C|FD-Score:3.51|P-value:2.99E-4 ID:SGTC_316|Compound:k060-0003|FD-Score:3.37|P-value:4.94E-4 ID:SGTC_355|Compound:1311-2252|FD-Score:3.77|P-value:1.12E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:5.71|P-value:1.22E-8 ID:SGTC_528|Compound:3492-0019|FD-Score:4.42|P-value:7.71E-6 ID:SGTC_598|Compound:3937-0236|FD-Score:3.22|P-value:8.11E-4 ID:SGTC_599|Compound:1935-0052|FD-Score:3.18|P-value:9.33E-4 ID:SGTC_611|Compound:3453-1652|FD-Score:-4.52|P-value:5.36E-6 ID:SGTC_733|Compound:4069-0007|FD-Score:3.17|P-value:9.71E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:3.43|P-value:3.95E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:3.16|P-value:9.99E-4 ID:SGTC_1005|Compound:4092-0690|FD-Score:3.77|P-value:1.12E-4 ID:SGTC_1014|Compound:4130-3005|FD-Score:3.21|P-value:8.58E-4 ID:SGTC_1041|Compound:0206-0046|FD-Score:4.96|P-value:6.26E-7 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.47|P-value:3.45E-4 ID:SGTC_1045|Compound:1348-1485|FD-Score:3.16|P-value:9.93E-4 ID:SGTC_1147|Compound:3809-1045|FD-Score:5.92|P-value:3.66E-9 ID:SGTC_1154|Compound:4088-0362|FD-Score:5.13|P-value:2.73E-7 ID:SGTC_1155|Compound:4088-0394|FD-Score:3.70|P-value:1.49E-4 ID:SGTC_1156|Compound:4092-0367|FD-Score:3.50|P-value:3.11E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:-3.47|P-value:3.64E-4 ID:SGTC_1315|Compound:1237-0019|FD-Score:3.24|P-value:7.68E-4 ID:SGTC_1393|Compound:3470-5649|FD-Score:-4.30|P-value:1.41E-5 ID:SGTC_1428|Compound:4049-0204|FD-Score:3.41|P-value:4.28E-4 ID:SGTC_1447|Compound:4239-0816|FD-Score:3.87|P-value:7.63E-5 ID:SGTC_1523|Compound:st012844|FD-Score:4.25|P-value:1.66E-5 ID:SGTC_1609|Compound:st001574|FD-Score:3.62|P-value:1.99E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:4.05|P-value:3.72E-5 ID:SGTC_1745|Compound:st081495|FD-Score:-4.03|P-value:4.30E-5 ID:SGTC_1749|Compound:st037932|FD-Score:3.56|P-value:2.50E-4 ID:SGTC_1750|Compound:st081859|FD-Score:6.07|P-value:1.53E-9 ID:SGTC_1788|Compound:fluconazole|FD-Score:5.49|P-value:4.05E-8 ID:SGTC_1822|Compound:st052468|FD-Score:3.16|P-value:9.93E-4 ID:SGTC_1824|Compound:st053723|FD-Score:3.52|P-value:2.92E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.69|P-value:2.23E-6 ID:SGTC_2088|Compound:5278280|FD-Score:3.57|P-value:2.42E-4 ID:SGTC_2125|Compound:5246583|FD-Score:-3.25|P-value:7.72E-4 ID:SGTC_2322|Compound:7413319|FD-Score:3.94|P-value:5.79E-5 ID:SGTC_2340|Compound:7987427|FD-Score:4.10|P-value:3.07E-5 ID:SGTC_2351|Compound:9026858|FD-Score:-3.29|P-value:6.71E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:3.97|P-value:5.10E-5 ID:SGTC_2496|Compound:diffractaic acid|FD-Score:3.31|P-value:6.06E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:3.88|P-value:7.42E-5 ID:SGTC_2544|Compound:sphondin|FD-Score:3.24|P-value:7.57E-4 ID:SGTC_2549|Compound:cedrol|FD-Score:3.46|P-value:3.52E-4 ID:SGTC_2576|Compound:guaiol|FD-Score:9.14|P-value:2.22E-19 ID:SGTC_2679|Compound:rapamycin|FD-Score:4.13|P-value:2.64E-5 ID:SGTC_2778|Compound:tioconazole|FD-Score:3.37|P-value:4.88E-4 ID:SGTC_2790|Compound:5423970|FD-Score:3.60|P-value:2.14E-4 ID:SGTC_2798|Compound:7813637|FD-Score:4.08|P-value:3.25E-5 ID:SGTC_2836|Compound:9010338|FD-Score:3.95|P-value:5.65E-5 ID:SGTC_2912|Compound:7964298|FD-Score:3.27|P-value:6.99E-4 ID:SGTC_2969|Compound:9091408|FD-Score:3.37|P-value:4.95E-4 ID:SGTC_2972|Compound:9093619|FD-Score:3.53|P-value:2.76E-4 ID:SGTC_2997|Compound:9067075|FD-Score:5.24|P-value:1.53E-7 ID:SGTC_3011|Compound:9081274|FD-Score:4.33|P-value:1.17E-5 ID:SGTC_3015|Compound:9080845|FD-Score:-3.37|P-value:5.13E-4 ID:SGTC_3037|Compound:9091026|FD-Score:-3.21|P-value:8.80E-4 ID:SGTC_3093|Compound:9117344|FD-Score:4.12|P-value:2.87E-5 ID:SGTC_3197|Compound:9113331|FD-Score:3.24|P-value:7.68E-4

Top fitness defect scores for YNL197C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2576 guaiol 9.14 23.1 µM 2.22E-19 1 3.18E-20
2 SGTC_1750 st081859 6.07 2.4 µM 1.53E-9 endomembrane recycling 107 6.52E-10
3 SGTC_58 0957-0393 5.93 9.0 µM 3.46E-9 cell wall 217 1.53E-9
4 SGTC_1147 3809-1045 5.92 12.3 µM 3.66E-9 1 1.63E-9
5 SGTC_515 4092-1064 5.71 102.0 µM 1.22E-8 plasma membrane duress 26 5.72E-9
6 SGTC_1788 fluconazole 5.49 20.0 µM 4.05E-8 azole & statin 35 2.01E-8
7 SGTC_2997 9067075 5.24 71.4 µM 1.53E-7 unfolded protein response 26 8.07E-8
8 SGTC_1154 4088-0362 5.13 150.0 µM 2.73E-7 4 1.49E-7
9 SGTC_65 0798-0905 5.03 35.0 µM 4.38E-7 calcium & mitochondrial duress 52 2.44E-7
10 SGTC_1041 0206-0046 4.96 107.0 µM 6.26E-7 15 3.54E-7
11 SGTC_178 4092-0806 4.94 87.5 µM 7.00E-7 ergosterol depletion effects on membrane 58 3.98E-7
12 SGTC_1844 rapamycin 4.69 4.0 nM 2.23E-6 122 1.34E-6
13 SGTC_528 3492-0019 4.42 172.0 µM 7.71E-6 calcium & mitochondrial duress 185 4.90E-6
14 SGTC_207 4185-0010 4.36 4.5 µM 1.03E-5 endomembrane recycling 75 6.61E-6
15 SGTC_3011 9081274 4.33 71.4 µM 1.17E-5 8 7.58E-6
16 SGTC_1523 st012844 4.25 44.7 µM 1.66E-5 60S ribosome export 13 1.09E-5
17 SGTC_2679 rapamycin 4.13 1.0 nM 2.64E-5 130 1.78E-5
18 SGTC_66 0109-0187 4.12 42.4 µM 2.80E-5 33 1.88E-5
19 SGTC_3093 9117344 4.12 49.5 µM 2.87E-5 60S ribosome export 14 1.93E-5
20 SGTC_2340 7987427 4.10 183.4 µM 3.07E-5 28 2.08E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.153 4.08E-19 YPR029C APL4 hom Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport
0.139 5.49E-16 YHL019C APM2 hom Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport
0.135 4.60E-15 YDL160C DHH1 hom Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress
0.134 8.45E-15 YDR159W SAC3 hom Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP)
0.132 1.78E-14 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.131 3.16E-14 YHR036W BRL1 het Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
0.130 4.63E-14 YJR060W CBF1 hom Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress
0.130 4.94E-14 YJL187C SWE1 hom Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate
0.128 9.53E-14 YMR126C_p DLT1_p hom Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
0.127 1.70E-13 YGR217W CCH1 hom Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together
0.126 3.00E-13 YKL135C APL2 hom Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress
0.125 3.93E-13 YML007C-A_p YML007C-A_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria
0.123 8.89E-13 YIL052C RPL34B hom Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication
0.122 1.60E-12 YLL043W FPS1 hom Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress
0.119 4.11E-12 YCL005W LDB16 hom Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria