YNR027W / BUD17

Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK)

Zygosity: Homozygous strain
fixedexpanded
Profile for YNR027W / BUD17

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:-4.21|P-value:2.50E-5 ID:SGTC_70|Compound:0141-0289|FD-Score:3.73|P-value:1.74E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:4.68|P-value:3.70E-6 ID:SGTC_89|Compound:0302-0250|FD-Score:3.80|P-value:1.34E-4 ID:SGTC_128|Compound:0906-2981|FD-Score:-3.60|P-value:2.65E-4 ID:SGTC_136|Compound:0097-0015|FD-Score:3.71|P-value:1.92E-4 ID:SGTC_169|Compound:k015-0017|FD-Score:-3.32|P-value:6.86E-4 ID:SGTC_171|Compound:k064-0041|FD-Score:4.54|P-value:6.69E-6 ID:SGTC_550|Compound:4123-0385|FD-Score:-3.66|P-value:2.14E-4 ID:SGTC_561|Compound:1550-0330|FD-Score:-4.18|P-value:2.92E-5 ID:SGTC_594|Compound:1487-1584|FD-Score:4.60|P-value:5.14E-6 ID:SGTC_677|Compound:0929-0063|FD-Score:3.27|P-value:8.77E-4 ID:SGTC_687|Compound:k029-0044|FD-Score:-3.70|P-value:1.81E-4 ID:SGTC_793|Compound:0488-0545|FD-Score:3.88|P-value:9.93E-5 ID:SGTC_794|Compound:k038-0031|FD-Score:-3.28|P-value:7.85E-4 ID:SGTC_810|Compound:3825-7150|FD-Score:-6.71|P-value:5.37E-11 ID:SGTC_811|Compound:0416-0114|FD-Score:-6.66|P-value:7.39E-11 ID:SGTC_1008|Compound:4106-0037|FD-Score:-3.49|P-value:3.89E-4 ID:SGTC_1024|Compound:4507-0395|FD-Score:4.41|P-value:1.19E-5 ID:SGTC_1734|Compound:st037168|FD-Score:3.67|P-value:2.20E-4 ID:SGTC_1971|Compound:st069429|FD-Score:-3.70|P-value:1.84E-4 ID:SGTC_1975|Compound:st070967|FD-Score:-4.88|P-value:1.30E-6 ID:SGTC_2028|Compound:5153890|FD-Score:3.38|P-value:5.99E-4 ID:SGTC_2240|Compound:6690706|FD-Score:4.11|P-value:4.10E-5 ID:SGTC_2423|Compound:st077119|FD-Score:3.49|P-value:4.15E-4 ID:SGTC_2569|Compound:tetrandrine|FD-Score:-4.57|P-value:5.56E-6 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:3.65|P-value:2.32E-4 ID:SGTC_2715|Compound:st079500|FD-Score:3.41|P-value:5.50E-4 ID:SGTC_2716|Compound:st080858|FD-Score:-3.35|P-value:6.27E-4 ID:SGTC_2857|Compound:9024691|FD-Score:3.45|P-value:4.83E-4 ID:SGTC_2966|Compound:9084041|FD-Score:3.32|P-value:7.30E-4 ID:SGTC_3097|Compound:9117246|FD-Score:3.34|P-value:6.93E-4 ID:SGTC_3249|Compound:9135183|FD-Score:3.92|P-value:8.70E-5 ID:SGTC_3284|Compound:9108171|FD-Score:4.42|P-value:1.13E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:-4.21|P-value:2.50E-5 ID:SGTC_70|Compound:0141-0289|FD-Score:3.73|P-value:1.74E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:4.68|P-value:3.70E-6 ID:SGTC_89|Compound:0302-0250|FD-Score:3.80|P-value:1.34E-4 ID:SGTC_128|Compound:0906-2981|FD-Score:-3.60|P-value:2.65E-4 ID:SGTC_136|Compound:0097-0015|FD-Score:3.71|P-value:1.92E-4 ID:SGTC_169|Compound:k015-0017|FD-Score:-3.32|P-value:6.86E-4 ID:SGTC_171|Compound:k064-0041|FD-Score:4.54|P-value:6.69E-6 ID:SGTC_550|Compound:4123-0385|FD-Score:-3.66|P-value:2.14E-4 ID:SGTC_561|Compound:1550-0330|FD-Score:-4.18|P-value:2.92E-5 ID:SGTC_594|Compound:1487-1584|FD-Score:4.60|P-value:5.14E-6 ID:SGTC_677|Compound:0929-0063|FD-Score:3.27|P-value:8.77E-4 ID:SGTC_687|Compound:k029-0044|FD-Score:-3.70|P-value:1.81E-4 ID:SGTC_793|Compound:0488-0545|FD-Score:3.88|P-value:9.93E-5 ID:SGTC_794|Compound:k038-0031|FD-Score:-3.28|P-value:7.85E-4 ID:SGTC_810|Compound:3825-7150|FD-Score:-6.71|P-value:5.37E-11 ID:SGTC_811|Compound:0416-0114|FD-Score:-6.66|P-value:7.39E-11 ID:SGTC_1008|Compound:4106-0037|FD-Score:-3.49|P-value:3.89E-4 ID:SGTC_1024|Compound:4507-0395|FD-Score:4.41|P-value:1.19E-5 ID:SGTC_1734|Compound:st037168|FD-Score:3.67|P-value:2.20E-4 ID:SGTC_1971|Compound:st069429|FD-Score:-3.70|P-value:1.84E-4 ID:SGTC_1975|Compound:st070967|FD-Score:-4.88|P-value:1.30E-6 ID:SGTC_2028|Compound:5153890|FD-Score:3.38|P-value:5.99E-4 ID:SGTC_2240|Compound:6690706|FD-Score:4.11|P-value:4.10E-5 ID:SGTC_2423|Compound:st077119|FD-Score:3.49|P-value:4.15E-4 ID:SGTC_2569|Compound:tetrandrine|FD-Score:-4.57|P-value:5.56E-6 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:3.65|P-value:2.32E-4 ID:SGTC_2715|Compound:st079500|FD-Score:3.41|P-value:5.50E-4 ID:SGTC_2716|Compound:st080858|FD-Score:-3.35|P-value:6.27E-4 ID:SGTC_2857|Compound:9024691|FD-Score:3.45|P-value:4.83E-4 ID:SGTC_2966|Compound:9084041|FD-Score:3.32|P-value:7.30E-4 ID:SGTC_3097|Compound:9117246|FD-Score:3.34|P-value:6.93E-4 ID:SGTC_3249|Compound:9135183|FD-Score:3.92|P-value:8.70E-5 ID:SGTC_3284|Compound:9108171|FD-Score:4.42|P-value:1.13E-5

