YNL323W / LEM3

Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL323W / LEM3

Click on Significant Values for Screen Details ID:SGTC_52|Compound:0329-0193|FD-Score:-5.48|P-value:1.48E-5 ID:SGTC_54|Compound:0159-0055|FD-Score:12.50|P-value:1.04E-20 ID:SGTC_55|Compound:0767-0285|FD-Score:8.74|P-value:6.33E-11 ID:SGTC_58|Compound:0957-0393|FD-Score:-20.00|P-value:3.46E-51 ID:SGTC_65|Compound:0798-0905|FD-Score:4.48|P-value:5.64E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:4.75|P-value:2.73E-4 ID:SGTC_150|Compound:3970-0969|FD-Score:6.72|P-value:4.20E-7 ID:SGTC_164|Compound:4100-3994|FD-Score:5.47|P-value:3.26E-5 ID:SGTC_168|Compound:3970-0862|FD-Score:8.94|P-value:2.37E-11 ID:SGTC_176|Compound:1486-1328|FD-Score:8.89|P-value:3.00E-11 ID:SGTC_187|Compound:1488-2104|FD-Score:4.75|P-value:2.78E-4 ID:SGTC_197|Compound:4544-0043|FD-Score:5.22|P-value:7.00E-5 ID:SGTC_233|Compound:rapamycin|FD-Score:5.28|P-value:5.88E-5 ID:SGTC_234|Compound:nsc-17383|FD-Score:6.44|P-value:1.23E-6 ID:SGTC_255|Compound:tunicamycin|FD-Score:-5.55|P-value:1.18E-5 ID:SGTC_266|Compound:formosanin C|FD-Score:-4.32|P-value:4.64E-4 ID:SGTC_315|Compound:k915-0449|FD-Score:6.09|P-value:4.21E-6 ID:SGTC_391|Compound:gliotoxin|FD-Score:4.98|P-value:1.44E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-9.45|P-value:4.77E-13 ID:SGTC_500|Compound:h-89|FD-Score:5.56|P-value:2.46E-5 ID:SGTC_625|Compound:k213-0058|FD-Score:5.62|P-value:2.02E-5 ID:SGTC_690|Compound:glyoxal|FD-Score:-4.49|P-value:2.93E-4 ID:SGTC_724|Compound:3910-0327|FD-Score:5.03|P-value:1.23E-4 ID:SGTC_748|Compound:0136-0228|FD-Score:8.22|P-value:7.44E-10 ID:SGTC_841|Compound:0251-0215|FD-Score:9.51|P-value:1.26E-12 ID:SGTC_847|Compound:0342-0274|FD-Score:6.22|P-value:2.69E-6 ID:SGTC_859|Compound:0439-0102|FD-Score:7.63|P-value:1.06E-8 ID:SGTC_863|Compound:0466-0191|FD-Score:7.91|P-value:3.10E-9 ID:SGTC_897|Compound:1000-0139|FD-Score:5.67|P-value:1.71E-5 ID:SGTC_922|Compound:1866-0035|FD-Score:6.58|P-value:7.33E-7 ID:SGTC_928|Compound:2764-0428|FD-Score:5.76|P-value:1.31E-5 ID:SGTC_1003|Compound:4049-0218|FD-Score:-4.41|P-value:3.72E-4 ID:SGTC_1104|Compound:tacrolimus|FD-Score:4.32|P-value:8.66E-4 ID:SGTC_1244|Compound:0416-0015|FD-Score:5.31|P-value:5.40E-5 ID:SGTC_1287|Compound:0971-0001|FD-Score:4.39|P-value:7.22E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:5.66|P-value:1.82E-5 ID:SGTC_1369|Compound:1866-0067|FD-Score:8.03|P-value:1.78E-9 ID:SGTC_1377|Compound:2944-0135|FD-Score:6.61|P-value:6.39E-7 ID:SGTC_1464|Compound:k208-0027|FD-Score:4.79|P-value:2.47E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:8.46|P-value:2.43E-10 ID:SGTC_1545|Compound:lovastatin|FD-Score:6.34|P-value:1.72E-6 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:4.44|P-value:6.30E-4 ID:SGTC_1581|Compound:himbacine|FD-Score:5.38|P-value:4.31E-5 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:7.99|P-value:2.18E-9 ID:SGTC_1627|Compound:st003713|FD-Score:8.19|P-value:8.59E-10 ID:SGTC_1666|Compound:st014829|FD-Score:9.59|P-value:8.04E-13 ID:SGTC_1687|Compound:st020778|FD-Score:4.73|P-value:2.93E-4 ID:SGTC_1690|Compound:st023502|FD-Score:5.61|P-value:2.07E-5 ID:SGTC_1728|Compound:st035614|FD-Score:5.00|P-value:1.34E-4 ID:SGTC_1760|Compound:st045453|FD-Score:7.64|P-value:1.02E-8 ID:SGTC_1766|Compound:st045414|FD-Score:-4.81|P-value:1.20E-4 ID:SGTC_1826|Compound:st055655|FD-Score:9.10|P-value:1.06E-11 ID:SGTC_1855|Compound:st056195|FD-Score:4.78|P-value:2.53E-4 ID:SGTC_1860|Compound:st056228|FD-Score:4.65|P-value:3.62E-4 ID:SGTC_1874|Compound:staurosporine|FD-Score:7.39|P-value:2.89E-8 ID:SGTC_1877|Compound:wortmannin|FD-Score:5.28|P-value:5.95E-5 ID:SGTC_1921|Compound:5740136|FD-Score:5.65|P-value:1.86E-5 ID:SGTC_1983|Compound:st071813|FD-Score:5.19|P-value:7.70E-5 ID:SGTC_1988|Compound:st071872|FD-Score:-4.68|P-value:1.71E-4 ID:SGTC_1996|Compound:st072960|FD-Score:-4.17|P-value:6.85E-4 ID:SGTC_2077|Compound:5212015|FD-Score:10.30|P-value:1.83E-14 ID:SGTC_2123|Compound:5259487|FD-Score:4.29|P-value:9.26E-4 ID:SGTC_2306|Compound:7482397|FD-Score:-4.23|P-value:5.95E-4 ID:SGTC_2322|Compound:7413319|FD-Score:4.57|P-value:4.55E-4 ID:SGTC_2350|Compound:9023895|FD-Score:-4.74|P-value:1.46E-4 ID:SGTC_2363|Compound:9053361|FD-Score:4.88|P-value:1.90E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:5.49|P-value:3.04E-5 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:6.55|P-value:8.11E-7 ID:SGTC_2489|Compound:cantharidin|FD-Score:6.14|P-value:3.54E-6 ID:SGTC_2514|Compound:salinomycin|FD-Score:4.87|P-value:1.99E-4 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:7.99|P-value:2.13E-9 ID:SGTC_2601|Compound:securinine|FD-Score:5.98|P-value:6.17E-6 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:8.77|P-value:5.47E-11 ID:SGTC_2645|Compound:abamectin|FD-Score:5.23|P-value:6.86E-5 ID:SGTC_2752|Compound:diclazuril|FD-Score:4.90|P-value:1.82E-4 ID:SGTC_2821|Compound:7996764|FD-Score:4.45|P-value:6.25E-4 ID:SGTC_2852|Compound:9023854|FD-Score:4.40|P-value:6.99E-4 ID:SGTC_2854|Compound:9024559|FD-Score:-8.74|P-value:1.94E-11 ID:SGTC_2856|Compound:9022925|FD-Score:-4.36|P-value:4.19E-4 ID:SGTC_2862|Compound:9027634|FD-Score:5.41|P-value:3.95E-5 ID:SGTC_3025|Compound:9087895|FD-Score:7.07|P-value:1.08E-7 ID:SGTC_3074|Compound:9116018|FD-Score:5.72|P-value:1.47E-5 ID:SGTC_3101|Compound:9120531|FD-Score:4.86|P-value:2.03E-4 ID:SGTC_3174|Compound:9107204|FD-Score:4.41|P-value:6.78E-4 ID:SGTC_3201|Compound:9113999|FD-Score:10.30|P-value:1.32E-14 ID:SGTC_3202|Compound:9113761|FD-Score:6.79|P-value:3.23E-7 ID:SGTC_3321|Compound:9137793|FD-Score:8.08|P-value:1.43E-9 ID:SGTC_3338|Compound:9144300|FD-Score:6.65|P-value:5.56E-7 ID:SGTC_52|Compound:0329-0193|FD-Score:-5.48|P-value:1.48E-5 ID:SGTC_54|Compound:0159-0055|FD-Score:12.50|P-value:1.04E-20 ID:SGTC_55|Compound:0767-0285|FD-Score:8.74|P-value:6.33E-11 ID:SGTC_58|Compound:0957-0393|FD-Score:-20.00|P-value:3.46E-51 ID:SGTC_65|Compound:0798-0905|FD-Score:4.48|P-value:5.64E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:4.75|P-value:2.73E-4 ID:SGTC_150|Compound:3970-0969|FD-Score:6.72|P-value:4.20E-7 ID:SGTC_164|Compound:4100-3994|FD-Score:5.47|P-value:3.26E-5 ID:SGTC_168|Compound:3970-0862|FD-Score:8.94|P-value:2.37E-11 ID:SGTC_176|Compound:1486-1328|FD-Score:8.89|P-value:3.00E-11 ID:SGTC_187|Compound:1488-2104|FD-Score:4.75|P-value:2.78E-4 ID:SGTC_197|Compound:4544-0043|FD-Score:5.22|P-value:7.00E-5 ID:SGTC_233|Compound:rapamycin|FD-Score:5.28|P-value:5.88E-5 ID:SGTC_234|Compound:nsc-17383|FD-Score:6.44|P-value:1.23E-6 ID:SGTC_255|Compound:tunicamycin|FD-Score:-5.55|P-value:1.18E-5 ID:SGTC_266|Compound:formosanin C|FD-Score:-4.32|P-value:4.64E-4 ID:SGTC_315|Compound:k915-0449|FD-Score:6.09|P-value:4.21E-6 ID:SGTC_391|Compound:gliotoxin|FD-Score:4.98|P-value:1.44E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-9.45|P-value:4.77E-13 ID:SGTC_500|Compound:h-89|FD-Score:5.56|P-value:2.46E-5 ID:SGTC_625|Compound:k213-0058|FD-Score:5.62|P-value:2.02E-5 ID:SGTC_690|Compound:glyoxal|FD-Score:-4.49|P-value:2.93E-4 ID:SGTC_724|Compound:3910-0327|FD-Score:5.03|P-value:1.23E-4 ID:SGTC_748|Compound:0136-0228|FD-Score:8.22|P-value:7.44E-10 ID:SGTC_841|Compound:0251-0215|FD-Score:9.51|P-value:1.26E-12 ID:SGTC_847|Compound:0342-0274|FD-Score:6.22|P-value:2.69E-6 ID:SGTC_859|Compound:0439-0102|FD-Score:7.63|P-value:1.06E-8 ID:SGTC_863|Compound:0466-0191|FD-Score:7.91|P-value:3.10E-9 ID:SGTC_897|Compound:1000-0139|FD-Score:5.67|P-value:1.71E-5 ID:SGTC_922|Compound:1866-0035|FD-Score:6.58|P-value:7.33E-7 ID:SGTC_928|Compound:2764-0428|FD-Score:5.76|P-value:1.31E-5 ID:SGTC_1003|Compound:4049-0218|FD-Score:-4.41|P-value:3.72E-4 ID:SGTC_1104|Compound:tacrolimus|FD-Score:4.32|P-value:8.66E-4 ID:SGTC_1244|Compound:0416-0015|FD-Score:5.31|P-value:5.40E-5 ID:SGTC_1287|Compound:0971-0001|FD-Score:4.39|P-value:7.22E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:5.66|P-value:1.82E-5 ID:SGTC_1369|Compound:1866-0067|FD-Score:8.03|P-value:1.78E-9 ID:SGTC_1377|Compound:2944-0135|FD-Score:6.61|P-value:6.39E-7 ID:SGTC_1464|Compound:k208-0027|FD-Score:4.79|P-value:2.47E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:8.46|P-value:2.43E-10 ID:SGTC_1545|Compound:lovastatin|FD-Score:6.34|P-value:1.72E-6 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:4.44|P-value:6.30E-4 ID:SGTC_1581|Compound:himbacine|FD-Score:5.38|P-value:4.31E-5 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:7.99|P-value:2.18E-9 ID:SGTC_1627|Compound:st003713|FD-Score:8.19|P-value:8.59E-10 ID:SGTC_1666|Compound:st014829|FD-Score:9.59|P-value:8.04E-13 ID:SGTC_1687|Compound:st020778|FD-Score:4.73|P-value:2.93E-4 ID:SGTC_1690|Compound:st023502|FD-Score:5.61|P-value:2.07E-5 ID:SGTC_1728|Compound:st035614|FD-Score:5.00|P-value:1.34E-4 ID:SGTC_1760|Compound:st045453|FD-Score:7.64|P-value:1.02E-8 ID:SGTC_1766|Compound:st045414|FD-Score:-4.81|P-value:1.20E-4 ID:SGTC_1826|Compound:st055655|FD-Score:9.10|P-value:1.06E-11 ID:SGTC_1855|Compound:st056195|FD-Score:4.78|P-value:2.53E-4 ID:SGTC_1860|Compound:st056228|FD-Score:4.65|P-value:3.62E-4 ID:SGTC_1874|Compound:staurosporine|FD-Score:7.39|P-value:2.89E-8 ID:SGTC_1877|Compound:wortmannin|FD-Score:5.28|P-value:5.95E-5 ID:SGTC_1921|Compound:5740136|FD-Score:5.65|P-value:1.86E-5 ID:SGTC_1983|Compound:st071813|FD-Score:5.19|P-value:7.70E-5 ID:SGTC_1988|Compound:st071872|FD-Score:-4.68|P-value:1.71E-4 ID:SGTC_1996|Compound:st072960|FD-Score:-4.17|P-value:6.85E-4 ID:SGTC_2077|Compound:5212015|FD-Score:10.30|P-value:1.83E-14 ID:SGTC_2123|Compound:5259487|FD-Score:4.29|P-value:9.26E-4 ID:SGTC_2306|Compound:7482397|FD-Score:-4.23|P-value:5.95E-4 ID:SGTC_2322|Compound:7413319|FD-Score:4.57|P-value:4.55E-4 ID:SGTC_2350|Compound:9023895|FD-Score:-4.74|P-value:1.46E-4 ID:SGTC_2363|Compound:9053361|FD-Score:4.88|P-value:1.90E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:5.49|P-value:3.04E-5 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:6.55|P-value:8.11E-7 ID:SGTC_2489|Compound:cantharidin|FD-Score:6.14|P-value:3.54E-6 ID:SGTC_2514|Compound:salinomycin|FD-Score:4.87|P-value:1.99E-4 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:7.99|P-value:2.13E-9 ID:SGTC_2601|Compound:securinine|FD-Score:5.98|P-value:6.17E-6 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:8.77|P-value:5.47E-11 ID:SGTC_2645|Compound:abamectin|FD-Score:5.23|P-value:6.86E-5 ID:SGTC_2752|Compound:diclazuril|FD-Score:4.90|P-value:1.82E-4 ID:SGTC_2821|Compound:7996764|FD-Score:4.45|P-value:6.25E-4 ID:SGTC_2852|Compound:9023854|FD-Score:4.40|P-value:6.99E-4 ID:SGTC_2854|Compound:9024559|FD-Score:-8.74|P-value:1.94E-11 ID:SGTC_2856|Compound:9022925|FD-Score:-4.36|P-value:4.19E-4 ID:SGTC_2862|Compound:9027634|FD-Score:5.41|P-value:3.95E-5 ID:SGTC_3025|Compound:9087895|FD-Score:7.07|P-value:1.08E-7 ID:SGTC_3074|Compound:9116018|FD-Score:5.72|P-value:1.47E-5 ID:SGTC_3101|Compound:9120531|FD-Score:4.86|P-value:2.03E-4 ID:SGTC_3174|Compound:9107204|FD-Score:4.41|P-value:6.78E-4 ID:SGTC_3201|Compound:9113999|FD-Score:10.30|P-value:1.32E-14 ID:SGTC_3202|Compound:9113761|FD-Score:6.79|P-value:3.23E-7 ID:SGTC_3321|Compound:9137793|FD-Score:8.08|P-value:1.43E-9 ID:SGTC_3338|Compound:9144300|FD-Score:6.65|P-value:5.56E-7

Top fitness defect scores for YNL323W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_54 0159-0055 12.50 9.0 µM 1.04E-20 ERG2 6 2.49E-36
2 SGTC_3201 9113999 10.30 49.5 µM 1.32E-14 2 2.62E-25
3 SGTC_2077 5212015 10.30 200.0 µM 1.83E-14 5 4.71E-25
4 SGTC_1666 st014829 9.59 29.0 µM 8.04E-13 calcium & mitochondrial duress 5 4.35E-22
5 SGTC_841 0251-0215 9.51 91.2 µM 1.26E-12 17 9.72E-22
6 SGTC_1826 st055655 9.10 41.6 µM 1.06E-11 ERG2 7 4.58E-20
7 SGTC_168 3970-0862 8.94 530.5 µM 2.37E-11 15 1.95E-19
8 SGTC_176 1486-1328 8.89 72.8 µM 3.00E-11 9 2.98E-19
9 SGTC_2632 3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone 8.77 100.0 µM 5.47E-11 2 8.83E-19
10 SGTC_55 0767-0285 8.74 6.6 µM 6.33E-11 7 1.15E-18
11 SGTC_1495 4469-0029 8.46 237.0 µM 2.43E-10 22 1.30E-17
12 SGTC_748 0136-0228 8.22 77.9 µM 7.44E-10 redox potentiating 16 9.79E-17
13 SGTC_1627 st003713 8.19 25.6 µM 8.59E-10 calcium & mitochondrial duress 14 1.27E-16
14 SGTC_3321 9137793 8.08 26.8 µM 1.43E-9 cell wall 9 3.19E-16
15 SGTC_1369 1866-0067 8.03 208.0 µM 1.78E-9 RPP1 & pyrimidine depletion 2 4.72E-16
16 SGTC_2572 astragaloside iv 7.99 100.0 µM 2.13E-9 1 6.49E-16
17 SGTC_1597 7-benzyloxygramine 7.99 47.5 µM 2.18E-9 calcium & mitochondrial duress 25 6.77E-16
18 SGTC_863 0466-0191 7.91 129.0 µM 3.10E-9 cell wall 17 1.28E-15
19 SGTC_1760 st045453 7.64 10.4 µM 1.02E-8 NEO1-PIK1 59 1.10E-14
20 SGTC_859 0439-0102 7.63 54.6 µM 1.06E-8 redox potentiating 2 1.18E-14

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.347 3.73E-95 YMR202W ERG2 hom C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
0.329 7.18E-85 YGL012W ERG4 hom C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
0.288 6.99E-65 YKL212W SAC1 hom Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
0.264 2.59E-54 YDL192W ARF1 hom ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
0.213 1.52E-35 YFL025C BST1 hom GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules
0.212 3.55E-35 YIL154C IMP2' hom Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat
0.209 4.91E-34 YGL095C VPS45 hom Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment
0.204 1.47E-32 YOR036W PEP12 hom Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
0.203 2.11E-32 YKL126W YPK1 hom Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
0.201 1.49E-31 YGR285C ZUO1 hom Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p
0.195 7.18E-30 YLR372W SUR4 hom Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis
0.179 1.82E-25 YDR323C PEP7 hom Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
0.176 1.02E-24 YDR495C VPS3 hom Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
0.174 5.86E-24 YMR123W PKR1 hom V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress
0.171 3.35E-23 YLR087C CSF1 hom Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies