YNL260C / LTO1

Essential protein that forms a complex with Rli1p and Yae1p; ortholog of human ORAOV1, which is overexpressed in solid tumors; inviability of null mutant under standard conditions is complemented by overexpression of ORAOV1; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species

Zygosity: Heterozygous strain
fixedexpanded
Profile for YNL260C / LTO1

Click on Significant Values for Screen Details

Top fitness defect scores for YNL260C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_402 epigallocatechin gallate 6.73 10.0 µM 2.60E-15 1 8.40E-12 1.76
2 SGTC_2318 6029776 6.00 164.0 µM 1.45E-12 2 9.65E-10 2.14
3 SGTC_2398 5228213 4.45 48.8 µM 1.03E-7 iron homeostasis 12 4.22E-6 0.53
4 SGTC_2275 7947463 3.92 200.0 µM 2.40E-6 iron homeostasis 12 4.51E-5 0.03
5 SGTC_394 menadione 3.91 8.3 µM 2.43E-6 superoxide 6 4.55E-5 0.16
6 SGTC_2158 5657123 3.76 3.6 µM 5.70E-6 14 8.66E-5 0.01
7 SGTC_2625 euphol 3.71 100.0 µM 7.14E-6 11 1.03E-4 0.23
8 SGTC_269 exalamide 3.69 29.0 µM 7.97E-6 ergosterol biosynthesis 6 1.11E-4 0.08
9 SGTC_398 bronopol 3.59 33.3 µM 1.36E-5 RNA processing & uracil transport 18 1.66E-4 0.04
10 SGTC_1251 0490-5699 3.39 265.0 µM 3.60E-5 11 3.47E-4 0.04
11 SGTC_807 1315-0151 3.30 48.0 µM 5.61E-5 15 4.85E-4 0.02
12 SGTC_3332 9141069 3.24 11.2 µM 7.50E-5 6 6.03E-4 0.08
13 SGTC_650 1328-0195 3.20 38.0 µM 8.81E-5 7 6.81E-4 8.56E-4
14 SGTC_2395 5646831 3.11 180.2 µM 1.34E-4 8 9.36E-4 0.04
15 SGTC_420 mitomycin C 3.11 81.9 mM 1.35E-4 13 9.39E-4 0.07
16 SGTC_1748 st037455 3.06 53.6 µM 1.66E-4 17 0.00110 0.01
17 SGTC_987 1497-1013 3.02 404.0 µM 1.98E-4 10 0.00126 0.05
18 SGTC_36 0335-0881 3.00 14.2 µM 2.22E-4 ubiquinone biosynthesis & proteasome 53 0.00137 0.06
19 SGTC_33 0986-0033 2.91 35.8 µM 3.17E-4 calcium & mitochondrial duress 8 0.00179 0.04
20 SGTC_2444 5485484 2.90 36.4 µM 3.37E-4 26 0.00188 0.05

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.188 3.80E-28 YKL072W STB6 hom Protein that binds Sin3p in a two-hybrid assay
0.156 9.21E-20 YOR087W YVC1 hom Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock
0.130 3.97E-14 YER065C ICL1 hom Isocitrate lyase, catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose
0.130 5.14E-14 YMR111C YMR111C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene; forms nuclear foci upon DNA replication stress
0.126 2.07E-13 YNL076W MKS1 hom Pleiotropic negative transcriptional regulator involved in Ras-CAMP and lysine biosynthetic pathways and nitrogen regulation; involved in retrograde (RTG) mitochondria-to-nucleus signaling
0.125 3.85E-13 YDR180W SCC2 het Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin
0.122 1.39E-12 YPL064C CWC27 hom Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress
0.121 2.21E-12 YLR307C-A_p YLR307C-A_p hom Putative protein of unknown function
0.121 2.19E-12 YLR365W_d YLR365W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps dubious gene YLR364C-A; YLR365W is not an essential gene
0.106 7.04E-10 YGR185C TYS1 het Cytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; protein abundance increases in response to DNA replication stress
0.105 9.21E-10 YKL131W_d YKL131W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.101 4.65E-9 YLR363W-A YLR363W-A hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; relocalizes from nucleus to nucleolus upon DNA replication stress
0.099 7.67E-9 YDR441C APT2 hom Potential adenine phosphoribosyltransferase; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity; APT2 has a paralog, APT1, that arose from the whole genome duplication
0.099 9.95E-9 YPR010C RPA135 het RNA polymerase I second largest subunit A135
0.098 1.49E-8 YLR290C_p YLR290C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR290C is not an essential gene