YNL236W / SIN4

Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL236W / SIN4

Click on Significant Values for Screen Details ID:SGTC_23|Compound:0213-0011|FD-Score:4.28|P-value:7.76E-5 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.97|P-value:1.57E-4 ID:SGTC_100|Compound:0086-0128|FD-Score:7.07|P-value:1.70E-10 ID:SGTC_124|Compound:0136-0217|FD-Score:4.46|P-value:4.01E-5 ID:SGTC_129|Compound:0088-0017|FD-Score:5.08|P-value:3.52E-6 ID:SGTC_134|Compound:0269-0018|FD-Score:14.50|P-value:1.12E-38 ID:SGTC_149|Compound:3970-0822|FD-Score:14.50|P-value:2.11E-38 ID:SGTC_158|Compound:k038-0087|FD-Score:-5.70|P-value:1.28E-7 ID:SGTC_236|Compound:1509-0011|FD-Score:3.96|P-value:2.36E-4 ID:SGTC_318|Compound:1488-0629|FD-Score:5.39|P-value:9.13E-7 ID:SGTC_381|Compound:3461-2219|FD-Score:4.88|P-value:7.88E-6 ID:SGTC_386|Compound:1509-0011|FD-Score:6.16|P-value:2.33E-8 ID:SGTC_399|Compound:oxolinic acid|FD-Score:3.84|P-value:3.57E-4 ID:SGTC_551|Compound:k048-0028|FD-Score:4.74|P-value:1.41E-5 ID:SGTC_600|Compound:1495-0418|FD-Score:7.67|P-value:4.82E-12 ID:SGTC_629|Compound:3448-6466|FD-Score:4.95|P-value:6.07E-6 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.58|P-value:8.10E-4 ID:SGTC_725|Compound:1497-0963|FD-Score:10.20|P-value:7.18E-20 ID:SGTC_740|Compound:k014-0057|FD-Score:5.35|P-value:1.09E-6 ID:SGTC_769|Compound:0302-0231|FD-Score:-3.76|P-value:3.21E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:8.36|P-value:5.44E-14 ID:SGTC_830|Compound:4076-0286|FD-Score:3.64|P-value:6.69E-4 ID:SGTC_879|Compound:0831-1030|FD-Score:4.60|P-value:2.40E-5 ID:SGTC_1047|Compound:2043-3044|FD-Score:3.62|P-value:7.17E-4 ID:SGTC_1147|Compound:3809-1045|FD-Score:4.63|P-value:2.12E-5 ID:SGTC_1173|Compound:0443-0020|FD-Score:4.76|P-value:1.30E-5 ID:SGTC_1179|Compound:1021-1431|FD-Score:4.07|P-value:1.64E-4 ID:SGTC_1256|Compound:0570-0116|FD-Score:4.32|P-value:6.83E-5 ID:SGTC_1259|Compound:0671-0132|FD-Score:7.27|P-value:5.34E-11 ID:SGTC_1264|Compound:0783-0040|FD-Score:4.39|P-value:5.28E-5 ID:SGTC_1304|Compound:1165-0502|FD-Score:7.95|P-value:7.93E-13 ID:SGTC_1316|Compound:1272-0050|FD-Score:4.02|P-value:1.97E-4 ID:SGTC_1385|Compound:3091-4834|FD-Score:3.73|P-value:4.99E-4 ID:SGTC_1399|Compound:3562-5772|FD-Score:8.08|P-value:3.53E-13 ID:SGTC_1420|Compound:4'-methoxyflavone|FD-Score:4.27|P-value:8.27E-5 ID:SGTC_1449|Compound:4112-4002|FD-Score:8.43|P-value:3.34E-14 ID:SGTC_1466|Compound:k213-0079|FD-Score:4.33|P-value:6.68E-5 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:4.47|P-value:3.88E-5 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.20|P-value:6.87E-5 ID:SGTC_1730|Compound:st033808|FD-Score:3.86|P-value:3.33E-4 ID:SGTC_1758|Compound:st042942|FD-Score:-3.74|P-value:3.41E-4 ID:SGTC_1766|Compound:st045414|FD-Score:19.70|P-value:1.18E-69 ID:SGTC_1834|Compound:st055981|FD-Score:9.83|P-value:1.09E-18 ID:SGTC_1844|Compound:rapamycin|FD-Score:-4.54|P-value:1.92E-5 ID:SGTC_1845|Compound:st056753|FD-Score:4.07|P-value:1.64E-4 ID:SGTC_1935|Compound:st074532|FD-Score:3.52|P-value:9.63E-4 ID:SGTC_1959|Compound:st077091|FD-Score:9.66|P-value:4.16E-18 ID:SGTC_2057|Compound:5236571|FD-Score:5.95|P-value:6.70E-8 ID:SGTC_2156|Compound:5649887|FD-Score:8.65|P-value:7.57E-15 ID:SGTC_2177|Compound:5811945|FD-Score:-3.53|P-value:6.61E-4 ID:SGTC_2179|Compound:6298986|FD-Score:-3.56|P-value:6.03E-4 ID:SGTC_2187|Compound:6125751|FD-Score:3.63|P-value:6.86E-4 ID:SGTC_2211|Compound:7117706|FD-Score:3.78|P-value:4.34E-4 ID:SGTC_2311|Compound:7694451|FD-Score:3.80|P-value:4.04E-4 ID:SGTC_2396|Compound:5342950|FD-Score:3.88|P-value:3.10E-4 ID:SGTC_2507|Compound:purpurin|FD-Score:6.53|P-value:3.50E-9 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:6.70|P-value:1.42E-9 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:3.57|P-value:8.32E-4 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:4.40|P-value:5.03E-5 ID:SGTC_2651|Compound:1327-0036|FD-Score:5.40|P-value:8.80E-7 ID:SGTC_2671|Compound:danthron|FD-Score:7.41|P-value:2.35E-11 ID:SGTC_2679|Compound:rapamycin|FD-Score:-8.05|P-value:2.02E-13 ID:SGTC_2686|Compound:alizarin|FD-Score:8.06|P-value:4.08E-13 ID:SGTC_2709|Compound:st078560|FD-Score:-3.69|P-value:4.06E-4 ID:SGTC_2805|Compound:7950783|FD-Score:6.26|P-value:1.45E-8 ID:SGTC_2810|Compound:7959871|FD-Score:12.20|P-value:1.05E-27 ID:SGTC_2813|Compound:7971799|FD-Score:4.66|P-value:1.90E-5 ID:SGTC_2846|Compound:9008642|FD-Score:4.19|P-value:1.08E-4 ID:SGTC_2921|Compound:7974240|FD-Score:5.19|P-value:2.19E-6 ID:SGTC_2926|Compound:9010726|FD-Score:12.40|P-value:1.17E-28 ID:SGTC_2969|Compound:9091408|FD-Score:-3.64|P-value:4.67E-4 ID:SGTC_2980|Compound:9014342|FD-Score:5.71|P-value:2.15E-7 ID:SGTC_3137|Compound:9097352|FD-Score:4.49|P-value:3.67E-5 ID:SGTC_3148|Compound:9097893|FD-Score:3.82|P-value:3.76E-4 ID:SGTC_3150|Compound:9097574|FD-Score:4.50|P-value:3.45E-5 ID:SGTC_3156|Compound:9099931|FD-Score:3.55|P-value:8.94E-4 ID:SGTC_3165|Compound:9101544|FD-Score:5.69|P-value:2.28E-7 ID:SGTC_3189|Compound:9110682|FD-Score:3.93|P-value:2.68E-4 ID:SGTC_3200|Compound:9114385|FD-Score:8.20|P-value:1.64E-13 ID:SGTC_3232|Compound:9132874|FD-Score:8.17|P-value:1.89E-13 ID:SGTC_3266|Compound:9136860|FD-Score:-5.67|P-value:1.50E-7 ID:SGTC_3328|Compound:9140624|FD-Score:-3.49|P-value:7.42E-4 ID:SGTC_23|Compound:0213-0011|FD-Score:4.28|P-value:7.76E-5 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.97|P-value:1.57E-4 ID:SGTC_100|Compound:0086-0128|FD-Score:7.07|P-value:1.70E-10 ID:SGTC_124|Compound:0136-0217|FD-Score:4.46|P-value:4.01E-5 ID:SGTC_129|Compound:0088-0017|FD-Score:5.08|P-value:3.52E-6 ID:SGTC_134|Compound:0269-0018|FD-Score:14.50|P-value:1.12E-38 ID:SGTC_149|Compound:3970-0822|FD-Score:14.50|P-value:2.11E-38 ID:SGTC_158|Compound:k038-0087|FD-Score:-5.70|P-value:1.28E-7 ID:SGTC_236|Compound:1509-0011|FD-Score:3.96|P-value:2.36E-4 ID:SGTC_318|Compound:1488-0629|FD-Score:5.39|P-value:9.13E-7 ID:SGTC_381|Compound:3461-2219|FD-Score:4.88|P-value:7.88E-6 ID:SGTC_386|Compound:1509-0011|FD-Score:6.16|P-value:2.33E-8 ID:SGTC_399|Compound:oxolinic acid|FD-Score:3.84|P-value:3.57E-4 ID:SGTC_551|Compound:k048-0028|FD-Score:4.74|P-value:1.41E-5 ID:SGTC_600|Compound:1495-0418|FD-Score:7.67|P-value:4.82E-12 ID:SGTC_629|Compound:3448-6466|FD-Score:4.95|P-value:6.07E-6 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.58|P-value:8.10E-4 ID:SGTC_725|Compound:1497-0963|FD-Score:10.20|P-value:7.18E-20 ID:SGTC_740|Compound:k014-0057|FD-Score:5.35|P-value:1.09E-6 ID:SGTC_769|Compound:0302-0231|FD-Score:-3.76|P-value:3.21E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:8.36|P-value:5.44E-14 ID:SGTC_830|Compound:4076-0286|FD-Score:3.64|P-value:6.69E-4 ID:SGTC_879|Compound:0831-1030|FD-Score:4.60|P-value:2.40E-5 ID:SGTC_1047|Compound:2043-3044|FD-Score:3.62|P-value:7.17E-4 ID:SGTC_1147|Compound:3809-1045|FD-Score:4.63|P-value:2.12E-5 ID:SGTC_1173|Compound:0443-0020|FD-Score:4.76|P-value:1.30E-5 ID:SGTC_1179|Compound:1021-1431|FD-Score:4.07|P-value:1.64E-4 ID:SGTC_1256|Compound:0570-0116|FD-Score:4.32|P-value:6.83E-5 ID:SGTC_1259|Compound:0671-0132|FD-Score:7.27|P-value:5.34E-11 ID:SGTC_1264|Compound:0783-0040|FD-Score:4.39|P-value:5.28E-5 ID:SGTC_1304|Compound:1165-0502|FD-Score:7.95|P-value:7.93E-13 ID:SGTC_1316|Compound:1272-0050|FD-Score:4.02|P-value:1.97E-4 ID:SGTC_1385|Compound:3091-4834|FD-Score:3.73|P-value:4.99E-4 ID:SGTC_1399|Compound:3562-5772|FD-Score:8.08|P-value:3.53E-13 ID:SGTC_1420|Compound:4'-methoxyflavone|FD-Score:4.27|P-value:8.27E-5 ID:SGTC_1449|Compound:4112-4002|FD-Score:8.43|P-value:3.34E-14 ID:SGTC_1466|Compound:k213-0079|FD-Score:4.33|P-value:6.68E-5 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:4.47|P-value:3.88E-5 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.20|P-value:6.87E-5 ID:SGTC_1730|Compound:st033808|FD-Score:3.86|P-value:3.33E-4 ID:SGTC_1758|Compound:st042942|FD-Score:-3.74|P-value:3.41E-4 ID:SGTC_1766|Compound:st045414|FD-Score:19.70|P-value:1.18E-69 ID:SGTC_1834|Compound:st055981|FD-Score:9.83|P-value:1.09E-18 ID:SGTC_1844|Compound:rapamycin|FD-Score:-4.54|P-value:1.92E-5 ID:SGTC_1845|Compound:st056753|FD-Score:4.07|P-value:1.64E-4 