YNL233W / BNI4

Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL233W / BNI4

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:9.78|P-value:1.32E-19 ID:SGTC_65|Compound:0798-0905|FD-Score:4.92|P-value:3.71E-6 ID:SGTC_77|Compound:0828-0286|FD-Score:3.62|P-value:5.29E-4 ID:SGTC_129|Compound:0088-0017|FD-Score:-3.60|P-value:3.38E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:13.30|P-value:1.72E-34 ID:SGTC_222|Compound:nsc-310342|FD-Score:3.43|P-value:9.49E-4 ID:SGTC_271|Compound:rotenone|FD-Score:3.63|P-value:4.99E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:3.73|P-value:3.60E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:4.29|P-value:4.89E-5 ID:SGTC_586|Compound:0957-0265|FD-Score:8.79|P-value:3.77E-16 ID:SGTC_631|Compound:k072-0230|FD-Score:3.64|P-value:4.87E-4 ID:SGTC_652|Compound:0437-0092|FD-Score:3.59|P-value:5.73E-4 ID:SGTC_661|Compound:1304-0007|FD-Score:-3.47|P-value:5.19E-4 ID:SGTC_666|Compound:0272-0154|FD-Score:5.28|P-value:7.46E-7 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:3.43|P-value:9.51E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:6.28|P-value:4.72E-9 ID:SGTC_977|Compound:1348-1378|FD-Score:-4.89|P-value:2.20E-6 ID:SGTC_984|Compound:1492-0255|FD-Score:7.98|P-value:1.30E-13 ID:SGTC_1004|Compound:4084-0029|FD-Score:3.96|P-value:1.63E-4 ID:SGTC_1021|Compound:4476-0760|FD-Score:3.97|P-value:1.56E-4 ID:SGTC_1041|Compound:0206-0046|FD-Score:4.05|P-value:1.17E-4 ID:SGTC_1293|Compound:1000-1278|FD-Score:4.65|P-value:1.17E-5 ID:SGTC_1633|Compound:st005664|FD-Score:3.49|P-value:8.02E-4 ID:SGTC_1800|Compound:st050079|FD-Score:4.57|P-value:1.62E-5 ID:SGTC_1801|Compound:st050089|FD-Score:3.89|P-value:2.12E-4 ID:SGTC_1818|Compound:st052136|FD-Score:4.50|P-value:2.13E-5 ID:SGTC_1901|Compound:st060216|FD-Score:3.51|P-value:7.43E-4 ID:SGTC_1903|Compound:st060211|FD-Score:-3.27|P-value:9.91E-4 ID:SGTC_1910|Compound:st062352|FD-Score:3.72|P-value:3.74E-4 ID:SGTC_2048|Compound:5246248|FD-Score:-3.36|P-value:7.37E-4 ID:SGTC_2168|Compound:5727979|FD-Score:-3.80|P-value:1.69E-4 ID:SGTC_2171|Compound:5739187|FD-Score:-4.30|P-value:2.59E-5 ID:SGTC_2174|Compound:5790901|FD-Score:-4.18|P-value:4.06E-5 ID:SGTC_2210|Compound:7115885|FD-Score:4.31|P-value:4.44E-5 ID:SGTC_2289|Compound:7946404|FD-Score:4.32|P-value:4.35E-5 ID:SGTC_2322|Compound:7413319|FD-Score:4.79|P-value:6.59E-6 ID:SGTC_2324|Compound:6149169|FD-Score:4.59|P-value:1.50E-5 ID:SGTC_2326|Compound:6164455|FD-Score:-3.56|P-value:3.80E-4 ID:SGTC_2327|Compound:6189656|FD-Score:4.29|P-value:4.87E-5 ID:SGTC_2563|Compound:tomatine|FD-Score:4.88|P-value:4.42E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:6.14|P-value:9.85E-9 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.42|P-value:9.93E-4 ID:SGTC_2854|Compound:9024559|FD-Score:4.28|P-value:5.11E-5 ID:SGTC_2912|Compound:7964298|FD-Score:5.81|P-value:5.71E-8 ID:SGTC_2925|Compound:9010536|FD-Score:-3.68|P-value:2.53E-4 ID:SGTC_2976|Compound:9009005|FD-Score:-4.93|P-value:1.83E-6 ID:SGTC_2978|Compound:9011784|FD-Score:-5.60|P-value:7.51E-8 ID:SGTC_3153|Compound:9100258|FD-Score:3.71|P-value:3.90E-4 ID:SGTC_3228|Compound:9131799|FD-Score:3.57|P-value:6.03E-4 ID:SGTC_3259|Compound:9138485|FD-Score:3.88|P-value:2.13E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:9.78|P-value:1.32E-19 ID:SGTC_65|Compound:0798-0905|FD-Score:4.92|P-value:3.71E-6 ID:SGTC_77|Compound:0828-0286|FD-Score:3.62|P-value:5.29E-4 ID:SGTC_129|Compound:0088-0017|FD-Score:-3.60|P-value:3.38E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:13.30|P-value:1.72E-34 ID:SGTC_222|Compound:nsc-310342|FD-Score:3.43|P-value:9.49E-4 ID:SGTC_271|Compound:rotenone|FD-Score:3.63|P-value:4.99E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:3.73|P-value:3.60E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:4.29|P-value:4.89E-5 ID:SGTC_586|Compound:0957-0265|FD-Score:8.79|P-value:3.77E-16 ID:SGTC_631|Compound:k072-0230|FD-Score:3.64|P-value:4.87E-4 ID:SGTC_652|Compound:0437-0092|FD-Score:3.59|P-value:5.73E-4 ID:SGTC_661|Compound:1304-0007|FD-Score:-3.47|P-value:5.19E-4 ID:SGTC_666|Compound:0272-0154|FD-Score:5.28|P-value:7.46E-7 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:3.43|P-value:9.51E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:6.28|P-value:4.72E-9 ID:SGTC_977|Compound:1348-1378|FD-Score:-4.89|P-value:2.20E-6 ID:SGTC_984|Compound:1492-0255|FD-Score:7.98|P-value:1.30E-13 ID:SGTC_1004|Compound:4084-0029|FD-Score:3.96|P-value:1.63E-4 ID:SGTC_1021|Compound:4476-0760|FD-Score:3.97|P-value:1.56E-4 ID:SGTC_1041|Compound:0206-0046|FD-Score:4.05|P-value:1.17E-4 ID:SGTC_1293|Compound:1000-1278|FD-Score:4.65|P-value:1.17E-5 ID:SGTC_1633|Compound:st005664|FD-Score:3.49|P-value:8.02E-4 ID:SGTC_1800|Compound:st050079|FD-Score:4.57|P-value:1.62E-5 ID:SGTC_1801|Compound:st050089|FD-Score:3.89|P-value:2.12E-4 ID:SGTC_1818|Compound:st052136|FD-Score:4.50|P-value:2.13E-5 ID:SGTC_1901|Compound:st060216|FD-Score:3.51|P-value:7.43E-4 ID:SGTC_1903|Compound:st060211|FD-Score:-3.27|P-value:9.91E-4 ID:SGTC_1910|Compound:st062352|FD-Score:3.72|P-value:3.74E-4 ID:SGTC_2048|Compound:5246248|FD-Score:-3.36|P-value:7.37E-4 ID:SGTC_2168|Compound:5727979|FD-Score:-3.80|P-value:1.69E-4 ID:SGTC_2171|Compound:5739187|FD-Score:-4.30|P-value:2.59E-5 ID:SGTC_2174|Compound:5790901|FD-Score:-4.18|P-value:4.06E-5 ID:SGTC_2210|Compound:7115885|FD-Score:4.31|P-value:4.44E-5 ID:SGTC_2289|Compound:7946404|FD-Score:4.32|P-value:4.35E-5 ID:SGTC_2322|Compound:7413319|FD-Score:4.79|P-value:6.59E-6 ID:SGTC_2324|Compound:6149169|FD-Score:4.59|P-value:1.50E-5 ID:SGTC_2326|Compound:6164455|FD-Score:-3.56|P-value:3.80E-4 ID:SGTC_2327|Compound:6189656|FD-Score:4.29|P-value:4.87E-5 ID:SGTC_2563|Compound:tomatine|FD-Score:4.88|P-value:4.42E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:6.14|P-value:9.85E-9 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.42|P-value:9.93E-4 ID:SGTC_2854|Compound:9024559|FD-Score:4.28|P-value:5.11E-5 ID:SGTC_2912|Compound:7964298|FD-Score:5.81|P-value:5.71E-8 ID:SGTC_2925|Compound:9010536|FD-Score:-3.68|P-value:2.53E-4 ID:SGTC_2976|Compound:9009005|FD-Score:-4.93|P-value:1.83E-6 ID:SGTC_2978|Compound:9011784|FD-Score:-5.60|P-value:7.51E-8 ID:SGTC_3153|Compound:9100258|FD-Score:3.71|P-value:3.90E-4 ID:SGTC_3228|Compound:9131799|FD-Score:3.57|P-value:6.03E-4 ID:SGTC_3259|Compound:9138485|FD-Score:3.88|P-value:2.13E-4

Top fitness defect scores for YNL233W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_138 0850-0259 13.30 40.9 µM 1.72E-34 cell wall 22 2.18E-40
2 SGTC_58 0957-0393 9.78 9.0 µM 1.32E-19 cell wall 82 6.57E-23
3 SGTC_586 0957-0265 8.79 8.2 µM 3.77E-16 cell wall 5 7.56E-19
4 SGTC_984 1492-0255 7.98 2.9 µM 1.30E-13 SWF1 & branched chain AA biosynthesis 5 7.28E-16
5 SGTC_964 1287-0107 6.28 19.4 µM 4.72E-9 cell wall 10 1.69E-10
6 SGTC_2582 agaric acid 6.14 100.0 µM 9.85E-9 redox potentiating 151 4.02E-10
7 SGTC_2912 7964298 5.81 10.9 µM 5.71E-8 cell wall 50 3.18E-9
8 SGTC_666 0272-0154 5.28 136.0 µM 7.46E-7 cell wall 26 6.56E-8
9 SGTC_65 0798-0905 4.92 35.0 µM 3.71E-6 calcium & mitochondrial duress 55 4.34E-7
10 SGTC_2563 tomatine 4.88 8.5 µM 4.42E-6 22 5.33E-7
11 SGTC_2322 7413319 4.79 38.2 µM 6.59E-6 ERAD & cell cycle 108 8.54E-7
12 SGTC_1293 1000-1278 4.65 195.0 µM 1.17E-5 4 1.67E-6
13 SGTC_2324 6149169 4.59 200.0 µM 1.50E-5 15 2.25E-6
14 SGTC_1800 st050079 4.57 29.1 µM 1.62E-5 NEO1-PIK1 91 2.46E-6
15 SGTC_1818 st052136 4.50 56.3 µM 2.13E-5 NEO1-PIK1 30 3.40E-6
16 SGTC_2289 7946404 4.32 165.1 µM 4.35E-5 81 7.89E-6
17 SGTC_2210 7115885 4.31 190.4 µM 4.44E-5 cell wall signaling 27 8.09E-6
18 SGTC_2327 6189656 4.29 200.0 µM 4.87E-5 15 9.01E-6
19 SGTC_453 enantio-paf c16 4.29 10.0 µM 4.89E-5 121 9.06E-6
20 SGTC_2854 9024559 4.28 71.4 µM 5.11E-5 15 9.54E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.413 9.98E-139 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.351 9.17E-98 YLL043W FPS1 hom Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress
0.347 1.23E-95 YJL099W CHS6 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.323 1.76E-82 YBR023C CHS3 hom Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
0.299 1.65E-70 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.294 4.31E-68 YLR332W MID2 hom O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p
0.286 2.25E-64 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.286 3.93E-64 YBR091C TIM12 het Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins
0.284 2.57E-63 YKL076C_d PSY1_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
0.283 7.88E-63 YDR348C PAL1 hom Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.281 6.32E-62 YPL066W RGL1 hom Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
0.277 5.32E-60 YDR351W SBE2 hom Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth
0.271 2.27E-57 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.259 1.29E-52 YDR335W MSN5 hom Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm
0.256 2.94E-51 YPL115C BEM3 hom Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly