YNL287W / SEC21

Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo

Zygosity: Heterozygous strain
fixedexpanded
Profile for YNL287W / SEC21

Click on Significant Values for Screen Details ID:SGTC_3|Compound:0833-0587|FD-Score:3.69|P-value:3.64E-4 ID:SGTC_22|Compound:0964-0034|FD-Score:7.10|P-value:3.88E-11 ID:SGTC_56|Compound:0957-0365|FD-Score:3.79|P-value:2.63E-4 ID:SGTC_81|Compound:0411-0046|FD-Score:-4.19|P-value:5.95E-5 ID:SGTC_91|Compound:0417-1751|FD-Score:5.08|P-value:1.65E-6 ID:SGTC_103|Compound:0844-0104|FD-Score:9.66|P-value:4.36E-19 ID:SGTC_110|Compound:0850-0284|FD-Score:3.70|P-value:3.49E-4 ID:SGTC_123|Compound:0083-0120|FD-Score:5.58|P-value:1.59E-7 ID:SGTC_245|Compound:methotrexate|FD-Score:5.84|P-value:4.49E-8 ID:SGTC_255|Compound:tunicamycin|FD-Score:21.80|P-value:2.50E-89 ID:SGTC_256|Compound:clotrimazole|FD-Score:-4.02|P-value:1.13E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.91|P-value:1.69E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:-4.96|P-value:2.70E-6 ID:SGTC_373|Compound:0844-0104|FD-Score:7.88|P-value:2.54E-13 ID:SGTC_384|Compound:k007-0144|FD-Score:4.44|P-value:2.41E-5 ID:SGTC_394|Compound:menadione|FD-Score:3.38|P-value:9.78E-4 ID:SGTC_400|Compound:oxethazaine|FD-Score:4.03|P-value:1.10E-4 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:3.41|P-value:8.95E-4 ID:SGTC_426|Compound:0988-0037|FD-Score:4.93|P-value:3.15E-6 ID:SGTC_459|Compound:nigericin|FD-Score:4.14|P-value:7.62E-5 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:-4.15|P-value:7.13E-5 ID:SGTC_514|Compound:4466-0038|FD-Score:-3.71|P-value:3.31E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:3.81|P-value:2.45E-4 ID:SGTC_637|Compound:1181-0519|FD-Score:-4.67|P-value:9.15E-6 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.72|P-value:3.33E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:21.40|P-value:3.58E-86 ID:SGTC_805|Compound:3852-0218|FD-Score:4.33|P-value:3.72E-5 ID:SGTC_918|Compound:glabranin|FD-Score:4.00|P-value:1.26E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:3.57|P-value:5.42E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:5.31|P-value:5.86E-7 ID:SGTC_1026|Compound:k007-1105|FD-Score:6.18|P-value:7.58E-9 ID:SGTC_1051|Compound:3253-3903|FD-Score:-4.22|P-value:5.36E-5 ID:SGTC_1055|Compound:3448-0672|FD-Score:-4.68|P-value:8.91E-6 ID:SGTC_1060|Compound:3448-7350|FD-Score:7.79|P-value:4.84E-13 ID:SGTC_1098|Compound:clofazimine|FD-Score:-3.96|P-value:1.38E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-3.78|P-value:2.58E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:-3.63|P-value:4.30E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:14.30|P-value:1.77E-39 ID:SGTC_1165|Compound:k072-0275|FD-Score:4.53|P-value:1.67E-5 ID:SGTC_1259|Compound:0671-0132|FD-Score:-4.35|P-value:3.35E-5 ID:SGTC_1260|Compound:0700-1617|FD-Score:3.64|P-value:4.35E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:5.17|P-value:1.10E-6 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.79|P-value:2.63E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:5.96|P-value:2.44E-8 ID:SGTC_1724|Compound:st037281|FD-Score:3.64|P-value:4.30E-4 ID:SGTC_1754|Compound:st042065|FD-Score:-5.45|P-value:2.91E-7 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:4.64|P-value:1.08E-5 ID:SGTC_1789|Compound:tunicamycin|FD-Score:15.50|P-value:5.11E-46 ID:SGTC_1800|Compound:st050079|FD-Score:-5.50|P-value:2.23E-7 ID:SGTC_1818|Compound:st052136|FD-Score:-5.46|P-value:2.74E-7 ID:SGTC_1885|Compound:st057546|FD-Score:4.02|P-value:1.15E-4 ID:SGTC_1895|Compound:st058434|FD-Score:3.68|P-value:3.77E-4 ID:SGTC_1982|Compound:st072467|FD-Score:3.48|P-value:7.31E-4 ID:SGTC_2001|Compound:4023502|FD-Score:-4.02|P-value:1.13E-4 ID:SGTC_2007|Compound:4011539|FD-Score:5.80|P-value:5.40E-8 ID:SGTC_2009|Compound:4012297|FD-Score:8.91|P-value:1.59E-16 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:7.23|P-value:1.74E-11 ID:SGTC_2284|Compound:7945075|FD-Score:-3.47|P-value:7.27E-4 ID:SGTC_2291|Compound:7917025|FD-Score:3.86|P-value:2.01E-4 ID:SGTC_2313|Compound:7726885|FD-Score:6.72|P-value:3.79E-10 ID:SGTC_2379|Compound:9035213|FD-Score:4.71|P-value:8.13E-6 ID:SGTC_2392|Compound:N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide|FD-Score:3.53|P-value:6.25E-4 ID:SGTC_2413|Compound:inermin|FD-Score:9.64|P-value:5.03E-19 ID:SGTC_2432|Compound:5630864|FD-Score:3.48|P-value:7.13E-4 ID:SGTC_2556|Compound:citropten|FD-Score:3.60|P-value:4.83E-4 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:3.81|P-value:2.47E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:-3.94|P-value:1.51E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:-3.37|P-value:9.86E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:6.67|P-value:5.02E-10 ID:SGTC_2955|Compound:9076254|FD-Score:4.34|P-value:3.50E-5 ID:SGTC_2959|Compound:9079155|FD-Score:8.16|P-value:3.89E-14 ID:SGTC_2960|Compound:9079389|FD-Score:4.09|P-value:9.05E-5 ID:SGTC_2993|Compound:9054257|FD-Score:5.71|P-value:8.47E-8 ID:SGTC_3103|Compound:9120822|FD-Score:4.62|P-value:1.16E-5 ID:SGTC_3105|Compound:9121040|FD-Score:4.12|P-value:8.06E-5 ID:SGTC_3142|Compound:9086498|FD-Score:7.66|P-value:1.17E-12 ID:SGTC_3295|Compound:9122362|FD-Score:-3.79|P-value:2.50E-4 ID:SGTC_3|Compound:0833-0587|FD-Score:3.69|P-value:3.64E-4 ID:SGTC_22|Compound:0964-0034|FD-Score:7.10|P-value:3.88E-11 ID:SGTC_56|Compound:0957-0365|FD-Score:3.79|P-value:2.63E-4 ID:SGTC_81|Compound:0411-0046|FD-Score:-4.19|P-value:5.95E-5 ID:SGTC_91|Compound:0417-1751|FD-Score:5.08|P-value:1.65E-6 ID:SGTC_103|Compound:0844-0104|FD-Score:9.66|P-value:4.36E-19 ID:SGTC_110|Compound:0850-0284|FD-Score:3.70|P-value:3.49E-4 ID:SGTC_123|Compound:0083-0120|FD-Score:5.58|P-value:1.59E-7 ID:SGTC_245|Compound:methotrexate|FD-Score:5.84|P-value:4.49E-8 ID:SGTC_255|Compound:tunicamycin|FD-Score:21.80|P-value:2.50E-89 ID:SGTC_256|Compound:clotrimazole|FD-Score:-4.02|P-value:1.13E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.91|P-value:1.69E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:-4.96|P-value:2.70E-6 ID:SGTC_373|Compound:0844-0104|FD-Score:7.88|P-value:2.54E-13 ID:SGTC_384|Compound:k007-0144|FD-Score:4.44|P-value:2.41E-5 ID:SGTC_394|Compound:menadione|FD-Score:3.38|P-value:9.78E-4 ID:SGTC_400|Compound:oxethazaine|FD-Score:4.03|P-value:1.10E-4 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:3.41|P-value:8.95E-4 ID:SGTC_426|Compound:0988-0037|FD-Score:4.93|P-value:3.15E-6 ID:SGTC_459|Compound:nigericin|FD-Score:4.14|P-value:7.62E-5 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:-4.15|P-value:7.13E-5 ID:SGTC_514|Compound:4466-0038|FD-Score:-3.71|P-value:3.31E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:3.81|P-value:2.45E-4 ID:SGTC_637|Compound:1181-0519|FD-Score:-4.67|P-value:9.15E-6 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.72|P-value:3.33E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:21.40|P-value:3.58E-86 ID:SGTC_805|Compound:3852-0218|FD-Score:4.33|P-value:3.72E-5 ID:SGTC_918|Compound:glabranin|FD-Score:4.00|P-value:1.26E-4 ID:SGTC_943|Compound:3253-1379|FD-Score:3.57|P-value:5.42E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:5.31|P-value:5.86E-7 ID:SGTC_1026|Compound:k007-1105|FD-Score:6.18|P-value:7.58E-9 ID:SGTC_1051|Compound:3253-3903|FD-Score:-4.22|P-value:5.36E-5 ID:SGTC_1055|Compound:3448-0672|FD-Score:-4.68|P-value:8.91E-6 ID:SGTC_1060|Compound:3448-7350|FD-Score:7.79|P-value:4.84E-13 ID:SGTC_1098|Compound:clofazimine|FD-Score:-3.96|P-value:1.38E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-3.78|P-value:2.58E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:-3.63|P-value:4.30E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:14.30|P-value:1.77E-39 ID:SGTC_1165|Compound:k072-0275|FD-Score:4.53|P-value:1.67E-5 ID:SGTC_1259|Compound:0671-0132|FD-Score:-4.35|P-value:3.35E-5 ID:SGTC_1260|Compound:0700-1617|FD-Score:3.64|P-value:4.35E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:5.17|P-value:1.10E-6 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.79|P-value:2.63E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:5.96|P-value:2.44E-8 ID:SGTC_1724|Compound:st037281|FD-Score:3.64|P-value:4.30E-4 ID:SGTC_1754|Compound:st042065|FD-Score:-5.45|P-value:2.91E-7 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:4.64|P-value:1.08E-5 ID:SGTC_1789|Compound:tunicamycin|FD-Score:15.50|P-value:5.11E-46 ID:SGTC_1800|Compound:st050079|FD-Score:-5.50|P-value:2.23E-7 ID:SGTC_1818|Compound:st052136|FD-Score:-5.46|P-value:2.74E-7 ID:SGTC_1885|Compound:st057546|FD-Score:4.02|P-value:1.15E-4 ID:SGTC_1895|Compound:st058434|FD-Score:3.68|P-value:3.77E-4 ID:SGTC_1982|Compound:st072467|FD-Score:3.48|P-value:7.31E-4 ID:SGTC_2001|Compound:4023502|FD-Score:-4.02|P-value:1.13E-4 ID:SGTC_2007|Compound:4011539|FD-Score:5.80|P-value:5.40E-8 ID:SGTC_2009|Compound:4012297|FD-Score:8.91|P-value:1.59E-16 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:7.23|P-value:1.74E-11 ID:SGTC_2284|Compound:7945075|FD-Score:-3.47|P-value:7.27E-4 ID:SGTC_2291|Compound:7917025|FD-Score:3.86|P-value:2.01E-4 ID:SGTC_2313|Compound:7726885|FD-Score:6.72|P-value:3.79E-10 ID:SGTC_2379|Compound:9035213|FD-Score:4.71|P-value:8.13E-6 ID:SGTC_2392|Compound:N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide|FD-Score:3.53|P-value:6.25E-4 ID:SGTC_2413|Compound:inermin|FD-Score:9.64|P-value:5.03E-19 ID:SGTC_2432|Compound:5630864|FD-Score:3.48|P-value:7.13E-4 ID:SGTC_2556|Compound:citropten|FD-Score:3.60|P-value:4.83E-4 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:3.81|P-value:2.47E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:-3.94|P-value:1.51E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:-3.37|P-value:9.86E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:6.67|P-value:5.02E-10 ID:SGTC_2955|Compound:9076254|FD-Score:4.34|P-value:3.50E-5 ID:SGTC_2959|Compound:9079155|FD-Score:8.16|P-value:3.89E-14 ID:SGTC_2960|Compound:9079389|FD-Score:4.09|P-value:9.05E-5 ID:SGTC_2993|Compound:9054257|FD-Score:5.71|P-value:8.47E-8 ID:SGTC_3103|Compound:9120822|FD-Score:4.62|P-value:1.16E-5 ID:SGTC_3105|Compound:9121040|FD-Score:4.12|P-value:8.06E-5 ID:SGTC_3142|Compound:9086498|FD-Score:7.66|P-value:1.17E-12 ID:SGTC_3295|Compound:9122362|FD-Score:-3.79|P-value:2.50E-4

Top fitness defect scores for YNL287W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_255 tunicamycin 21.80 200.5 nM 2.50E-89 unfolded protein response 2 6.05E-106 3.21
2 SGTC_733 4069-0007 21.40 56.1 µM 3.58E-86 ERAD & cell cycle 1 3.45E-102 6.42
3 SGTC_1789 tunicamycin 15.50 25.0 nM 5.11E-46 unfolded protein response 2 2.08E-54 1.71
4 SGTC_1161 k007-0610 14.30 110.0 µM 1.77E-39 ERAD & cell cycle 4 1.25E-46 5.18
5 SGTC_103 0844-0104 9.66 51.8 µM 4.36E-19 ERAD & cell cycle 10 2.22E-22 0.86
6 SGTC_2413 inermin 9.64 26.1 µM 5.03E-19 6 2.64E-22 2.08
7 SGTC_2009 4012297 8.91 12.1 µM 1.59E-16 cell wall signaling 2 2.47E-19 2.47
8 SGTC_2959 9079155 8.16 11.0 µM 3.89E-14 1 1.69E-16 1.73
9 SGTC_373 0844-0104 7.88 25.9 µM 2.54E-13 ERAD & cell cycle 1 1.58E-15 1.04
10 SGTC_1060 3448-7350 7.79 82.0 µM 4.84E-13 Golgi 4 3.38E-15 0.14
11 SGTC_3142 9086498 7.66 49.5 µM 1.17E-12 iron homeostasis 15 9.61E-15 0.09
12 SGTC_2232 chlorophetanol 7.23 41.5 µM 1.74E-11 TRP & mitochondrial translation 3 2.39E-13 1.42
13 SGTC_22 0964-0034 7.10 14.5 µM 3.88E-11 unfolded protein response 8 6.16E-13 0.12
14 SGTC_2313 7726885 6.72 200.0 µM 3.79E-10 RPP1 & pyrimidine depletion 1 9.21E-12 1.20
15 SGTC_2779 methotrexate 6.67 400.0 µM 5.02E-10 3 1.29E-11 0.76
16 SGTC_1026 k007-1105 6.18 30.6 µM 7.58E-9 ERAD & cell cycle 16 3.22E-10 0.83
17 SGTC_1597 7-benzyloxygramine 5.96 47.5 µM 2.44E-8 calcium & mitochondrial duress 36 1.29E-9 0.28
18 SGTC_245 methotrexate 5.84 289.6 µM 4.49E-8 7 2.65E-9 0.24
19 SGTC_2007 4011539 5.80 71.8 µM 5.40E-8 cell wall signaling 2 3.31E-9 0.78
20 SGTC_2993 9054257 5.71 71.4 µM 8.47E-8 fatty acid desaturase (OLE1) 17 5.63E-9 0.20

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.436 3.80E-156 YDR238C SEC26 het Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
0.430 3.84E-151 YBR243C ALG7 het UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin
0.388 2.67E-121 YIL030C SSM4 hom Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation
0.383 2.34E-117 YFR051C RET2 het Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
0.351 4.04E-98 YDL145C COP1 het Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway
0.340 1.13E-91 YOR246C ENV9 hom Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
0.327 2.39E-84 YDR186C YDR186C hom Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.316 1.24E-78 YOL002C IZH2 hom Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc
0.282 2.52E-62 YER084W_p YER084W_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.267 5.89E-56 YGR196C FYV8 hom Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.252 9.49E-50 YMR029C FAR8 hom Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p
0.245 4.80E-47 YMR022W UBC7 hom Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
0.237 5.74E-44 YDR199W_d YDR199W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity
0.233 1.75E-42 YDL100C GET3 hom Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress
0.225 6.39E-40 YER083C GET2 hom Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division