ID:SGTC_1935|Compound:st074532|FD-Score:3.52|P-value:9.63E-4 ID:SGTC_1959|Compound:st077091|FD-Score:9.66|P-value:4.16E-18 ID:SGTC_2057|Compound:5236571|FD-Score:5.95|P-value:6.70E-8 ID:SGTC_2156|Compound:5649887|FD-Score:8.65|P-value:7.57E-15 ID:SGTC_2177|Compound:5811945|FD-Score:-3.53|P-value:6.61E-4 ID:SGTC_2179|Compound:6298986|FD-Score:-3.56|P-value:6.03E-4 ID:SGTC_2187|Compound:6125751|FD-Score:3.63|P-value:6.86E-4 ID:SGTC_2211|Compound:7117706|FD-Score:3.78|P-value:4.34E-4 ID:SGTC_2311|Compound:7694451|FD-Score:3.80|P-value:4.04E-4 ID:SGTC_2396|Compound:5342950|FD-Score:3.88|P-value:3.10E-4 ID:SGTC_2507|Compound:purpurin|FD-Score:6.53|P-value:3.50E-9 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:6.70|P-value:1.42E-9 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:3.57|P-value:8.32E-4 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:4.40|P-value:5.03E-5 ID:SGTC_2651|Compound:1327-0036|FD-Score:5.40|P-value:8.80E-7 ID:SGTC_2671|Compound:danthron|FD-Score:7.41|P-value:2.35E-11 ID:SGTC_2679|Compound:rapamycin|FD-Score:-8.05|P-value:2.02E-13 ID:SGTC_2686|Compound:alizarin|FD-Score:8.06|P-value:4.08E-13 ID:SGTC_2709|Compound:st078560|FD-Score:-3.69|P-value:4.06E-4 ID:SGTC_2805|Compound:7950783|FD-Score:6.26|P-value:1.45E-8 ID:SGTC_2810|Compound:7959871|FD-Score:12.20|P-value:1.05E-27 ID:SGTC_2813|Compound:7971799|FD-Score:4.66|P-value:1.90E-5 ID:SGTC_2846|Compound:9008642|FD-Score:4.19|P-value:1.08E-4 ID:SGTC_2921|Compound:7974240|FD-Score:5.19|P-value:2.19E-6 ID:SGTC_2926|Compound:9010726|FD-Score:12.40|P-value:1.17E-28 ID:SGTC_2969|Compound:9091408|FD-Score:-3.64|P-value:4.67E-4 ID:SGTC_2980|Compound:9014342|FD-Score:5.71|P-value:2.15E-7 ID:SGTC_3137|Compound:9097352|FD-Score:4.49|P-value:3.67E-5 ID:SGTC_3148|Compound:9097893|FD-Score:3.82|P-value:3.76E-4 ID:SGTC_3150|Compound:9097574|FD-Score:4.50|P-value:3.45E-5 ID:SGTC_3156|Compound:9099931|FD-Score:3.55|P-value:8.94E-4 ID:SGTC_3165|Compound:9101544|FD-Score:5.69|P-value:2.28E-7 ID:SGTC_3189|Compound:9110682|FD-Score:3.93|P-value:2.68E-4 ID:SGTC_3200|Compound:9114385|FD-Score:8.20|P-value:1.64E-13 ID:SGTC_3232|Compound:9132874|FD-Score:8.17|P-value:1.89E-13 ID:SGTC_3266|Compound:9136860|FD-Score:-5.67|P-value:1.50E-7 ID:SGTC_3328|Compound:9140624|FD-Score:-3.49|P-value:7.42E-4

Top fitness defect scores for YNL236W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1766 st045414 19.70 44.8 µM 1.18E-69 RPP1 & pyrimidine depletion 1 6.09E-87
2 SGTC_134 0269-0018 14.50 6.9 µM 1.12E-38 20 3.70E-48
3 SGTC_149 3970-0822 14.50 46.1 µM 2.11E-38 tubulin folding & SWR complex 5 8.22E-48
4 SGTC_2926 9010726 12.40 65.2 µM 1.17E-28 1 1.31E-35
5 SGTC_2810 7959871 12.20 45.5 µM 1.05E-27 copper-dependent oxidative stress 2 2.06E-34
6 SGTC_725 1497-0963 10.20 19.0 µM 7.18E-20 copper-dependent oxidative stress 3 1.35E-24
7 SGTC_1834 st055981 9.83 52.8 µM 1.09E-18 RPP1 & pyrimidine depletion 1 4.04E-23
8 SGTC_1959 st077091 9.66 55.8 µM 4.16E-18 RPP1 & pyrimidine depletion 1 2.18E-22
9 SGTC_2156 5649887 8.65 115.0 µM 7.57E-15 RPP1 & pyrimidine depletion 1 2.65E-18
10 SGTC_1449 4112-4002 8.43 201.0 µM 3.34E-14 RPP1 & pyrimidine depletion 1 1.70E-17
11 SGTC_770 0389-1121 8.36 67.2 µM 5.44E-14 RPP1 & pyrimidine depletion 1 3.14E-17
12 SGTC_3200 9114385 8.20 49.5 µM 1.64E-13 1 1.25E-16
13 SGTC_3232 9132874 8.17 49.5 µM 1.89E-13 RPP1 & pyrimidine depletion 1 1.50E-16
14 SGTC_1399 3562-5772 8.08 25.0 µM 3.53E-13 RPP1 & pyrimidine depletion 1 3.27E-16
15 SGTC_2686 alizarin 8.06 95.4 µM 4.08E-13 RPP1 & pyrimidine depletion 2 3.92E-16
16 SGTC_1304 1165-0502 7.95 251.0 µM 7.93E-13 18 9.01E-16
17 SGTC_600 1495-0418 7.67 41.6 µM 4.82E-12 RPP1 & pyrimidine depletion 1 8.64E-15
18 SGTC_2671 danthron 7.41 100.0 µM 2.35E-11 DNA damage response 6 6.29E-14
19 SGTC_1259 0671-0132 7.27 43.0 µM 5.34E-11 2 1.76E-13
20 SGTC_100 0086-0128 7.07 49.6 µM 1.70E-10 copper-dependent oxidative stress 11 7.51E-13

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.338 3.86E-90 YKL052C ASK1 het Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress
0.267 1.37E-55 YGL025C PGD1 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor
0.216 1.30E-36 YDR231C COX20 hom Mitochondrial inner membrane protein, required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase
0.190 1.79E-28 YNL235C_d YNL235C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex
0.170 3.81E-23 YML129C COX14 hom Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes
0.168 1.06E-22 YPL132W COX11 hom Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p
0.168 1.73E-22 YDL067C COX9 hom Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain
0.165 6.97E-22 YIR021W MRS1 hom Protein required for the splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA
0.161 6.66E-21 YBR257W POP4 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
0.160 1.03E-20 YJL209W CBP1 hom Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress
0.160 1.16E-20 YLR044C PDC1 hom Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism
0.159 2.33E-20 YOL051W GAL11 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator
0.158 4.47E-20 YDL107W MSS2 hom Peripherally bound inner membrane protein of the mitochondrial matrix involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p
0.156 1.40E-19 YNR045W PET494 hom Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane
0.155 2.10E-19 YER025W GCD11 het Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met