YNR006W / VPS27

Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p)

Zygosity: Homozygous strain
fixedexpanded
Profile for YNR006W / VPS27

Click on Significant Values for Screen Details ID:SGTC_37|Compound:0566-0070|FD-Score:4.89|P-value:2.48E-5 ID:SGTC_40|Compound:0327-0103|FD-Score:-5.98|P-value:3.41E-7 ID:SGTC_58|Compound:0957-0393|FD-Score:-5.40|P-value:3.58E-6 ID:SGTC_70|Compound:0141-0289|FD-Score:4.12|P-value:3.11E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:6.66|P-value:1.65E-8 ID:SGTC_150|Compound:3970-0969|FD-Score:6.89|P-value:5.30E-9 ID:SGTC_159|Compound:3970-0959|FD-Score:13.30|P-value:1.39E-28 ID:SGTC_168|Compound:3970-0862|FD-Score:5.91|P-value:4.73E-7 ID:SGTC_214|Compound:4373-3496|FD-Score:7.37|P-value:4.85E-10 ID:SGTC_263|Compound:alizarin|FD-Score:5.05|P-value:1.41E-5 ID:SGTC_274|Compound:sirtinol|FD-Score:-4.73|P-value:4.32E-5 ID:SGTC_365|Compound:0121-0052|FD-Score:4.08|P-value:3.52E-4 ID:SGTC_376|Compound:3970-0862|FD-Score:4.39|P-value:1.34E-4 ID:SGTC_377|Compound:3970-0964|FD-Score:5.50|P-value:2.49E-6 ID:SGTC_404|Compound:fumagillin|FD-Score:3.87|P-value:6.59E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-6.60|P-value:2.12E-8 ID:SGTC_470|Compound:aa-861|FD-Score:4.23|P-value:2.23E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:5.72|P-value:1.01E-6 ID:SGTC_525|Compound:k781-2675|FD-Score:5.88|P-value:5.18E-7 ID:SGTC_568|Compound:1193-0097|FD-Score:5.49|P-value:2.56E-6 ID:SGTC_712|Compound:r015-0009|FD-Score:4.68|P-value:5.19E-5 ID:SGTC_748|Compound:0136-0228|FD-Score:6.13|P-value:1.81E-7 ID:SGTC_751|Compound:0833-0568|FD-Score:3.73|P-value:9.87E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:-3.99|P-value:4.59E-4 ID:SGTC_952|Compound:1185-0122|FD-Score:-5.18|P-value:8.45E-6 ID:SGTC_965|Compound:1291-5240|FD-Score:6.04|P-value:2.71E-7 ID:SGTC_1239|Compound:0337-0584|FD-Score:4.51|P-value:9.04E-5 ID:SGTC_1250|Compound:0485-0349|FD-Score:-3.76|P-value:8.87E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:-4.90|P-value:2.35E-5 ID:SGTC_1437|Compound:4092-1038|FD-Score:4.41|P-value:1.26E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:10.50|P-value:1.42E-18 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:7.78|P-value:5.39E-11 ID:SGTC_1648|Compound:st011736|FD-Score:-3.73|P-value:9.82E-4 ID:SGTC_1658|Compound:st012944|FD-Score:4.52|P-value:8.90E-5 ID:SGTC_1687|Compound:st020778|FD-Score:10.00|P-value:5.17E-17 ID:SGTC_1713|Compound:st033146|FD-Score:-3.73|P-value:9.60E-4 ID:SGTC_1773|Compound:st048542|FD-Score:5.11|P-value:1.10E-5 ID:SGTC_1788|Compound:fluconazole|FD-Score:5.22|P-value:7.44E-6 ID:SGTC_1844|Compound:rapamycin|FD-Score:14.40|P-value:4.28E-33 ID:SGTC_1853|Compound:st056710|FD-Score:4.44|P-value:1.15E-4 ID:SGTC_1909|Compound:st060837|FD-Score:-6.01|P-value:2.94E-7 ID:SGTC_1910|Compound:st062352|FD-Score:-3.79|P-value:8.25E-4 ID:SGTC_1911|Compound:st060208|FD-Score:4.95|P-value:2.01E-5 ID:SGTC_1917|Compound:st066507|FD-Score:-4.37|P-value:1.42E-4 ID:SGTC_1938|Compound:st074717|FD-Score:4.49|P-value:9.81E-5 ID:SGTC_1949|Compound:st076024|FD-Score:-4.35|P-value:1.52E-4 ID:SGTC_2067|Compound:5224629|FD-Score:4.69|P-value:4.92E-5 ID:SGTC_2077|Compound:5212015|FD-Score:5.54|P-value:2.10E-6 ID:SGTC_2090|Compound:5301565|FD-Score:4.19|P-value:2.51E-4 ID:SGTC_2110|Compound:5483698|FD-Score:4.13|P-value:3.04E-4 ID:SGTC_2114|Compound:5491910|FD-Score:3.89|P-value:6.29E-4 ID:SGTC_2163|Compound:5671233|FD-Score:-4.64|P-value:5.87E-5 ID:SGTC_2170|Compound:morpholine|FD-Score:4.04|P-value:4.00E-4 ID:SGTC_2172|Compound:5754775|FD-Score:3.96|P-value:5.07E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-4.68|P-value:5.06E-5 ID:SGTC_2283|Compound:7944793|FD-Score:3.88|P-value:6.42E-4 ID:SGTC_2360|Compound:9040895|FD-Score:6.59|P-value:2.28E-8 ID:SGTC_2406|Compound:ethylparaben|FD-Score:5.95|P-value:3.93E-7 ID:SGTC_2484|Compound:5809818|FD-Score:-3.82|P-value:7.57E-4 ID:SGTC_2486|Compound:5283202|FD-Score:3.87|P-value:6.54E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:5.55|P-value:2.05E-6 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:4.99|P-value:1.69E-5 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:5.67|P-value:1.28E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.45|P-value:3.03E-6 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:8.14|P-value:7.01E-12 ID:SGTC_2619|Compound:obliquin|FD-Score:3.90|P-value:6.02E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:6.98|P-value:3.38E-9 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.99|P-value:4.63E-4 ID:SGTC_2698|Compound:st077697|FD-Score:3.86|P-value:6.80E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:8.17|P-value:5.82E-12 ID:SGTC_2841|Compound:9004761|FD-Score:4.36|P-value:1.46E-4 ID:SGTC_2852|Compound:9023854|FD-Score:4.52|P-value:8.85E-5 ID:SGTC_2854|Compound:9024559|FD-Score:4.29|P-value:1.84E-4 ID:SGTC_2862|Compound:9027634|FD-Score:6.12|P-value:1.87E-7 ID:SGTC_2878|Compound:9043973|FD-Score:3.81|P-value:7.78E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-3.74|P-value:9.46E-4 ID:SGTC_2940|Compound:9060638|FD-Score:-3.91|P-value:5.73E-4 ID:SGTC_3091|Compound:9116372|FD-Score:3.72|P-value:9.96E-4 ID:SGTC_3113|Compound:9123498|FD-Score:3.90|P-value:6.02E-4 ID:SGTC_3123|Compound:9126162|FD-Score:4.68|P-value:5.16E-5 ID:SGTC_3143|Compound:9086658|FD-Score:-6.63|P-value:1.86E-8 ID:SGTC_3145|Compound:9085516|FD-Score:4.79|P-value:3.50E-5 ID:SGTC_3219|Compound:9131112|FD-Score:-3.94|P-value:5.29E-4 ID:SGTC_37|Compound:0566-0070|FD-Score:4.89|P-value:2.48E-5 ID:SGTC_40|Compound:0327-0103|FD-Score:-5.98|P-value:3.41E-7 ID:SGTC_58|Compound:0957-0393|FD-Score:-5.40|P-value:3.58E-6 ID:SGTC_70|Compound:0141-0289|FD-Score:4.12|P-value:3.11E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:6.66|P-value:1.65E-8 ID:SGTC_150|Compound:3970-0969|FD-Score:6.89|P-value:5.30E-9 ID:SGTC_159|Compound:3970-0959|FD-Score:13.30|P-value:1.39E-28 ID:SGTC_168|Compound:3970-0862|FD-Score:5.91|P-value:4.73E-7 ID:SGTC_214|Compound:4373-3496|FD-Score:7.37|P-value:4.85E-10 ID:SGTC_263|Compound:alizarin|FD-Score:5.05|P-value:1.41E-5 ID:SGTC_274|Compound:sirtinol|FD-Score:-4.73|P-value:4.32E-5 ID:SGTC_365|Compound:0121-0052|FD-Score:4.08|P-value:3.52E-4 ID:SGTC_376|Compound:3970-0862|FD-Score:4.39|P-value:1.34E-4 ID:SGTC_377|Compound:3970-0964|FD-Score:5.50|P-value:2.49E-6 ID:SGTC_404|Compound:fumagillin|FD-Score:3.87|P-value:6.59E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-6.60|P-value:2.12E-8 ID:SGTC_470|Compound:aa-861|FD-Score:4.23|P-value:2.23E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:5.72|P-value:1.01E-6 ID:SGTC_525|Compound:k781-2675|FD-Score:5.88|P-value:5.18E-7 ID:SGTC_568|Compound:1193-0097|FD-Score:5.49|P-value:2.56E-6 ID:SGTC_712|Compound:r015-0009|FD-Score:4.68|P-value:5.19E-5 ID:SGTC_748|Compound:0136-0228|FD-Score:6.13|P-value:1.81E-7 ID:SGTC_751|Compound:0833-0568|FD-Score:3.73|P-value:9.87E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:-3.99|P-value:4.59E-4 ID:SGTC_952|Compound:1185-0122|FD-Score:-5.18|P-value:8.45E-6 ID:SGTC_965|Compound:1291-5240|FD-Score:6.04|P-value:2.71E-7 ID:SGTC_1239|Compound:0337-0584|FD-Score:4.51|P-value:9.04E-5 ID:SGTC_1250|Compound:0485-0349|FD-Score:-3.76|P-value:8.87E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:-4.90|P-value:2.35E-5 ID:SGTC_1437|Compound:4092-1038|FD-Score:4.41|P-value:1.26E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:10.50|P-value:1.42E-18 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:7.78|P-value:5.39E-11 ID:SGTC_1648|Compound:st011736|FD-Score:-3.73|P-value:9.82E-4 ID:SGTC_1658|Compound:st012944|FD-Score:4.52|P-value:8.90E-5 ID:SGTC_1687|Compound:st020778|FD-Score:10.00|P-value:5.17E-17 ID:SGTC_1713|Compound:st033146|FD-Score:-3.73|P-value:9.60E-4 ID:SGTC_1773|Compound:st048542|FD-Score:5.11|P-value:1.10E-5 ID:SGTC_1788|Compound:fluconazole|FD-Score:5.22|P-value:7.44E-6 ID:SGTC_1844|Compound:rapamycin|FD-Score:14.40|P-value:4.28E-33 ID:SGTC_1853|Compound:st056710|FD-Score:4.44|P-value:1.15E-4 ID:SGTC_1909|Compound:st060837|FD-Score:-6.01|P-value:2.94E-7 ID:SGTC_1910|Compound:st062352|FD-Score:-3.79|P-value:8.25E-4 ID:SGTC_1911|Compound:st060208|FD-Score:4.95|P-value:2.01E-5 ID:SGTC_1917|Compound:st066507|FD-Score:-4.37|P-value:1.42E-4 ID:SGTC_1938|Compound:st074717|FD-Score:4.49|P-value:9.81E-5 ID:SGTC_1949|Compound:st076024|FD-Score:-4.35|P-value:1.52E-4 ID:SGTC_2067|Compound:5224629|FD-Score:4.69|P-value:4.92E-5 ID:SGTC_2077|Compound:5212015|FD-Score:5.54|P-value:2.10E-6 ID:SGTC_2090|Compound:5301565|FD-Score:4.19|P-value:2.51E-4 ID:SGTC_2110|Compound:5483698|FD-Score:4.13|P-value:3.04E-4 ID:SGTC_2114|Compound:5491910|FD-Score:3.89|P-value:6.29E-4 ID:SGTC_2163|Compound:5671233|FD-Score:-4.64|P-value:5.87E-5 ID:SGTC_2170|Compound:morpholine|FD-Score:4.04|P-value:4.00E-4 ID:SGTC_2172|Compound:5754775|FD-Score:3.96|P-value:5.07E-4 ID:SGTC_2250|Compound:alverine citrate|FD-Score:-4.68|P-value:5.06E-5 ID:SGTC_2283|Compound:7944793|FD-Score:3.88|P-value:6.42E-4 ID:SGTC_2360|Compound:9040895|FD-Score:6.59|P-value:2.28E-8 ID:SGTC_2406|Compound:ethylparaben|FD-Score:5.95|P-value:3.93E-7 ID:SGTC_2484|Compound:5809818|FD-Score:-3.82|P-value:7.57E-4 ID:SGTC_2486|Compound:5283202|FD-Score:3.87|P-value:6.54E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:5.55|P-value:2.05E-6 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:4.99|P-value:1.69E-5 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:5.67|P-value:1.28E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.45|P-value:3.03E-6 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:8.14|P-value:7.01E-12 ID:SGTC_2619|Compound:obliquin|FD-Score:3.90|P-value:6.02E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:6.98|P-value:3.38E-9 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.99|P-value:4.63E-4 ID:SGTC_2698|Compound:st077697|FD-Score:3.86|P-value:6.80E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:8.17|P-value:5.82E-12 ID:SGTC_2841|Compound:9004761|FD-Score:4.36|P-value:1.46E-4 ID:SGTC_2852|Compound:9023854|FD-Score:4.52|P-value:8.85E-5 ID:SGTC_2854|Compound:9024559|FD-Score:4.29|P-value:1.84E-4 ID:SGTC_2862|Compound:9027634|FD-Score:6.12|P-value:1.87E-7 ID:SGTC_2878|Compound:9043973|FD-Score:3.81|P-value:7.78E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-3.74|P-value:9.46E-4 ID:SGTC_2940|Compound:9060638|FD-Score:-3.91|P-value:5.73E-4 ID:SGTC_3091|Compound:9116372|FD-Score:3.72|P-value:9.96E-4 ID:SGTC_3113|Compound:9123498|FD-Score:3.90|P-value:6.02E-4 ID:SGTC_3123|Compound:9126162|FD-Score:4.68|P-value:5.16E-5 ID:SGTC_3143|Compound:9086658|FD-Score:-6.63|P-value:1.86E-8 ID:SGTC_3145|Compound:9085516|FD-Score:4.79|P-value:3.50E-5 ID:SGTC_3219|Compound:9131112|FD-Score:-3.94|P-value:5.29E-4

Top fitness defect scores for YNR006W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1844 rapamycin 14.40 4.0 nM 4.28E-33 16 4.30E-47
2 SGTC_159 3970-0959 13.30 50.0 µM 1.39E-28 2 1.44E-40
3 SGTC_1495 4469-0029 10.50 237.0 µM 1.42E-18 14 4.31E-26
4 SGTC_1687 st020778 10.00 55.4 µM 5.17E-17 49 7.75E-24
5 SGTC_2737 bleomycin 8.17 30.5 nM 5.82E-12 Golgi 20 1.51E-16
6 SGTC_2615 purpurogallin-4-carboxylic acid 8.14 100.0 µM 7.01E-12 redox potentiating 17 1.98E-16
7 SGTC_1593 justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one 7.78 54.9 µM 5.39E-11 redox potentiating 20 3.74E-15
8 SGTC_214 4373-3496 7.37 70.0 µM 4.85E-10 9 8.84E-14
9 SGTC_2679 rapamycin 6.98 1.0 nM 3.38E-9 60 1.45E-12
10 SGTC_150 3970-0969 6.89 77.2 µM 5.30E-9 15 2.77E-12
11 SGTC_149 3970-0822 6.66 46.1 µM 1.65E-8 tubulin folding & SWR complex 26 1.41E-11
12 SGTC_2360 9040895 6.59 200.0 µM 2.28E-8 RPP1 & pyrimidine depletion 2 2.26E-11
13 SGTC_748 0136-0228 6.13 77.9 µM 1.81E-7 redox potentiating 33 4.42E-10
14 SGTC_2862 9027634 6.12 71.4 µM 1.87E-7 ERG2 10 4.61E-10
15 SGTC_965 1291-5240 6.04 308.0 µM 2.71E-7 5 7.88E-10
16 SGTC_2406 ethylparaben 5.95 405.7 µM 3.93E-7 9 1.34E-9
17 SGTC_168 3970-0862 5.91 530.5 µM 4.73E-7 46 1.75E-9
18 SGTC_525 k781-2675 5.88 26.7 µM 5.18E-7 redox potentiating 27 2.00E-9
19 SGTC_524 r073-0010 5.72 27.8 µM 1.01E-6 18 5.22E-9
20 SGTC_2567 aureobasidin a 5.67 60.0 nM 1.28E-6 45 7.28E-9

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.350 4.17E-97 YPR173C VPS4 hom AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism
0.349 9.41E-97 YLR119W SRN2 hom Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus
0.347 1.22E-95 YDR323C PEP7 hom Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
0.339 9.67E-91 YKL041W VPS24 hom One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway
0.319 8.66E-80 YKL002W DID4 hom Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis
0.310 2.10E-75 YOR036W PEP12 hom Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
0.301 4.78E-71 YPL084W BRO1 hom Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes
0.293 2.64E-67 YDR495C VPS3 hom Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
0.292 7.41E-67 YGL212W VAM7 hom Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
0.255 5.52E-51 YLR025W SNF7 hom One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes
0.252 1.27E-49 YDR080W VPS41 hom Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport
0.245 7.49E-47 YDL077C VAM6 hom Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p
0.244 1.27E-46 YOR089C VPS21 hom Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
0.238 2.44E-44 YPL065W VPS28 hom Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p
0.237 6.82E-44 YLR417W VPS36 hom Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome