Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase
Zygosity: Homozygous strain
fixedexpanded
![]() ![]() Click on Significant Values for Screen Details |
Top fitness defect scores for YNR025C deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Correlation | pval | ORF | Gene | Zygosity | Description |
---|---|---|---|---|---|
0.153 | 6.19E-19 | YDR056C_p | YDR056C_p | hom | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein |
0.129 | 5.20E-14 | YKL202W_d | YKL202W_d | hom | Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data |
0.126 | 2.13E-13 | YKL004W | AUR1 | het | Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance |
0.109 | 2.28E-10 | YDR510W | SMT3 | het | Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 |
0.105 | 9.51E-10 | YNL134C | YNL134C | hom | Protein of unknown function; similar to dehydrogenases from other model organisms; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress |
0.096 | 2.44E-8 | YDR026C | NSI1 | hom | RNA polymerase I termination factor; binds to rDNA terminator element, required for efficient Pol I termination; required for rDNA silencing at NTS1; facilities association of Sir2p with NTS1, contributes to rDNA stability and cell longevity; interacts physically with Fob1p and RENT subunits, Sir2p and Net1p; may interact with ribosomes, based on co-purification experiments; Myb-like DNA-binding protein; NSI1 has a paralog, REB1, that arose from the whole genome duplication |
0.096 | 2.79E-8 | YNL140C_p | YNL140C_p | hom | Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C |
0.091 | 1.34E-7 | YDR308C | SRB7 | het | Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p |
0.090 | 1.88E-7 | YER064C | VHR2 | hom | Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress |
0.089 | 2.57E-7 | YBR277C_d | YBR277C_d | hom | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W |
0.087 | 4.54E-7 | YIL146C | ATG32 | hom | Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane |
0.086 | 6.83E-7 | YBL004W | UTP20 | het | Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA |
0.081 | 2.89E-6 | YNR028W | CPR8 | hom | Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway |
0.080 | 3.33E-6 | YDR094W_d | YDR094W_d | hom | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2 |
0.080 | 3.52E-6 | YDL167C | NRP1 | hom | Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis |