YNL293W / MSB3

Rab GTPase-activating protein; regulates endocytosis via activation of Vps21p and Ypt7p; also acts on Ypt52p and Sec4p; also required for proper actin organization; localizes to the plasma membrane and sites of polarized growth, and a portion localizes to endosomes and vacuoles; similar to Msb4p; relocalizes from bud neck to cytoplasm upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL293W / MSB3

Click on Significant Values for Screen Details ID:SGTC_22|Compound:0964-0034|FD-Score:-3.25|P-value:7.78E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:3.20|P-value:8.03E-4 ID:SGTC_253|Compound:dyclonine|FD-Score:-3.19|P-value:9.44E-4 ID:SGTC_292|Compound:k038-0015|FD-Score:3.29|P-value:5.90E-4 ID:SGTC_308|Compound:4092-1169|FD-Score:4.16|P-value:2.08E-5 ID:SGTC_312|Compound:4092-0653|FD-Score:3.40|P-value:4.09E-4 ID:SGTC_373|Compound:0844-0104|FD-Score:3.80|P-value:9.14E-5 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:3.23|P-value:7.31E-4 ID:SGTC_435|Compound:c-PAF|FD-Score:-3.21|P-value:8.83E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-7.22|P-value:8.63E-13 ID:SGTC_455|Compound:paf C-16|FD-Score:-3.20|P-value:9.01E-4 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:3.36|P-value:4.72E-4 ID:SGTC_493|Compound:zm226600|FD-Score:3.56|P-value:2.29E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:3.80|P-value:9.30E-5 ID:SGTC_808|Compound:0711-0032|FD-Score:6.31|P-value:2.75E-10 ID:SGTC_834|Compound:0312-0008|FD-Score:3.66|P-value:1.55E-4 ID:SGTC_881|Compound:0833-0569|FD-Score:4.56|P-value:3.60E-6 ID:SGTC_910|Compound:3454-3003|FD-Score:-5.93|P-value:3.54E-9 ID:SGTC_912|Compound:3474-0017|FD-Score:3.38|P-value:4.28E-4 ID:SGTC_1019|Compound:4262-0212|FD-Score:3.43|P-value:3.68E-4 ID:SGTC_1123|Compound:mangostin|FD-Score:4.04|P-value:3.46E-5 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.94|P-value:5.27E-5 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:-4.36|P-value:1.03E-5 ID:SGTC_1187|Compound:1391-0724|FD-Score:3.45|P-value:3.44E-4 ID:SGTC_1378|Compound:2093-0007|FD-Score:4.76|P-value:1.42E-6 ID:SGTC_1446|Compound:4239-0190|FD-Score:3.61|P-value:1.91E-4 ID:SGTC_1500|Compound:4488-1866|FD-Score:4.65|P-value:2.40E-6 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.58|P-value:2.09E-4 ID:SGTC_1709|Compound:st033179|FD-Score:3.32|P-value:5.41E-4 ID:SGTC_1712|Compound:st032288|FD-Score:4.81|P-value:1.12E-6 ID:SGTC_1739|Compound:st037196|FD-Score:4.70|P-value:1.90E-6 ID:SGTC_1740|Compound:st038286|FD-Score:3.19|P-value:8.32E-4 ID:SGTC_1945|Compound:st075991|FD-Score:-3.58|P-value:2.37E-4 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:3.22|P-value:7.43E-4 ID:SGTC_2006|Compound:4011487|FD-Score:10.40|P-value:5.87E-25 ID:SGTC_2021|Compound:4019055|FD-Score:3.32|P-value:5.35E-4 ID:SGTC_2142|Compound:5622915|FD-Score:-3.62|P-value:2.09E-4 ID:SGTC_2332|Compound:7982454|FD-Score:-6.09|P-value:1.31E-9 ID:SGTC_2337|Compound:9009472|FD-Score:3.96|P-value:4.86E-5 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:4.05|P-value:3.35E-5 ID:SGTC_2576|Compound:guaiol|FD-Score:-3.27|P-value:7.20E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:6.24|P-value:4.36E-10 ID:SGTC_2591|Compound:skatole|FD-Score:3.27|P-value:6.36E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.69|P-value:1.13E-8 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:-3.66|P-value:1.80E-4 ID:SGTC_2864|Compound:9034755|FD-Score:3.18|P-value:8.66E-4 ID:SGTC_2896|Compound:9052656|FD-Score:3.63|P-value:1.77E-4 ID:SGTC_3037|Compound:9091026|FD-Score:3.45|P-value:3.41E-4 ID:SGTC_3079|Compound:9117512|FD-Score:3.35|P-value:4.87E-4 ID:SGTC_3085|Compound:9117376|FD-Score:4.84|P-value:9.58E-7 ID:SGTC_3093|Compound:9117344|FD-Score:-3.20|P-value:9.20E-4 ID:SGTC_3325|Compound:9138760|FD-Score:3.65|P-value:1.64E-4 ID:SGTC_3355|Compound:9151058|FD-Score:3.42|P-value:3.71E-4 ID:SGTC_22|Compound:0964-0034|FD-Score:-3.25|P-value:7.78E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:3.20|P-value:8.03E-4 ID:SGTC_253|Compound:dyclonine|FD-Score:-3.19|P-value:9.44E-4 ID:SGTC_292|Compound:k038-0015|FD-Score:3.29|P-value:5.90E-4 ID:SGTC_308|Compound:4092-1169|FD-Score:4.16|P-value:2.08E-5 ID:SGTC_312|Compound:4092-0653|FD-Score:3.40|P-value:4.09E-4 ID:SGTC_373|Compound:0844-0104|FD-Score:3.80|P-value:9.14E-5 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:3.23|P-value:7.31E-4 ID:SGTC_435|Compound:c-PAF|FD-Score:-3.21|P-value:8.83E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-7.22|P-value:8.63E-13 ID:SGTC_455|Compound:paf C-16|FD-Score:-3.20|P-value:9.01E-4 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:3.36|P-value:4.72E-4 ID:SGTC_493|Compound:zm226600|FD-Score:3.56|P-value:2.29E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:3.80|P-value:9.30E-5 ID:SGTC_808|Compound:0711-0032|FD-Score:6.31|P-value:2.75E-10 ID:SGTC_834|Compound:0312-0008|FD-Score:3.66|P-value:1.55E-4 ID:SGTC_881|Compound:0833-0569|FD-Score:4.56|P-value:3.60E-6 ID:SGTC_910|Compound:3454-3003|FD-Score:-5.93|P-value:3.54E-9 ID:SGTC_912|Compound:3474-0017|FD-Score:3.38|P-value:4.28E-4 ID:SGTC_1019|Compound:4262-0212|FD-Score:3.43|P-value:3.68E-4 ID:SGTC_1123|Compound:mangostin|FD-Score:4.04|P-value:3.46E-5 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.94|P-value:5.27E-5 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:-4.36|P-value:1.03E-5 ID:SGTC_1187|Compound:1391-0724|FD-Score:3.45|P-value:3.44E-4 ID:SGTC_1378|Compound:2093-0007|FD-Score:4.76|P-value:1.42E-6 ID:SGTC_1446|Compound:4239-0190|FD-Score:3.61|P-value:1.91E-4 ID:SGTC_1500|Compound:4488-1866|FD-Score:4.65|P-value:2.40E-6 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.58|P-value:2.09E-4 ID:SGTC_1709|Compound:st033179|FD-Score:3.32|P-value:5.41E-4 ID:SGTC_1712|Compound:st032288|FD-Score:4.81|P-value:1.12E-6 ID:SGTC_1739|Compound:st037196|FD-Score:4.70|P-value:1.90E-6 ID:SGTC_1740|Compound:st038286|FD-Score:3.19|P-value:8.32E-4 ID:SGTC_1945|Compound:st075991|FD-Score:-3.58|P-value:2.37E-4 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:3.22|P-value:7.43E-4 ID:SGTC_2006|Compound:4011487|FD-Score:10.40|P-value:5.87E-25 ID:SGTC_2021|Compound:4019055|FD-Score:3.32|P-value:5.35E-4 ID:SGTC_2142|Compound:5622915|FD-Score:-3.62|P-value:2.09E-4 ID:SGTC_2332|Compound:7982454|FD-Score:-6.09|P-value:1.31E-9 ID:SGTC_2337|Compound:9009472|FD-Score:3.96|P-value:4.86E-5 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:4.05|P-value:3.35E-5 ID:SGTC_2576|Compound:guaiol|FD-Score:-3.27|P-value:7.20E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:6.24|P-value:4.36E-10 ID:SGTC_2591|Compound:skatole|FD-Score:3.27|P-value:6.36E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.69|P-value:1.13E-8 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:-3.66|P-value:1.80E-4 ID:SGTC_2864|Compound:9034755|FD-Score:3.18|P-value:8.66E-4 ID:SGTC_2896|Compound:9052656|FD-Score:3.63|P-value:1.77E-4 ID:SGTC_3037|Compound:9091026|FD-Score:3.45|P-value:3.41E-4 ID:SGTC_3079|Compound:9117512|FD-Score:3.35|P-value:4.87E-4 ID:SGTC_3085|Compound:9117376|FD-Score:4.84|P-value:9.58E-7 ID:SGTC_3093|Compound:9117344|FD-Score:-3.20|P-value:9.20E-4 ID:SGTC_3325|Compound:9138760|FD-Score:3.65|P-value:1.64E-4 ID:SGTC_3355|Compound:9151058|FD-Score:3.42|P-value:3.71E-4

Top fitness defect scores for YNL293W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2006 4011487 10.40 200.0 µM 5.87E-25 endomembrane recycling 17 7.97E-26
2 SGTC_808 0711-0032 6.31 135.0 µM 2.75E-10 3 1.37E-10
3 SGTC_2582 agaric acid 6.24 100.0 µM 4.36E-10 redox potentiating 148 2.21E-10
4 SGTC_2688 kanamycin b 5.69 96.7 µM 1.13E-8 calcium & mitochondrial duress 183 6.45E-9
5 SGTC_3085 9117376 4.84 49.5 µM 9.58E-7 3 6.46E-7
6 SGTC_1712 st032288 4.81 53.4 µM 1.12E-6 calcium & mitochondrial duress 129 7.58E-7
7 SGTC_1378 2093-0007 4.76 380.0 nM 1.42E-6 7 9.70E-7
8 SGTC_1739 st037196 4.70 92.5 µM 1.90E-6 8 1.31E-6
9 SGTC_1500 4488-1866 4.65 171.0 µM 2.40E-6 RPP1 & pyrimidine depletion 6 1.67E-6
10 SGTC_881 0833-0569 4.56 170.0 µM 3.60E-6 11 2.54E-6
11 SGTC_308 4092-1169 4.16 104.0 µM 2.08E-5 plasma membrane duress 46 1.57E-5
12 SGTC_2506 ethyl hematommate 4.05 54.2 µM 3.35E-5 RPP1 & pyrimidine depletion 15 2.56E-5
13 SGTC_1123 mangostin 4.04 811.3 nM 3.46E-5 mitochondrial response to ROS 35 2.65E-5
14 SGTC_2337 9009472 3.96 200.0 µM 4.86E-5 32 3.76E-5
15 SGTC_1125 tnp00248 3.94 1.3 µM 5.27E-5 26 4.09E-5
16 SGTC_373 0844-0104 3.80 25.9 µM 9.14E-5 ERAD & cell cycle 53 7.24E-5
17 SGTC_528 3492-0019 3.80 172.0 µM 9.30E-5 calcium & mitochondrial duress 234 7.37E-5
18 SGTC_834 0312-0008 3.66 75.7 µM 1.55E-4 NEO1 50 1.25E-4
19 SGTC_3325 9138760 3.65 55.2 µM 1.64E-4 Golgi 35 1.33E-4
20 SGTC_2896 9052656 3.63 71.4 µM 1.77E-4 endomembrane recycling 140 1.43E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.234 6.64E-43 YFL034W_p YFL034W_p hom Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk
0.222 7.75E-39 YDR084C TVP23 hom Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
0.218 1.84E-37 YKL051W SFK1 hom Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane
0.203 1.21E-32 YFL033C RIM15 hom Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase
0.202 3.87E-32 YLR170C APS1 hom Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex
0.201 8.55E-32 YLR171W_d YLR171W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.195 3.56E-30 YKL135C APL2 hom Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress
0.193 1.56E-29 YJL094C KHA1 hom Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies
0.192 4.60E-29 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.189 2.13E-28 YHL019C APM2 hom Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport
0.185 3.98E-27 YDL019C OSH2 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.183 1.02E-26 YBL022C PIM1 hom ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria
0.180 7.79E-26 YJR040W GEF1 hom Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism
0.179 1.37E-25 YJL165C HAL5 hom Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication
0.174 2.65E-24 YMR099C YMR099C hom Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS