YNL255C / GIS2

Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL255C / GIS2

Click on Significant Values for Screen Details ID:SGTC_8|Compound:0354-0001|FD-Score:3.77|P-value:9.35E-5 ID:SGTC_64|Compound:000l-0018|FD-Score:4.69|P-value:1.73E-6 ID:SGTC_73|Compound:0327-0325|FD-Score:-3.17|P-value:9.20E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:4.02|P-value:3.43E-5 ID:SGTC_122|Compound:0236-0016|FD-Score:4.83|P-value:8.96E-7 ID:SGTC_173|Compound:1273-0061|FD-Score:-4.94|P-value:5.74E-7 ID:SGTC_198|Compound:3454-2873|FD-Score:3.67|P-value:1.38E-4 ID:SGTC_199|Compound:4063-0024|FD-Score:3.27|P-value:5.88E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:3.81|P-value:8.03E-5 ID:SGTC_531|Compound:3788-1495|FD-Score:3.33|P-value:4.83E-4 ID:SGTC_539|Compound:4859-0462|FD-Score:4.37|P-value:7.45E-6 ID:SGTC_841|Compound:0251-0215|FD-Score:-3.87|P-value:7.11E-5 ID:SGTC_853|Compound:0419-0306|FD-Score:3.13|P-value:9.62E-4 ID:SGTC_953|Compound:1188-0045|FD-Score:3.50|P-value:2.59E-4 ID:SGTC_968|Compound:1309-0633|FD-Score:3.78|P-value:9.14E-5 ID:SGTC_1252|Compound:0493-0875|FD-Score:6.06|P-value:1.03E-9 ID:SGTC_1326|Compound:1431-2094|FD-Score:3.70|P-value:1.25E-4 ID:SGTC_1334|Compound:1486-0610|FD-Score:4.04|P-value:3.11E-5 ID:SGTC_1365|Compound:1672-3266|FD-Score:4.21|P-value:1.53E-5 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.91|P-value:5.43E-5 ID:SGTC_1378|Compound:2093-0007|FD-Score:-3.34|P-value:5.10E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:-3.58|P-value:2.16E-4 ID:SGTC_1577|Compound:3(2'-chlorophenyl)-7-ethoxycoumarin|FD-Score:3.22|P-value:7.01E-4 ID:SGTC_1620|Compound:st003704|FD-Score:3.20|P-value:7.49E-4 ID:SGTC_1682|Compound:st012968|FD-Score:3.23|P-value:6.84E-4 ID:SGTC_1745|Compound:st081495|FD-Score:9.56|P-value:1.82E-21 ID:SGTC_1747|Compound:st037496|FD-Score:-3.57|P-value:2.26E-4 ID:SGTC_1786|Compound:berberine|FD-Score:3.37|P-value:4.15E-4 ID:SGTC_1794|Compound:st048918|FD-Score:-3.41|P-value:4.10E-4 ID:SGTC_1876|Compound:myriocin|FD-Score:-3.33|P-value:5.28E-4 ID:SGTC_1927|Compound:st060202|FD-Score:-3.58|P-value:2.18E-4 ID:SGTC_1948|Compound:st076329|FD-Score:5.43|P-value:3.92E-8 ID:SGTC_1950|Compound:st077106|FD-Score:7.84|P-value:4.78E-15 ID:SGTC_2009|Compound:4012297|FD-Score:3.67|P-value:1.39E-4 ID:SGTC_2012|Compound:4023501|FD-Score:-4.48|P-value:5.35E-6 ID:SGTC_2013|Compound:4024628|FD-Score:3.77|P-value:9.55E-5 ID:SGTC_2052|Compound:5265182|FD-Score:3.14|P-value:9.20E-4 ID:SGTC_2116|Compound:5511452|FD-Score:3.81|P-value:8.08E-5 ID:SGTC_2136|Compound:5328288|FD-Score:3.22|P-value:7.05E-4 ID:SGTC_2172|Compound:5754775|FD-Score:3.84|P-value:7.06E-5 ID:SGTC_2186|Compound:6124449|FD-Score:4.16|P-value:1.91E-5 ID:SGTC_2297|Compound:7971671|FD-Score:3.90|P-value:5.68E-5 ID:SGTC_2317|Compound:5986382|FD-Score:3.12|P-value:9.89E-4 ID:SGTC_2320|Compound:7373003|FD-Score:-3.73|P-value:1.22E-4 ID:SGTC_2383|Compound:9036125|FD-Score:-3.46|P-value:3.43E-4 ID:SGTC_2432|Compound:5630864|FD-Score:3.40|P-value:3.83E-4 ID:SGTC_2557|Compound:derrustone|FD-Score:5.64|P-value:1.24E-8 ID:SGTC_2559|Compound:tetrahydrosappanone a trimethyl ether|FD-Score:-3.94|P-value:5.40E-5 ID:SGTC_2560|Compound:caryophyllene|FD-Score:3.43|P-value:3.35E-4 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:4.32|P-value:9.60E-6 ID:SGTC_2610|Compound:ononetin|FD-Score:3.51|P-value:2.53E-4 ID:SGTC_2817|Compound:7995342|FD-Score:-4.32|P-value:1.10E-5 ID:SGTC_2818|Compound:7996756|FD-Score:-3.67|P-value:1.57E-4 ID:SGTC_2922|Compound:7999257|FD-Score:-3.83|P-value:8.39E-5 ID:SGTC_2932|Compound:9016711|FD-Score:-3.44|P-value:3.69E-4 ID:SGTC_2934|Compound:9036370|FD-Score:-3.77|P-value:1.07E-4 ID:SGTC_2937|Compound:9044753|FD-Score:4.20|P-value:1.59E-5 ID:SGTC_2939|Compound:9044578|FD-Score:-4.33|P-value:1.04E-5 ID:SGTC_2940|Compound:9060638|FD-Score:3.14|P-value:9.29E-4 ID:SGTC_2967|Compound:9089538|FD-Score:3.31|P-value:5.13E-4 ID:SGTC_2970|Compound:9090938|FD-Score:3.80|P-value:8.43E-5 ID:SGTC_3189|Compound:9110682|FD-Score:-3.16|P-value:9.43E-4 ID:SGTC_3200|Compound:9114385|FD-Score:-3.97|P-value:4.84E-5 ID:SGTC_3204|Compound:9114183|FD-Score:3.86|P-value:6.56E-5 ID:SGTC_8|Compound:0354-0001|FD-Score:3.77|P-value:9.35E-5 ID:SGTC_64|Compound:000l-0018|FD-Score:4.69|P-value:1.73E-6 ID:SGTC_73|Compound:0327-0325|FD-Score:-3.17|P-value:9.20E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:4.02|P-value:3.43E-5 ID:SGTC_122|Compound:0236-0016|FD-Score:4.83|P-value:8.96E-7 ID:SGTC_173|Compound:1273-0061|FD-Score:-4.94|P-value:5.74E-7 ID:SGTC_198|Compound:3454-2873|FD-Score:3.67|P-value:1.38E-4 ID:SGTC_199|Compound:4063-0024|FD-Score:3.27|P-value:5.88E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:3.81|P-value:8.03E-5 ID:SGTC_531|Compound:3788-1495|FD-Score:3.33|P-value:4.83E-4 ID:SGTC_539|Compound:4859-0462|FD-Score:4.37|P-value:7.45E-6 ID:SGTC_841|Compound:0251-0215|FD-Score:-3.87|P-value:7.11E-5 ID:SGTC_853|Compound:0419-0306|FD-Score:3.13|P-value:9.62E-4 ID:SGTC_953|Compound:1188-0045|FD-Score:3.50|P-value:2.59E-4 ID:SGTC_968|Compound:1309-0633|FD-Score:3.78|P-value:9.14E-5 ID:SGTC_1252|Compound:0493-0875|FD-Score:6.06|P-value:1.03E-9 ID:SGTC_1326|Compound:1431-2094|FD-Score:3.70|P-value:1.25E-4 ID:SGTC_1334|Compound:1486-0610|FD-Score:4.04|P-value:3.11E-5 ID:SGTC_1365|Compound:1672-3266|FD-Score:4.21|P-value:1.53E-5 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.91|P-value:5.43E-5 ID:SGTC_1378|Compound:2093-0007|FD-Score:-3.34|P-value:5.10E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:-3.58|P-value:2.16E-4 ID:SGTC_1577|Compound:3(2'-chlorophenyl)-7-ethoxycoumarin|FD-Score:3.22|P-value:7.01E-4 ID:SGTC_1620|Compound:st003704|FD-Score:3.20|P-value:7.49E-4 ID:SGTC_1682|Compound:st012968|FD-Score:3.23|P-value:6.84E-4 ID:SGTC_1745|Compound:st081495|FD-Score:9.56|P-value:1.82E-21 ID:SGTC_1747|Compound:st037496|FD-Score:-3.57|P-value:2.26E-4 ID:SGTC_1786|Compound:berberine|FD-Score:3.37|P-value:4.15E-4 ID:SGTC_1794|Compound:st048918|FD-Score:-3.41|P-value:4.10E-4 ID:SGTC_1876|Compound:myriocin|FD-Score:-3.33|P-value:5.28E-4 ID:SGTC_1927|Compound:st060202|FD-Score:-3.58|P-value:2.18E-4 ID:SGTC_1948|Compound:st076329|FD-Score:5.43|P-value:3.92E-8 ID:SGTC_1950|Compound:st077106|FD-Score:7.84|P-value:4.78E-15 ID:SGTC_2009|Compound:4012297|FD-Score:3.67|P-value:1.39E-4 ID:SGTC_2012|Compound:4023501|FD-Score:-4.48|P-value:5.35E-6 ID:SGTC_2013|Compound:4024628|FD-Score:3.77|P-value:9.55E-5 ID:SGTC_2052|Compound:5265182|FD-Score:3.14|P-value:9.20E-4 ID:SGTC_2116|Compound:5511452|FD-Score:3.81|P-value:8.08E-5 ID:SGTC_2136|Compound:5328288|FD-Score:3.22|P-value:7.05E-4 ID:SGTC_2172|Compound:5754775|FD-Score:3.84|P-value:7.06E-5 ID:SGTC_2186|Compound:6124449|FD-Score:4.16|P-value:1.91E-5 ID:SGTC_2297|Compound:7971671|FD-Score:3.90|P-value:5.68E-5 ID:SGTC_2317|Compound:5986382|FD-Score:3.12|P-value:9.89E-4 ID:SGTC_2320|Compound:7373003|FD-Score:-3.73|P-value:1.22E-4 ID:SGTC_2383|Compound:9036125|FD-Score:-3.46|P-value:3.43E-4 ID:SGTC_2432|Compound:5630864|FD-Score:3.40|P-value:3.83E-4 ID:SGTC_2557|Compound:derrustone|FD-Score:5.64|P-value:1.24E-8 ID:SGTC_2559|Compound:tetrahydrosappanone a trimethyl ether|FD-Score:-3.94|P-value:5.40E-5 ID:SGTC_2560|Compound:caryophyllene|FD-Score:3.43|P-value:3.35E-4 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:4.32|P-value:9.60E-6 ID:SGTC_2610|Compound:ononetin|FD-Score:3.51|P-value:2.53E-4 ID:SGTC_2817|Compound:7995342|FD-Score:-4.32|P-value:1.10E-5 ID:SGTC_2818|Compound:7996756|FD-Score:-3.67|P-value:1.57E-4 ID:SGTC_2922|Compound:7999257|FD-Score:-3.83|P-value:8.39E-5 ID:SGTC_2932|Compound:9016711|FD-Score:-3.44|P-value:3.69E-4 ID:SGTC_2934|Compound:9036370|FD-Score:-3.77|P-value:1.07E-4 ID:SGTC_2937|Compound:9044753|FD-Score:4.20|P-value:1.59E-5 ID:SGTC_2939|Compound:9044578|FD-Score:-4.33|P-value:1.04E-5 ID:SGTC_2940|Compound:9060638|FD-Score:3.14|P-value:9.29E-4 ID:SGTC_2967|Compound:9089538|FD-Score:3.31|P-value:5.13E-4 ID:SGTC_2970|Compound:9090938|FD-Score:3.80|P-value:8.43E-5 ID:SGTC_3189|Compound:9110682|FD-Score:-3.16|P-value:9.43E-4 ID:SGTC_3200|Compound:9114385|FD-Score:-3.97|P-value:4.84E-5 ID:SGTC_3204|Compound:9114183|FD-Score:3.86|P-value:6.56E-5

Top fitness defect scores for YNL255C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1745 st081495 9.56 74.0 µM 1.82E-21 RNA processing & uracil transport 7 6.11E-22
2 SGTC_1950 st077106 7.84 7.2 µM 4.78E-15 RNA processing & uracil transport 3 2.33E-15
3 SGTC_1252 0493-0875 6.06 34.8 µM 1.03E-9 3 6.79E-10
4 SGTC_2557 derrustone 5.64 83.8 µM 1.24E-8 9 8.68E-9
5 SGTC_1948 st076329 5.43 66.6 µM 3.92E-8 7 2.82E-8
6 SGTC_122 0236-0016 4.83 402.4 µM 8.96E-7 RNA processing & uracil transport 19 6.96E-7
7 SGTC_64 000l-0018 4.69 272.1 µM 1.73E-6 ubiquinone biosynthesis & proteasome 118 1.37E-6
8 SGTC_539 4859-0462 4.37 2.5 µM 7.45E-6 RNA pol III & RNase P/MRP 14 6.08E-6
9 SGTC_2609 2,3,4-trihydroxy-4'-ethoxybenzophenone 4.32 70.0 µM 9.60E-6 redox potentiating 64 7.88E-6
10 SGTC_1365 1672-3266 4.21 55.4 µM 1.53E-5 14 1.27E-5
11 SGTC_2937 9044753 4.20 11.3 µM 1.59E-5 60S ribosome export 29 1.32E-5
12 SGTC_2186 6124449 4.16 200.0 µM 1.91E-5 RSC complex & mRNA processing 15 1.59E-5
13 SGTC_1334 1486-0610 4.04 236.0 µM 3.11E-5 60S ribosome export 15 2.62E-5
14 SGTC_86 0417-1630 4.02 471.9 µM 3.43E-5 ubiquinone biosynthesis & proteasome 147 2.90E-5
15 SGTC_1371 2421-0008 3.91 32.7 µM 5.43E-5 mitochondrial processes 42 4.64E-5
16 SGTC_2297 7971671 3.90 152.3 µM 5.68E-5 RNA processing & uracil transport 26 4.86E-5
17 SGTC_3204 9114183 3.86 49.5 µM 6.56E-5 37 5.62E-5
18 SGTC_2172 5754775 3.84 163.9 µM 7.06E-5 16 6.07E-5
19 SGTC_339 3091-4833 3.81 71.4 µM 8.03E-5 iron homeostasis 24 6.91E-5
20 SGTC_2116 5511452 3.81 16.9 µM 8.08E-5 16 6.96E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.129 7.51E-14 YLR015W BRE2 hom Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L
0.121 1.79E-12 YCL038C ATG22 hom Vacuolar integral membrane protein required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation
0.114 2.98E-11 YLR420W URA4 hom Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate
0.111 9.77E-11 YJR002W MPP10 het Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p
0.108 3.02E-10 YDR290W_d YDR290W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103
0.105 9.20E-10 YMR314W PRE5 het Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
0.105 1.09E-9 YGR090W UTP22 het Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals
0.102 2.68E-9 YML106W URA5 hom Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication
0.096 2.29E-8 YLR014C PPR1 hom Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p
0.095 3.12E-8 YDR289C RTT103 hom Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition
0.094 4.72E-8 YER094C PUP3 het Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10
0.094 4.73E-8 YHL016C DUR3 hom Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway
0.091 1.39E-7 YNL111C CYB5 hom Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation
0.090 1.77E-7 YLR418C CDC73 hom Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress
0.090 1.81E-7 YDR469W SDC1 hom Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30