YNR031C / SSK2

MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YNR031C / SSK2

Click on Significant Values for Screen Details ID:SGTC_29|Compound:0299-0004|FD-Score:-3.03|P-value:9.58E-4 ID:SGTC_81|Compound:0411-0046|FD-Score:3.17|P-value:6.31E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:4.23|P-value:8.39E-6 ID:SGTC_207|Compound:4185-0010|FD-Score:5.42|P-value:1.67E-8 ID:SGTC_251|Compound:melphalan|FD-Score:4.18|P-value:1.06E-5 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.12|P-value:7.62E-4 ID:SGTC_292|Compound:k038-0015|FD-Score:3.79|P-value:5.79E-5 ID:SGTC_422|Compound:cantharidin|FD-Score:3.81|P-value:5.36E-5 ID:SGTC_425|Compound:3825-7017|FD-Score:3.80|P-value:5.50E-5 ID:SGTC_564|Compound:3555-0129|FD-Score:3.28|P-value:4.21E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:13.40|P-value:1.02E-42 ID:SGTC_704|Compound:1310-0270|FD-Score:3.60|P-value:1.25E-4 ID:SGTC_891|Compound:0929-0073|FD-Score:3.61|P-value:1.22E-4 ID:SGTC_972|Compound:1319-0219|FD-Score:4.02|P-value:2.15E-5 ID:SGTC_1061|Compound:3448-9325|FD-Score:4.00|P-value:2.33E-5 ID:SGTC_1076|Compound:itraconazole|FD-Score:3.57|P-value:1.39E-4 ID:SGTC_1288|Compound:0973-0013|FD-Score:5.80|P-value:1.71E-9 ID:SGTC_1505|Compound:k297-0037|FD-Score:8.13|P-value:5.93E-17 ID:SGTC_1512|Compound:0259-0544|FD-Score:3.39|P-value:2.79E-4 ID:SGTC_1514|Compound:1189-1591|FD-Score:-3.60|P-value:1.16E-4 ID:SGTC_1523|Compound:st012844|FD-Score:-3.65|P-value:9.63E-5 ID:SGTC_1524|Compound:corynanthine|FD-Score:-4.90|P-value:2.70E-7 ID:SGTC_1623|Compound:st003709|FD-Score:4.12|P-value:1.40E-5 ID:SGTC_1710|Compound:st031758|FD-Score:5.04|P-value:1.42E-7 ID:SGTC_1716|Compound:st033144|FD-Score:5.01|P-value:1.70E-7 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.25|P-value:4.49E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:3.33|P-value:3.59E-4 ID:SGTC_1871|Compound:st057448|FD-Score:4.32|P-value:5.49E-6 ID:SGTC_1876|Compound:myriocin|FD-Score:3.14|P-value:7.16E-4 ID:SGTC_2083|Compound:5306046|FD-Score:3.48|P-value:2.00E-4 ID:SGTC_2283|Compound:7944793|FD-Score:3.83|P-value:4.93E-5 ID:SGTC_2334|Compound:9017922|FD-Score:3.67|P-value:9.44E-5 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:3.29|P-value:4.16E-4 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:-5.08|P-value:1.07E-7 ID:SGTC_2533|Compound:agelasine|FD-Score:-3.42|P-value:2.32E-4 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:-3.10|P-value:7.60E-4 ID:SGTC_2589|Compound:lobaric acid|FD-Score:3.23|P-value:5.02E-4 ID:SGTC_2665|Compound:lovastatin|FD-Score:3.17|P-value:6.36E-4 ID:SGTC_2680|Compound:butyl paraben|FD-Score:-3.03|P-value:9.68E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:3.93|P-value:3.12E-5 ID:SGTC_2786|Compound:7743617|FD-Score:3.33|P-value:3.60E-4 ID:SGTC_2805|Compound:7950783|FD-Score:3.70|P-value:8.19E-5 ID:SGTC_2810|Compound:7959871|FD-Score:3.91|P-value:3.44E-5 ID:SGTC_2823|Compound:7987624|FD-Score:3.23|P-value:5.03E-4 ID:SGTC_2896|Compound:9052656|FD-Score:3.07|P-value:9.05E-4 ID:SGTC_2905|Compound:7799065|FD-Score:3.37|P-value:3.08E-4 ID:SGTC_2951|Compound:9072950|FD-Score:-3.15|P-value:6.33E-4 ID:SGTC_3040|Compound:9091894|FD-Score:3.51|P-value:1.75E-4 ID:SGTC_3182|Compound:9108849|FD-Score:3.27|P-value:4.47E-4 ID:SGTC_3204|Compound:9114183|FD-Score:3.32|P-value:3.66E-4 ID:SGTC_3321|Compound:9137793|FD-Score:3.49|P-value:1.93E-4 ID:SGTC_29|Compound:0299-0004|FD-Score:-3.03|P-value:9.58E-4 ID:SGTC_81|Compound:0411-0046|FD-Score:3.17|P-value:6.31E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:4.23|P-value:8.39E-6 ID:SGTC_207|Compound:4185-0010|FD-Score:5.42|P-value:1.67E-8 ID:SGTC_251|Compound:melphalan|FD-Score:4.18|P-value:1.06E-5 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.12|P-value:7.62E-4 ID:SGTC_292|Compound:k038-0015|FD-Score:3.79|P-value:5.79E-5 ID:SGTC_422|Compound:cantharidin|FD-Score:3.81|P-value:5.36E-5 ID:SGTC_425|Compound:3825-7017|FD-Score:3.80|P-value:5.50E-5 ID:SGTC_564|Compound:3555-0129|FD-Score:3.28|P-value:4.21E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:13.40|P-value:1.02E-42 ID:SGTC_704|Compound:1310-0270|FD-Score:3.60|P-value:1.25E-4 ID:SGTC_891|Compound:0929-0073|FD-Score:3.61|P-value:1.22E-4 ID:SGTC_972|Compound:1319-0219|FD-Score:4.02|P-value:2.15E-5 ID:SGTC_1061|Compound:3448-9325|FD-Score:4.00|P-value:2.33E-5 ID:SGTC_1076|Compound:itraconazole|FD-Score:3.57|P-value:1.39E-4 ID:SGTC_1288|Compound:0973-0013|FD-Score:5.80|P-value:1.71E-9 ID:SGTC_1505|Compound:k297-0037|FD-Score:8.13|P-value:5.93E-17 ID:SGTC_1512|Compound:0259-0544|FD-Score:3.39|P-value:2.79E-4 ID:SGTC_1514|Compound:1189-1591|FD-Score:-3.60|P-value:1.16E-4 ID:SGTC_1523|Compound:st012844|FD-Score:-3.65|P-value:9.63E-5 ID:SGTC_1524|Compound:corynanthine|FD-Score:-4.90|P-value:2.70E-7 ID:SGTC_1623|Compound:st003709|FD-Score:4.12|P-value:1.40E-5 ID:SGTC_1710|Compound:st031758|FD-Score:5.04|P-value:1.42E-7 ID:SGTC_1716|Compound:st033144|FD-Score:5.01|P-value:1.70E-7 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.25|P-value:4.49E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:3.33|P-value:3.59E-4 ID:SGTC_1871|Compound:st057448|FD-Score:4.32|P-value:5.49E-6 ID:SGTC_1876|Compound:myriocin|FD-Score:3.14|P-value:7.16E-4 ID:SGTC_2083|Compound:5306046|FD-Score:3.48|P-value:2.00E-4 ID:SGTC_2283|Compound:7944793|FD-Score:3.83|P-value:4.93E-5 ID:SGTC_2334|Compound:9017922|FD-Score:3.67|P-value:9.44E-5 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:3.29|P-value:4.16E-4 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:-5.08|P-value:1.07E-7 ID:SGTC_2533|Compound:agelasine|FD-Score:-3.42|P-value:2.32E-4 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:-3.10|P-value:7.60E-4 ID:SGTC_2589|Compound:lobaric acid|FD-Score:3.23|P-value:5.02E-4 ID:SGTC_2665|Compound:lovastatin|FD-Score:3.17|P-value:6.36E-4 ID:SGTC_2680|Compound:butyl paraben|FD-Score:-3.03|P-value:9.68E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:3.93|P-value:3.12E-5 ID:SGTC_2786|Compound:7743617|FD-Score:3.33|P-value:3.60E-4 ID:SGTC_2805|Compound:7950783|FD-Score:3.70|P-value:8.19E-5 ID:SGTC_2810|Compound:7959871|FD-Score:3.91|P-value:3.44E-5 ID:SGTC_2823|Compound:7987624|FD-Score:3.23|P-value:5.03E-4 ID:SGTC_2896|Compound:9052656|FD-Score:3.07|P-value:9.05E-4 ID:SGTC_2905|Compound:7799065|FD-Score:3.37|P-value:3.08E-4 ID:SGTC_2951|Compound:9072950|FD-Score:-3.15|P-value:6.33E-4 ID:SGTC_3040|Compound:9091894|FD-Score:3.51|P-value:1.75E-4 ID:SGTC_3182|Compound:9108849|FD-Score:3.27|P-value:4.47E-4 ID:SGTC_3204|Compound:9114183|FD-Score:3.32|P-value:3.66E-4 ID:SGTC_3321|Compound:9137793|FD-Score:3.49|P-value:1.93E-4

Top fitness defect scores for YNR031C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_690 glyoxal 13.40 20.3 µM 1.02E-42 7 3.36E-41
2 SGTC_1505 k297-0037 8.13 20.6 µM 5.93E-17 1 2.11E-16
3 SGTC_1288 0973-0013 5.80 65.5 µM 1.71E-9 2 3.23E-9
4 SGTC_207 4185-0010 5.42 4.5 µM 1.67E-8 endomembrane recycling 50 2.91E-8
5 SGTC_1710 st031758 5.04 61.5 µM 1.42E-7 6 2.30E-7
6 SGTC_1716 st033144 5.01 30.6 µM 1.70E-7 6 2.73E-7
7 SGTC_1871 st057448 4.32 67.3 µM 5.49E-6 17 7.78E-6
8 SGTC_168 3970-0862 4.23 530.5 µM 8.39E-6 102 1.17E-5
9 SGTC_251 melphalan 4.18 456.5 µM 1.06E-5 DNA damage response 16 1.46E-5
10 SGTC_1623 st003709 4.12 20.3 µM 1.40E-5 calcium & mitochondrial duress 62 1.92E-5
11 SGTC_972 1319-0219 4.02 195.0 µM 2.15E-5 26 2.91E-5
12 SGTC_1061 3448-9325 4.00 110.0 µM 2.33E-5 24 3.14E-5
13 SGTC_2756 carbadox 3.93 75.1 µM 3.12E-5 59 4.16E-5
14 SGTC_2810 7959871 3.91 45.5 µM 3.44E-5 copper-dependent oxidative stress 42 4.57E-5
15 SGTC_2283 7944793 3.83 200.0 µM 4.93E-5 52 6.47E-5
16 SGTC_422 cantharidin 3.81 155.0 µM 5.36E-5 cell wall signaling 61 7.02E-5
17 SGTC_425 3825-7017 3.80 13.1 µM 5.50E-5 26 7.20E-5
18 SGTC_292 k038-0015 3.79 474.0 µM 5.79E-5 7 7.56E-5
19 SGTC_2805 7950783 3.70 58.4 µM 8.19E-5 40 1.06E-4
20 SGTC_2334 9017922 3.67 200.0 µM 9.44E-5 31 1.21E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.265 3.62E-55 YGR190C_d YGR190C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W
0.244 1.11E-46 YGR077C PEX8 hom Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
0.221 1.85E-38 YLR113W HOG1 hom Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress
0.196 2.18E-30 YOR364W_d YOR364W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C
0.193 1.78E-29 YKL104C GFA1 het Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication
0.192 2.35E-29 YNL307C MCK1 hom Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family
0.178 2.90E-25 YKL213C DOA1 hom WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress
0.169 7.60E-23 YML113W DAT1 hom DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; not essential for viability
0.167 2.28E-22 YLR070C XYL2 hom Xylitol dehydrogenase, converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect
0.167 2.57E-22 YBL024W NCL1 hom S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120
0.154 2.60E-19 YJL110C GZF3 hom GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication
0.139 5.38E-16 YMR275C BUL1 hom Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication
0.137 1.62E-15 YGL133W ITC1 hom Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex
0.136 2.12E-15 YMR260C TIF11 het Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3
0.135 4.27E-15 YJL128C PBS2 hom MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition