YNR003C / RPC34

RNA polymerase III subunit C34; interacts with TFIIIB70 and is a key determinant in pol III recruitment by the preinitiation complex

Zygosity: Heterozygous strain
fixedexpanded
Profile for YNR003C / RPC34

Click on Significant Values for Screen Details ID:SGTC_23|Compound:0213-0011|FD-Score:-2.88|P-value:6.48E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:5.12|P-value:5.73E-9 ID:SGTC_115|Compound:0527-0192|FD-Score:2.92|P-value:5.78E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:6.91|P-value:6.53E-15 ID:SGTC_203|Compound:k066-0004|FD-Score:3.14|P-value:2.36E-4 ID:SGTC_211|Compound:3455-0679|FD-Score:-3.43|P-value:6.35E-5 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.18|P-value:1.96E-4 ID:SGTC_262|Compound:fenpropimorph|FD-Score:4.76|P-value:5.64E-8 ID:SGTC_324|Compound:1315-0088|FD-Score:3.58|P-value:3.27E-5 ID:SGTC_341|Compound:1486-1430|FD-Score:9.11|P-value:1.45E-24 ID:SGTC_351|Compound:caspofungin|FD-Score:2.83|P-value:8.15E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:5.84|P-value:3.83E-11 ID:SGTC_360|Compound:0957-0399|FD-Score:3.03|P-value:3.66E-4 ID:SGTC_441|Compound:propafenone|FD-Score:3.99|P-value:4.41E-6 ID:SGTC_573|Compound:3702-0553|FD-Score:-3.54|P-value:3.76E-5 ID:SGTC_586|Compound:0957-0265|FD-Score:8.51|P-value:1.16E-21 ID:SGTC_634|Compound:1611-4317|FD-Score:4.66|P-value:9.95E-8 ID:SGTC_788|Compound:1182-0090|FD-Score:3.99|P-value:4.30E-6 ID:SGTC_829|Compound:1635-0120|FD-Score:3.31|P-value:1.13E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:4.82|P-value:3.95E-8 ID:SGTC_916|Compound:4466-0038|FD-Score:2.99|P-value:4.36E-4 ID:SGTC_1009|Compound:4106-0057|FD-Score:4.80|P-value:4.27E-8 ID:SGTC_1133|Compound:3473-0247|FD-Score:4.75|P-value:6.07E-8 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.94|P-value:5.68E-6 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:3.98|P-value:4.54E-6 ID:SGTC_1650|Compound:st011916|FD-Score:3.34|P-value:1.00E-4 ID:SGTC_1658|Compound:st012944|FD-Score:2.86|P-value:7.17E-4 ID:SGTC_1739|Compound:st037196|FD-Score:3.33|P-value:1.04E-4 ID:SGTC_1794|Compound:st048918|FD-Score:2.90|P-value:6.07E-4 ID:SGTC_2058|Compound:5236698|FD-Score:-3.49|P-value:4.73E-5 ID:SGTC_2059|Compound:5238311|FD-Score:-3.69|P-value:1.90E-5 ID:SGTC_2241|Compound:6772625|FD-Score:4.82|P-value:3.89E-8 ID:SGTC_2396|Compound:5342950|FD-Score:2.82|P-value:8.37E-4 ID:SGTC_2403|Compound:6032726|FD-Score:-2.87|P-value:6.66E-4 ID:SGTC_2421|Compound:5690543|FD-Score:3.19|P-value:1.88E-4 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:-2.85|P-value:7.19E-4 ID:SGTC_2516|Compound:gossypol|FD-Score:-2.95|P-value:4.83E-4 ID:SGTC_2522|Compound:biochanin a|FD-Score:2.85|P-value:7.46E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:3.07|P-value:3.17E-4 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:2.95|P-value:5.12E-4 ID:SGTC_2630|Compound:antimycin a|FD-Score:-4.18|P-value:1.54E-6 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:9.58|P-value:5.68E-27 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:-3.71|P-value:1.70E-5 ID:SGTC_2709|Compound:st078560|FD-Score:-3.65|P-value:2.26E-5 ID:SGTC_2932|Compound:9016711|FD-Score:5.09|P-value:6.72E-9 ID:SGTC_2933|Compound:9026794|FD-Score:3.99|P-value:4.26E-6 ID:SGTC_3026|Compound:9090633|FD-Score:4.11|P-value:2.32E-6 ID:SGTC_3341|Compound:9147222|FD-Score:4.31|P-value:7.78E-7 ID:SGTC_23|Compound:0213-0011|FD-Score:-2.88|P-value:6.48E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:5.12|P-value:5.73E-9 ID:SGTC_115|Compound:0527-0192|FD-Score:2.92|P-value:5.78E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:6.91|P-value:6.53E-15 ID:SGTC_203|Compound:k066-0004|FD-Score:3.14|P-value:2.36E-4 ID:SGTC_211|Compound:3455-0679|FD-Score:-3.43|P-value:6.35E-5 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.18|P-value:1.96E-4 ID:SGTC_262|Compound:fenpropimorph|FD-Score:4.76|P-value:5.64E-8 ID:SGTC_324|Compound:1315-0088|FD-Score:3.58|P-value:3.27E-5 ID:SGTC_341|Compound:1486-1430|FD-Score:9.11|P-value:1.45E-24 ID:SGTC_351|Compound:caspofungin|FD-Score:2.83|P-value:8.15E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:5.84|P-value:3.83E-11 ID:SGTC_360|Compound:0957-0399|FD-Score:3.03|P-value:3.66E-4 ID:SGTC_441|Compound:propafenone|FD-Score:3.99|P-value:4.41E-6 ID:SGTC_573|Compound:3702-0553|FD-Score:-3.54|P-value:3.76E-5 ID:SGTC_586|Compound:0957-0265|FD-Score:8.51|P-value:1.16E-21 ID:SGTC_634|Compound:1611-4317|FD-Score:4.66|P-value:9.95E-8 ID:SGTC_788|Compound:1182-0090|FD-Score:3.99|P-value:4.30E-6 ID:SGTC_829|Compound:1635-0120|FD-Score:3.31|P-value:1.13E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:4.82|P-value:3.95E-8 ID:SGTC_916|Compound:4466-0038|FD-Score:2.99|P-value:4.36E-4 ID:SGTC_1009|Compound:4106-0057|FD-Score:4.80|P-value:4.27E-8 ID:SGTC_1133|Compound:3473-0247|FD-Score:4.75|P-value:6.07E-8 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.94|P-value:5.68E-6 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:3.98|P-value:4.54E-6 ID:SGTC_1650|Compound:st011916|FD-Score:3.34|P-value:1.00E-4 ID:SGTC_1658|Compound:st012944|FD-Score:2.86|P-value:7.17E-4 ID:SGTC_1739|Compound:st037196|FD-Score:3.33|P-value:1.04E-4 ID:SGTC_1794|Compound:st048918|FD-Score:2.90|P-value:6.07E-4 ID:SGTC_2058|Compound:5236698|FD-Score:-3.49|P-value:4.73E-5 ID:SGTC_2059|Compound:5238311|FD-Score:-3.69|P-value:1.90E-5 ID:SGTC_2241|Compound:6772625|FD-Score:4.82|P-value:3.89E-8 ID:SGTC_2396|Compound:5342950|FD-Score:2.82|P-value:8.37E-4 ID:SGTC_2403|Compound:6032726|FD-Score:-2.87|P-value:6.66E-4 ID:SGTC_2421|Compound:5690543|FD-Score:3.19|P-value:1.88E-4 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:-2.85|P-value:7.19E-4 ID:SGTC_2516|Compound:gossypol|FD-Score:-2.95|P-value:4.83E-4 ID:SGTC_2522|Compound:biochanin a|FD-Score:2.85|P-value:7.46E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:3.07|P-value:3.17E-4 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:2.95|P-value:5.12E-4 ID:SGTC_2630|Compound:antimycin a|FD-Score:-4.18|P-value:1.54E-6 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:9.58|P-value:5.68E-27 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:-3.71|P-value:1.70E-5 ID:SGTC_2709|Compound:st078560|FD-Score:-3.65|P-value:2.26E-5 ID:SGTC_2932|Compound:9016711|FD-Score:5.09|P-value:6.72E-9 ID:SGTC_2933|Compound:9026794|FD-Score:3.99|P-value:4.26E-6 ID:SGTC_3026|Compound:9090633|FD-Score:4.11|P-value:2.32E-6 ID:SGTC_3341|Compound:9147222|FD-Score:4.31|P-value:7.78E-7

Top fitness defect scores for YNR003C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2631 bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone 9.58 100.0 µM 5.68E-27 1 4.73E-22 4.27
2 SGTC_341 1486-1430 9.11 230.0 µM 1.45E-24 2 4.08E-20 0.20
3 SGTC_586 0957-0265 8.51 8.2 µM 1.16E-21 cell wall 12 8.91E-18 0.01
4 SGTC_138 0850-0259 6.91 40.9 µM 6.53E-15 cell wall 23 2.45E-12 0.08
5 SGTC_354 2783-3500 5.84 4.9 µM 3.83E-11 cell wall 14 2.69E-9 0.00
6 SGTC_58 0957-0393 5.12 9.0 µM 5.73E-9 cell wall 37 1.54E-7 0.04
7 SGTC_2932 9016711 5.09 44.5 µM 6.72E-9 4 1.75E-7 0.40
8 SGTC_2241 6772625 4.82 37.8 µM 3.89E-8 4 7.24E-7 0.18
9 SGTC_833 3852-0053 4.82 558.0 µM 3.95E-8 3 7.34E-7 0.91
10 SGTC_1009 4106-0057 4.80 9.9 µM 4.27E-8 5 7.81E-7 0.41
11 SGTC_262 fenpropimorph 4.76 62.5 µM 5.64E-8 ergosterol depletion effects on membrane 12 9.78E-7 0.37
12 SGTC_1133 3473-0247 4.75 50.0 µM 6.07E-8 RSC complex & mRNA processing 35 1.04E-6 0.07
13 SGTC_634 1611-4317 4.66 11.2 µM 9.95E-8 ERG2 5 1.55E-6 0.46
14 SGTC_3341 9147222 4.31 25.9 µM 7.78E-7 RNA processing & uracil transport 7 8.19E-6 0.26
15 SGTC_3026 9090633 4.11 49.5 µM 2.32E-6 5 1.99E-5 0.11
16 SGTC_2933 9026794 3.99 32.5 µM 4.26E-6 4 3.25E-5 0.15
17 SGTC_788 1182-0090 3.99 188.0 µM 4.30E-6 endomembrane recycling 13 3.28E-5 0.51
18 SGTC_441 propafenone 3.99 146.0 µM 4.41E-6 4 3.34E-5 0.46
19 SGTC_1565 sinapyl alcohol 3.98 95.1 µM 4.54E-6 2 3.43E-5 0.14
20 SGTC_1528 arabinosylhypoxanthine 3.94 74.6 µM 5.68E-6 4 4.11E-5 0.27

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.276 8.12E-60 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.226 4.45E-40 YNL233W BNI4 hom Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p
0.224 1.41E-39 YBR091C TIM12 het Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins
0.224 1.61E-39 YDR351W SBE2 hom Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth
0.209 1.64E-34 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.204 5.76E-33 YLR150W STM1 hom Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
0.204 8.52E-33 YLL043W FPS1 hom Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress
0.197 1.47E-30 YBR143C SUP45 het Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p
0.188 3.49E-28 YOR127W RGA1 hom GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.188 5.02E-28 YLR332W MID2 hom O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p
0.183 1.09E-26 YAL015C NTG1 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
0.181 3.20E-26 YJL099W CHS6 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.180 5.92E-26 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.180 9.73E-26 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.180 9.83E-26 YNL045W LAP2 hom Leucyl aminopeptidase yscIV (leukotriene A4 hydrolase) with epoxide hydrolase activity, metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus