YNL242W / ATG2

Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL242W / ATG2

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:5.31|P-value:1.82E-8 ID:SGTC_237|Compound:nikkomycin|FD-Score:4.01|P-value:1.60E-5 ID:SGTC_323|Compound:4013-0906|FD-Score:3.53|P-value:1.27E-4 ID:SGTC_448|Compound:monastrol|FD-Score:6.80|P-value:9.52E-13 ID:SGTC_451|Compound:chloral hydrate|FD-Score:4.11|P-value:1.04E-5 ID:SGTC_452|Compound:diindolylmethane|FD-Score:-5.21|P-value:3.51E-8 ID:SGTC_572|Compound:0960-0087|FD-Score:3.32|P-value:2.88E-4 ID:SGTC_663|Compound:1486-0733|FD-Score:3.04|P-value:8.07E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:3.18|P-value:4.92E-4 ID:SGTC_726|Compound:3448-4391|FD-Score:3.33|P-value:2.73E-4 ID:SGTC_751|Compound:0833-0568|FD-Score:5.97|P-value:3.05E-10 ID:SGTC_1009|Compound:4106-0057|FD-Score:3.80|P-value:4.15E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.85|P-value:3.44E-5 ID:SGTC_1122|Compound:capsaicin|FD-Score:-3.02|P-value:9.10E-4 ID:SGTC_1221|Compound:2958-7315|FD-Score:3.28|P-value:3.39E-4 ID:SGTC_1302|Compound:1159-0335|FD-Score:3.10|P-value:6.55E-4 ID:SGTC_1431|Compound:4049-0260|FD-Score:3.10|P-value:6.43E-4 ID:SGTC_1447|Compound:4239-0816|FD-Score:3.54|P-value:1.22E-4 ID:SGTC_1871|Compound:st057448|FD-Score:3.15|P-value:5.48E-4 ID:SGTC_1962|Compound:st076595|FD-Score:4.44|P-value:2.07E-6 ID:SGTC_2064|Compound:5220758|FD-Score:3.08|P-value:7.13E-4 ID:SGTC_2146|Compound:5545642|FD-Score:3.03|P-value:8.30E-4 ID:SGTC_2163|Compound:5671233|FD-Score:3.32|P-value:2.90E-4 ID:SGTC_2346|Compound:9031566|FD-Score:-3.02|P-value:8.97E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:5.61|P-value:3.09E-9 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:3.62|P-value:8.92E-5 ID:SGTC_2652|Compound:actinonin|FD-Score:3.03|P-value:8.53E-4 ID:SGTC_2674|Compound:ligustilide|FD-Score:3.15|P-value:5.43E-4 ID:SGTC_3165|Compound:9101544|FD-Score:4.97|P-value:1.31E-7 ID:SGTC_3295|Compound:9122362|FD-Score:3.02|P-value:8.81E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:5.31|P-value:1.82E-8 ID:SGTC_237|Compound:nikkomycin|FD-Score:4.01|P-value:1.60E-5 ID:SGTC_323|Compound:4013-0906|FD-Score:3.53|P-value:1.27E-4 ID:SGTC_448|Compound:monastrol|FD-Score:6.80|P-value:9.52E-13 ID:SGTC_451|Compound:chloral hydrate|FD-Score:4.11|P-value:1.04E-5 ID:SGTC_452|Compound:diindolylmethane|FD-Score:-5.21|P-value:3.51E-8 ID:SGTC_572|Compound:0960-0087|FD-Score:3.32|P-value:2.88E-4 ID:SGTC_663|Compound:1486-0733|FD-Score:3.04|P-value:8.07E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:3.18|P-value:4.92E-4 ID:SGTC_726|Compound:3448-4391|FD-Score:3.33|P-value:2.73E-4 ID:SGTC_751|Compound:0833-0568|FD-Score:5.97|P-value:3.05E-10 ID:SGTC_1009|Compound:4106-0057|FD-Score:3.80|P-value:4.15E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.85|P-value:3.44E-5 ID:SGTC_1122|Compound:capsaicin|FD-Score:-3.02|P-value:9.10E-4 ID:SGTC_1221|Compound:2958-7315|FD-Score:3.28|P-value:3.39E-4 ID:SGTC_1302|Compound:1159-0335|FD-Score:3.10|P-value:6.55E-4 ID:SGTC_1431|Compound:4049-0260|FD-Score:3.10|P-value:6.43E-4 ID:SGTC_1447|Compound:4239-0816|FD-Score:3.54|P-value:1.22E-4 ID:SGTC_1871|Compound:st057448|FD-Score:3.15|P-value:5.48E-4 ID:SGTC_1962|Compound:st076595|FD-Score:4.44|P-value:2.07E-6 ID:SGTC_2064|Compound:5220758|FD-Score:3.08|P-value:7.13E-4 ID:SGTC_2146|Compound:5545642|FD-Score:3.03|P-value:8.30E-4 ID:SGTC_2163|Compound:5671233|FD-Score:3.32|P-value:2.90E-4 ID:SGTC_2346|Compound:9031566|FD-Score:-3.02|P-value:8.97E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:5.61|P-value:3.09E-9 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:3.62|P-value:8.92E-5 ID:SGTC_2652|Compound:actinonin|FD-Score:3.03|P-value:8.53E-4 ID:SGTC_2674|Compound:ligustilide|FD-Score:3.15|P-value:5.43E-4 ID:SGTC_3165|Compound:9101544|FD-Score:4.97|P-value:1.31E-7 ID:SGTC_3295|Compound:9122362|FD-Score:3.02|P-value:8.81E-4

Top fitness defect scores for YNL242W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_448 monastrol 6.80 171.0 µM 9.52E-13 1 5.38E-12
2 SGTC_751 0833-0568 5.97 350.0 µM 3.05E-10 1 1.18E-9
3 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 5.61 3.7 µM 3.09E-9 16 1.02E-8
4 SGTC_6 0844-0013 5.31 19.8 µM 1.82E-8 28 5.35E-8
5 SGTC_3165 9101544 4.97 49.5 µM 1.31E-7 4 3.37E-7
6 SGTC_1962 st076595 4.44 64.5 µM 2.07E-6 redox potentiating 18 4.46E-6
7 SGTC_451 chloral hydrate 4.11 100.0 µM 1.04E-5 45 2.01E-5
8 SGTC_237 nikkomycin 4.01 200.0 µM 1.60E-5 RSC complex & mRNA processing 12 3.01E-5
9 SGTC_1009 4106-0057 3.80 9.9 µM 4.15E-5 15 7.34E-5
10 SGTC_2565 isoliquiritigenin 3.62 100.0 µM 8.92E-5 41 1.50E-4
11 SGTC_1447 4239-0816 3.54 81.9 µM 1.22E-4 44 2.01E-4
12 SGTC_323 4013-0906 3.53 34.3 µM 1.27E-4 ERG2 15 2.09E-4
13 SGTC_726 3448-4391 3.33 34.2 µM 2.73E-4 35 4.27E-4
14 SGTC_572 0960-0087 3.32 17.7 µM 2.88E-4 56 4.49E-4
15 SGTC_2163 5671233 3.32 125.0 µM 2.90E-4 45 4.53E-4
16 SGTC_1221 2958-7315 3.28 257.0 µM 3.39E-4 35 5.24E-4
17 SGTC_673 1082-0474 3.18 110.0 µM 4.92E-4 RSC complex & mRNA processing 107 7.43E-4
18 SGTC_2674 ligustilide 3.15 100.0 µM 5.43E-4 26 8.13E-4
19 SGTC_1871 st057448 3.15 67.3 µM 5.48E-4 53 8.21E-4
20 SGTC_1431 4049-0260 3.10 9.1 µM 6.43E-4 32 9.53E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.242 8.16E-46 YGL004C RPN14 hom Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p
0.205 4.68E-33 YLR398C SKI2 hom Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay
0.204 9.79E-33 YGL017W ATE1 hom Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway
0.201 8.27E-32 YDL049C KNH1 hom Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
0.199 2.71E-31 YER067C-A_d YER067C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W
0.191 5.28E-29 YLR026C SED5 het cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins
0.190 1.04E-28 YLR288C MEC3 hom DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1
0.188 4.00E-28 YFL046W_p FMP32_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.163 2.77E-21 YMR173W DDR48 hom DNA damage-responsive protein; expression is increased in response to heat-shock stress or treatments that produce DNA lesions; contains multiple repeats of the amino acid sequence NNNDSYGS; protein abundance increases in response to DNA replication stress
0.158 2.94E-20 YOR155C ISN1 hom Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms
0.157 4.78E-20 YDR051C DET1 hom Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel
0.157 7.30E-20 YHR021W-A_p ECM12_p hom Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity
0.152 7.28E-19 YDR068W DOS2 hom Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.150 2.66E-18 YML100W TSL1 hom Large subunit of trehalose 6-phosphate synthase/phosphatase complex; Tps1p-Tps2p complex converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress; TSL1 has a paralog, TPS3, that arose from the whole genome duplication
0.148 7.54E-18 YOR300W_d YOR300W_d hom Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7