YNL251C / NRD1

RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites

Zygosity: Heterozygous strain
fixedexpanded
Profile for YNL251C / NRD1

Click on Significant Values for Screen Details ID:SGTC_79|Compound:0052-0007|FD-Score:-3.12|P-value:5.13E-4 ID:SGTC_82|Compound:0054-0107|FD-Score:-3.15|P-value:4.55E-4 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:-3.45|P-value:1.42E-4 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:4.01|P-value:1.36E-5 ID:SGTC_173|Compound:1273-0061|FD-Score:-3.44|P-value:1.48E-4 ID:SGTC_176|Compound:1486-1328|FD-Score:2.97|P-value:9.66E-4 ID:SGTC_183|Compound:3914-0051|FD-Score:3.56|P-value:9.75E-5 ID:SGTC_298|Compound:1391-0729|FD-Score:2.99|P-value:9.00E-4 ID:SGTC_520|Compound:3584-0023|FD-Score:-4.32|P-value:2.73E-6 ID:SGTC_538|Compound:2922-0838|FD-Score:3.12|P-value:5.44E-4 ID:SGTC_545|Compound:4218-0007|FD-Score:-3.16|P-value:4.46E-4 ID:SGTC_554|Compound:4483-2211|FD-Score:3.11|P-value:5.66E-4 ID:SGTC_587|Compound:k089-0003|FD-Score:-2.99|P-value:8.29E-4 ID:SGTC_622|Compound:1348-1566|FD-Score:5.72|P-value:1.01E-9 ID:SGTC_690|Compound:glyoxal|FD-Score:3.24|P-value:3.54E-4 ID:SGTC_729|Compound:4092-0585|FD-Score:2.96|P-value:9.70E-4 ID:SGTC_773|Compound:4358-1457|FD-Score:3.73|P-value:4.79E-5 ID:SGTC_777|Compound:1315-0167|FD-Score:-3.65|P-value:6.23E-5 ID:SGTC_786|Compound:4544-0069|FD-Score:3.70|P-value:5.49E-5 ID:SGTC_797|Compound:k781-0471|FD-Score:3.05|P-value:7.09E-4 ID:SGTC_801|Compound:0866-0028|FD-Score:3.44|P-value:1.62E-4 ID:SGTC_803|Compound:4488-1585|FD-Score:4.24|P-value:4.44E-6 ID:SGTC_855|Compound:0428-0020|FD-Score:5.18|P-value:2.95E-8 ID:SGTC_881|Compound:0833-0569|FD-Score:3.90|P-value:2.20E-5 ID:SGTC_918|Compound:glabranin|FD-Score:3.87|P-value:2.53E-5 ID:SGTC_1003|Compound:4049-0218|FD-Score:-3.31|P-value:2.42E-4 ID:SGTC_1066|Compound:ketoconazole|FD-Score:3.14|P-value:5.21E-4 ID:SGTC_1155|Compound:4088-0394|FD-Score:-3.02|P-value:7.34E-4 ID:SGTC_1156|Compound:4092-0367|FD-Score:3.22|P-value:3.78E-4 ID:SGTC_1216|Compound:1165-0504|FD-Score:3.68|P-value:5.82E-5 ID:SGTC_1273|Compound:0849-0096|FD-Score:3.90|P-value:2.26E-5 ID:SGTC_1275|Compound:0867-0008|FD-Score:4.01|P-value:1.36E-5 ID:SGTC_1278|Compound:0874-0585|FD-Score:3.52|P-value:1.15E-4 ID:SGTC_1285|Compound:0929-0069|FD-Score:-3.03|P-value:7.19E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:3.69|P-value:5.66E-5 ID:SGTC_1596|Compound:piperlongumine|FD-Score:4.86|P-value:1.76E-7 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:4.95|P-value:1.11E-7 ID:SGTC_1689|Compound:st020265|FD-Score:-3.40|P-value:1.76E-4 ID:SGTC_1712|Compound:st032288|FD-Score:3.90|P-value:2.20E-5 ID:SGTC_1754|Compound:st042065|FD-Score:4.31|P-value:3.28E-6 ID:SGTC_1824|Compound:st053723|FD-Score:3.35|P-value:2.26E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.05|P-value:7.05E-4 ID:SGTC_1847|Compound:st056231|FD-Score:-3.81|P-value:3.10E-5 ID:SGTC_1854|Compound:st056233|FD-Score:3.80|P-value:3.52E-5 ID:SGTC_1861|Compound:st057647|FD-Score:3.76|P-value:4.18E-5 ID:SGTC_1890|Compound:3',4',7',8'-tetrahydroxyflavone|FD-Score:3.73|P-value:4.69E-5 ID:SGTC_1976|Compound:st070609|FD-Score:3.05|P-value:7.17E-4 ID:SGTC_2081|Compound:5214970|FD-Score:-2.96|P-value:9.29E-4 ID:SGTC_2348|Compound:9022543|FD-Score:4.55|P-value:9.33E-7 ID:SGTC_2358|Compound:9037157|FD-Score:-3.07|P-value:6.07E-4 ID:SGTC_2488|Compound:5268135|FD-Score:3.38|P-value:2.00E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.15|P-value:4.91E-4 ID:SGTC_2625|Compound:euphol|FD-Score:3.48|P-value:1.34E-4 ID:SGTC_2687|Compound:chenodiol|FD-Score:3.22|P-value:3.78E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.61|P-value:2.07E-9 ID:SGTC_2717|Compound:st081029|FD-Score:4.56|P-value:9.09E-7 ID:SGTC_2860|Compound:9025725|FD-Score:3.09|P-value:6.09E-4 ID:SGTC_2972|Compound:9093619|FD-Score:3.61|P-value:8.04E-5 ID:SGTC_3006|Compound:9075424|FD-Score:-3.50|P-value:1.13E-4 ID:SGTC_79|Compound:0052-0007|FD-Score:-3.12|P-value:5.13E-4 ID:SGTC_82|Compound:0054-0107|FD-Score:-3.15|P-value:4.55E-4 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:-3.45|P-value:1.42E-4 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:4.01|P-value:1.36E-5 ID:SGTC_173|Compound:1273-0061|FD-Score:-3.44|P-value:1.48E-4 ID:SGTC_176|Compound:1486-1328|FD-Score:2.97|P-value:9.66E-4 ID:SGTC_183|Compound:3914-0051|FD-Score:3.56|P-value:9.75E-5 ID:SGTC_298|Compound:1391-0729|FD-Score:2.99|P-value:9.00E-4 ID:SGTC_520|Compound:3584-0023|FD-Score:-4.32|P-value:2.73E-6 ID:SGTC_538|Compound:2922-0838|FD-Score:3.12|P-value:5.44E-4 ID:SGTC_545|Compound:4218-0007|FD-Score:-3.16|P-value:4.46E-4 ID:SGTC_554|Compound:4483-2211|FD-Score:3.11|P-value:5.66E-4 ID:SGTC_587|Compound:k089-0003|FD-Score:-2.99|P-value:8.29E-4 ID:SGTC_622|Compound:1348-1566|FD-Score:5.72|P-value:1.01E-9 ID:SGTC_690|Compound:glyoxal|FD-Score:3.24|P-value:3.54E-4 ID:SGTC_729|Compound:4092-0585|FD-Score:2.96|P-value:9.70E-4 ID:SGTC_773|Compound:4358-1457|FD-Score:3.73|P-value:4.79E-5 ID:SGTC_777|Compound:1315-0167|FD-Score:-3.65|P-value:6.23E-5 ID:SGTC_786|Compound:4544-0069|FD-Score:3.70|P-value:5.49E-5 ID:SGTC_797|Compound:k781-0471|FD-Score:3.05|P-value:7.09E-4 ID:SGTC_801|Compound:0866-0028|FD-Score:3.44|P-value:1.62E-4 ID:SGTC_803|Compound:4488-1585|FD-Score:4.24|P-value:4.44E-6 ID:SGTC_855|Compound:0428-0020|FD-Score:5.18|P-value:2.95E-8 ID:SGTC_881|Compound:0833-0569|FD-Score:3.90|P-value:2.20E-5 ID:SGTC_918|Compound:glabranin|FD-Score:3.87|P-value:2.53E-5 ID:SGTC_1003|Compound:4049-0218|FD-Score:-3.31|P-value:2.42E-4 ID:SGTC_1066|Compound:ketoconazole|FD-Score:3.14|P-value:5.21E-4 ID:SGTC_1155|Compound:4088-0394|FD-Score:-3.02|P-value:7.34E-4 ID:SGTC_1156|Compound:4092-0367|FD-Score:3.22|P-value:3.78E-4 ID:SGTC_1216|Compound:1165-0504|FD-Score:3.68|P-value:5.82E-5 ID:SGTC_1273|Compound:0849-0096|FD-Score:3.90|P-value:2.26E-5 ID:SGTC_1275|Compound:0867-0008|FD-Score:4.01|P-value:1.36E-5 ID:SGTC_1278|Compound:0874-0585|FD-Score:3.52|P-value:1.15E-4 ID:SGTC_1285|Compound:0929-0069|FD-Score:-3.03|P-value:7.19E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:3.69|P-value:5.66E-5 ID:SGTC_1596|Compound:piperlongumine|FD-Score:4.86|P-value:1.76E-7 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:4.95|P-value:1.11E-7 ID:SGTC_1689|Compound:st020265|FD-Score:-3.40|P-value:1.76E-4 ID:SGTC_1712|Compound:st032288|FD-Score:3.90|P-value:2.20E-5 ID:SGTC_1754|Compound:st042065|FD-Score:4.31|P-value:3.28E-6 ID:SGTC_1824|Compound:st053723|FD-Score:3.35|P-value:2.26E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.05|P-value:7.05E-4 ID:SGTC_1847|Compound:st056231|FD-Score:-3.81|P-value:3.10E-5 ID:SGTC_1854|Compound:st056233|FD-Score:3.80|P-value:3.52E-5 ID:SGTC_1861|Compound:st057647|FD-Score:3.76|P-value:4.18E-5 ID:SGTC_1890|Compound:3',4',7',8'-tetrahydroxyflavone|FD-Score:3.73|P-value:4.69E-5 ID:SGTC_1976|Compound:st070609|FD-Score:3.05|P-value:7.17E-4 ID:SGTC_2081|Compound:5214970|FD-Score:-2.96|P-value:9.29E-4 ID:SGTC_2348|Compound:9022543|FD-Score:4.55|P-value:9.33E-7 ID:SGTC_2358|Compound:9037157|FD-Score:-3.07|P-value:6.07E-4 ID:SGTC_2488|Compound:5268135|FD-Score:3.38|P-value:2.00E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.15|P-value:4.91E-4 ID:SGTC_2625|Compound:euphol|FD-Score:3.48|P-value:1.34E-4 ID:SGTC_2687|Compound:chenodiol|FD-Score:3.22|P-value:3.78E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.61|P-value:2.07E-9 ID:SGTC_2717|Compound:st081029|FD-Score:4.56|P-value:9.09E-7 ID:SGTC_2860|Compound:9025725|FD-Score:3.09|P-value:6.09E-4 ID:SGTC_2972|Compound:9093619|FD-Score:3.61|P-value:8.04E-5 ID:SGTC_3006|Compound:9075424|FD-Score:-3.50|P-value:1.13E-4

Top fitness defect scores for YNL251C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_622 1348-1566 5.72 26.3 µM 1.01E-9 1 5.20E-9 1.34
2 SGTC_2688 kanamycin b 5.61 96.7 µM 2.07E-9 calcium & mitochondrial duress 1 9.95E-9 0.46
3 SGTC_855 0428-0020 5.18 25.0 µM 2.95E-8 Golgi 4 1.13E-7 0.11
4 SGTC_1597 7-benzyloxygramine 4.95 47.5 µM 1.11E-7 calcium & mitochondrial duress 42 3.77E-7 0.12
5 SGTC_1596 piperlongumine 4.86 63.0 µM 1.76E-7 2 5.75E-7 1.25
6 SGTC_2717 st081029 4.56 62.3 µM 9.09E-7 2 2.57E-6 0.70
7 SGTC_2348 9022543 4.55 33.4 µM 9.33E-7 mitochondrial processes 26 2.64E-6 0.05
8 SGTC_1754 st042065 4.31 16.1 µM 3.28E-6 NEO1-PIK1 25 8.32E-6 0.00
9 SGTC_803 4488-1585 4.24 147.0 µM 4.44E-6 2 1.10E-5 0.37
10 SGTC_120 rhodamine 6G 4.01 52.3 µM 1.36E-5 mitochondrial processes 17 3.05E-5 0.09
11 SGTC_1275 0867-0008 4.01 21.1 µM 1.36E-5 5 3.06E-5 0.30
12 SGTC_1712 st032288 3.90 53.4 µM 2.20E-5 calcium & mitochondrial duress 37 4.73E-5 0.10
13 SGTC_881 0833-0569 3.90 170.0 µM 2.20E-5 4 4.74E-5 0.28
14 SGTC_1273 0849-0096 3.90 14.1 µM 2.26E-5 RPP1 & pyrimidine depletion 8 4.85E-5 0.11
15 SGTC_918 glabranin 3.87 770.7 nM 2.53E-5 8 5.39E-5 0.06
16 SGTC_1854 st056233 3.80 32.9 µM 3.52E-5 amide catabolism 3 7.28E-5 0.18
17 SGTC_1861 st057647 3.76 17.4 µM 4.18E-5 6 8.53E-5 0.00
18 SGTC_1890 3',4',7',8'-tetrahydroxyflavone 3.73 14.9 µM 4.69E-5 7 9.47E-5 0.11
19 SGTC_773 4358-1457 3.73 44.6 µM 4.79E-5 10 9.66E-5 0.03
20 SGTC_786 4544-0069 3.70 423.0 µM 5.49E-5 9 1.09E-4 0.16

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.169 6.66E-23 YKL051W SFK1 hom Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane
0.159 2.33E-20 YBL051C PIN4 hom Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage
0.155 1.61E-19 YJL129C TRK1 hom Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication
0.154 3.38E-19 YDL140C RPO21 het RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime
0.150 2.36E-18 YKL176C LST4 hom Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface
0.147 1.07E-17 YJL165C HAL5 hom Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication
0.144 6.90E-17 YFL024C EPL1 het Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb
0.143 8.33E-17 YDR466W PKH3 hom Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
0.129 5.16E-14 YDR467C_d YDR467C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.127 1.56E-13 YNL288W CAF40 hom Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p
0.124 5.34E-13 YGL011C SCL1 het Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
0.121 2.04E-12 YOL016C CMK2 hom Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication
0.119 3.96E-12 YCL021W-A_p YCL021W-A_p hom Putative protein of unknown function
0.119 5.53E-12 YBL082C ALG3 hom Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins
0.116 1.38E-11 YIL105C SLM1 hom Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication