YNL317W / PFS2

Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex

Zygosity: Heterozygous strain
fixedexpanded
Profile for YNL317W / PFS2

Click on Significant Values for Screen Details ID:SGTC_17|Compound:0960-0276|FD-Score:3.44|P-value:8.79E-4 ID:SGTC_66|Compound:0109-0187|FD-Score:-4.11|P-value:7.08E-5 ID:SGTC_125|Compound:0133-0007|FD-Score:4.92|P-value:3.76E-6 ID:SGTC_126|Compound:0417-1624|FD-Score:3.70|P-value:3.83E-4 ID:SGTC_176|Compound:1486-1328|FD-Score:3.91|P-value:1.91E-4 ID:SGTC_370|Compound:0180-0345|FD-Score:3.90|P-value:1.95E-4 ID:SGTC_386|Compound:1509-0011|FD-Score:-4.00|P-value:1.06E-4 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:3.80|P-value:2.75E-4 ID:SGTC_470|Compound:aa-861|FD-Score:6.03|P-value:1.91E-8 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:6.09|P-value:1.38E-8 ID:SGTC_512|Compound:prima-1|FD-Score:4.84|P-value:5.32E-6 ID:SGTC_556|Compound:4058-0063|FD-Score:3.64|P-value:4.72E-4 ID:SGTC_578|Compound:3460-0051|FD-Score:3.48|P-value:7.77E-4 ID:SGTC_587|Compound:k089-0003|FD-Score:-3.53|P-value:5.45E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:3.91|P-value:1.89E-4 ID:SGTC_642|Compound:0335-0876|FD-Score:3.46|P-value:8.34E-4 ID:SGTC_679|Compound:0422-0091|FD-Score:3.42|P-value:9.38E-4 ID:SGTC_700|Compound:3453-2283|FD-Score:4.98|P-value:2.89E-6 ID:SGTC_711|Compound:k292-0785|FD-Score:5.17|P-value:1.22E-6 ID:SGTC_717|Compound:1532-0246|FD-Score:4.82|P-value:5.66E-6 ID:SGTC_720|Compound:r052-1644|FD-Score:3.53|P-value:6.73E-4 ID:SGTC_726|Compound:3448-4391|FD-Score:-4.53|P-value:1.41E-5 ID:SGTC_858|Compound:0438-0351|FD-Score:3.50|P-value:7.39E-4 ID:SGTC_867|Compound:0518-0118|FD-Score:4.50|P-value:2.10E-5 ID:SGTC_926|Compound:2268-1401|FD-Score:3.96|P-value:1.59E-4 ID:SGTC_1128|Compound:3448-9350|FD-Score:4.10|P-value:9.68E-5 ID:SGTC_1239|Compound:0337-0584|FD-Score:-9.23|P-value:9.50E-18 ID:SGTC_1240|Compound:0356-0016|FD-Score:-4.45|P-value:1.99E-5 ID:SGTC_1242|Compound:0402-0103|FD-Score:3.40|P-value:0.00100 ID:SGTC_1249|Compound:0481-0015|FD-Score:-7.20|P-value:1.61E-11 ID:SGTC_1251|Compound:0490-5699|FD-Score:-3.50|P-value:6.02E-4 ID:SGTC_1265|Compound:0819-1001|FD-Score:-3.46|P-value:6.68E-4 ID:SGTC_1415|Compound:3966-0071|FD-Score:3.73|P-value:3.49E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:-3.89|P-value:1.61E-4 ID:SGTC_1732|Compound:st034308|FD-Score:3.98|P-value:1.48E-4 ID:SGTC_1871|Compound:st057448|FD-Score:-3.53|P-value:5.44E-4 ID:SGTC_1920|Compound:st075728|FD-Score:-3.41|P-value:7.98E-4 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:3.50|P-value:7.33E-4 ID:SGTC_2546|Compound:rhodinyl acetate|FD-Score:-3.97|P-value:1.22E-4 ID:SGTC_2569|Compound:tetrandrine|FD-Score:3.50|P-value:7.28E-4 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:4.26|P-value:5.31E-5 ID:SGTC_2776|Compound:terbinafine|FD-Score:3.50|P-value:7.32E-4 ID:SGTC_2805|Compound:7950783|FD-Score:-3.61|P-value:4.15E-4 ID:SGTC_2853|Compound:9015042|FD-Score:5.21|P-value:1.01E-6 ID:SGTC_2932|Compound:9016711|FD-Score:4.63|P-value:1.26E-5 ID:SGTC_3040|Compound:9091894|FD-Score:5.12|P-value:1.56E-6 ID:SGTC_3067|Compound:9117431|FD-Score:3.46|P-value:8.43E-4 ID:SGTC_3129|Compound:9127257|FD-Score:4.29|P-value:4.79E-5 ID:SGTC_3149|Compound:9097855|FD-Score:5.57|P-value:1.91E-7 ID:SGTC_17|Compound:0960-0276|FD-Score:3.44|P-value:8.79E-4 ID:SGTC_66|Compound:0109-0187|FD-Score:-4.11|P-value:7.08E-5 ID:SGTC_125|Compound:0133-0007|FD-Score:4.92|P-value:3.76E-6 ID:SGTC_126|Compound:0417-1624|FD-Score:3.70|P-value:3.83E-4 ID:SGTC_176|Compound:1486-1328|FD-Score:3.91|P-value:1.91E-4 ID:SGTC_370|Compound:0180-0345|FD-Score:3.90|P-value:1.95E-4 ID:SGTC_386|Compound:1509-0011|FD-Score:-4.00|P-value:1.06E-4 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:3.80|P-value:2.75E-4 ID:SGTC_470|Compound:aa-861|FD-Score:6.03|P-value:1.91E-8 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:6.09|P-value:1.38E-8 ID:SGTC_512|Compound:prima-1|FD-Score:4.84|P-value:5.32E-6 ID:SGTC_556|Compound:4058-0063|FD-Score:3.64|P-value:4.72E-4 ID:SGTC_578|Compound:3460-0051|FD-Score:3.48|P-value:7.77E-4 ID:SGTC_587|Compound:k089-0003|FD-Score:-3.53|P-value:5.45E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:3.91|P-value:1.89E-4 ID:SGTC_642|Compound:0335-0876|FD-Score:3.46|P-value:8.34E-4 ID:SGTC_679|Compound:0422-0091|FD-Score:3.42|P-value:9.38E-4 ID:SGTC_700|Compound:3453-2283|FD-Score:4.98|P-value:2.89E-6 ID:SGTC_711|Compound:k292-0785|FD-Score:5.17|P-value:1.22E-6 ID:SGTC_717|Compound:1532-0246|FD-Score:4.82|P-value:5.66E-6 ID:SGTC_720|Compound:r052-1644|FD-Score:3.53|P-value:6.73E-4 ID:SGTC_726|Compound:3448-4391|FD-Score:-4.53|P-value:1.41E-5 ID:SGTC_858|Compound:0438-0351|FD-Score:3.50|P-value:7.39E-4 ID:SGTC_867|Compound:0518-0118|FD-Score:4.50|P-value:2.10E-5 ID:SGTC_926|Compound:2268-1401|FD-Score:3.96|P-value:1.59E-4 ID:SGTC_1128|Compound:3448-9350|FD-Score:4.10|P-value:9.68E-5 ID:SGTC_1239|Compound:0337-0584|FD-Score:-9.23|P-value:9.50E-18 ID:SGTC_1240|Compound:0356-0016|FD-Score:-4.45|P-value:1.99E-5 ID:SGTC_1242|Compound:0402-0103|FD-Score:3.40|P-value:0.00100 ID:SGTC_1249|Compound:0481-0015|FD-Score:-7.20|P-value:1.61E-11 ID:SGTC_1251|Compound:0490-5699|FD-Score:-3.50|P-value:6.02E-4 ID:SGTC_1265|Compound:0819-1001|FD-Score:-3.46|P-value:6.68E-4 ID:SGTC_1415|Compound:3966-0071|FD-Score:3.73|P-value:3.49E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:-3.89|P-value:1.61E-4 ID:SGTC_1732|Compound:st034308|FD-Score:3.98|P-value:1.48E-4 ID:SGTC_1871|Compound:st057448|FD-Score:-3.53|P-value:5.44E-4 ID:SGTC_1920|Compound:st075728|FD-Score:-3.41|P-value:7.98E-4 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:3.50|P-value:7.33E-4 ID:SGTC_2546|Compound:rhodinyl acetate|FD-Score:-3.97|P-value:1.22E-4 ID:SGTC_2569|Compound:tetrandrine|FD-Score:3.50|P-value:7.28E-4 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:4.26|P-value:5.31E-5 ID:SGTC_2776|Compound:terbinafine|FD-Score:3.50|P-value:7.32E-4 ID:SGTC_2805|Compound:7950783|FD-Score:-3.61|P-value:4.15E-4 ID:SGTC_2853|Compound:9015042|FD-Score:5.21|P-value:1.01E-6 ID:SGTC_2932|Compound:9016711|FD-Score:4.63|P-value:1.26E-5 ID:SGTC_3040|Compound:9091894|FD-Score:5.12|P-value:1.56E-6 ID:SGTC_3067|Compound:9117431|FD-Score:3.46|P-value:8.43E-4 ID:SGTC_3129|Compound:9127257|FD-Score:4.29|P-value:4.79E-5 ID:SGTC_3149|Compound:9097855|FD-Score:5.57|P-value:1.91E-7

Top fitness defect scores for YNL317W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_471 1-acyl-paf 6.09 4.0 µM 1.38E-8 DNA intercalators 8 5.58E-10 0.50
2 SGTC_470 aa-861 6.03 153.0 µM 1.91E-8 sphingolipid biosynthesis & PDR1 4 8.25E-10 0.18
3 SGTC_3149 9097855 5.57 49.5 µM 1.91E-7 Golgi 3 1.27E-8 0.43
4 SGTC_2853 9015042 5.21 58.4 µM 1.01E-6 3 9.25E-8 0.96
5 SGTC_711 k292-0785 5.17 179.0 µM 1.22E-6 DNA intercalators 2 1.15E-7 0.34
6 SGTC_3040 9091894 5.12 49.5 µM 1.56E-6 DNA intercalators 6 1.55E-7 0.10
7 SGTC_700 3453-2283 4.98 142.0 µM 2.89E-6 amide catabolism 3 3.22E-7 0.66
8 SGTC_125 0133-0007 4.92 11.8 µM 3.76E-6 DNA intercalators 3 4.40E-7 0.25
9 SGTC_512 prima-1 4.84 270.0 µM 5.32E-6 DNA intercalators 7 6.64E-7 0.06
10 SGTC_717 1532-0246 4.82 73.2 µM 5.66E-6 Golgi 2 7.15E-7 0.21
11 SGTC_2932 9016711 4.63 44.5 µM 1.26E-5 6 1.85E-6 0.46
12 SGTC_867 0518-0118 4.50 155.0 µM 2.10E-5 DNA intercalators 10 3.39E-6 0.00
13 SGTC_3129 9127257 4.29 49.5 µM 4.79E-5 4 9.05E-6 0.13
14 SGTC_2604 4'-hydroxychalcone 4.26 11.3 µM 5.31E-5 11 1.02E-5 0.17
15 SGTC_1128 3448-9350 4.10 235.0 µM 9.68E-5 5 2.08E-5 0.09
16 SGTC_1732 st034308 3.98 51.8 µM 1.48E-4 9 3.46E-5 0.03
17 SGTC_926 2268-1401 3.96 59.4 µM 1.59E-4 TSC3-RPN4 9 3.77E-5 0.05
18 SGTC_633 0437-0093 3.91 97.7 µM 1.89E-4 DNA intercalators 9 4.61E-5 0.10
19 SGTC_176 1486-1328 3.91 72.8 µM 1.91E-4 8 4.67E-5 0.00
20 SGTC_370 0180-0345 3.90 38.7 µM 1.95E-4 DNA intercalators 9 4.78E-5 0.43

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.345 6.89E-94 YMR119W ASI1 hom Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication
0.290 5.50E-66 YER003C PMI40 het Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
0.282 1.72E-62 YDL058W USO1 het Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex
0.261 3.64E-53 YMR208W ERG12 het Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
0.243 2.20E-46 YOR278W HEM4 het Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria
0.231 9.29E-42 YDR458C HEH2 hom Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle
0.222 1.15E-38 YDR060W MAK21 het Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein
0.220 7.95E-38 YGR156W PTI1 het Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64
0.219 1.23E-37 YGL200C EMP24 hom Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles
0.192 4.44E-29 YDR362C TFC6 het One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110
0.178 2.93E-25 YOR294W RRS1 het Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice
0.173 6.80E-24 YGL023C PIB2 hom Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27
0.147 9.15E-18 YKL139W CTK1 hom Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13
0.144 4.72E-17 YHR172W SPC97 het Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
0.112 7.50E-11 YJL025W RRN7 het Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p