YNR001C / CIT1

Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein; CIT1 has a paralog, CIT2, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YNR001C / CIT1

Click on Significant Values for Screen Details ID:SGTC_3|Compound:0833-0587|FD-Score:3.37|P-value:5.67E-4 ID:SGTC_34|Compound:0469-0683|FD-Score:3.37|P-value:5.50E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:4.11|P-value:3.49E-5 ID:SGTC_160|Compound:3453-0671|FD-Score:-3.54|P-value:2.70E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:3.68|P-value:1.85E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:4.30|P-value:1.58E-5 ID:SGTC_213|Compound:1246-0773|FD-Score:3.46|P-value:4.14E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:-3.57|P-value:2.42E-4 ID:SGTC_366|Compound:0181-0206|FD-Score:3.67|P-value:1.92E-4 ID:SGTC_369|Compound:0957-0393|FD-Score:4.45|P-value:8.03E-6 ID:SGTC_381|Compound:3461-2219|FD-Score:4.13|P-value:3.23E-5 ID:SGTC_385|Compound:1494-0393|FD-Score:4.01|P-value:5.17E-5 ID:SGTC_469|Compound:w7|FD-Score:4.74|P-value:2.16E-6 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:-3.46|P-value:3.61E-4 ID:SGTC_488|Compound:nitrendipine|FD-Score:4.08|P-value:3.94E-5 ID:SGTC_519|Compound:k048-0011|FD-Score:-3.44|P-value:3.80E-4 ID:SGTC_523|Compound:1934-0222|FD-Score:3.74|P-value:1.50E-4 ID:SGTC_598|Compound:3937-0236|FD-Score:3.77|P-value:1.33E-4 ID:SGTC_683|Compound:panaxydol|FD-Score:4.26|P-value:1.83E-5 ID:SGTC_691|Compound:1548-0686|FD-Score:3.79|P-value:1.22E-4 ID:SGTC_710|Compound:3448-4187|FD-Score:3.34|P-value:6.16E-4 ID:SGTC_712|Compound:r015-0009|FD-Score:5.80|P-value:9.30E-9 ID:SGTC_736|Compound:0958-0254|FD-Score:5.25|P-value:1.77E-7 ID:SGTC_944|Compound:3370-0224|FD-Score:3.41|P-value:4.86E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:4.18|P-value:2.62E-5 ID:SGTC_1125|Compound:tnp00248|FD-Score:-3.54|P-value:2.69E-4 ID:SGTC_1149|Compound:3909-7961|FD-Score:3.68|P-value:1.83E-4 ID:SGTC_1345|Compound:1488-0192|FD-Score:3.34|P-value:6.21E-4 ID:SGTC_1505|Compound:k297-0037|FD-Score:3.79|P-value:1.22E-4 ID:SGTC_1539|Compound:benzyladenosine|FD-Score:-3.48|P-value:3.30E-4 ID:SGTC_1579|Compound:chlormadinone acetate|FD-Score:3.28|P-value:7.57E-4 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:-3.45|P-value:3.76E-4 ID:SGTC_1755|Compound:st041960|FD-Score:4.08|P-value:3.98E-5 ID:SGTC_1765|Compound:st044815|FD-Score:3.27|P-value:7.81E-4 ID:SGTC_1920|Compound:st075728|FD-Score:-3.28|P-value:6.60E-4 ID:SGTC_1925|Compound:st057770|FD-Score:5.14|P-value:3.13E-7 ID:SGTC_2058|Compound:5236698|FD-Score:-3.53|P-value:2.80E-4 ID:SGTC_2064|Compound:5220758|FD-Score:3.65|P-value:2.09E-4 ID:SGTC_2179|Compound:6298986|FD-Score:6.47|P-value:1.80E-10 ID:SGTC_2181|Compound:6316240|FD-Score:4.31|P-value:1.51E-5 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:-4.19|P-value:2.10E-5 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:3.35|P-value:5.92E-4 ID:SGTC_2646|Compound:retinol|FD-Score:5.71|P-value:1.52E-8 ID:SGTC_2752|Compound:diclazuril|FD-Score:8.54|P-value:5.62E-17 ID:SGTC_2754|Compound:granisetron|FD-Score:3.22|P-value:9.28E-4 ID:SGTC_2837|Compound:9008476|FD-Score:8.75|P-value:1.00E-17 ID:SGTC_2848|Compound:9016370|FD-Score:3.60|P-value:2.44E-4 ID:SGTC_2858|Compound:9025781|FD-Score:7.60|P-value:8.36E-14 ID:SGTC_2932|Compound:9016711|FD-Score:6.58|P-value:8.77E-11 ID:SGTC_2953|Compound:9074162|FD-Score:4.12|P-value:3.29E-5 ID:SGTC_2983|Compound:9022671|FD-Score:3.34|P-value:6.18E-4 ID:SGTC_3065|Compound:9113888|FD-Score:4.75|P-value:2.08E-6 ID:SGTC_3153|Compound:9100258|FD-Score:-3.94|P-value:5.86E-5 ID:SGTC_3260|Compound:9137109|FD-Score:3.78|P-value:1.29E-4 ID:SGTC_3299|Compound:9121982|FD-Score:3.69|P-value:1.81E-4 ID:SGTC_3|Compound:0833-0587|FD-Score:3.37|P-value:5.67E-4 ID:SGTC_34|Compound:0469-0683|FD-Score:3.37|P-value:5.50E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:4.11|P-value:3.49E-5 ID:SGTC_160|Compound:3453-0671|FD-Score:-3.54|P-value:2.70E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:3.68|P-value:1.85E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:4.30|P-value:1.58E-5 ID:SGTC_213|Compound:1246-0773|FD-Score:3.46|P-value:4.14E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:-3.57|P-value:2.42E-4 ID:SGTC_366|Compound:0181-0206|FD-Score:3.67|P-value:1.92E-4 ID:SGTC_369|Compound:0957-0393|FD-Score:4.45|P-value:8.03E-6 ID:SGTC_381|Compound:3461-2219|FD-Score:4.13|P-value:3.23E-5 ID:SGTC_385|Compound:1494-0393|FD-Score:4.01|P-value:5.17E-5 ID:SGTC_469|Compound:w7|FD-Score:4.74|P-value:2.16E-6 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:-3.46|P-value:3.61E-4 ID:SGTC_488|Compound:nitrendipine|FD-Score:4.08|P-value:3.94E-5 ID:SGTC_519|Compound:k048-0011|FD-Score:-3.44|P-value:3.80E-4 ID:SGTC_523|Compound:1934-0222|FD-Score:3.74|P-value:1.50E-4 ID:SGTC_598|Compound:3937-0236|FD-Score:3.77|P-value:1.33E-4 ID:SGTC_683|Compound:panaxydol|FD-Score:4.26|P-value:1.83E-5 ID:SGTC_691|Compound:1548-0686|FD-Score:3.79|P-value:1.22E-4 ID:SGTC_710|Compound:3448-4187|FD-Score:3.34|P-value:6.16E-4 ID:SGTC_712|Compound:r015-0009|FD-Score:5.80|P-value:9.30E-9 ID:SGTC_736|Compound:0958-0254|FD-Score:5.25|P-value:1.77E-7 ID:SGTC_944|Compound:3370-0224|FD-Score:3.41|P-value:4.86E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:4.18|P-value:2.62E-5 ID:SGTC_1125|Compound:tnp00248|FD-Score:-3.54|P-value:2.69E-4 ID:SGTC_1149|Compound:3909-7961|FD-Score:3.68|P-value:1.83E-4 ID:SGTC_1345|Compound:1488-0192|FD-Score:3.34|P-value:6.21E-4 ID:SGTC_1505|Compound:k297-0037|FD-Score:3.79|P-value:1.22E-4 ID:SGTC_1539|Compound:benzyladenosine|FD-Score:-3.48|P-value:3.30E-4 ID:SGTC_1579|Compound:chlormadinone acetate|FD-Score:3.28|P-value:7.57E-4 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:-3.45|P-value:3.76E-4 ID:SGTC_1755|Compound:st041960|FD-Score:4.08|P-value:3.98E-5 ID:SGTC_1765|Compound:st044815|FD-Score:3.27|P-value:7.81E-4 ID:SGTC_1920|Compound:st075728|FD-Score:-3.28|P-value:6.60E-4 ID:SGTC_1925|Compound:st057770|FD-Score:5.14|P-value:3.13E-7 ID:SGTC_2058|Compound:5236698|FD-Score:-3.53|P-value:2.80E-4 ID:SGTC_2064|Compound:5220758|FD-Score:3.65|P-value:2.09E-4 ID:SGTC_2179|Compound:6298986|FD-Score:6.47|P-value:1.80E-10 ID:SGTC_2181|Compound:6316240|FD-Score:4.31|P-value:1.51E-5 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:-4.19|P-value:2.10E-5 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:3.35|P-value:5.92E-4 ID:SGTC_2646|Compound:retinol|FD-Score:5.71|P-value:1.52E-8 ID:SGTC_2752|Compound:diclazuril|FD-Score:8.54|P-value:5.62E-17 ID:SGTC_2754|Compound:granisetron|FD-Score:3.22|P-value:9.28E-4 ID:SGTC_2837|Compound:9008476|FD-Score:8.75|P-value:1.00E-17 ID:SGTC_2848|Compound:9016370|FD-Score:3.60|P-value:2.44E-4 ID:SGTC_2858|Compound:9025781|FD-Score:7.60|P-value:8.36E-14 ID:SGTC_2932|Compound:9016711|FD-Score:6.58|P-value:8.77E-11 ID:SGTC_2953|Compound:9074162|FD-Score:4.12|P-value:3.29E-5 ID:SGTC_2983|Compound:9022671|FD-Score:3.34|P-value:6.18E-4 ID:SGTC_3065|Compound:9113888|FD-Score:4.75|P-value:2.08E-6 ID:SGTC_3153|Compound:9100258|FD-Score:-3.94|P-value:5.86E-5 ID:SGTC_3260|Compound:9137109|FD-Score:3.78|P-value:1.29E-4 ID:SGTC_3299|Compound:9121982|FD-Score:3.69|P-value:1.81E-4

Top fitness defect scores for YNR001C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2837 9008476 8.75 45.5 µM 1.00E-17 1 1.05E-18
2 SGTC_2752 diclazuril 8.54 33.1 µM 5.62E-17 29 6.48E-18
3 SGTC_2858 9025781 7.60 9.0 µM 8.36E-14 sphingolipid biosynthesis & PDR1 32 1.48E-14
4 SGTC_2932 9016711 6.58 44.5 µM 8.77E-11 11 2.34E-11
5 SGTC_2179 6298986 6.47 200.0 µM 1.80E-10 heme requiring 1 5.02E-11
6 SGTC_712 r015-0009 5.80 80.6 µM 9.30E-9 10 3.27E-9
7 SGTC_2646 retinol 5.71 96.8 µM 1.52E-8 1 5.52E-9
8 SGTC_736 0958-0254 5.25 92.6 µM 1.77E-7 Golgi 8 7.41E-8
9 SGTC_1925 st057770 5.14 10.1 µM 3.13E-7 TSC3-RPN4 5 1.36E-7
10 SGTC_3065 9113888 4.75 49.5 µM 2.08E-6 12 1.01E-6
11 SGTC_469 w7 4.74 172.0 µM 2.16E-6 ERG2 4 1.05E-6
12 SGTC_369 0957-0393 4.45 4.5 µM 8.03E-6 cell wall signaling 13 4.21E-6
13 SGTC_2181 6316240 4.31 54.3 µM 1.51E-5 15 8.24E-6
14 SGTC_178 4092-0806 4.30 87.5 µM 1.58E-5 ergosterol depletion effects on membrane 79 8.62E-6
15 SGTC_683 panaxydol 4.26 45.0 µM 1.83E-5 sphingolipid biosynthesis & PDR1 29 1.01E-5
16 SGTC_1119 parthenolide 4.18 318.5 nM 2.62E-5 TSC3-RPN4 30 1.47E-5
17 SGTC_381 3461-2219 4.13 42.7 µM 3.23E-5 copper-dependent oxidative stress 16 1.84E-5
18 SGTC_2953 9074162 4.12 66.5 µM 3.29E-5 18 1.88E-5
19 SGTC_157 1348-1695 4.11 65.8 µM 3.49E-5 iron homeostasis 62 2.00E-5
20 SGTC_488 nitrendipine 4.08 139.0 µM 3.94E-5 46 2.27E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.196 2.64E-30 YDR392W SPT3 hom Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
0.149 3.13E-18 YIL170W HXT12 hom Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family
0.148 8.46E-18 YLR055C SPT8 hom Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters
0.135 4.34E-15 YGL066W SGF73 hom SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay
0.132 1.33E-14 YHR112C YHR112C hom Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway
0.129 5.85E-14 YPL254W HFI1 hom Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions
0.125 4.31E-13 YBL079W NUP170 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication
0.113 6.07E-11 YDR120C TRM1 hom tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments
0.107 6.03E-10 YLR060W FRS1 het Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control
0.106 6.22E-10 YEL060C PRB1 hom Vacuolar proteinase B (yscB); serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress
0.105 1.26E-9 YJR033C RAV1 hom Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate
0.104 1.47E-9 YOL050C_d YOL050C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
0.104 1.71E-9 YPL055C LGE1 hom Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division
0.102 3.06E-9 YDR073W SNF11 hom Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions
0.101 4.78E-9 YLR087C CSF1 hom Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies