YNR032C-A / HUB1

Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear

Zygosity: Homozygous strain
fixedexpanded
Profile for YNR032C-A / HUB1

Click on Significant Values for Screen Details ID:SGTC_176|Compound:1486-1328|FD-Score:4.74|P-value:1.22E-7 ID:SGTC_180|Compound:1486-1293|FD-Score:4.00|P-value:6.63E-6 ID:SGTC_182|Compound:3448-4371|FD-Score:4.80|P-value:8.30E-8 ID:SGTC_209|Compound:4077-0173|FD-Score:3.03|P-value:4.76E-4 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:2.88|P-value:8.41E-4 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.71|P-value:2.61E-5 ID:SGTC_848|Compound:0368-0089|FD-Score:3.07|P-value:4.02E-4 ID:SGTC_862|Compound:0452-0056|FD-Score:2.86|P-value:8.83E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:3.66|P-value:3.33E-5 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:5.54|P-value:8.28E-10 ID:SGTC_1098|Compound:clofazimine|FD-Score:3.43|P-value:9.11E-5 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:4.28|P-value:1.53E-6 ID:SGTC_1204|Compound:4049-0315|FD-Score:-4.73|P-value:1.66E-7 ID:SGTC_1246|Compound:0422-0085|FD-Score:3.13|P-value:3.13E-4 ID:SGTC_1256|Compound:0570-0116|FD-Score:-3.06|P-value:4.93E-4 ID:SGTC_1365|Compound:1672-3266|FD-Score:3.57|P-value:4.96E-5 ID:SGTC_1404|Compound:3909-7820|FD-Score:3.25|P-value:1.93E-4 ID:SGTC_1491|Compound:4138-0063|FD-Score:3.39|P-value:1.09E-4 ID:SGTC_1630|Compound:st005209|FD-Score:4.08|P-value:4.33E-6 ID:SGTC_1672|Compound:st016598|FD-Score:3.86|P-value:1.28E-5 ID:SGTC_1832|Compound:st054113|FD-Score:3.49|P-value:6.90E-5 ID:SGTC_1916|Compound:st067606|FD-Score:4.12|P-value:3.62E-6 ID:SGTC_2116|Compound:5511452|FD-Score:3.10|P-value:3.51E-4 ID:SGTC_2141|Compound:5621638|FD-Score:3.33|P-value:1.38E-4 ID:SGTC_2143|Compound:5623677|FD-Score:3.27|P-value:1.81E-4 ID:SGTC_2161|Compound:5662504|FD-Score:-2.92|P-value:8.23E-4 ID:SGTC_2215|Compound:7188065|FD-Score:3.13|P-value:3.16E-4 ID:SGTC_2238|Compound:6671620|FD-Score:-3.32|P-value:1.76E-4 ID:SGTC_2329|Compound:6138490|FD-Score:3.36|P-value:1.21E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-3.02|P-value:5.72E-4 ID:SGTC_2412|Compound:st077135|FD-Score:3.73|P-value:2.40E-5 ID:SGTC_2416|Compound:tofa|FD-Score:4.38|P-value:8.86E-7 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:7.19|P-value:2.71E-15 ID:SGTC_2428|Compound:5615643|FD-Score:-3.47|P-value:9.37E-5 ID:SGTC_2431|Compound:5490079|FD-Score:7.11|P-value:5.11E-15 ID:SGTC_2478|Compound:5763493|FD-Score:3.52|P-value:6.07E-5 ID:SGTC_2514|Compound:salinomycin|FD-Score:2.91|P-value:7.29E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:14.30|P-value:1.68E-54 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:-5.06|P-value:2.34E-8 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:7.47|P-value:2.25E-16 ID:SGTC_2641|Compound:levomentholum|FD-Score:4.10|P-value:3.94E-6 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:-2.90|P-value:8.87E-4 ID:SGTC_3213|Compound:9129884|FD-Score:3.26|P-value:1.87E-4 ID:SGTC_3221|Compound:9128209|FD-Score:-3.27|P-value:2.17E-4 ID:SGTC_3223|Compound:9129970|FD-Score:3.13|P-value:3.11E-4 ID:SGTC_3235|Compound:9132602|FD-Score:4.10|P-value:3.84E-6 ID:SGTC_3244|Compound:9134489|FD-Score:-2.90|P-value:9.09E-4 ID:SGTC_3252|Compound:9135871|FD-Score:-3.06|P-value:5.00E-4 ID:SGTC_3312|Compound:9133330|FD-Score:3.45|P-value:8.38E-5 ID:SGTC_176|Compound:1486-1328|FD-Score:4.74|P-value:1.22E-7 ID:SGTC_180|Compound:1486-1293|FD-Score:4.00|P-value:6.63E-6 ID:SGTC_182|Compound:3448-4371|FD-Score:4.80|P-value:8.30E-8 ID:SGTC_209|Compound:4077-0173|FD-Score:3.03|P-value:4.76E-4 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:2.88|P-value:8.41E-4 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.71|P-value:2.61E-5 ID:SGTC_848|Compound:0368-0089|FD-Score:3.07|P-value:4.02E-4 ID:SGTC_862|Compound:0452-0056|FD-Score:2.86|P-value:8.83E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:3.66|P-value:3.33E-5 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:5.54|P-value:8.28E-10 ID:SGTC_1098|Compound:clofazimine|FD-Score:3.43|P-value:9.11E-5 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:4.28|P-value:1.53E-6 ID:SGTC_1204|Compound:4049-0315|FD-Score:-4.73|P-value:1.66E-7 ID:SGTC_1246|Compound:0422-0085|FD-Score:3.13|P-value:3.13E-4 ID:SGTC_1256|Compound:0570-0116|FD-Score:-3.06|P-value:4.93E-4 ID:SGTC_1365|Compound:1672-3266|FD-Score:3.57|P-value:4.96E-5 ID:SGTC_1404|Compound:3909-7820|FD-Score:3.25|P-value:1.93E-4 ID:SGTC_1491|Compound:4138-0063|FD-Score:3.39|P-value:1.09E-4 ID:SGTC_1630|Compound:st005209|FD-Score:4.08|P-value:4.33E-6 ID:SGTC_1672|Compound:st016598|FD-Score:3.86|P-value:1.28E-5 ID:SGTC_1832|Compound:st054113|FD-Score:3.49|P-value:6.90E-5 ID:SGTC_1916|Compound:st067606|FD-Score:4.12|P-value:3.62E-6 ID:SGTC_2116|Compound:5511452|FD-Score:3.10|P-value:3.51E-4 ID:SGTC_2141|Compound:5621638|FD-Score:3.33|P-value:1.38E-4 ID:SGTC_2143|Compound:5623677|FD-Score:3.27|P-value:1.81E-4 ID:SGTC_2161|Compound:5662504|FD-Score:-2.92|P-value:8.23E-4 ID:SGTC_2215|Compound:7188065|FD-Score:3.13|P-value:3.16E-4 ID:SGTC_2238|Compound:6671620|FD-Score:-3.32|P-value:1.76E-4 ID:SGTC_2329|Compound:6138490|FD-Score:3.36|P-value:1.21E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-3.02|P-value:5.72E-4 ID:SGTC_2412|Compound:st077135|FD-Score:3.73|P-value:2.40E-5 ID:SGTC_2416|Compound:tofa|FD-Score:4.38|P-value:8.86E-7 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:7.19|P-value:2.71E-15 ID:SGTC_2428|Compound:5615643|FD-Score:-3.47|P-value:9.37E-5 ID:SGTC_2431|Compound:5490079|FD-Score:7.11|P-value:5.11E-15 ID:SGTC_2478|Compound:5763493|FD-Score:3.52|P-value:6.07E-5 ID:SGTC_2514|Compound:salinomycin|FD-Score:2.91|P-value:7.29E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:14.30|P-value:1.68E-54 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:-5.06|P-value:2.34E-8 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:7.47|P-value:2.25E-16 ID:SGTC_2641|Compound:levomentholum|FD-Score:4.10|P-value:3.94E-6 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:-2.90|P-value:8.87E-4 ID:SGTC_3213|Compound:9129884|FD-Score:3.26|P-value:1.87E-4 ID:SGTC_3221|Compound:9128209|FD-Score:-3.27|P-value:2.17E-4 ID:SGTC_3223|Compound:9129970|FD-Score:3.13|P-value:3.11E-4 ID:SGTC_3235|Compound:9132602|FD-Score:4.10|P-value:3.84E-6 ID:SGTC_3244|Compound:9134489|FD-Score:-2.90|P-value:9.09E-4 ID:SGTC_3252|Compound:9135871|FD-Score:-3.06|P-value:5.00E-4 ID:SGTC_3312|Compound:9133330|FD-Score:3.45|P-value:8.38E-5

Top fitness defect scores for YNR032C-A deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2563 tomatine 14.30 8.5 µM 1.68E-54 1 1.58E-46
2 SGTC_2567 aureobasidin a 7.47 60.0 nM 2.25E-16 25 4.00E-14
3 SGTC_2420 aureobasidin a 7.19 50.0 nM 2.71E-15 9 3.32E-13
4 SGTC_2431 5490079 7.11 200.0 µM 5.11E-15 3 5.68E-13
5 SGTC_1083 didemethylchlorpromazine sulfoxide 5.54 707.4 nM 8.28E-10 redox potentiating 35 1.53E-8
6 SGTC_182 3448-4371 4.80 550.5 µM 8.30E-8 14 7.74E-7
7 SGTC_176 1486-1328 4.74 72.8 µM 1.22E-7 35 1.08E-6
8 SGTC_2416 tofa 4.38 880.0 nM 8.86E-7 sphingolipid biosynthesis & PDR1 37 5.81E-6
9 SGTC_1135 dihydrocapsaicin 4.28 536.7 nM 1.53E-6 26 9.26E-6
10 SGTC_1916 st067606 4.12 49.2 µM 3.62E-6 33 1.93E-5
11 SGTC_3235 9132602 4.10 49.5 µM 3.84E-6 21 2.03E-5
12 SGTC_2641 levomentholum 4.10 100.0 µM 3.94E-6 5 2.07E-5
13 SGTC_1630 st005209 4.08 31.3 µM 4.33E-6 23 2.25E-5
14 SGTC_180 1486-1293 4.00 102.8 µM 6.63E-6 41 3.23E-5
15 SGTC_1672 st016598 3.86 6.5 µM 1.28E-5 endomembrane recycling 71 5.67E-5
16 SGTC_2412 st077135 3.73 33.4 µM 2.40E-5 31 9.67E-5
17 SGTC_243 5hr heat shock (37°C) + splitomicin 3.71 1.0 µM 2.61E-5 heat shock/prefoldin 60 1.04E-4
18 SGTC_996 3970-0799 3.66 1.8 µM 3.33E-5 100 1.28E-4
19 SGTC_1365 1672-3266 3.57 55.4 µM 4.96E-5 33 1.80E-4
20 SGTC_2478 5763493 3.52 32.3 µM 6.07E-5 34 2.14E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.307 6.12E-74 YDR367W KEI1 het Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
0.230 1.86E-41 YKL004W AUR1 het Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
0.174 2.91E-24 YGL209W MIG2 hom Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced repression of many genes; under low glucose conditions Mig2p relocalizes to mitochondrion, where it interacts with Ups1p and antagonizes mitochondrial fission factor, Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth along with Mig2p in response to glucose depletion; MIG2 has a paralog, MIG3, that arose from the whole genome duplication
0.172 8.57E-24 YDR299W BFR2 het Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock
0.161 5.16E-21 YBR145W ADH5 hom Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication
0.161 7.62E-21 YPL251W_d YPL251W_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
0.160 1.16E-20 YNL212W VID27 hom Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth
0.155 1.38E-19 YMR011W HXT2 hom High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose
0.150 2.67E-18 YJR023C_d YJR023C_d het Putative protein of unknown function; open reading frame overlaps LSM8/YJR022W encoding an essential snRNP protein required for RNA processing and splicing
0.147 1.22E-17 YDR534C FIT1 hom Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall
0.141 2.49E-16 YGR153W_p YGR153W_p hom Putative protein of unknown function
0.137 1.42E-15 YER156C_p YER156C_p hom Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1
0.135 5.06E-15 YDR328C SKP1 het Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress
0.133 9.09E-15 YPL252C YAH1 het Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
0.132 1.88E-14 YIL036W CST6 hom Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; CST6 has a paralog, ACA1, that arose from the whole genome duplication