Top fitness defect scores for YNR027W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_73 0327-0325 4.68 16.2 µM 3.70E-6 DNA damage response 30 1.46E-6
2 SGTC_594 1487-1584 4.60 10.8 µM 5.14E-6 14 2.09E-6
3 SGTC_171 k064-0041 4.54 9.7 µM 6.69E-6 heme requiring 20 2.78E-6
4 SGTC_3284 9108171 4.42 64.9 µM 1.13E-5 19 4.88E-6
5 SGTC_1024 4507-0395 4.41 26.0 µM 1.19E-5 TSC3-RPN4 17 5.17E-6
6 SGTC_2240 6690706 4.11 185.9 µM 4.10E-5 17 1.98E-5
7 SGTC_3249 9135183 3.92 49.5 µM 8.70E-5 60S ribosome export 10 4.46E-5
8 SGTC_793 0488-0545 3.88 106.0 µM 9.93E-5 17 5.15E-5
9 SGTC_89 0302-0250 3.80 142.1 µM 1.34E-4 22 7.10E-5
10 SGTC_70 0141-0289 3.73 43.3 µM 1.74E-4 mitochondrial stress 192 9.44E-5
11 SGTC_136 0097-0015 3.71 404.5 µM 1.92E-4 60S ribosome export 21 1.05E-4
12 SGTC_1734 st037168 3.67 69.4 µM 2.20E-4 35 1.22E-4
13 SGTC_2572 astragaloside iv 3.65 100.0 µM 2.32E-4 39 1.29E-4
14 SGTC_2423 st077119 3.49 87.2 µM 4.15E-4 22 2.41E-4
15 SGTC_2857 9024691 3.45 58.4 µM 4.83E-4 38 2.85E-4
16 SGTC_2715 st079500 3.41 56.4 µM 5.50E-4 18 3.28E-4
17 SGTC_2028 5153890 3.38 36.4 µM 5.99E-4 cell wall signaling 50 3.59E-4
18 SGTC_3097 9117246 3.34 49.5 µM 6.93E-4 30 4.21E-4
19 SGTC_2966 9084041 3.32 48.0 µM 7.30E-4 59 4.45E-4
20 SGTC_677 0929-0063 3.27 136.0 µM 8.77E-4 26 5.42E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.149 3.20E-18 YBR035C PDX3 hom Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism
0.113 4.70E-11 YDR294C DPL1 hom Dihydrosphingosine phosphate lyase, regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate
0.102 3.53E-9 YLR346C_p YLR346C_p hom Putative protein of unknown function found in mitochondria; expression is regulated by transcription factors involved in pleiotropic drug resistance, Pdr1p and Yrr1p; YLR346C is not an essential gene
0.098 1.28E-8 YDL125C HNT1 hom Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress
0.095 3.08E-8 YBR227C MCX1 hom Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins
0.093 7.22E-8 YBR114W RAD16 hom Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex
0.090 1.85E-7 YPR013C_p CMR3_p hom Putative zinc finger protein; YPR013C is not an essential gene
0.084 1.14E-6 YOR109W INP53 hom Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains
0.082 1.96E-6 YDL182W LYS20 hom Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS20 has a paralog, LYS21, that arose from the whole genome duplication
0.080 3.73E-6 YOR101W RAS1 hom GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress
0.078 6.56E-6 YMR303C ADH2 hom Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1
0.077 8.98E-6 YGL263W COS12 hom Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins
0.075 1.25E-5 YER170W ADK2 hom Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background
0.075 1.42E-5 YBR259W YBR259W hom Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress
0.074 1.65E-5 YOR191W ULS1 hom Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress