YNL312W / RFA2

Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YNL312W / RFA2

Click on Significant Values for Screen Details ID:SGTC_4|Compound:0960-0234|FD-Score:2.65|P-value:8.27E-4 ID:SGTC_71|Compound:0417-1750|FD-Score:-3.23|P-value:6.25E-5 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:2.74|P-value:5.81E-4 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:3.01|P-value:1.74E-4 ID:SGTC_311|Compound:1315-0136|FD-Score:-2.94|P-value:2.44E-4 ID:SGTC_452|Compound:diindolylmethane|FD-Score:3.32|P-value:4.03E-5 ID:SGTC_495|Compound:decylubiquinone|FD-Score:3.32|P-value:4.08E-5 ID:SGTC_504|Compound:lovastatin|FD-Score:2.65|P-value:8.46E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:-2.63|P-value:9.17E-4 ID:SGTC_530|Compound:4358-1714|FD-Score:3.70|P-value:5.53E-6 ID:SGTC_549|Compound:1661-1316|FD-Score:2.99|P-value:1.94E-4 ID:SGTC_552|Compound:k242-0005|FD-Score:4.14|P-value:4.46E-7 ID:SGTC_618|Compound:k072-0232|FD-Score:3.14|P-value:9.97E-5 ID:SGTC_737|Compound:1950-0117|FD-Score:3.01|P-value:1.80E-4 ID:SGTC_739|Compound:1486-1183|FD-Score:2.96|P-value:2.18E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-5.33|P-value:1.29E-10 ID:SGTC_780|Compound:0109-0199|FD-Score:2.97|P-value:2.17E-4 ID:SGTC_784|Compound:4579-0127|FD-Score:2.79|P-value:4.63E-4 ID:SGTC_1192|Compound:1488-0095|FD-Score:2.70|P-value:6.77E-4 ID:SGTC_1287|Compound:0971-0001|FD-Score:-2.68|P-value:7.50E-4 ID:SGTC_1716|Compound:st033144|FD-Score:2.83|P-value:3.94E-4 ID:SGTC_2255|Compound:7941087|FD-Score:2.76|P-value:5.34E-4 ID:SGTC_2439|Compound:5492030|FD-Score:2.61|P-value:9.86E-4 ID:SGTC_2447|Compound:5741742|FD-Score:4.43|P-value:7.48E-8 ID:SGTC_2755|Compound:lomerizine|FD-Score:-4.36|P-value:1.12E-7 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:-3.99|P-value:1.13E-6 ID:SGTC_2760|Compound:etidronate|FD-Score:-3.52|P-value:1.50E-5 ID:SGTC_2796|Compound:7949634|FD-Score:-3.69|P-value:5.85E-6 ID:SGTC_2935|Compound:9038016|FD-Score:3.20|P-value:7.44E-5 ID:SGTC_2945|Compound:9067681|FD-Score:-2.80|P-value:4.48E-4 ID:SGTC_2998|Compound:9066762|FD-Score:3.04|P-value:1.58E-4 ID:SGTC_3125|Compound:9127534|FD-Score:3.74|P-value:4.60E-6 ID:SGTC_4|Compound:0960-0234|FD-Score:2.65|P-value:8.27E-4 ID:SGTC_71|Compound:0417-1750|FD-Score:-3.23|P-value:6.25E-5 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:2.74|P-value:5.81E-4 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:3.01|P-value:1.74E-4 ID:SGTC_311|Compound:1315-0136|FD-Score:-2.94|P-value:2.44E-4 ID:SGTC_452|Compound:diindolylmethane|FD-Score:3.32|P-value:4.03E-5 ID:SGTC_495|Compound:decylubiquinone|FD-Score:3.32|P-value:4.08E-5 ID:SGTC_504|Compound:lovastatin|FD-Score:2.65|P-value:8.46E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:-2.63|P-value:9.17E-4 ID:SGTC_530|Compound:4358-1714|FD-Score:3.70|P-value:5.53E-6 ID:SGTC_549|Compound:1661-1316|FD-Score:2.99|P-value:1.94E-4 ID:SGTC_552|Compound:k242-0005|FD-Score:4.14|P-value:4.46E-7 ID:SGTC_618|Compound:k072-0232|FD-Score:3.14|P-value:9.97E-5 ID:SGTC_737|Compound:1950-0117|FD-Score:3.01|P-value:1.80E-4 ID:SGTC_739|Compound:1486-1183|FD-Score:2.96|P-value:2.18E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-5.33|P-value:1.29E-10 ID:SGTC_780|Compound:0109-0199|FD-Score:2.97|P-value:2.17E-4 ID:SGTC_784|Compound:4579-0127|FD-Score:2.79|P-value:4.63E-4 ID:SGTC_1192|Compound:1488-0095|FD-Score:2.70|P-value:6.77E-4 ID:SGTC_1287|Compound:0971-0001|FD-Score:-2.68|P-value:7.50E-4 ID:SGTC_1716|Compound:st033144|FD-Score:2.83|P-value:3.94E-4 ID:SGTC_2255|Compound:7941087|FD-Score:2.76|P-value:5.34E-4 ID:SGTC_2439|Compound:5492030|FD-Score:2.61|P-value:9.86E-4 ID:SGTC_2447|Compound:5741742|FD-Score:4.43|P-value:7.48E-8 ID:SGTC_2755|Compound:lomerizine|FD-Score:-4.36|P-value:1.12E-7 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:-3.99|P-value:1.13E-6 ID:SGTC_2760|Compound:etidronate|FD-Score:-3.52|P-value:1.50E-5 ID:SGTC_2796|Compound:7949634|FD-Score:-3.69|P-value:5.85E-6 ID:SGTC_2935|Compound:9038016|FD-Score:3.20|P-value:7.44E-5 ID:SGTC_2945|Compound:9067681|FD-Score:-2.80|P-value:4.48E-4 ID:SGTC_2998|Compound:9066762|FD-Score:3.04|P-value:1.58E-4 ID:SGTC_3125|Compound:9127534|FD-Score:3.74|P-value:4.60E-6

Top fitness defect scores for YNL312W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2447 5741742 4.43 33.8 µM 7.48E-8 6 4.76E-6 0.22
2 SGTC_552 k242-0005 4.14 476.0 µM 4.46E-7 2 1.72E-5 0.52
3 SGTC_3125 9127534 3.74 49.5 µM 4.60E-6 4 9.27E-5 0.30
4 SGTC_530 4358-1714 3.70 28.0 µM 5.53E-6 9 1.06E-4 0.10
5 SGTC_452 diindolylmethane 3.32 8.0 µM 4.03E-5 RNA processing & uracil transport 9 4.45E-4 0.34
6 SGTC_495 decylubiquinone 3.32 155.0 µM 4.08E-5 copper-dependent oxidative stress 4 4.49E-4 0.18
7 SGTC_2935 9038016 3.20 30.0 µM 7.44E-5 13 6.94E-4 0.02
8 SGTC_618 k072-0232 3.14 33.2 µM 9.97E-5 60S ribosome export 15 8.58E-4 0.02
9 SGTC_2998 9066762 3.04 71.4 µM 1.58E-4 RPP1 & pyrimidine depletion 18 0.00120 0.18
10 SGTC_239 5hr heat shock (37°C) + mitomycin C 3.01 819.4 µM 1.74E-4 heat shock/prefoldin 7 0.00129 0.06
11 SGTC_737 1950-0117 3.01 57.9 µM 1.80E-4 16 0.00132 0.02
12 SGTC_549 1661-1316 2.99 519.0 µM 1.94E-4 17 0.00139 0.03
13 SGTC_780 0109-0199 2.97 192.0 µM 2.17E-4 18 0.00151 0.05
14 SGTC_739 1486-1183 2.96 203.0 µM 2.18E-4 9 0.00152 0.19
15 SGTC_1716 st033144 2.83 30.6 µM 3.94E-4 19 0.00233 0.03
16 SGTC_784 4579-0127 2.79 173.0 µM 4.63E-4 14 0.00262 0.09
17 SGTC_2255 7941087 2.76 197.4 µM 5.34E-4 19 0.00291 0.03
18 SGTC_238 5hr heat shock (37°C) + nsc-638432 2.74 8.9 µM 5.81E-4 heat shock/prefoldin 26 0.00310 0.00
19 SGTC_1192 1488-0095 2.70 21.4 µM 6.77E-4 12 0.00346 0.02
20 SGTC_4 0960-0234 2.65 25.7 µM 8.27E-4 Golgi 26 0.00400 0.00

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.241 2.04E-45 YOR087W YVC1 hom Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock
0.186 1.21E-27 YKL072W STB6 hom Protein that binds Sin3p in a two-hybrid assay
0.172 1.06E-23 YLL012W YEH1 hom Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes
0.158 4.04E-20 YHR003C YHR003C hom Protein of unknown function, localized to the mitochondrial outer membrane
0.156 1.25E-19 YMR111C YMR111C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene; forms nuclear foci upon DNA replication stress
0.151 1.24E-18 YFL009W CDC4 het F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p
0.147 9.04E-18 YOR010C TIR2 hom Putative cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; transcription is induced by cold shock and anaerobiosis
0.139 7.46E-16 YEL004W YEA4 hom Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter required for cell wall chitin synthesis; localized to the ER
0.137 1.28E-15 YLR016C PML1 hom Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p
0.129 5.95E-14 YLR223C IFH1 het Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing
0.128 1.17E-13 YOL141W PPM2 hom AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p
0.126 2.01E-13 YBR118W TEF2 hom Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication
0.126 2.05E-13 YOR083W WHI5 hom Repressor of G1 transcription that binds to SCB binding factor (SBF) at SCB target promoters in early G1; phosphorylation of Whi5p by the CDK, Cln3p/Cdc28p relieves repression and promoter binding by Whi5; periodically expressed in G1
0.126 2.96E-13 YCR072C RSA4 het WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus
0.125 3.92E-13 YDR534C FIT1 hom Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall