YNR026C / SEC12

Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER

Zygosity: Heterozygous strain
fixedexpanded
Profile for YNR026C / SEC12

Click on Significant Values for Screen Details ID:SGTC_4|Compound:0960-0234|FD-Score:-4.93|P-value:8.00E-8 ID:SGTC_16|Compound:erodoxin|FD-Score:3.05|P-value:5.86E-4 ID:SGTC_20|Compound:0481-0013|FD-Score:6.45|P-value:3.56E-12 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:-2.98|P-value:7.75E-4 ID:SGTC_89|Compound:0302-0250|FD-Score:3.28|P-value:2.46E-4 ID:SGTC_142|Compound:0335-0849|FD-Score:3.04|P-value:6.26E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:3.80|P-value:2.73E-5 ID:SGTC_450|Compound:aminopyrene|FD-Score:3.38|P-value:1.65E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.32|P-value:2.05E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:3.30|P-value:2.30E-4 ID:SGTC_575|Compound:k072-0246|FD-Score:3.90|P-value:1.71E-5 ID:SGTC_577|Compound:r071-0003|FD-Score:3.42|P-value:1.36E-4 ID:SGTC_586|Compound:0957-0265|FD-Score:-2.99|P-value:7.32E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:3.78|P-value:2.88E-5 ID:SGTC_801|Compound:0866-0028|FD-Score:7.13|P-value:1.71E-14 ID:SGTC_899|Compound:1083-0020|FD-Score:2.98|P-value:7.79E-4 ID:SGTC_1053|Compound:3366-3204|FD-Score:5.91|P-value:1.61E-10 ID:SGTC_1061|Compound:3448-9325|FD-Score:3.62|P-value:5.86E-5 ID:SGTC_1071|Compound:trifluoperazine|FD-Score:3.02|P-value:6.59E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.81|P-value:2.57E-5 ID:SGTC_1152|Compound:3909-8331|FD-Score:3.37|P-value:1.70E-4 ID:SGTC_1159|Compound:4092-0538|FD-Score:2.91|P-value:9.93E-4 ID:SGTC_1244|Compound:0416-0015|FD-Score:3.10|P-value:4.83E-4 ID:SGTC_1389|Compound:3405-0187|FD-Score:-2.91|P-value:9.81E-4 ID:SGTC_1396|Compound:3511-0007|FD-Score:-3.24|P-value:2.87E-4 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:-3.22|P-value:3.05E-4 ID:SGTC_1853|Compound:st056710|FD-Score:-2.99|P-value:7.31E-4 ID:SGTC_1935|Compound:st074532|FD-Score:3.52|P-value:9.14E-5 ID:SGTC_1989|Compound:st071868|FD-Score:4.37|P-value:1.70E-6 ID:SGTC_2138|Compound:5575593|FD-Score:-3.63|P-value:5.72E-5 ID:SGTC_2148|Compound:5546263|FD-Score:3.23|P-value:2.98E-4 ID:SGTC_2153|Compound:5569715|FD-Score:3.00|P-value:7.20E-4 ID:SGTC_2156|Compound:5649887|FD-Score:3.58|P-value:7.08E-5 ID:SGTC_2201|Compound:6605497|FD-Score:3.80|P-value:2.67E-5 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:3.29|P-value:2.35E-4 ID:SGTC_2239|Compound:6685329|FD-Score:2.95|P-value:8.54E-4 ID:SGTC_2267|Compound:7931562|FD-Score:4.26|P-value:2.92E-6 ID:SGTC_2282|Compound:7944268|FD-Score:-3.03|P-value:6.27E-4 ID:SGTC_2301|Compound:7769476|FD-Score:3.01|P-value:6.86E-4 ID:SGTC_2358|Compound:9037157|FD-Score:5.17|P-value:1.97E-8 ID:SGTC_2640|Compound:pentamidine|FD-Score:4.31|P-value:2.31E-6 ID:SGTC_2729|Compound:fluphenazine|FD-Score:3.49|P-value:1.05E-4 ID:SGTC_2783|Compound:7738165|FD-Score:-3.01|P-value:6.94E-4 ID:SGTC_2789|Compound:5281112|FD-Score:-3.76|P-value:3.21E-5 ID:SGTC_2792|Compound:7535235|FD-Score:-3.02|P-value:6.49E-4 ID:SGTC_2830|Compound:7992007|FD-Score:-4.03|P-value:8.98E-6 ID:SGTC_2831|Compound:7999036|FD-Score:4.09|P-value:6.99E-6 ID:SGTC_2834|Compound:9001755|FD-Score:3.69|P-value:4.44E-5 ID:SGTC_2835|Compound:9006588|FD-Score:-2.94|P-value:8.66E-4 ID:SGTC_2881|Compound:9058953|FD-Score:3.62|P-value:6.06E-5 ID:SGTC_3128|Compound:9127691|FD-Score:3.40|P-value:1.48E-4 ID:SGTC_3132|Compound:9126706|FD-Score:5.04|P-value:4.18E-8 ID:SGTC_4|Compound:0960-0234|FD-Score:-4.93|P-value:8.00E-8 ID:SGTC_16|Compound:erodoxin|FD-Score:3.05|P-value:5.86E-4 ID:SGTC_20|Compound:0481-0013|FD-Score:6.45|P-value:3.56E-12 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:-2.98|P-value:7.75E-4 ID:SGTC_89|Compound:0302-0250|FD-Score:3.28|P-value:2.46E-4 ID:SGTC_142|Compound:0335-0849|FD-Score:3.04|P-value:6.26E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:3.80|P-value:2.73E-5 ID:SGTC_450|Compound:aminopyrene|FD-Score:3.38|P-value:1.65E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.32|P-value:2.05E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:3.30|P-value:2.30E-4 ID:SGTC_575|Compound:k072-0246|FD-Score:3.90|P-value:1.71E-5 ID:SGTC_577|Compound:r071-0003|FD-Score:3.42|P-value:1.36E-4 ID:SGTC_586|Compound:0957-0265|FD-Score:-2.99|P-value:7.32E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:3.78|P-value:2.88E-5 ID:SGTC_801|Compound:0866-0028|FD-Score:7.13|P-value:1.71E-14 ID:SGTC_899|Compound:1083-0020|FD-Score:2.98|P-value:7.79E-4 ID:SGTC_1053|Compound:3366-3204|FD-Score:5.91|P-value:1.61E-10 ID:SGTC_1061|Compound:3448-9325|FD-Score:3.62|P-value:5.86E-5 ID:SGTC_1071|Compound:trifluoperazine|FD-Score:3.02|P-value:6.59E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.81|P-value:2.57E-5 ID:SGTC_1152|Compound:3909-8331|FD-Score:3.37|P-value:1.70E-4 ID:SGTC_1159|Compound:4092-0538|FD-Score:2.91|P-value:9.93E-4 ID:SGTC_1244|Compound:0416-0015|FD-Score:3.10|P-value:4.83E-4 ID:SGTC_1389|Compound:3405-0187|FD-Score:-2.91|P-value:9.81E-4 ID:SGTC_1396|Compound:3511-0007|FD-Score:-3.24|P-value:2.87E-4 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:-3.22|P-value:3.05E-4 ID:SGTC_1853|Compound:st056710|FD-Score:-2.99|P-value:7.31E-4 ID:SGTC_1935|Compound:st074532|FD-Score:3.52|P-value:9.14E-5 ID:SGTC_1989|Compound:st071868|FD-Score:4.37|P-value:1.70E-6 ID:SGTC_2138|Compound:5575593|FD-Score:-3.63|P-value:5.72E-5 ID:SGTC_2148|Compound:5546263|FD-Score:3.23|P-value:2.98E-4 ID:SGTC_2153|Compound:5569715|FD-Score:3.00|P-value:7.20E-4 ID:SGTC_2156|Compound:5649887|FD-Score:3.58|P-value:7.08E-5 ID:SGTC_2201|Compound:6605497|FD-Score:3.80|P-value:2.67E-5 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:3.29|P-value:2.35E-4 ID:SGTC_2239|Compound:6685329|FD-Score:2.95|P-value:8.54E-4 ID:SGTC_2267|Compound:7931562|FD-Score:4.26|P-value:2.92E-6 ID:SGTC_2282|Compound:7944268|FD-Score:-3.03|P-value:6.27E-4 ID:SGTC_2301|Compound:7769476|FD-Score:3.01|P-value:6.86E-4 ID:SGTC_2358|Compound:9037157|FD-Score:5.17|P-value:1.97E-8 ID:SGTC_2640|Compound:pentamidine|FD-Score:4.31|P-value:2.31E-6 ID:SGTC_2729|Compound:fluphenazine|FD-Score:3.49|P-value:1.05E-4 ID:SGTC_2783|Compound:7738165|FD-Score:-3.01|P-value:6.94E-4 ID:SGTC_2789|Compound:5281112|FD-Score:-3.76|P-value:3.21E-5 ID:SGTC_2792|Compound:7535235|FD-Score:-3.02|P-value:6.49E-4 ID:SGTC_2830|Compound:7992007|FD-Score:-4.03|P-value:8.98E-6 ID:SGTC_2831|Compound:7999036|FD-Score:4.09|P-value:6.99E-6 ID:SGTC_2834|Compound:9001755|FD-Score:3.69|P-value:4.44E-5 ID:SGTC_2835|Compound:9006588|FD-Score:-2.94|P-value:8.66E-4 ID:SGTC_2881|Compound:9058953|FD-Score:3.62|P-value:6.06E-5 ID:SGTC_3128|Compound:9127691|FD-Score:3.40|P-value:1.48E-4 ID:SGTC_3132|Compound:9126706|FD-Score:5.04|P-value:4.18E-8

Top fitness defect scores for YNR026C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_801 0866-0028 7.13 10.1 µM 1.71E-14 1 5.02E-13 0.85
2 SGTC_20 0481-0013 6.45 4.9 µM 3.56E-12 2 5.69E-11 1.21
3 SGTC_1053 3366-3204 5.91 134.0 µM 1.61E-10 cell wall 3 1.67E-9 1.12
4 SGTC_2358 9037157 5.17 4.0 µM 1.97E-8 7 1.19E-7 0.20
5 SGTC_3132 9126706 5.04 49.5 µM 4.18E-8 3 2.32E-7 0.19
6 SGTC_1989 st071868 4.37 50.2 µM 1.70E-6 4 6.23E-6 0.36
7 SGTC_2640 pentamidine 4.31 10.4 µM 2.31E-6 mitochondrial processes 12 8.19E-6 0.10
8 SGTC_2267 7931562 4.26 200.0 µM 2.92E-6 4 1.01E-5 0.32
9 SGTC_2831 7999036 4.09 19.5 µM 6.99E-6 5 2.19E-5 0.33
10 SGTC_575 k072-0246 3.90 11.9 µM 1.71E-5 6 4.86E-5 0.06
11 SGTC_1083 didemethylchlorpromazine sulfoxide 3.81 707.4 nM 2.57E-5 redox potentiating 25 6.98E-5 0.02
12 SGTC_2201 6605497 3.80 169.9 µM 2.67E-5 ergosterol biosynthesis 4 7.23E-5 0.41
13 SGTC_151 3013-0144 3.80 2.3 µM 2.73E-5 iron homeostasis 12 7.37E-5 0.03
14 SGTC_696 1560-0013 3.78 611.0 µM 2.88E-5 10 7.72E-5 0.13
15 SGTC_2834 9001755 3.69 58.4 µM 4.44E-5 5 1.14E-4 0.02
16 SGTC_1061 3448-9325 3.62 110.0 µM 5.86E-5 8 1.45E-4 0.05
17 SGTC_2881 9058953 3.62 52.0 µM 6.06E-5 8 1.50E-4 0.04
18 SGTC_2156 5649887 3.58 115.0 µM 7.08E-5 RPP1 & pyrimidine depletion 20 1.72E-4 0.10
19 SGTC_1935 st074532 3.52 41.6 µM 9.14E-5 9 2.16E-4 0.07
20 SGTC_2729 fluphenazine 3.49 10.7 µM 1.05E-4 10 2.44E-4 0.08

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.217 7.02E-37 YDR032C PST2 hom Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication
0.153 5.61E-19 YBR258C SHG1 hom Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres
0.124 6.05E-13 YPR122W AXL1 hom Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells
0.121 1.83E-12 YPL251W_d YPL251W_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
0.110 1.82E-10 YMR182W-A_p YMR182W-A_p hom Putative protein of unknown function
0.109 2.50E-10 YPL252C YAH1 het Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
0.106 8.05E-10 YOL036W_p YOL036W_p hom Protein of unknown function; potential Cdc28p substrate; YOL036W has a paralog, YIR016W, that arose from the whole genome duplication
0.105 1.12E-9 YHL005C_d YHL005C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YHL004W
0.102 3.88E-9 YOR018W ROD1 hom Membrane protein, binds the ubiquitin ligase Rsp5p via its 2 PY motifs; overexpression confers resistance to the GST substrate o-dinitrobenzene, zinc, and calcium; proposed to regulate the endocytosis of plasma membrane proteins; protein abundance increases in response to DNA replication stress; ROD1 has a paralog, ROG3, that arose from the whole genome duplication
0.101 4.18E-9 YJR083C ACF4 hom Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate
0.099 8.52E-9 YDR431W_d YDR431W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.095 3.35E-8 YDR208W MSS4 het Phosphatidylinositol-4-phosphate 5-kinase, involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation
0.090 1.95E-7 YKL052C ASK1 het Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress
0.090 2.07E-7 YOR009W TIR4 hom Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth; transcription is also induced by cold shock
0.089 2.37E-7 YDR398W UTP5 het Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit