YNL254C / RTC4

Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL254C / RTC4

Click on Significant Values for Screen Details ID:SGTC_331|Compound:k048-0133|FD-Score:-2.92|P-value:7.75E-4 ID:SGTC_332|Compound:4112-0506|FD-Score:-3.55|P-value:5.72E-5 ID:SGTC_366|Compound:0181-0206|FD-Score:3.17|P-value:2.30E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:3.55|P-value:4.43E-5 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:3.17|P-value:2.30E-4 ID:SGTC_591|Compound:4299-0174|FD-Score:-2.96|P-value:6.65E-4 ID:SGTC_618|Compound:k072-0232|FD-Score:4.37|P-value:7.38E-7 ID:SGTC_735|Compound:3076-0345|FD-Score:2.99|P-value:4.83E-4 ID:SGTC_739|Compound:1486-1183|FD-Score:4.02|P-value:4.76E-6 ID:SGTC_745|Compound:0469-0705|FD-Score:2.82|P-value:9.25E-4 ID:SGTC_780|Compound:0109-0199|FD-Score:3.74|P-value:1.84E-5 ID:SGTC_792|Compound:4534-1957|FD-Score:-4.16|P-value:3.05E-6 ID:SGTC_1115|Compound:galangin|FD-Score:2.89|P-value:7.13E-4 ID:SGTC_1142|Compound:3253-1408|FD-Score:4.13|P-value:2.66E-6 ID:SGTC_1169|Compound:aniline yellow|FD-Score:3.42|P-value:8.05E-5 ID:SGTC_1245|Compound:0418-0076|FD-Score:-2.98|P-value:6.27E-4 ID:SGTC_1287|Compound:0971-0001|FD-Score:-2.99|P-value:5.84E-4 ID:SGTC_1340|Compound:1486-1359|FD-Score:3.49|P-value:5.94E-5 ID:SGTC_1396|Compound:3511-0007|FD-Score:2.82|P-value:8.95E-4 ID:SGTC_1590|Compound:st077108|FD-Score:2.92|P-value:6.35E-4 ID:SGTC_1604|Compound:st002052|FD-Score:2.91|P-value:6.37E-4 ID:SGTC_1674|Compound:st016616|FD-Score:3.86|P-value:1.01E-5 ID:SGTC_1719|Compound:st032227|FD-Score:3.42|P-value:7.90E-5 ID:SGTC_1782|Compound:st039423|FD-Score:3.02|P-value:4.16E-4 ID:SGTC_1815|Compound:st051655|FD-Score:3.31|P-value:1.31E-4 ID:SGTC_1863|Compound:st057641|FD-Score:-3.16|P-value:3.09E-4 ID:SGTC_1931|Compound:st074438|FD-Score:-3.52|P-value:6.51E-5 ID:SGTC_1933|Compound:st074711|FD-Score:-4.10|P-value:4.20E-6 ID:SGTC_1937|Compound:st074714|FD-Score:-4.25|P-value:1.95E-6 ID:SGTC_2016|Compound:5121758|FD-Score:-3.79|P-value:1.89E-5 ID:SGTC_2265|Compound:7929201|FD-Score:-2.92|P-value:7.71E-4 ID:SGTC_2383|Compound:9036125|FD-Score:-4.10|P-value:4.15E-6 ID:SGTC_2386|Compound:9075165|FD-Score:-3.27|P-value:1.90E-4 ID:SGTC_2388|Compound:9080168|FD-Score:-3.46|P-value:8.48E-5 ID:SGTC_2575|Compound:plumbagin|FD-Score:-3.52|P-value:6.63E-5 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:-2.91|P-value:8.11E-4 ID:SGTC_2601|Compound:securinine|FD-Score:3.68|P-value:2.44E-5 ID:SGTC_2753|Compound:fluvastatin|FD-Score:4.21|P-value:1.73E-6 ID:SGTC_2785|Compound:7756463|FD-Score:-3.04|P-value:4.83E-4 ID:SGTC_2807|Compound:7945238|FD-Score:3.54|P-value:4.61E-5 ID:SGTC_2935|Compound:9038016|FD-Score:3.02|P-value:4.30E-4 ID:SGTC_3078|Compound:9116413|FD-Score:-3.11|P-value:3.75E-4 ID:SGTC_3083|Compound:9117465|FD-Score:5.14|P-value:7.90E-9 ID:SGTC_3088|Compound:9117578|FD-Score:-3.33|P-value:1.50E-4 ID:SGTC_3127|Compound:9126946|FD-Score:2.89|P-value:6.93E-4 ID:SGTC_3162|Compound:9099653|FD-Score:2.92|P-value:6.29E-4 ID:SGTC_3185|Compound:9108267|FD-Score:3.28|P-value:1.49E-4 ID:SGTC_3195|Compound:9113082|FD-Score:3.12|P-value:2.89E-4 ID:SGTC_331|Compound:k048-0133|FD-Score:-2.92|P-value:7.75E-4 ID:SGTC_332|Compound:4112-0506|FD-Score:-3.55|P-value:5.72E-5 ID:SGTC_366|Compound:0181-0206|FD-Score:3.17|P-value:2.30E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:3.55|P-value:4.43E-5 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:3.17|P-value:2.30E-4 ID:SGTC_591|Compound:4299-0174|FD-Score:-2.96|P-value:6.65E-4 ID:SGTC_618|Compound:k072-0232|FD-Score:4.37|P-value:7.38E-7 ID:SGTC_735|Compound:3076-0345|FD-Score:2.99|P-value:4.83E-4 ID:SGTC_739|Compound:1486-1183|FD-Score:4.02|P-value:4.76E-6 ID:SGTC_745|Compound:0469-0705|FD-Score:2.82|P-value:9.25E-4 ID:SGTC_780|Compound:0109-0199|FD-Score:3.74|P-value:1.84E-5 ID:SGTC_792|Compound:4534-1957|FD-Score:-4.16|P-value:3.05E-6 ID:SGTC_1115|Compound:galangin|FD-Score:2.89|P-value:7.13E-4 ID:SGTC_1142|Compound:3253-1408|FD-Score:4.13|P-value:2.66E-6 ID:SGTC_1169|Compound:aniline yellow|FD-Score:3.42|P-value:8.05E-5 ID:SGTC_1245|Compound:0418-0076|FD-Score:-2.98|P-value:6.27E-4 ID:SGTC_1287|Compound:0971-0001|FD-Score:-2.99|P-value:5.84E-4 ID:SGTC_1340|Compound:1486-1359|FD-Score:3.49|P-value:5.94E-5 ID:SGTC_1396|Compound:3511-0007|FD-Score:2.82|P-value:8.95E-4 ID:SGTC_1590|Compound:st077108|FD-Score:2.92|P-value:6.35E-4 ID:SGTC_1604|Compound:st002052|FD-Score:2.91|P-value:6.37E-4 ID:SGTC_1674|Compound:st016616|FD-Score:3.86|P-value:1.01E-5 ID:SGTC_1719|Compound:st032227|FD-Score:3.42|P-value:7.90E-5 ID:SGTC_1782|Compound:st039423|FD-Score:3.02|P-value:4.16E-4 ID:SGTC_1815|Compound:st051655|FD-Score:3.31|P-value:1.31E-4 ID:SGTC_1863|Compound:st057641|FD-Score:-3.16|P-value:3.09E-4 ID:SGTC_1931|Compound:st074438|FD-Score:-3.52|P-value:6.51E-5 ID:SGTC_1933|Compound:st074711|FD-Score:-4.10|P-value:4.20E-6 ID:SGTC_1937|Compound:st074714|FD-Score:-4.25|P-value:1.95E-6 ID:SGTC_2016|Compound:5121758|FD-Score:-3.79|P-value:1.89E-5 ID:SGTC_2265|Compound:7929201|FD-Score:-2.92|P-value:7.71E-4 ID:SGTC_2383|Compound:9036125|FD-Score:-4.10|P-value:4.15E-6 ID:SGTC_2386|Compound:9075165|FD-Score:-3.27|P-value:1.90E-4 ID:SGTC_2388|Compound:9080168|FD-Score:-3.46|P-value:8.48E-5 ID:SGTC_2575|Compound:plumbagin|FD-Score:-3.52|P-value:6.63E-5 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:-2.91|P-value:8.11E-4 ID:SGTC_2601|Compound:securinine|FD-Score:3.68|P-value:2.44E-5 ID:SGTC_2753|Compound:fluvastatin|FD-Score:4.21|P-value:1.73E-6 ID:SGTC_2785|Compound:7756463|FD-Score:-3.04|P-value:4.83E-4 ID:SGTC_2807|Compound:7945238|FD-Score:3.54|P-value:4.61E-5 ID:SGTC_2935|Compound:9038016|FD-Score:3.02|P-value:4.30E-4 ID:SGTC_3078|Compound:9116413|FD-Score:-3.11|P-value:3.75E-4 ID:SGTC_3083|Compound:9117465|FD-Score:5.14|P-value:7.90E-9 ID:SGTC_3088|Compound:9117578|FD-Score:-3.33|P-value:1.50E-4 ID:SGTC_3127|Compound:9126946|FD-Score:2.89|P-value:6.93E-4 ID:SGTC_3162|Compound:9099653|FD-Score:2.92|P-value:6.29E-4 ID:SGTC_3185|Compound:9108267|FD-Score:3.28|P-value:1.49E-4 ID:SGTC_3195|Compound:9113082|FD-Score:3.12|P-value:2.89E-4

Top fitness defect scores for YNL254C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3083 9117465 5.14 49.5 µM 7.90E-9 3 1.41E-7
2 SGTC_618 k072-0232 4.37 33.2 µM 7.38E-7 60S ribosome export 5 6.22E-6
3 SGTC_2753 fluvastatin 4.21 17.1 µM 1.73E-6 azole & statin 29 1.26E-5
4 SGTC_1142 3253-1408 4.13 9.8 µM 2.66E-6 6 1.81E-5
5 SGTC_739 1486-1183 4.02 203.0 µM 4.76E-6 15 2.95E-5
6 SGTC_1674 st016616 3.86 23.6 µM 1.01E-5 30 5.56E-5
7 SGTC_780 0109-0199 3.74 192.0 µM 1.84E-5 20 9.15E-5
8 SGTC_2601 securinine 3.68 100.0 µM 2.44E-5 35 1.16E-4
9 SGTC_372 0155-0182 3.55 15.9 µM 4.43E-5 mitochondrial response to ROS 42 1.91E-4
10 SGTC_2807 7945238 3.54 52.0 µM 4.61E-5 38 1.97E-4
11 SGTC_1340 1486-1359 3.49 257.0 µM 5.94E-5 40 2.44E-4
12 SGTC_1719 st032227 3.42 49.2 µM 7.90E-5 41 3.09E-4
13 SGTC_1169 aniline yellow 3.42 25.8 µM 8.05E-5 22 3.14E-4
14 SGTC_1815 st051655 3.31 58.2 µM 1.31E-4 TSC3-RPN4 19 4.73E-4
15 SGTC_3185 9108267 3.28 49.5 µM 1.49E-4 60S ribosome export 48 5.26E-4
16 SGTC_366 0181-0206 3.17 4.3 µM 2.30E-4 Golgi 80 7.58E-4
17 SGTC_546 peri-Naphthalide 3.17 147.0 µM 2.30E-4 DNA damage response 41 7.58E-4
18 SGTC_3195 9113082 3.12 49.5 µM 2.89E-4 40 9.16E-4
19 SGTC_1782 st039423 3.02 80.6 µM 4.16E-4 72 0.00124
20 SGTC_2935 9038016 3.02 30.0 µM 4.30E-4 61 0.00128

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.155 1.52E-19 YLR063W_p YLR063W_p hom Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene
0.112 8.89E-11 YOL043C NTG2 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair, localizes to the nucleus; sumoylated; NTG2 has a paralog, NTG1, that arose from the whole genome duplication
0.107 5.74E-10 YOR129C AFI1 hom Arf3p polarization-specific docking factor, required for the polarized distribution of the ADP-ribosylation factor, Arf3p; participates in polarity development and maintenance of a normal haploid budding pattern; interacts with Cnm7p
0.104 1.31E-9 YDL219W DTD1 hom D-Tyr-tRNA(Tyr) deacylase, functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes
0.090 1.78E-7 YOR016C ERP4 hom Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport
0.088 3.72E-7 YML075C HMG1 hom HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress
0.086 5.74E-7 YNL049C SFB2 hom Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb3p
0.085 9.46E-7 YPL103C FMP30 hom Mitochondrial inner membrane protein with a role in maintaining mitochondrial morphology and normal cardiolipin levels; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D
0.083 1.64E-6 YER033C ZRG8 hom Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency
0.080 3.51E-6 YLR390W-A CCW14 hom Covalently linked cell wall glycoprotein, present in the inner layer of the cell wall
0.079 4.18E-6 YOL082W ATG19 hom Receptor protein for the cytoplasm-to-vacuole targeting (Cvt) pathway; delivers cargo proteins aminopeptidase I (Ape1p) and alpha-mannosidase (Ams1p) to the phagophore assembly site for packaging into Cvt vesicles
0.076 9.16E-6 YGR088W CTT1 hom Cytosolic catalase T, has a role in protection from oxidative damage by hydrogen peroxide
0.076 1.06E-5 YJR046W TAH11 het DNA replication licensing factor, required for pre-replication complex assembly
0.075 1.36E-5 YAL016C-B_d YAL016C-B_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.074 1.67E-5 YER048C CAJ1 hom Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly