YNL324W_d

Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL324W / YNL324W

Click on Significant Values for Screen Details ID:SGTC_31|Compound:0508-1974|FD-Score:-4.37|P-value:5.21E-5 ID:SGTC_53|Compound:0973-0023|FD-Score:-4.43|P-value:4.12E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:14.40|P-value:1.26E-37 ID:SGTC_172|Compound:1112-0046|FD-Score:5.58|P-value:3.67E-7 ID:SGTC_208|Compound:k072-0098|FD-Score:4.08|P-value:1.45E-4 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:3.58|P-value:7.47E-4 ID:SGTC_258|Compound:haloperidol|FD-Score:3.84|P-value:3.29E-4 ID:SGTC_276|Compound:colchicine|FD-Score:-5.24|P-value:1.64E-6 ID:SGTC_309|Compound:k408-0051|FD-Score:-6.35|P-value:8.26E-9 ID:SGTC_310|Compound:1273-0058|FD-Score:5.47|P-value:6.01E-7 ID:SGTC_313|Compound:3474-0040|FD-Score:4.50|P-value:3.25E-5 ID:SGTC_429|Compound:1112-0049|FD-Score:3.65|P-value:6.02E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:-5.97|P-value:5.54E-8 ID:SGTC_516|Compound:3992-2719|FD-Score:-4.15|P-value:1.14E-4 ID:SGTC_537|Compound:1334-0398|FD-Score:3.75|P-value:4.40E-4 ID:SGTC_553|Compound:k007-0256|FD-Score:7.71|P-value:3.71E-12 ID:SGTC_555|Compound:4296-0685|FD-Score:4.09|P-value:1.42E-4 ID:SGTC_701|Compound:4424-0219|FD-Score:-3.74|P-value:4.51E-4 ID:SGTC_705|Compound:1124-0212|FD-Score:3.69|P-value:5.28E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:13.70|P-value:1.43E-34 ID:SGTC_871|Compound:0574-0004|FD-Score:3.65|P-value:5.96E-4 ID:SGTC_887|Compound:0885-0007|FD-Score:4.61|P-value:2.16E-5 ID:SGTC_1097|Compound:itavastatin|FD-Score:3.51|P-value:9.13E-4 ID:SGTC_1308|Compound:1187-1617|FD-Score:5.99|P-value:5.25E-8 ID:SGTC_1343|Compound:1487-0095|FD-Score:5.27|P-value:1.43E-6 ID:SGTC_1357|Compound:1503-1303|FD-Score:3.77|P-value:4.09E-4 ID:SGTC_1379|Compound:2144-0755|FD-Score:4.02|P-value:1.81E-4 ID:SGTC_1648|Compound:st011736|FD-Score:-3.70|P-value:5.00E-4 ID:SGTC_1782|Compound:st039423|FD-Score:-4.34|P-value:5.74E-5 ID:SGTC_1787|Compound:fluconazole|FD-Score:4.14|P-value:1.20E-4 ID:SGTC_1790|Compound:st045711|FD-Score:-3.62|P-value:6.41E-4 ID:SGTC_2107|Compound:5363541|FD-Score:4.85|P-value:8.18E-6 ID:SGTC_2305|Compound:7443522|FD-Score:5.52|P-value:4.71E-7 ID:SGTC_2399|Compound:5884133|FD-Score:3.97|P-value:2.10E-4 ID:SGTC_2415|Compound:st077333|FD-Score:-4.66|P-value:1.70E-5 ID:SGTC_2514|Compound:salinomycin|FD-Score:4.49|P-value:3.30E-5 ID:SGTC_2575|Compound:plumbagin|FD-Score:3.91|P-value:2.62E-4 ID:SGTC_2790|Compound:5423970|FD-Score:4.33|P-value:6.10E-5 ID:SGTC_2884|Compound:9058502|FD-Score:-3.85|P-value:3.13E-4 ID:SGTC_3021|Compound:9087658|FD-Score:4.43|P-value:4.23E-5 ID:SGTC_3071|Compound:9117417|FD-Score:4.33|P-value:5.97E-5 ID:SGTC_3100|Compound:9120536|FD-Score:5.13|P-value:2.59E-6 ID:SGTC_3138|Compound:9097216|FD-Score:-3.98|P-value:2.03E-4 ID:SGTC_3142|Compound:9086498|FD-Score:3.66|P-value:5.73E-4 ID:SGTC_3269|Compound:9137823|FD-Score:3.92|P-value:2.47E-4 ID:SGTC_31|Compound:0508-1974|FD-Score:-4.37|P-value:5.21E-5 ID:SGTC_53|Compound:0973-0023|FD-Score:-4.43|P-value:4.12E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:14.40|P-value:1.26E-37 ID:SGTC_172|Compound:1112-0046|FD-Score:5.58|P-value:3.67E-7 ID:SGTC_208|Compound:k072-0098|FD-Score:4.08|P-value:1.45E-4 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:3.58|P-value:7.47E-4 ID:SGTC_258|Compound:haloperidol|FD-Score:3.84|P-value:3.29E-4 ID:SGTC_276|Compound:colchicine|FD-Score:-5.24|P-value:1.64E-6 ID:SGTC_309|Compound:k408-0051|FD-Score:-6.35|P-value:8.26E-9 ID:SGTC_310|Compound:1273-0058|FD-Score:5.47|P-value:6.01E-7 ID:SGTC_313|Compound:3474-0040|FD-Score:4.50|P-value:3.25E-5 ID:SGTC_429|Compound:1112-0049|FD-Score:3.65|P-value:6.02E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:-5.97|P-value:5.54E-8 ID:SGTC_516|Compound:3992-2719|FD-Score:-4.15|P-value:1.14E-4 ID:SGTC_537|Compound:1334-0398|FD-Score:3.75|P-value:4.40E-4 ID:SGTC_553|Compound:k007-0256|FD-Score:7.71|P-value:3.71E-12 ID:SGTC_555|Compound:4296-0685|FD-Score:4.09|P-value:1.42E-4 ID:SGTC_701|Compound:4424-0219|FD-Score:-3.74|P-value:4.51E-4 ID:SGTC_705|Compound:1124-0212|FD-Score:3.69|P-value:5.28E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:13.70|P-value:1.43E-34 ID:SGTC_871|Compound:0574-0004|FD-Score:3.65|P-value:5.96E-4 ID:SGTC_887|Compound:0885-0007|FD-Score:4.61|P-value:2.16E-5 ID:SGTC_1097|Compound:itavastatin|FD-Score:3.51|P-value:9.13E-4 ID:SGTC_1308|Compound:1187-1617|FD-Score:5.99|P-value:5.25E-8 ID:SGTC_1343|Compound:1487-0095|FD-Score:5.27|P-value:1.43E-6 ID:SGTC_1357|Compound:1503-1303|FD-Score:3.77|P-value:4.09E-4 ID:SGTC_1379|Compound:2144-0755|FD-Score:4.02|P-value:1.81E-4 ID:SGTC_1648|Compound:st011736|FD-Score:-3.70|P-value:5.00E-4 ID:SGTC_1782|Compound:st039423|FD-Score:-4.34|P-value:5.74E-5 ID:SGTC_1787|Compound:fluconazole|FD-Score:4.14|P-value:1.20E-4 ID:SGTC_1790|Compound:st045711|FD-Score:-3.62|P-value:6.41E-4 ID:SGTC_2107|Compound:5363541|FD-Score:4.85|P-value:8.18E-6 ID:SGTC_2305|Compound:7443522|FD-Score:5.52|P-value:4.71E-7 ID:SGTC_2399|Compound:5884133|FD-Score:3.97|P-value:2.10E-4 ID:SGTC_2415|Compound:st077333|FD-Score:-4.66|P-value:1.70E-5 ID:SGTC_2514|Compound:salinomycin|FD-Score:4.49|P-value:3.30E-5 ID:SGTC_2575|Compound:plumbagin|FD-Score:3.91|P-value:2.62E-4 ID:SGTC_2790|Compound:5423970|FD-Score:4.33|P-value:6.10E-5 ID:SGTC_2884|Compound:9058502|FD-Score:-3.85|P-value:3.13E-4 ID:SGTC_3021|Compound:9087658|FD-Score:4.43|P-value:4.23E-5 ID:SGTC_3071|Compound:9117417|FD-Score:4.33|P-value:5.97E-5 ID:SGTC_3100|Compound:9120536|FD-Score:5.13|P-value:2.59E-6 ID:SGTC_3138|Compound:9097216|FD-Score:-3.98|P-value:2.03E-4 ID:SGTC_3142|Compound:9086498|FD-Score:3.66|P-value:5.73E-4 ID:SGTC_3269|Compound:9137823|FD-Score:3.92|P-value:2.47E-4

Top fitness defect scores for YNL324W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_58 0957-0393 14.40 9.0 µM 1.26E-37 cell wall 29 3.68E-47
2 SGTC_865 0488-0499 13.70 301.0 µM 1.43E-34 1 2.72E-43
3 SGTC_553 k007-0256 7.71 45.7 µM 3.71E-12 heme biosynthesis & mitochondrial translocase 4 6.12E-15
4 SGTC_1308 1187-1617 5.99 41.4 µM 5.25E-8 fatty acid desaturase (OLE1) 9 1.06E-9
5 SGTC_172 1112-0046 5.58 2.6 µM 3.67E-7 NEO1 15 1.22E-8
6 SGTC_2305 7443522 5.52 69.2 µM 4.71E-7 1 1.67E-8
7 SGTC_310 1273-0058 5.47 1.1 µM 6.01E-7 mitochondrial stress 165 2.27E-8
8 SGTC_1343 1487-0095 5.27 224.0 µM 1.43E-6 1 6.73E-8
9 SGTC_3100 9120536 5.13 49.5 µM 2.59E-6 4 1.43E-7
10 SGTC_2107 5363541 4.85 170.2 µM 8.18E-6 2 6.06E-7
11 SGTC_887 0885-0007 4.61 9.6 µM 2.16E-5 endomembrane recycling 26 2.05E-6
12 SGTC_313 3474-0040 4.50 5.0 µM 3.25E-5 NEO1-PIK1 51 3.43E-6
13 SGTC_2514 salinomycin 4.49 42.7 µM 3.30E-5 31 3.49E-6
14 SGTC_3021 9087658 4.43 49.5 µM 4.23E-5 5 4.76E-6
15 SGTC_3071 9117417 4.33 49.5 µM 5.97E-5 fatty acid desaturase (OLE1) 13 7.33E-6
16 SGTC_2790 5423970 4.33 71.4 µM 6.10E-5 endomembrane recycling 55 7.54E-6
17 SGTC_1787 fluconazole 4.14 80.0 µM 1.20E-4 iron homeostasis 22 1.76E-5
18 SGTC_555 4296-0685 4.09 37.0 µM 1.42E-4 ergosterol biosynthesis 6 2.17E-5
19 SGTC_208 k072-0098 4.08 12.4 µM 1.45E-4 heme biosynthesis & mitochondrial translocase 40 2.23E-5
20 SGTC_1379 2144-0755 4.02 36.5 µM 1.81E-4 20 2.95E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.245 7.10E-47 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.241 1.15E-45 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.220 4.32E-38 YBR260C RGD1 hom GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
0.206 1.41E-33 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.198 4.70E-31 YGR217W CCH1 hom Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together
0.198 5.74E-31 YKL115C_d YKL115C_d hom Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1
0.192 3.63E-29 YIL160C POT1 hom 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
0.188 4.81E-28 YNL325C FIG4 hom Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain
0.185 2.75E-27 YAL015C NTG1 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
0.185 3.85E-27 YBR120C CBP6 hom Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.180 6.97E-26 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.178 1.94E-25 YNL211C_p YNL211C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene
0.176 6.84E-25 YNL314W DAL82 hom Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain
0.175 1.29E-24 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.175 1.52E-24 YNL291C MID1 hom N-glycosylated integral membrane protein of the ER membrane and plasma membrane, functions as a stretch-activated Ca2+-permeable cation channel required for Ca2+ influx stimulated by pheromone; interacts with Cch1p; forms an oligomer