YNL250W / RAD50

Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL250W / RAD50

Click on Significant Values for Screen Details ID:SGTC_31|Compound:0508-1974|FD-Score:3.30|P-value:2.50E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:2.95|P-value:8.72E-4 ID:SGTC_115|Compound:0527-0192|FD-Score:6.63|P-value:6.94E-12 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:4.80|P-value:3.56E-7 ID:SGTC_244|Compound:nsc-207895|FD-Score:3.29|P-value:2.65E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:-4.47|P-value:1.47E-5 ID:SGTC_411|Compound:enrofloxacin|FD-Score:3.44|P-value:1.49E-4 ID:SGTC_427|Compound:1354-0053|FD-Score:-4.09|P-value:7.15E-5 ID:SGTC_450|Compound:aminopyrene|FD-Score:3.13|P-value:4.62E-4 ID:SGTC_605|Compound:1315-0399|FD-Score:3.51|P-value:1.14E-4 ID:SGTC_670|Compound:4204-0025|FD-Score:4.59|P-value:1.02E-6 ID:SGTC_679|Compound:0422-0091|FD-Score:4.28|P-value:4.37E-6 ID:SGTC_745|Compound:0469-0705|FD-Score:3.30|P-value:2.55E-4 ID:SGTC_864|Compound:0469-0573|FD-Score:3.23|P-value:3.29E-4 ID:SGTC_870|Compound:0573-2213|FD-Score:3.51|P-value:1.16E-4 ID:SGTC_904|Compound:1141-0088|FD-Score:3.49|P-value:1.26E-4 ID:SGTC_955|Compound:1216-0110|FD-Score:5.80|P-value:1.44E-9 ID:SGTC_1052|Compound:3253-3998|FD-Score:2.95|P-value:8.81E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:3.03|P-value:6.55E-4 ID:SGTC_1105|Compound:epirubicin|FD-Score:3.41|P-value:1.71E-4 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:6.23|P-value:9.76E-11 ID:SGTC_1117|Compound:retinoic acid|FD-Score:3.69|P-value:5.56E-5 ID:SGTC_1357|Compound:1503-1303|FD-Score:3.37|P-value:1.97E-4 ID:SGTC_1454|Compound:4239-0054|FD-Score:3.21|P-value:3.55E-4 ID:SGTC_1664|Compound:st014217|FD-Score:3.22|P-value:3.44E-4 ID:SGTC_1707|Compound:st032323|FD-Score:4.55|P-value:1.26E-6 ID:SGTC_1746|Compound:st038117|FD-Score:6.09|P-value:2.47E-10 ID:SGTC_1845|Compound:st056753|FD-Score:4.80|P-value:3.55E-7 ID:SGTC_1930|Compound:st074634|FD-Score:6.52|P-value:1.47E-11 ID:SGTC_1998|Compound:st075797|FD-Score:2.97|P-value:8.19E-4 ID:SGTC_1999|Compound:st074831|FD-Score:-3.45|P-value:7.60E-4 ID:SGTC_2318|Compound:6029776|FD-Score:4.03|P-value:1.35E-5 ID:SGTC_2401|Compound:5128683|FD-Score:4.34|P-value:3.31E-6 ID:SGTC_2488|Compound:5268135|FD-Score:3.25|P-value:3.02E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:3.98|P-value:1.71E-5 ID:SGTC_2612|Compound:dictamnine|FD-Score:3.76|P-value:4.32E-5 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:-4.10|P-value:6.89E-5 ID:SGTC_2619|Compound:obliquin|FD-Score:4.96|P-value:1.56E-7 ID:SGTC_2671|Compound:danthron|FD-Score:6.48|P-value:1.94E-11 ID:SGTC_2691|Compound:st077356|FD-Score:4.92|P-value:1.96E-7 ID:SGTC_2738|Compound:aclarubicin|FD-Score:3.44|P-value:1.51E-4 ID:SGTC_2747|Compound:doxorubicin|FD-Score:3.76|P-value:4.18E-5 ID:SGTC_2782|Compound:7736369|FD-Score:4.06|P-value:1.22E-5 ID:SGTC_2840|Compound:9008771|FD-Score:3.15|P-value:4.42E-4 ID:SGTC_2877|Compound:9040724|FD-Score:-3.64|P-value:3.86E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-4.82|P-value:2.99E-6 ID:SGTC_3351|Compound:9150312|FD-Score:3.32|P-value:2.36E-4 ID:SGTC_31|Compound:0508-1974|FD-Score:3.30|P-value:2.50E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:2.95|P-value:8.72E-4 ID:SGTC_115|Compound:0527-0192|FD-Score:6.63|P-value:6.94E-12 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:4.80|P-value:3.56E-7 ID:SGTC_244|Compound:nsc-207895|FD-Score:3.29|P-value:2.65E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:-4.47|P-value:1.47E-5 ID:SGTC_411|Compound:enrofloxacin|FD-Score:3.44|P-value:1.49E-4 ID:SGTC_427|Compound:1354-0053|FD-Score:-4.09|P-value:7.15E-5 ID:SGTC_450|Compound:aminopyrene|FD-Score:3.13|P-value:4.62E-4 ID:SGTC_605|Compound:1315-0399|FD-Score:3.51|P-value:1.14E-4 ID:SGTC_670|Compound:4204-0025|FD-Score:4.59|P-value:1.02E-6 ID:SGTC_679|Compound:0422-0091|FD-Score:4.28|P-value:4.37E-6 ID:SGTC_745|Compound:0469-0705|FD-Score:3.30|P-value:2.55E-4 ID:SGTC_864|Compound:0469-0573|FD-Score:3.23|P-value:3.29E-4 ID:SGTC_870|Compound:0573-2213|FD-Score:3.51|P-value:1.16E-4 ID:SGTC_904|Compound:1141-0088|FD-Score:3.49|P-value:1.26E-4 ID:SGTC_955|Compound:1216-0110|FD-Score:5.80|P-value:1.44E-9 ID:SGTC_1052|Compound:3253-3998|FD-Score:2.95|P-value:8.81E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:3.03|P-value:6.55E-4 ID:SGTC_1105|Compound:epirubicin|FD-Score:3.41|P-value:1.71E-4 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:6.23|P-value:9.76E-11 ID:SGTC_1117|Compound:retinoic acid|FD-Score:3.69|P-value:5.56E-5 ID:SGTC_1357|Compound:1503-1303|FD-Score:3.37|P-value:1.97E-4 ID:SGTC_1454|Compound:4239-0054|FD-Score:3.21|P-value:3.55E-4 ID:SGTC_1664|Compound:st014217|FD-Score:3.22|P-value:3.44E-4 ID:SGTC_1707|Compound:st032323|FD-Score:4.55|P-value:1.26E-6 ID:SGTC_1746|Compound:st038117|FD-Score:6.09|P-value:2.47E-10 ID:SGTC_1845|Compound:st056753|FD-Score:4.80|P-value:3.55E-7 ID:SGTC_1930|Compound:st074634|FD-Score:6.52|P-value:1.47E-11 ID:SGTC_1998|Compound:st075797|FD-Score:2.97|P-value:8.19E-4 ID:SGTC_1999|Compound:st074831|FD-Score:-3.45|P-value:7.60E-4 ID:SGTC_2318|Compound:6029776|FD-Score:4.03|P-value:1.35E-5 ID:SGTC_2401|Compound:5128683|FD-Score:4.34|P-value:3.31E-6 ID:SGTC_2488|Compound:5268135|FD-Score:3.25|P-value:3.02E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:3.98|P-value:1.71E-5 ID:SGTC_2612|Compound:dictamnine|FD-Score:3.76|P-value:4.32E-5 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:-4.10|P-value:6.89E-5 ID:SGTC_2619|Compound:obliquin|FD-Score:4.96|P-value:1.56E-7 ID:SGTC_2671|Compound:danthron|FD-Score:6.48|P-value:1.94E-11 ID:SGTC_2691|Compound:st077356|FD-Score:4.92|P-value:1.96E-7 ID:SGTC_2738|Compound:aclarubicin|FD-Score:3.44|P-value:1.51E-4 ID:SGTC_2747|Compound:doxorubicin|FD-Score:3.76|P-value:4.18E-5 ID:SGTC_2782|Compound:7736369|FD-Score:4.06|P-value:1.22E-5 ID:SGTC_2840|Compound:9008771|FD-Score:3.15|P-value:4.42E-4 ID:SGTC_2877|Compound:9040724|FD-Score:-3.64|P-value:3.86E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-4.82|P-value:2.99E-6 ID:SGTC_3351|Compound:9150312|FD-Score:3.32|P-value:2.36E-4

Top fitness defect scores for YNL250W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_115 0527-0192 6.63 112.0 µM 6.94E-12 12 1.66E-11
2 SGTC_1930 st074634 6.52 34.3 µM 1.47E-11 RNA pol III & RNase P/MRP 7 3.51E-11
3 SGTC_2671 danthron 6.48 100.0 µM 1.94E-11 DNA damage response 10 4.63E-11
4 SGTC_1106 tegaserod maleate 6.23 11.7 µM 9.76E-11 1 2.30E-10
5 SGTC_1746 st038117 6.09 42.0 µM 2.47E-10 6 5.80E-10
6 SGTC_955 1216-0110 5.80 33.0 µM 1.44E-9 sphingolipid biosynthesis & PDR1 33 3.34E-9
7 SGTC_2619 obliquin 4.96 87.9 µM 1.56E-7 cell wall 99 3.46E-7
8 SGTC_2691 st077356 4.92 60.2 µM 1.96E-7 14 4.31E-7
9 SGTC_1845 st056753 4.80 3.0 µM 3.55E-7 TSC3-RPN4 20 7.78E-7
10 SGTC_219 mitoxanthrone 4.80 164.4 µM 3.56E-7 DNA intercalators 32 7.78E-7
11 SGTC_670 4204-0025 4.59 119.0 µM 1.02E-6 copper-dependent oxidative stress 18 2.20E-6
12 SGTC_1707 st032323 4.55 6.3 µM 1.26E-6 DNA damage response 44 2.71E-6
13 SGTC_2401 5128683 4.34 200.0 µM 3.31E-6 29 6.97E-6
14 SGTC_679 0422-0091 4.28 41.4 µM 4.37E-6 Golgi 23 9.17E-6
15 SGTC_2782 7736369 4.06 71.4 µM 1.22E-5 36 2.51E-5
16 SGTC_2318 6029776 4.03 164.0 µM 1.35E-5 31 2.78E-5
17 SGTC_2544 sphondin 3.98 68.5 µM 1.71E-5 RNA pol III & RNase P/MRP 25 3.48E-5
18 SGTC_2747 doxorubicin 3.76 7.6 µM 4.18E-5 anthracycline transcription coupled DNA repair 6 8.35E-5
19 SGTC_2612 dictamnine 3.76 15.5 µM 4.32E-5 RNA pol III & RNase P/MRP 48 8.63E-5
20 SGTC_1117 retinoic acid 3.69 1.1 µM 5.56E-5 24 1.10E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.225 6.92E-40 YOR116C RPO31 het RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
0.213 1.02E-35 YBR098W MMS4 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.207 7.77E-34 YDL059C RAD59 hom Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p
0.200 1.15E-31 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.194 9.07E-30 YDR076W RAD55 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
0.190 1.74E-28 YGL163C RAD54 hom DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress
0.170 4.19E-23 YDR004W RAD57 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
0.158 3.49E-20 YGL175C SAE2 hom Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents
0.142 1.31E-16 YBR099C_d YBR099C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
0.135 5.32E-15 YDL150W RPC53 het RNA polymerase III subunit C53
0.129 6.51E-14 YJL159W HSP150 hom O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.124 6.10E-13 YJL047C RTT101 hom Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p
0.118 5.86E-12 YER095W RAD51 hom Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein
0.116 1.73E-11 YPL056C_p LCL1_p hom Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan
0.113 6.67E-11 YGR070W ROM1 hom GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP