YNL265C / IST1

Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL265C / IST1

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:-3.52|P-value:2.93E-4 ID:SGTC_77|Compound:0828-0286|FD-Score:-3.54|P-value:2.70E-4 ID:SGTC_285|Compound:1486-1325|FD-Score:3.31|P-value:6.89E-4 ID:SGTC_292|Compound:k038-0015|FD-Score:-3.23|P-value:7.98E-4 ID:SGTC_298|Compound:1391-0729|FD-Score:4.62|P-value:3.96E-6 ID:SGTC_307|Compound:1501-1223|FD-Score:-4.50|P-value:5.50E-6 ID:SGTC_309|Compound:k408-0051|FD-Score:-3.69|P-value:1.57E-4 ID:SGTC_424|Compound:splitomicin|FD-Score:3.50|P-value:3.60E-4 ID:SGTC_437|Compound:s-farnesyl-l-cysteine methyl ester|FD-Score:4.11|P-value:3.50E-5 ID:SGTC_464|Compound:bw-b 70c|FD-Score:3.32|P-value:6.72E-4 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:3.32|P-value:6.66E-4 ID:SGTC_579|Compound:4491-1009|FD-Score:3.38|P-value:5.48E-4 ID:SGTC_786|Compound:4544-0069|FD-Score:3.94|P-value:6.99E-5 ID:SGTC_964|Compound:1287-0107|FD-Score:-3.53|P-value:2.81E-4 ID:SGTC_1021|Compound:4476-0760|FD-Score:3.57|P-value:2.86E-4 ID:SGTC_1075|Compound:aripiprazole|FD-Score:3.92|P-value:7.59E-5 ID:SGTC_1105|Compound:epirubicin|FD-Score:-3.23|P-value:7.82E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.37|P-value:5.74E-4 ID:SGTC_1173|Compound:0443-0020|FD-Score:3.52|P-value:3.35E-4 ID:SGTC_1318|Compound:1280-3875|FD-Score:3.72|P-value:1.62E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:4.29|P-value:1.69E-5 ID:SGTC_1484|Compound:3473-2379|FD-Score:3.83|P-value:1.09E-4 ID:SGTC_1514|Compound:1189-1591|FD-Score:4.95|P-value:8.58E-7 ID:SGTC_1761|Compound:st044296|FD-Score:3.78|P-value:1.33E-4 ID:SGTC_2260|Compound:7964312|FD-Score:3.38|P-value:5.48E-4 ID:SGTC_2444|Compound:5485484|FD-Score:-3.34|P-value:5.44E-4 ID:SGTC_2445|Compound:5742597|FD-Score:-4.61|P-value:3.44E-6 ID:SGTC_2447|Compound:5741742|FD-Score:5.36|P-value:1.06E-7 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:3.36|P-value:5.86E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:-4.50|P-value:5.62E-6 ID:SGTC_2769|Compound:toremifene|FD-Score:3.94|P-value:7.07E-5 ID:SGTC_2794|Compound:7785382|FD-Score:4.12|P-value:3.39E-5 ID:SGTC_2833|Compound:9001315|FD-Score:-5.69|P-value:1.44E-8 ID:SGTC_2856|Compound:9022925|FD-Score:-4.03|P-value:4.15E-5 ID:SGTC_2953|Compound:9074162|FD-Score:3.68|P-value:1.92E-4 ID:SGTC_2988|Compound:9073640|FD-Score:5.30|P-value:1.49E-7 ID:SGTC_3026|Compound:9090633|FD-Score:4.34|P-value:1.37E-5 ID:SGTC_3028|Compound:9087778|FD-Score:3.78|P-value:1.29E-4 ID:SGTC_3038|Compound:9091290|FD-Score:4.42|P-value:9.79E-6 ID:SGTC_3104|Compound:9120930|FD-Score:3.43|P-value:4.68E-4 ID:SGTC_3128|Compound:9127691|FD-Score:4.54|P-value:5.71E-6 ID:SGTC_3147|Compound:9097027|FD-Score:8.83|P-value:5.71E-18 ID:SGTC_3150|Compound:9097574|FD-Score:-3.26|P-value:7.07E-4 ID:SGTC_3154|Compound:9100113|FD-Score:-4.22|P-value:1.89E-5 ID:SGTC_3160|Compound:9100216|FD-Score:3.33|P-value:6.51E-4 ID:SGTC_3170|Compound:9103054|FD-Score:3.68|P-value:1.92E-4 ID:SGTC_3188|Compound:9110789|FD-Score:3.61|P-value:2.42E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.52|P-value:2.93E-4 ID:SGTC_77|Compound:0828-0286|FD-Score:-3.54|P-value:2.70E-4 ID:SGTC_285|Compound:1486-1325|FD-Score:3.31|P-value:6.89E-4 ID:SGTC_292|Compound:k038-0015|FD-Score:-3.23|P-value:7.98E-4 ID:SGTC_298|Compound:1391-0729|FD-Score:4.62|P-value:3.96E-6 ID:SGTC_307|Compound:1501-1223|FD-Score:-4.50|P-value:5.50E-6 ID:SGTC_309|Compound:k408-0051|FD-Score:-3.69|P-value:1.57E-4 ID:SGTC_424|Compound:splitomicin|FD-Score:3.50|P-value:3.60E-4 ID:SGTC_437|Compound:s-farnesyl-l-cysteine methyl ester|FD-Score:4.11|P-value:3.50E-5 ID:SGTC_464|Compound:bw-b 70c|FD-Score:3.32|P-value:6.72E-4 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:3.32|P-value:6.66E-4 ID:SGTC_579|Compound:4491-1009|FD-Score:3.38|P-value:5.48E-4 ID:SGTC_786|Compound:4544-0069|FD-Score:3.94|P-value:6.99E-5 ID:SGTC_964|Compound:1287-0107|FD-Score:-3.53|P-value:2.81E-4 ID:SGTC_1021|Compound:4476-0760|FD-Score:3.57|P-value:2.86E-4 ID:SGTC_1075|Compound:aripiprazole|FD-Score:3.92|P-value:7.59E-5 ID:SGTC_1105|Compound:epirubicin|FD-Score:-3.23|P-value:7.82E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.37|P-value:5.74E-4 ID:SGTC_1173|Compound:0443-0020|FD-Score:3.52|P-value:3.35E-4 ID:SGTC_1318|Compound:1280-3875|FD-Score:3.72|P-value:1.62E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:4.29|P-value:1.69E-5 ID:SGTC_1484|Compound:3473-2379|FD-Score:3.83|P-value:1.09E-4 ID:SGTC_1514|Compound:1189-1591|FD-Score:4.95|P-value:8.58E-7 ID:SGTC_1761|Compound:st044296|FD-Score:3.78|P-value:1.33E-4 ID:SGTC_2260|Compound:7964312|FD-Score:3.38|P-value:5.48E-4 ID:SGTC_2444|Compound:5485484|FD-Score:-3.34|P-value:5.44E-4 ID:SGTC_2445|Compound:5742597|FD-Score:-4.61|P-value:3.44E-6 ID:SGTC_2447|Compound:5741742|FD-Score:5.36|P-value:1.06E-7 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:3.36|P-value:5.86E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:-4.50|P-value:5.62E-6 ID:SGTC_2769|Compound:toremifene|FD-Score:3.94|P-value:7.07E-5 ID:SGTC_2794|Compound:7785382|FD-Score:4.12|P-value:3.39E-5 ID:SGTC_2833|Compound:9001315|FD-Score:-5.69|P-value:1.44E-8 ID:SGTC_2856|Compound:9022925|FD-Score:-4.03|P-value:4.15E-5 ID:SGTC_2953|Compound:9074162|FD-Score:3.68|P-value:1.92E-4 ID:SGTC_2988|Compound:9073640|FD-Score:5.30|P-value:1.49E-7 ID:SGTC_3026|Compound:9090633|FD-Score:4.34|P-value:1.37E-5 ID:SGTC_3028|Compound:9087778|FD-Score:3.78|P-value:1.29E-4 ID:SGTC_3038|Compound:9091290|FD-Score:4.42|P-value:9.79E-6 ID:SGTC_3104|Compound:9120930|FD-Score:3.43|P-value:4.68E-4 ID:SGTC_3128|Compound:9127691|FD-Score:4.54|P-value:5.71E-6 ID:SGTC_3147|Compound:9097027|FD-Score:8.83|P-value:5.71E-18 ID:SGTC_3150|Compound:9097574|FD-Score:-3.26|P-value:7.07E-4 ID:SGTC_3154|Compound:9100113|FD-Score:-4.22|P-value:1.89E-5 ID:SGTC_3160|Compound:9100216|FD-Score:3.33|P-value:6.51E-4 ID:SGTC_3170|Compound:9103054|FD-Score:3.68|P-value:1.92E-4 ID:SGTC_3188|Compound:9110789|FD-Score:3.61|P-value:2.42E-4

Top fitness defect scores for YNL265C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3147 9097027 8.83 49.5 µM 5.71E-18 1 5.18E-19
2 SGTC_2447 5741742 5.36 33.8 µM 1.06E-7 7 4.10E-8
3 SGTC_2988 9073640 5.30 71.4 µM 1.49E-7 9 5.91E-8
4 SGTC_1514 1189-1591 4.95 158.0 µM 8.58E-7 21 3.79E-7
5 SGTC_298 1391-0729 4.62 10.0 µM 3.96E-6 calcium & mitochondrial duress 12 1.92E-6
6 SGTC_3128 9127691 4.54 49.5 µM 5.71E-6 12 2.83E-6
7 SGTC_3038 9091290 4.42 49.5 µM 9.79E-6 9 5.02E-6
8 SGTC_3026 9090633 4.34 49.5 µM 1.37E-5 12 7.16E-6
9 SGTC_1468 k284-1086 4.29 134.0 µM 1.69E-5 DNA intercalators 26 8.97E-6
10 SGTC_2794 7785382 4.12 27.3 µM 3.39E-5 30 1.88E-5
11 SGTC_437 s-farnesyl-l-cysteine methyl ester 4.11 6.7 µM 3.50E-5 11 1.94E-5
12 SGTC_786 4544-0069 3.94 423.0 µM 6.99E-5 28 4.05E-5
13 SGTC_2769 toremifene 3.94 3.7 µM 7.07E-5 23 4.10E-5
14 SGTC_1075 aripiprazole 3.92 15.2 µM 7.59E-5 31 4.42E-5
15 SGTC_1484 3473-2379 3.83 286.0 µM 1.09E-4 19 6.46E-5
16 SGTC_3028 9087778 3.78 49.5 µM 1.29E-4 18 7.78E-5
17 SGTC_1761 st044296 3.78 37.0 µM 1.33E-4 18 7.98E-5
18 SGTC_1318 1280-3875 3.72 124.0 µM 1.62E-4 32 9.85E-5
19 SGTC_3170 9103054 3.68 49.5 µM 1.92E-4 60S ribosome export 19 1.18E-4
20 SGTC_2953 9074162 3.68 66.5 µM 1.92E-4 35 1.18E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.103 2.18E-9 YMR132C_p JLP2_p hom Protein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein)
0.081 2.36E-6 YPR195C_d YPR195C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.081 2.52E-6 YOR361C PRT1 het eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes
0.081 2.80E-6 YFL029C CAK1 het Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases
0.080 3.39E-6 YML070W DAK1 hom Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation
0.074 1.87E-5 YFR031C-A RPL2A hom Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication
0.073 2.12E-5 YER180C ISC10 hom Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells
0.073 2.16E-5 YDR243C PRP28 het RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site
0.073 2.34E-5 YGL039W YGL039W hom Oxidoreductase shown to reduce carbonyl compounds to chiral alcohols
0.073 2.35E-5 YLL013C PUF3 hom Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins
0.072 2.78E-5 YER057C HMF1 hom Member of the p14.5 protein family with similarity to Mmf1p, functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro
0.072 2.90E-5 YLR070C XYL2 hom Xylitol dehydrogenase, converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect
0.072 3.17E-5 YBR168W PEX32 hom Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p
0.072 3.38E-5 YOR084W LPX1 hom Oleic acid-inducible, peroxisomal matrix localized lipase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; peroxisomal import is dependent on the PTS1 receptor, Pex5p and on self-interaction
0.071 3.62E-5 YOL100W PKH2 hom Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p