YNL299W / TRF5

Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL299W / TRF5

Click on Significant Values for Screen Details ID:SGTC_42|Compound:0133-0086|FD-Score:-3.79|P-value:1.87E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:7.32|P-value:2.97E-12 ID:SGTC_157|Compound:1348-1695|FD-Score:4.09|P-value:5.94E-5 ID:SGTC_306|Compound:1672-3436|FD-Score:3.42|P-value:6.50E-4 ID:SGTC_432|Compound:NS 1619|FD-Score:3.84|P-value:1.54E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:4.81|P-value:3.10E-6 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:4.46|P-value:1.40E-5 ID:SGTC_508|Compound:roscovitine|FD-Score:3.45|P-value:5.88E-4 ID:SGTC_537|Compound:1334-0398|FD-Score:3.85|P-value:1.48E-4 ID:SGTC_593|Compound:1203-0218|FD-Score:3.35|P-value:8.13E-4 ID:SGTC_597|Compound:k018-0003|FD-Score:-4.77|P-value:3.79E-6 ID:SGTC_602|Compound:3130-1533|FD-Score:3.51|P-value:4.85E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:4.82|P-value:2.97E-6 ID:SGTC_642|Compound:0335-0876|FD-Score:3.79|P-value:1.86E-4 ID:SGTC_676|Compound:falcarinol|FD-Score:3.46|P-value:5.69E-4 ID:SGTC_679|Compound:0422-0091|FD-Score:-3.96|P-value:9.98E-5 ID:SGTC_689|Compound:0833-0039|FD-Score:6.37|P-value:1.09E-9 ID:SGTC_788|Compound:1182-0090|FD-Score:3.82|P-value:1.65E-4 ID:SGTC_843|Compound:0310-0051|FD-Score:3.37|P-value:7.61E-4 ID:SGTC_845|Compound:0330-0138|FD-Score:3.46|P-value:5.72E-4 ID:SGTC_872|Compound:0588-0139|FD-Score:-3.34|P-value:8.61E-4 ID:SGTC_899|Compound:1083-0020|FD-Score:4.58|P-value:8.20E-6 ID:SGTC_978|Compound:1416-0512|FD-Score:3.49|P-value:5.20E-4 ID:SGTC_1146|Compound:3579-1023|FD-Score:4.80|P-value:3.19E-6 ID:SGTC_1185|Compound:1319-0113|FD-Score:-3.96|P-value:1.00E-4 ID:SGTC_1380|Compound:2291-0730|FD-Score:3.50|P-value:5.05E-4 ID:SGTC_1404|Compound:3909-7820|FD-Score:3.53|P-value:4.56E-4 ID:SGTC_1588|Compound:farnesol|FD-Score:3.45|P-value:5.86E-4 ID:SGTC_1721|Compound:st082152|FD-Score:3.49|P-value:5.09E-4 ID:SGTC_1797|Compound:st048695|FD-Score:3.33|P-value:8.70E-4 ID:SGTC_2023|Compound:5144124|FD-Score:4.67|P-value:5.66E-6 ID:SGTC_2273|Compound:7815444|FD-Score:3.44|P-value:6.03E-4 ID:SGTC_2289|Compound:7946404|FD-Score:3.30|P-value:9.43E-4 ID:SGTC_2324|Compound:6149169|FD-Score:-3.43|P-value:6.37E-4 ID:SGTC_2332|Compound:7982454|FD-Score:-4.04|P-value:7.38E-5 ID:SGTC_2336|Compound:9001819|FD-Score:9.79|P-value:1.84E-20 ID:SGTC_2367|Compound:9070478|FD-Score:-3.70|P-value:2.57E-4 ID:SGTC_2395|Compound:5646831|FD-Score:-3.38|P-value:7.51E-4 ID:SGTC_2455|Compound:5377712|FD-Score:6.04|P-value:6.76E-9 ID:SGTC_2466|Compound:5983409|FD-Score:-3.50|P-value:5.10E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:9.63|P-value:7.14E-20 ID:SGTC_2625|Compound:euphol|FD-Score:-3.46|P-value:5.77E-4 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:-4.51|P-value:1.18E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:-3.57|P-value:3.99E-4 ID:SGTC_2794|Compound:7785382|FD-Score:3.85|P-value:1.46E-4 ID:SGTC_2846|Compound:9008642|FD-Score:4.53|P-value:1.04E-5 ID:SGTC_2971|Compound:9092186|FD-Score:-4.78|P-value:3.59E-6 ID:SGTC_42|Compound:0133-0086|FD-Score:-3.79|P-value:1.87E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:7.32|P-value:2.97E-12 ID:SGTC_157|Compound:1348-1695|FD-Score:4.09|P-value:5.94E-5 ID:SGTC_306|Compound:1672-3436|FD-Score:3.42|P-value:6.50E-4 ID:SGTC_432|Compound:NS 1619|FD-Score:3.84|P-value:1.54E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:4.81|P-value:3.10E-6 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:4.46|P-value:1.40E-5 ID:SGTC_508|Compound:roscovitine|FD-Score:3.45|P-value:5.88E-4 ID:SGTC_537|Compound:1334-0398|FD-Score:3.85|P-value:1.48E-4 ID:SGTC_593|Compound:1203-0218|FD-Score:3.35|P-value:8.13E-4 ID:SGTC_597|Compound:k018-0003|FD-Score:-4.77|P-value:3.79E-6 ID:SGTC_602|Compound:3130-1533|FD-Score:3.51|P-value:4.85E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:4.82|P-value:2.97E-6 ID:SGTC_642|Compound:0335-0876|FD-Score:3.79|P-value:1.86E-4 ID:SGTC_676|Compound:falcarinol|FD-Score:3.46|P-value:5.69E-4 ID:SGTC_679|Compound:0422-0091|FD-Score:-3.96|P-value:9.98E-5 ID:SGTC_689|Compound:0833-0039|FD-Score:6.37|P-value:1.09E-9 ID:SGTC_788|Compound:1182-0090|FD-Score:3.82|P-value:1.65E-4 ID:SGTC_843|Compound:0310-0051|FD-Score:3.37|P-value:7.61E-4 ID:SGTC_845|Compound:0330-0138|FD-Score:3.46|P-value:5.72E-4 ID:SGTC_872|Compound:0588-0139|FD-Score:-3.34|P-value:8.61E-4 ID:SGTC_899|Compound:1083-0020|FD-Score:4.58|P-value:8.20E-6 ID:SGTC_978|Compound:1416-0512|FD-Score:3.49|P-value:5.20E-4 ID:SGTC_1146|Compound:3579-1023|FD-Score:4.80|P-value:3.19E-6 ID:SGTC_1185|Compound:1319-0113|FD-Score:-3.96|P-value:1.00E-4 ID:SGTC_1380|Compound:2291-0730|FD-Score:3.50|P-value:5.05E-4 ID:SGTC_1404|Compound:3909-7820|FD-Score:3.53|P-value:4.56E-4 ID:SGTC_1588|Compound:farnesol|FD-Score:3.45|P-value:5.86E-4 ID:SGTC_1721|Compound:st082152|FD-Score:3.49|P-value:5.09E-4 ID:SGTC_1797|Compound:st048695|FD-Score:3.33|P-value:8.70E-4 ID:SGTC_2023|Compound:5144124|FD-Score:4.67|P-value:5.66E-6 ID:SGTC_2273|Compound:7815444|FD-Score:3.44|P-value:6.03E-4 ID:SGTC_2289|Compound:7946404|FD-Score:3.30|P-value:9.43E-4 ID:SGTC_2324|Compound:6149169|FD-Score:-3.43|P-value:6.37E-4 ID:SGTC_2332|Compound:7982454|FD-Score:-4.04|P-value:7.38E-5 ID:SGTC_2336|Compound:9001819|FD-Score:9.79|P-value:1.84E-20 ID:SGTC_2367|Compound:9070478|FD-Score:-3.70|P-value:2.57E-4 ID:SGTC_2395|Compound:5646831|FD-Score:-3.38|P-value:7.51E-4 ID:SGTC_2455|Compound:5377712|FD-Score:6.04|P-value:6.76E-9 ID:SGTC_2466|Compound:5983409|FD-Score:-3.50|P-value:5.10E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:9.63|P-value:7.14E-20 ID:SGTC_2625|Compound:euphol|FD-Score:-3.46|P-value:5.77E-4 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:-4.51|P-value:1.18E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:-3.57|P-value:3.99E-4 ID:SGTC_2794|Compound:7785382|FD-Score:3.85|P-value:1.46E-4 ID:SGTC_2846|Compound:9008642|FD-Score:4.53|P-value:1.04E-5 ID:SGTC_2971|Compound:9092186|FD-Score:-4.78|P-value:3.59E-6

Top fitness defect scores for YNL299W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2336 9001819 9.79 198.7 µM 1.84E-20 heme biosynthesis & mitochondrial translocase 1 6.29E-23
2 SGTC_2582 agaric acid 9.63 100.0 µM 7.14E-20 redox potentiating 75 2.92E-22
3 SGTC_58 0957-0393 7.32 9.0 µM 2.97E-12 cell wall 153 1.21E-13
4 SGTC_689 0833-0039 6.37 98.0 µM 1.09E-9 ergosterol depletion effects on membrane 13 9.58E-11
5 SGTC_2455 5377712 6.04 9.8 µM 6.76E-9 6 7.55E-10
6 SGTC_610 4215-0184 4.82 155.0 µM 2.97E-6 exosome 2 7.24E-7
7 SGTC_451 chloral hydrate 4.81 100.0 µM 3.10E-6 29 7.62E-7
8 SGTC_1146 3579-1023 4.80 7.0 µM 3.19E-6 5 7.86E-7
9 SGTC_2023 5144124 4.67 124.0 µM 5.66E-6 14 1.50E-6
10 SGTC_899 1083-0020 4.58 478.0 µM 8.20E-6 14 2.28E-6
11 SGTC_2846 9008642 4.53 19.5 µM 1.04E-5 13 2.99E-6
12 SGTC_457 grayanotoxin iii 4.46 135.0 µM 1.40E-5 46 4.15E-6
13 SGTC_157 1348-1695 4.09 65.8 µM 5.94E-5 iron homeostasis 64 2.12E-5
14 SGTC_2794 7785382 3.85 27.3 µM 1.46E-4 40 5.83E-5
15 SGTC_537 1334-0398 3.85 209.0 µM 1.48E-4 30 5.92E-5
16 SGTC_432 NS 1619 3.84 61.3 µM 1.54E-4 32 6.19E-5
17 SGTC_788 1182-0090 3.82 188.0 µM 1.65E-4 endomembrane recycling 82 6.72E-5
18 SGTC_642 0335-0876 3.79 158.0 µM 1.86E-4 ubiquinone biosynthesis & proteasome 46 7.64E-5
19 SGTC_1404 3909-7820 3.53 48.3 µM 4.56E-4 60S ribosome export 15 2.10E-4
20 SGTC_602 3130-1533 3.51 38.1 µM 4.85E-4 30 2.25E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.266 2.47E-55 YML103C NUP188 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188
0.224 2.62E-39 YNL215W IES2 hom Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress
0.210 7.38E-35 YNL106C INP52 hom Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain
0.206 1.37E-33 YBR082C UBC4 hom Ubiquitin-conjugating enzyme (E2); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication
0.199 2.52E-31 YEL007W MIT1 hom Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1
0.194 8.33E-30 YLR238W FAR10 hom Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate
0.189 2.91E-28 YGL178W MPT5 hom mRNA-binding protein of the PUF family; binds to the 3' UTR of specific mRNAs, including those involved in mating type switching, cell wall integrity, chronological lifespan, chromatin modification, and spindle pole body architecture; recruits the CCR4-NOT deadenylase complex to mRNAs along with Dhh1p and Dcp1p to promote deadenylation, decapping, and decay; also interacts with the Caf20p translational initiation repressor, affecting its mRNA target specificity
0.189 2.99E-28 YOR049C RSB1 hom Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane
0.187 7.78E-28 YIL111W COX5B hom Subunit Vb of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Bp is predominantly expressed during anaerobic growth while its isoform Va (Cox5Ap) is expressed during aerobic growth; COX5B has a paralog, COX5A, that arose from the whole genome duplication
0.181 4.13E-26 YNL027W CRZ1 hom Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress
0.180 6.96E-26 YML102W CAC2 hom Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription
0.179 1.35E-25 YIL160C POT1 hom 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
0.176 7.77E-25 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.176 7.93E-25 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.176 9.18E-25 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids