YNL326C / PFA3

Palmitoyltransferase for Vac8p, required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions

Zygosity: Homozygous strain
fixedexpanded
Profile for YNL326C / PFA3

Click on Significant Values for Screen Details ID:SGTC_3|Compound:0833-0587|FD-Score:3.27|P-value:6.79E-4 ID:SGTC_7|Compound:0192-1582|FD-Score:3.59|P-value:2.12E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.33|P-value:4.28E-4 ID:SGTC_185|Compound:k048-0037|FD-Score:-3.72|P-value:1.00E-4 ID:SGTC_197|Compound:4544-0043|FD-Score:3.17|P-value:9.70E-4 ID:SGTC_211|Compound:3455-0679|FD-Score:-3.83|P-value:6.38E-5 ID:SGTC_470|Compound:aa-861|FD-Score:3.81|P-value:9.30E-5 ID:SGTC_553|Compound:k007-0256|FD-Score:3.26|P-value:7.09E-4 ID:SGTC_911|Compound:3456-4043|FD-Score:4.82|P-value:1.06E-6 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:3.62|P-value:1.95E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:3.42|P-value:4.09E-4 ID:SGTC_1272|Compound:0839-0021|FD-Score:-5.01|P-value:3.00E-7 ID:SGTC_1277|Compound:0873-0036|FD-Score:3.71|P-value:1.37E-4 ID:SGTC_1285|Compound:0929-0069|FD-Score:4.27|P-value:1.38E-5 ID:SGTC_1539|Compound:benzyladenosine|FD-Score:3.97|P-value:4.90E-5 ID:SGTC_1727|Compound:st034309|FD-Score:3.79|P-value:1.01E-4 ID:SGTC_1731|Compound:st035964|FD-Score:3.33|P-value:5.51E-4 ID:SGTC_1807|Compound:st050092|FD-Score:4.29|P-value:1.27E-5 ID:SGTC_2005|Compound:4024620|FD-Score:3.43|P-value:3.91E-4 ID:SGTC_2017|Compound:5132927|FD-Score:3.57|P-value:2.37E-4 ID:SGTC_2018|Compound:5133068|FD-Score:3.89|P-value:6.80E-5 ID:SGTC_2154|Compound:5570129|FD-Score:4.31|P-value:1.18E-5 ID:SGTC_2159|Compound:5658611|FD-Score:5.60|P-value:1.76E-8 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.99|P-value:4.59E-5 ID:SGTC_2467|Compound:5474540|FD-Score:3.75|P-value:1.18E-4 ID:SGTC_2501|Compound:sodium tetradecyl sulfate|FD-Score:-3.34|P-value:4.11E-4 ID:SGTC_2543|Compound:pimpinellin|FD-Score:-3.09|P-value:9.95E-4 ID:SGTC_2643|Compound:anethole|FD-Score:3.51|P-value:2.92E-4 ID:SGTC_2864|Compound:9034755|FD-Score:3.32|P-value:5.66E-4 ID:SGTC_2865|Compound:9030313|FD-Score:4.05|P-value:3.54E-5 ID:SGTC_3000|Compound:9075062|FD-Score:3.21|P-value:8.31E-4 ID:SGTC_3088|Compound:9117578|FD-Score:3.79|P-value:1.00E-4 ID:SGTC_3098|Compound:9119039|FD-Score:5.75|P-value:7.52E-9 ID:SGTC_3223|Compound:9129970|FD-Score:4.21|P-value:1.79E-5 ID:SGTC_3227|Compound:9130768|FD-Score:3.83|P-value:8.51E-5 ID:SGTC_3|Compound:0833-0587|FD-Score:3.27|P-value:6.79E-4 ID:SGTC_7|Compound:0192-1582|FD-Score:3.59|P-value:2.12E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.33|P-value:4.28E-4 ID:SGTC_185|Compound:k048-0037|FD-Score:-3.72|P-value:1.00E-4 ID:SGTC_197|Compound:4544-0043|FD-Score:3.17|P-value:9.70E-4 ID:SGTC_211|Compound:3455-0679|FD-Score:-3.83|P-value:6.38E-5 ID:SGTC_470|Compound:aa-861|FD-Score:3.81|P-value:9.30E-5 ID:SGTC_553|Compound:k007-0256|FD-Score:3.26|P-value:7.09E-4 ID:SGTC_911|Compound:3456-4043|FD-Score:4.82|P-value:1.06E-6 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:3.62|P-value:1.95E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:3.42|P-value:4.09E-4 ID:SGTC_1272|Compound:0839-0021|FD-Score:-5.01|P-value:3.00E-7 ID:SGTC_1277|Compound:0873-0036|FD-Score:3.71|P-value:1.37E-4 ID:SGTC_1285|Compound:0929-0069|FD-Score:4.27|P-value:1.38E-5 ID:SGTC_1539|Compound:benzyladenosine|FD-Score:3.97|P-value:4.90E-5 ID:SGTC_1727|Compound:st034309|FD-Score:3.79|P-value:1.01E-4 ID:SGTC_1731|Compound:st035964|FD-Score:3.33|P-value:5.51E-4 ID:SGTC_1807|Compound:st050092|FD-Score:4.29|P-value:1.27E-5 ID:SGTC_2005|Compound:4024620|FD-Score:3.43|P-value:3.91E-4 ID:SGTC_2017|Compound:5132927|FD-Score:3.57|P-value:2.37E-4 ID:SGTC_2018|Compound:5133068|FD-Score:3.89|P-value:6.80E-5 ID:SGTC_2154|Compound:5570129|FD-Score:4.31|P-value:1.18E-5 ID:SGTC_2159|Compound:5658611|FD-Score:5.60|P-value:1.76E-8 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.99|P-value:4.59E-5 ID:SGTC_2467|Compound:5474540|FD-Score:3.75|P-value:1.18E-4 ID:SGTC_2501|Compound:sodium tetradecyl sulfate|FD-Score:-3.34|P-value:4.11E-4 ID:SGTC_2543|Compound:pimpinellin|FD-Score:-3.09|P-value:9.95E-4 ID:SGTC_2643|Compound:anethole|FD-Score:3.51|P-value:2.92E-4 ID:SGTC_2864|Compound:9034755|FD-Score:3.32|P-value:5.66E-4 ID:SGTC_2865|Compound:9030313|FD-Score:4.05|P-value:3.54E-5 ID:SGTC_3000|Compound:9075062|FD-Score:3.21|P-value:8.31E-4 ID:SGTC_3088|Compound:9117578|FD-Score:3.79|P-value:1.00E-4 ID:SGTC_3098|Compound:9119039|FD-Score:5.75|P-value:7.52E-9 ID:SGTC_3223|Compound:9129970|FD-Score:4.21|P-value:1.79E-5 ID:SGTC_3227|Compound:9130768|FD-Score:3.83|P-value:8.51E-5

Top fitness defect scores for YNL326C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_3098 9119039 5.75 49.5 µM 7.52E-9 Golgi 7 4.39E-9
2 SGTC_2159 5658611 5.60 73.8 µM 1.76E-8 1 1.05E-8
3 SGTC_911 3456-4043 4.82 236.0 µM 1.06E-6 mitochondrial processes 14 7.00E-7
4 SGTC_2154 5570129 4.31 200.0 µM 1.18E-5 9 8.32E-6
5 SGTC_1807 st050092 4.29 58.9 µM 1.27E-5 60S ribosome export 5 8.97E-6
6 SGTC_1285 0929-0069 4.27 65.5 µM 1.38E-5 7 9.78E-6
7 SGTC_3223 9129970 4.21 49.5 µM 1.79E-5 19 1.28E-5
8 SGTC_2865 9030313 4.05 52.0 µM 3.54E-5 11 2.58E-5
9 SGTC_2249 haloperidol 3.99 116.4 µM 4.59E-5 ergosterol depletion effects on membrane 110 3.37E-5
10 SGTC_1539 benzyladenosine 3.97 56.0 µM 4.90E-5 15 3.60E-5
11 SGTC_2018 5133068 3.89 143.0 µM 6.80E-5 24 5.04E-5
12 SGTC_3227 9130768 3.83 49.5 µM 8.51E-5 8 6.35E-5
13 SGTC_470 aa-861 3.81 153.0 µM 9.30E-5 sphingolipid biosynthesis & PDR1 61 6.95E-5
14 SGTC_3088 9117578 3.79 49.5 µM 1.00E-4 amide catabolism 10 7.51E-5
15 SGTC_1727 st034309 3.79 9.9 µM 1.01E-4 49 7.57E-5
16 SGTC_2467 5474540 3.75 200.0 µM 1.18E-4 21 8.89E-5
17 SGTC_1277 0873-0036 3.71 22.0 µM 1.37E-4 PDR1 32 1.03E-4
18 SGTC_915 methyl methanesulfonate (MMS) 3.62 110.0 mM 1.95E-4 DNA damage response 56 1.49E-4
19 SGTC_7 0192-1582 3.59 4.2 µM 2.12E-4 78 1.62E-4
20 SGTC_2017 5132927 3.57 60.6 µM 2.37E-4 40 1.81E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.097 1.78E-8 YDR034C LYS14 hom Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer
0.093 6.98E-8 YOL090W MSH2 hom Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP
0.092 9.96E-8 YDR266C HEL2 hom RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; computational analysis suggests a role as a transcription factor
0.088 2.97E-7 YDL053C PBP4 hom Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress
0.083 1.55E-6 YGL087C MMS2 hom Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress
0.081 2.28E-6 YDL138W RGT2 hom Plasma membrane high glucose sensor that regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; RGT2 has a paralog, SNF3, that arose from the whole genome duplication
0.080 3.15E-6 YOL151W GRE2 hom 3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase (D-lactaldehyde dehydrogenase); stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress
0.080 3.32E-6 YPR171W BSP1 hom Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton
0.079 4.93E-6 YIL109C SEC24 het Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p
0.078 6.12E-6 YIL039W TED1 hom Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
0.075 1.25E-5 YHR156C LIN1 hom Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication
0.075 1.30E-5 YOR030W DFG16 hom Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p
0.075 1.41E-5 YER052C HOM3 hom Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
0.075 1.48E-5 YNR004W_p SWM2_p hom Putative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds
0.075 1.49E-5 YMR059W SEN15 het Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p