YNL296W_d

Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL296W / YNL296W

Click on Significant Values for Screen Details ID:SGTC_43|Compound:0570-0105|FD-Score:3.87|P-value:1.03E-4 ID:SGTC_50|Compound:0929-0078|FD-Score:3.97|P-value:6.95E-5 ID:SGTC_100|Compound:0086-0128|FD-Score:-3.79|P-value:6.28E-5 ID:SGTC_113|Compound:0099-0221|FD-Score:-3.23|P-value:5.06E-4 ID:SGTC_139|Compound:0099-0334|FD-Score:3.68|P-value:2.15E-4 ID:SGTC_156|Compound:3474-0145|FD-Score:-4.25|P-value:8.78E-6 ID:SGTC_229|Compound:benomyl|FD-Score:3.55|P-value:3.45E-4 ID:SGTC_411|Compound:enrofloxacin|FD-Score:3.41|P-value:5.61E-4 ID:SGTC_458|Compound:nppb|FD-Score:3.47|P-value:4.60E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:-3.66|P-value:1.04E-4 ID:SGTC_758|Compound:0868-0029|FD-Score:-4.23|P-value:9.68E-6 ID:SGTC_893|Compound:0958-0369|FD-Score:-3.37|P-value:3.11E-4 ID:SGTC_952|Compound:1185-0122|FD-Score:3.34|P-value:7.13E-4 ID:SGTC_1409|Compound:3909-8004|FD-Score:4.17|P-value:3.12E-5 ID:SGTC_1412|Compound:3909-8563|FD-Score:3.35|P-value:7.05E-4 ID:SGTC_1420|Compound:4'-methoxyflavone|FD-Score:4.09|P-value:4.42E-5 ID:SGTC_1429|Compound:4049-0205|FD-Score:3.74|P-value:1.73E-4 ID:SGTC_1480|Compound:1070-0044|FD-Score:3.83|P-value:1.21E-4 ID:SGTC_1513|Compound:0337-0559|FD-Score:3.39|P-value:6.16E-4 ID:SGTC_1571|Compound:st070080|FD-Score:3.79|P-value:1.44E-4 ID:SGTC_1573|Compound:oleylamine|FD-Score:-3.78|P-value:6.63E-5 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:4.98|P-value:7.88E-7 ID:SGTC_1856|Compound:st057054|FD-Score:-3.68|P-value:9.66E-5 ID:SGTC_1868|Compound:st057644|FD-Score:3.95|P-value:7.59E-5 ID:SGTC_1911|Compound:st060208|FD-Score:4.22|P-value:2.49E-5 ID:SGTC_1915|Compound:st067105|FD-Score:3.96|P-value:7.36E-5 ID:SGTC_1944|Compound:st076371|FD-Score:4.38|P-value:1.29E-5 ID:SGTC_1953|Compound:st076800|FD-Score:4.38|P-value:1.29E-5 ID:SGTC_1963|Compound:st076565|FD-Score:3.73|P-value:1.76E-4 ID:SGTC_1968|Compound:st069590|FD-Score:4.08|P-value:4.52E-5 ID:SGTC_1982|Compound:st072467|FD-Score:4.22|P-value:2.58E-5 ID:SGTC_2080|Compound:5212524|FD-Score:3.32|P-value:7.71E-4 ID:SGTC_2123|Compound:5259487|FD-Score:3.36|P-value:6.70E-4 ID:SGTC_2128|Compound:5255449|FD-Score:4.95|P-value:8.94E-7 ID:SGTC_2219|Compound:9098279|FD-Score:-3.19|P-value:5.77E-4 ID:SGTC_2303|Compound:7774051|FD-Score:-3.18|P-value:6.00E-4 ID:SGTC_2336|Compound:9001819|FD-Score:4.96|P-value:8.84E-7 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:-3.97|P-value:2.99E-5 ID:SGTC_2458|Compound:5808907|FD-Score:-3.36|P-value:3.18E-4 ID:SGTC_2460|Compound:5552655|FD-Score:3.85|P-value:1.11E-4 ID:SGTC_2464|Compound:5717415|FD-Score:-3.04|P-value:9.83E-4 ID:SGTC_2466|Compound:5983409|FD-Score:3.74|P-value:1.73E-4 ID:SGTC_2467|Compound:5474540|FD-Score:-5.60|P-value:9.62E-9 ID:SGTC_2470|Compound:5664817|FD-Score:3.54|P-value:3.63E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:4.72|P-value:2.77E-6 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:4.31|P-value:1.76E-5 ID:SGTC_2601|Compound:securinine|FD-Score:4.55|P-value:6.11E-6 ID:SGTC_2689|Compound:tacrolimus|FD-Score:4.51|P-value:7.28E-6 ID:SGTC_2830|Compound:7992007|FD-Score:3.61|P-value:2.73E-4 ID:SGTC_2834|Compound:9001755|FD-Score:4.24|P-value:2.35E-5 ID:SGTC_2875|Compound:9039966|FD-Score:3.36|P-value:6.63E-4 ID:SGTC_2882|Compound:9058044|FD-Score:3.36|P-value:6.79E-4 ID:SGTC_2913|Compound:7966556|FD-Score:-3.16|P-value:6.55E-4 ID:SGTC_2922|Compound:7999257|FD-Score:3.69|P-value:2.08E-4 ID:SGTC_2926|Compound:9010726|FD-Score:-3.34|P-value:3.42E-4 ID:SGTC_3052|Compound:9092717|FD-Score:3.29|P-value:8.64E-4 ID:SGTC_3093|Compound:9117344|FD-Score:4.35|P-value:1.46E-5 ID:SGTC_3097|Compound:9117246|FD-Score:3.71|P-value:1.91E-4 ID:SGTC_3131|Compound:9127911|FD-Score:5.02|P-value:6.33E-7 ID:SGTC_3188|Compound:9110789|FD-Score:3.25|P-value:9.84E-4 ID:SGTC_3218|Compound:9131285|FD-Score:3.26|P-value:9.58E-4 ID:SGTC_3331|Compound:9141060|FD-Score:3.94|P-value:7.77E-5 ID:SGTC_3334|Compound:9142812|FD-Score:3.31|P-value:8.03E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:3.87|P-value:1.03E-4 ID:SGTC_50|Compound:0929-0078|FD-Score:3.97|P-value:6.95E-5 ID:SGTC_100|Compound:0086-0128|FD-Score:-3.79|P-value:6.28E-5 ID:SGTC_113|Compound:0099-0221|FD-Score:-3.23|P-value:5.06E-4 ID:SGTC_139|Compound:0099-0334|FD-Score:3.68|P-value:2.15E-4 ID:SGTC_156|Compound:3474-0145|FD-Score:-4.25|P-value:8.78E-6 ID:SGTC_229|Compound:benomyl|FD-Score:3.55|P-value:3.45E-4 ID:SGTC_411|Compound:enrofloxacin|FD-Score:3.41|P-value:5.61E-4 ID:SGTC_458|Compound:nppb|FD-Score:3.47|P-value:4.60E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:-3.66|P-value:1.04E-4 ID:SGTC_758|Compound:0868-0029|FD-Score:-4.23|P-value:9.68E-6 ID:SGTC_893|Compound:0958-0369|FD-Score:-3.37|P-value:3.11E-4 ID:SGTC_952|Compound:1185-0122|FD-Score:3.34|P-value:7.13E-4 ID:SGTC_1409|Compound:3909-8004|FD-Score:4.17|P-value:3.12E-5 ID:SGTC_1412|Compound:3909-8563|FD-Score:3.35|P-value:7.05E-4 ID:SGTC_1420|Compound:4'-methoxyflavone|FD-Score:4.09|P-value:4.42E-5 ID:SGTC_1429|Compound:4049-0205|FD-Score:3.74|P-value:1.73E-4 ID:SGTC_1480|Compound:1070-0044|FD-Score:3.83|P-value:1.21E-4 ID:SGTC_1513|Compound:0337-0559|FD-Score:3.39|P-value:6.16E-4 ID:SGTC_1571|Compound:st070080|FD-Score:3.79|P-value:1.44E-4 ID:SGTC_1573|Compound:oleylamine|FD-Score:-3.78|P-value:6.63E-5 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:4.98|P-value:7.88E-7 ID:SGTC_1856|Compound:st057054|FD-Score:-3.68|P-value:9.66E-5 ID:SGTC_1868|Compound:st057644|FD-Score:3.95|P-value:7.59E-5 ID:SGTC_1911|Compound:st060208|FD-Score:4.22|P-value:2.49E-5 ID:SGTC_1915|Compound:st067105|FD-Score:3.96|P-value:7.36E-5 ID:SGTC_1944|Compound:st076371|FD-Score:4.38|P-value:1.29E-5 ID:SGTC_1953|Compound:st076800|FD-Score:4.38|P-value:1.29E-5 ID:SGTC_1963|Compound:st076565|FD-Score:3.73|P-value:1.76E-4 ID:SGTC_1968|Compound:st069590|FD-Score:4.08|P-value:4.52E-5 ID:SGTC_1982|Compound:st072467|FD-Score:4.22|P-value:2.58E-5 ID:SGTC_2080|Compound:5212524|FD-Score:3.32|P-value:7.71E-4 ID:SGTC_2123|Compound:5259487|FD-Score:3.36|P-value:6.70E-4 ID:SGTC_2128|Compound:5255449|FD-Score:4.95|P-value:8.94E-7 ID:SGTC_2219|Compound:9098279|FD-Score:-3.19|P-value:5.77E-4 ID:SGTC_2303|Compound:7774051|FD-Score:-3.18|P-value:6.00E-4 ID:SGTC_2336|Compound:9001819|FD-Score:4.96|P-value:8.84E-7 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:-3.97|P-value:2.99E-5 ID:SGTC_2458|Compound:5808907|FD-Score:-3.36|P-value:3.18E-4 ID:SGTC_2460|Compound:5552655|FD-Score:3.85|P-value:1.11E-4 ID:SGTC_2464|Compound:5717415|FD-Score:-3.04|P-value:9.83E-4 ID:SGTC_2466|Compound:5983409|FD-Score:3.74|P-value:1.73E-4 ID:SGTC_2467|Compound:5474540|FD-Score:-5.60|P-value:9.62E-9 ID:SGTC_2470|Compound:5664817|FD-Score:3.54|P-value:3.63E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:4.72|P-value:2.77E-6 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:4.31|P-value:1.76E-5 ID:SGTC_2601|Compound:securinine|FD-Score:4.55|P-value:6.11E-6 ID:SGTC_2689|Compound:tacrolimus|FD-Score:4.51|P-value:7.28E-6 ID:SGTC_2830|Compound:7992007|FD-Score:3.61|P-value:2.73E-4 ID:SGTC_2834|Compound:9001755|FD-Score:4.24|P-value:2.35E-5 ID:SGTC_2875|Compound:9039966|FD-Score:3.36|P-value:6.63E-4 ID:SGTC_2882|Compound:9058044|FD-Score:3.36|P-value:6.79E-4 ID:SGTC_2913|Compound:7966556|FD-Score:-3.16|P-value:6.55E-4 ID:SGTC_2922|Compound:7999257|FD-Score:3.69|P-value:2.08E-4 ID:SGTC_2926|Compound:9010726|FD-Score:-3.34|P-value:3.42E-4 ID:SGTC_3052|Compound:9092717|FD-Score:3.29|P-value:8.64E-4 ID:SGTC_3093|Compound:9117344|FD-Score:4.35|P-value:1.46E-5 ID:SGTC_3097|Compound:9117246|FD-Score:3.71|P-value:1.91E-4 ID:SGTC_3131|Compound:9127911|FD-Score:5.02|P-value:6.33E-7 ID:SGTC_3188|Compound:9110789|FD-Score:3.25|P-value:9.84E-4 ID:SGTC_3218|Compound:9131285|FD-Score:3.26|P-value:9.58E-4 ID:SGTC_3331|Compound:9141060|FD-Score:3.94|P-value:7.77E-5 ID:SGTC_3334|Compound:9142812|FD-Score:3.31|P-value:8.03E-4

Top fitness defect scores for YNL296W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3131 9127911 5.02 49.5 µM 6.33E-7 7 2.53E-7
2 SGTC_1575 cyproterone acetate 4.98 48.0 µM 7.88E-7 8 3.19E-7
3 SGTC_2336 9001819 4.96 198.7 µM 8.84E-7 heme biosynthesis & mitochondrial translocase 21 3.60E-7
4 SGTC_2128 5255449 4.95 168.5 µM 8.94E-7 13 3.64E-7
5 SGTC_2592 piperlongumine 4.72 83.3 µM 2.77E-6 TSC3-RPN4 14 1.19E-6
6 SGTC_2601 securinine 4.55 100.0 µM 6.11E-6 17 2.74E-6
7 SGTC_2689 tacrolimus 4.51 100.0 µM 7.28E-6 32 3.30E-6
8 SGTC_1953 st076800 4.38 12.1 µM 1.29E-5 38 6.03E-6
9 SGTC_1944 st076371 4.38 56.9 µM 1.29E-5 15 6.04E-6
10 SGTC_3093 9117344 4.35 49.5 µM 1.46E-5 60S ribosome export 9 6.88E-6
11 SGTC_2597 laccaic acid a 4.31 100.0 µM 1.76E-5 28 8.34E-6
12 SGTC_2834 9001755 4.24 58.4 µM 2.35E-5 26 1.13E-5
13 SGTC_1911 st060208 4.22 74.3 µM 2.49E-5 28 1.21E-5
14 SGTC_1982 st072467 4.22 71.9 µM 2.58E-5 30 1.25E-5
15 SGTC_1409 3909-8004 4.17 61.9 µM 3.12E-5 13 1.53E-5
16 SGTC_1420 4'-methoxyflavone 4.09 42.0 µM 4.42E-5 14 2.20E-5
17 SGTC_1968 st069590 4.08 43.6 µM 4.52E-5 14 2.25E-5
18 SGTC_50 0929-0078 3.97 56.4 µM 6.95E-5 44 3.55E-5
19 SGTC_1915 st067105 3.96 30.6 µM 7.36E-5 RNA pol III & RNase P/MRP 43 3.77E-5
20 SGTC_1868 st057644 3.95 42.7 µM 7.59E-5 37 3.89E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.117 9.22E-12 YML025C YML6 het Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins
0.104 1.28E-9 YIL009W FAA3 hom Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.092 8.47E-8 YLR317W_d YLR317W_d het Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C
0.084 1.03E-6 YLR248W RCK2 hom Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication
0.084 1.10E-6 YPL083C SEN54 het Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p
0.084 1.28E-6 YML029W USA1 hom Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p
0.079 5.00E-6 YPL222W_p FMP40_p hom Putative protein of unknown function; proposed to be involved in responding to environmental stresses; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.079 5.06E-6 YNL194C YNL194C hom Integral membrane protein required for sporulation and plasma membrane sphingolipid content; has sequence similarity to SUR7 and FMP45; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; GFP-fusion protein is more abundant at MCCs (membrane compartment occupied by Can1) in the presence of glycerol and oleate
0.078 5.62E-6 YJL127C SPT10 hom Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box
0.076 1.13E-5 YHR134W WSS1 hom Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response
0.071 3.90E-5 YDR225W HTA1 hom Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p
0.070 4.52E-5 YHL045W_d YHL045W_d hom Putative protein of unknown function; not an essential gene
0.069 5.85E-5 YPR053C_d YPR053C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene NHP6A/YPR052C
0.069 6.18E-5 YMR205C PFK2 hom Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes
0.069 6.38E-5 YHR107C CDC12 het Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells