YNL272C / SEC2

Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles

Zygosity: Heterozygous strain
fixedexpanded
Profile for YNL272C / SEC2

Click on Significant Values for Screen Details ID:SGTC_41|Compound:0478-0632|FD-Score:2.97|P-value:9.10E-4 ID:SGTC_57|Compound:miconazole|FD-Score:3.46|P-value:1.41E-4 ID:SGTC_252|Compound:miconazole|FD-Score:3.51|P-value:1.15E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:5.17|P-value:2.73E-8 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:-3.99|P-value:1.36E-5 ID:SGTC_393|Compound:acivicin|FD-Score:-3.51|P-value:1.09E-4 ID:SGTC_394|Compound:menadione|FD-Score:4.07|P-value:9.69E-6 ID:SGTC_620|Compound:0109-0052|FD-Score:-2.98|P-value:8.42E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:3.14|P-value:4.83E-4 ID:SGTC_622|Compound:1348-1566|FD-Score:5.27|P-value:1.55E-8 ID:SGTC_637|Compound:1181-0519|FD-Score:8.17|P-value:4.68E-18 ID:SGTC_646|Compound:4092-0839|FD-Score:3.61|P-value:7.63E-5 ID:SGTC_834|Compound:0312-0008|FD-Score:3.19|P-value:3.99E-4 ID:SGTC_838|Compound:0109-0203|FD-Score:3.24|P-value:3.39E-4 ID:SGTC_918|Compound:glabranin|FD-Score:3.22|P-value:3.62E-4 ID:SGTC_961|Compound:1252-1571|FD-Score:3.91|P-value:2.02E-5 ID:SGTC_1126|Compound:isoteolin|FD-Score:2.97|P-value:9.19E-4 ID:SGTC_1170|Compound:0310-0127|FD-Score:3.00|P-value:8.16E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:-3.95|P-value:1.64E-5 ID:SGTC_1252|Compound:0493-0875|FD-Score:-2.96|P-value:9.15E-4 ID:SGTC_1260|Compound:0700-1617|FD-Score:-3.12|P-value:5.10E-4 ID:SGTC_1331|Compound:1472-0049|FD-Score:-2.98|P-value:8.49E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:3.46|P-value:1.37E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:3.46|P-value:1.41E-4 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:3.45|P-value:1.47E-4 ID:SGTC_1754|Compound:st042065|FD-Score:3.13|P-value:4.99E-4 ID:SGTC_1850|Compound:st056238|FD-Score:4.93|P-value:1.12E-7 ID:SGTC_1864|Compound:st057645|FD-Score:3.27|P-value:3.04E-4 ID:SGTC_2024|Compound:5144227|FD-Score:3.57|P-value:9.06E-5 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.34|P-value:2.27E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:-4.20|P-value:4.95E-6 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-7.57|P-value:8.48E-16 ID:SGTC_2413|Compound:inermin|FD-Score:3.11|P-value:5.56E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-4.21|P-value:4.68E-6 ID:SGTC_2445|Compound:5742597|FD-Score:5.86|P-value:3.60E-10 ID:SGTC_2452|Compound:5863136|FD-Score:3.00|P-value:8.12E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:3.51|P-value:1.14E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:3.07|P-value:6.23E-4 ID:SGTC_2549|Compound:cedrol|FD-Score:-3.12|P-value:5.02E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:4.40|P-value:1.94E-6 ID:SGTC_2594|Compound:a-mangostin|FD-Score:-3.79|P-value:3.32E-5 ID:SGTC_2596|Compound:sinensetin|FD-Score:3.10|P-value:5.75E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:-3.78|P-value:3.53E-5 ID:SGTC_2791|Compound:5663188|FD-Score:3.26|P-value:3.13E-4 ID:SGTC_2800|Compound:7887935|FD-Score:-3.20|P-value:3.73E-4 ID:SGTC_2835|Compound:9006588|FD-Score:6.57|P-value:2.62E-12 ID:SGTC_2850|Compound:9015429|FD-Score:3.37|P-value:2.02E-4 ID:SGTC_2873|Compound:9040596|FD-Score:3.77|P-value:3.83E-5 ID:SGTC_2884|Compound:9058502|FD-Score:3.12|P-value:5.27E-4 ID:SGTC_2951|Compound:9072950|FD-Score:3.75|P-value:4.17E-5 ID:SGTC_3118|Compound:9124250|FD-Score:2.98|P-value:8.91E-4 ID:SGTC_3150|Compound:9097574|FD-Score:-3.74|P-value:4.13E-5 ID:SGTC_3151|Compound:9099821|FD-Score:-3.77|P-value:3.62E-5 ID:SGTC_3152|Compound:9099253|FD-Score:4.19|P-value:5.37E-6 ID:SGTC_3308|Compound:9128332|FD-Score:4.25|P-value:4.10E-6 ID:SGTC_41|Compound:0478-0632|FD-Score:2.97|P-value:9.10E-4 ID:SGTC_57|Compound:miconazole|FD-Score:3.46|P-value:1.41E-4 ID:SGTC_252|Compound:miconazole|FD-Score:3.51|P-value:1.15E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:5.17|P-value:2.73E-8 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:-3.99|P-value:1.36E-5 ID:SGTC_393|Compound:acivicin|FD-Score:-3.51|P-value:1.09E-4 ID:SGTC_394|Compound:menadione|FD-Score:4.07|P-value:9.69E-6 ID:SGTC_620|Compound:0109-0052|FD-Score:-2.98|P-value:8.42E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:3.14|P-value:4.83E-4 ID:SGTC_622|Compound:1348-1566|FD-Score:5.27|P-value:1.55E-8 ID:SGTC_637|Compound:1181-0519|FD-Score:8.17|P-value:4.68E-18 ID:SGTC_646|Compound:4092-0839|FD-Score:3.61|P-value:7.63E-5 ID:SGTC_834|Compound:0312-0008|FD-Score:3.19|P-value:3.99E-4 ID:SGTC_838|Compound:0109-0203|FD-Score:3.24|P-value:3.39E-4 ID:SGTC_918|Compound:glabranin|FD-Score:3.22|P-value:3.62E-4 ID:SGTC_961|Compound:1252-1571|FD-Score:3.91|P-value:2.02E-5 ID:SGTC_1126|Compound:isoteolin|FD-Score:2.97|P-value:9.19E-4 ID:SGTC_1170|Compound:0310-0127|FD-Score:3.00|P-value:8.16E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:-3.95|P-value:1.64E-5 ID:SGTC_1252|Compound:0493-0875|FD-Score:-2.96|P-value:9.15E-4 ID:SGTC_1260|Compound:0700-1617|FD-Score:-3.12|P-value:5.10E-4 ID:SGTC_1331|Compound:1472-0049|FD-Score:-2.98|P-value:8.49E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:3.46|P-value:1.37E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:3.46|P-value:1.41E-4 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:3.45|P-value:1.47E-4 ID:SGTC_1754|Compound:st042065|FD-Score:3.13|P-value:4.99E-4 ID:SGTC_1850|Compound:st056238|FD-Score:4.93|P-value:1.12E-7 ID:SGTC_1864|Compound:st057645|FD-Score:3.27|P-value:3.04E-4 ID:SGTC_2024|Compound:5144227|FD-Score:3.57|P-value:9.06E-5 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.34|P-value:2.27E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:-4.20|P-value:4.95E-6 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-7.57|P-value:8.48E-16 ID:SGTC_2413|Compound:inermin|FD-Score:3.11|P-value:5.56E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-4.21|P-value:4.68E-6 ID:SGTC_2445|Compound:5742597|FD-Score:5.86|P-value:3.60E-10 ID:SGTC_2452|Compound:5863136|FD-Score:3.00|P-value:8.12E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:3.51|P-value:1.14E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:3.07|P-value:6.23E-4 ID:SGTC_2549|Compound:cedrol|FD-Score:-3.12|P-value:5.02E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:4.40|P-value:1.94E-6 ID:SGTC_2594|Compound:a-mangostin|FD-Score:-3.79|P-value:3.32E-5 ID:SGTC_2596|Compound:sinensetin|FD-Score:3.10|P-value:5.75E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:-3.78|P-value:3.53E-5 ID:SGTC_2791|Compound:5663188|FD-Score:3.26|P-value:3.13E-4 ID:SGTC_2800|Compound:7887935|FD-Score:-3.20|P-value:3.73E-4 ID:SGTC_2835|Compound:9006588|FD-Score:6.57|P-value:2.62E-12 ID:SGTC_2850|Compound:9015429|FD-Score:3.37|P-value:2.02E-4 ID:SGTC_2873|Compound:9040596|FD-Score:3.77|P-value:3.83E-5 ID:SGTC_2884|Compound:9058502|FD-Score:3.12|P-value:5.27E-4 ID:SGTC_2951|Compound:9072950|FD-Score:3.75|P-value:4.17E-5 ID:SGTC_3118|Compound:9124250|FD-Score:2.98|P-value:8.91E-4 ID:SGTC_3150|Compound:9097574|FD-Score:-3.74|P-value:4.13E-5 ID:SGTC_3151|Compound:9099821|FD-Score:-3.77|P-value:3.62E-5 ID:SGTC_3152|Compound:9099253|FD-Score:4.19|P-value:5.37E-6 ID:SGTC_3308|Compound:9128332|FD-Score:4.25|P-value:4.10E-6

Top fitness defect scores for YNL272C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_637 1181-0519 8.17 7.3 µM 4.68E-18 fatty acid desaturase (OLE1) 4 1.58E-16 1.28
2 SGTC_2835 9006588 6.57 71.4 µM 2.62E-12 2 2.58E-11 1.57
3 SGTC_2445 5742597 5.86 35.6 µM 3.60E-10 2 2.25E-9 1.53
4 SGTC_622 1348-1566 5.27 26.3 µM 1.55E-8 2 6.81E-8 1.34
5 SGTC_312 4092-0653 5.17 6.8 µM 2.73E-8 ergosterol depletion effects on membrane 19 1.14E-7 0.21
6 SGTC_1850 st056238 4.93 17.0 µM 1.12E-7 RPP1 & pyrimidine depletion 1 4.12E-7 0.77
7 SGTC_2566 crystal violet carbinol 4.40 226.0 nM 1.94E-6 12 5.49E-6 0.02
8 SGTC_3308 9128332 4.25 17.6 µM 4.10E-6 plasma membrane duress 8 1.09E-5 0.29
9 SGTC_3152 9099253 4.19 49.5 µM 5.37E-6 NEO1-PIK1 26 1.39E-5 0.11
10 SGTC_394 menadione 4.07 8.3 µM 9.69E-6 superoxide 4 2.37E-5 0.03
11 SGTC_961 1252-1571 3.91 372.0 µM 2.02E-5 3 4.64E-5 0.09
12 SGTC_2873 9040596 3.77 71.4 µM 3.83E-5 6 8.29E-5 0.05
13 SGTC_2951 9072950 3.75 58.1 µM 4.17E-5 ERG2 7 8.96E-5 0.01
14 SGTC_646 4092-0839 3.61 27.3 µM 7.63E-5 plasma membrane duress 12 1.55E-4 0.02
15 SGTC_2024 5144227 3.57 134.0 µM 9.06E-5 7 1.82E-4 0.01
16 SGTC_2540 epiandrosterone 3.51 100.0 µM 1.14E-4 7 2.23E-4 0.10
17 SGTC_252 miconazole 3.51 1.3 nM 1.15E-4 ergosterol depletion effects on membrane 20 2.26E-4 0.05
18 SGTC_1368 1852-0141 3.46 284.0 µM 1.37E-4 ERG2 6 2.65E-4 0.02
19 SGTC_1562 morin hydrate 3.46 59.1 µM 1.41E-4 11 2.71E-4 0.10
20 SGTC_57 miconazole 3.46 162.5 nM 1.41E-4 ergosterol depletion effects on membrane 30 2.71E-4 0.02

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.170 4.81E-23 YGL233W SEC15 het Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase
0.166 2.96E-22 YBR102C EXO84 het Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
0.154 2.20E-19 YLR350W ORM2 hom Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress
0.150 2.98E-18 YPR055W SEC8 het Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress
0.145 4.13E-17 YJL207C LAA1 hom AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene
0.138 9.90E-16 YFL034W_p YFL034W_p hom Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk
0.131 2.89E-14 YMR218C TRS130 het One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy
0.130 4.66E-14 YML037C_p YML037C_p hom Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
0.128 1.16E-13 YIL068C SEC6 het Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p
0.127 1.56E-13 YMR071C TVP18 hom Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments
0.124 5.55E-13 YLR057W MNL2 hom Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene
0.124 6.34E-13 YJL144W YJL144W hom Cytoplasmic hydrophilin essential in dessication-rehydration process; expression induced by osmotic stress, starvation and during stationary phase; protein abundance increases in response to DNA replication stress
0.124 6.71E-13 YGR060W ERG25 het C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol
0.119 5.38E-12 YLR170C APS1 hom Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex
0.118 6.39E-12 YPL259C APM1 hom Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved in clathrin-dependent Golgi protein sorting