YNR046W / TRM112

Protein involved in methylation of tRNA, rRNA, and translation factors; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; interacts with and stabilizes 18S rRNA methyltransferase Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; deletion confers resistance to zymocin; relative distribution to the nucleus increases upon DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YNR046W / TRM112

Click on Significant Values for Screen Details ID:SGTC_10|Compound:0279-0212|FD-Score:3.26|P-value:1.30E-4 ID:SGTC_60|Compound:0986-0278|FD-Score:2.77|P-value:9.68E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:4.56|P-value:1.49E-7 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:2.94|P-value:4.98E-4 ID:SGTC_282|Compound:k297-0036|FD-Score:-3.84|P-value:6.10E-6 ID:SGTC_296|Compound:1222-0039|FD-Score:3.47|P-value:4.93E-5 ID:SGTC_309|Compound:k408-0051|FD-Score:-3.59|P-value:2.18E-5 ID:SGTC_418|Compound:lividomycin a|FD-Score:3.05|P-value:3.14E-4 ID:SGTC_577|Compound:r071-0003|FD-Score:2.94|P-value:4.88E-4 ID:SGTC_579|Compound:4491-1009|FD-Score:2.91|P-value:5.60E-4 ID:SGTC_626|Compound:0214-0009|FD-Score:-2.76|P-value:8.09E-4 ID:SGTC_797|Compound:k781-0471|FD-Score:4.43|P-value:3.21E-7 ID:SGTC_846|Compound:0335-0850|FD-Score:3.03|P-value:3.46E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:2.76|P-value:9.93E-4 ID:SGTC_1001|Compound:4048-4355|FD-Score:3.74|P-value:1.34E-5 ID:SGTC_1002|Compound:4049-0206|FD-Score:2.90|P-value:5.76E-4 ID:SGTC_1004|Compound:4084-0029|FD-Score:3.01|P-value:3.76E-4 ID:SGTC_1008|Compound:4106-0037|FD-Score:2.85|P-value:7.14E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:-2.98|P-value:3.39E-4 ID:SGTC_1155|Compound:4088-0394|FD-Score:3.97|P-value:4.14E-6 ID:SGTC_1204|Compound:4049-0315|FD-Score:7.61|P-value:5.08E-18 ID:SGTC_1212|Compound:0520-0165|FD-Score:-4.10|P-value:1.49E-6 ID:SGTC_1375|Compound:2910-0797|FD-Score:3.03|P-value:3.40E-4 ID:SGTC_1445|Compound:4239-0156|FD-Score:4.31|P-value:6.35E-7 ID:SGTC_1446|Compound:4239-0190|FD-Score:3.05|P-value:3.20E-4 ID:SGTC_1469|Compound:k284-2957|FD-Score:-3.54|P-value:2.68E-5 ID:SGTC_1562|Compound:morin hydrate|FD-Score:3.36|P-value:8.26E-5 ID:SGTC_1644|Compound:st010134|FD-Score:3.10|P-value:2.57E-4 ID:SGTC_1764|Compound:st044502|FD-Score:3.09|P-value:2.62E-4 ID:SGTC_1769|Compound:st044406|FD-Score:4.12|P-value:1.80E-6 ID:SGTC_1825|Compound:st052143|FD-Score:4.20|P-value:1.22E-6 ID:SGTC_1846|Compound:st056244|FD-Score:5.32|P-value:1.12E-9 ID:SGTC_1857|Compound:st056007|FD-Score:-3.26|P-value:1.01E-4 ID:SGTC_1925|Compound:st057770|FD-Score:-3.35|P-value:6.72E-5 ID:SGTC_1930|Compound:st074634|FD-Score:2.85|P-value:6.99E-4 ID:SGTC_2009|Compound:4012297|FD-Score:3.21|P-value:1.57E-4 ID:SGTC_2306|Compound:7482397|FD-Score:-3.33|P-value:7.36E-5 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-4.25|P-value:6.58E-7 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.62|P-value:2.50E-5 ID:SGTC_2905|Compound:7799065|FD-Score:-3.98|P-value:2.96E-6 ID:SGTC_10|Compound:0279-0212|FD-Score:3.26|P-value:1.30E-4 ID:SGTC_60|Compound:0986-0278|FD-Score:2.77|P-value:9.68E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:4.56|P-value:1.49E-7 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:2.94|P-value:4.98E-4 ID:SGTC_282|Compound:k297-0036|FD-Score:-3.84|P-value:6.10E-6 ID:SGTC_296|Compound:1222-0039|FD-Score:3.47|P-value:4.93E-5 ID:SGTC_309|Compound:k408-0051|FD-Score:-3.59|P-value:2.18E-5 ID:SGTC_418|Compound:lividomycin a|FD-Score:3.05|P-value:3.14E-4 ID:SGTC_577|Compound:r071-0003|FD-Score:2.94|P-value:4.88E-4 ID:SGTC_579|Compound:4491-1009|FD-Score:2.91|P-value:5.60E-4 ID:SGTC_626|Compound:0214-0009|FD-Score:-2.76|P-value:8.09E-4 ID:SGTC_797|Compound:k781-0471|FD-Score:4.43|P-value:3.21E-7 ID:SGTC_846|Compound:0335-0850|FD-Score:3.03|P-value:3.46E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:2.76|P-value:9.93E-4 ID:SGTC_1001|Compound:4048-4355|FD-Score:3.74|P-value:1.34E-5 ID:SGTC_1002|Compound:4049-0206|FD-Score:2.90|P-value:5.76E-4 ID:SGTC_1004|Compound:4084-0029|FD-Score:3.01|P-value:3.76E-4 ID:SGTC_1008|Compound:4106-0037|FD-Score:2.85|P-value:7.14E-4 ID:SGTC_1131|Compound:3454-3128|FD-Score:-2.98|P-value:3.39E-4 ID:SGTC_1155|Compound:4088-0394|FD-Score:3.97|P-value:4.14E-6 ID:SGTC_1204|Compound:4049-0315|FD-Score:7.61|P-value:5.08E-18 ID:SGTC_1212|Compound:0520-0165|FD-Score:-4.10|P-value:1.49E-6 ID:SGTC_1375|Compound:2910-0797|FD-Score:3.03|P-value:3.40E-4 ID:SGTC_1445|Compound:4239-0156|FD-Score:4.31|P-value:6.35E-7 ID:SGTC_1446|Compound:4239-0190|FD-Score:3.05|P-value:3.20E-4 ID:SGTC_1469|Compound:k284-2957|FD-Score:-3.54|P-value:2.68E-5 ID:SGTC_1562|Compound:morin hydrate|FD-Score:3.36|P-value:8.26E-5 ID:SGTC_1644|Compound:st010134|FD-Score:3.10|P-value:2.57E-4 ID:SGTC_1764|Compound:st044502|FD-Score:3.09|P-value:2.62E-4 ID:SGTC_1769|Compound:st044406|FD-Score:4.12|P-value:1.80E-6 ID:SGTC_1825|Compound:st052143|FD-Score:4.20|P-value:1.22E-6 ID:SGTC_1846|Compound:st056244|FD-Score:5.32|P-value:1.12E-9 ID:SGTC_1857|Compound:st056007|FD-Score:-3.26|P-value:1.01E-4 ID:SGTC_1925|Compound:st057770|FD-Score:-3.35|P-value:6.72E-5 ID:SGTC_1930|Compound:st074634|FD-Score:2.85|P-value:6.99E-4 ID:SGTC_2009|Compound:4012297|FD-Score:3.21|P-value:1.57E-4 ID:SGTC_2306|Compound:7482397|FD-Score:-3.33|P-value:7.36E-5 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-4.25|P-value:6.58E-7 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.62|P-value:2.50E-5 ID:SGTC_2905|Compound:7799065|FD-Score:-3.98|P-value:2.96E-6

Top fitness defect scores for YNR046W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1204 4049-0315 7.61 18.3 µM 5.08E-18 1 1.39E-14 1.13
2 SGTC_1846 st056244 5.32 14.8 µM 1.12E-9 1 5.32E-8 1.03
3 SGTC_149 3970-0822 4.56 46.1 µM 1.49E-7 tubulin folding & SWR complex 6 2.56E-6 0.07
4 SGTC_797 k781-0471 4.43 270.0 µM 3.21E-7 RPP1 & pyrimidine depletion 1 4.70E-6 0.76
5 SGTC_1445 4239-0156 4.31 97.7 µM 6.35E-7 3 8.08E-6 0.50
6 SGTC_1825 st052143 4.20 62.6 µM 1.22E-6 3 1.36E-5 0.33
7 SGTC_1769 st044406 4.12 64.4 µM 1.80E-6 9 1.85E-5 0.02
8 SGTC_1155 4088-0394 3.97 48.2 µM 4.14E-6 4 3.59E-5 0.25
9 SGTC_1001 4048-4355 3.74 44.4 µM 1.34E-5 copper-dependent oxidative stress 13 9.13E-5 0.29
10 SGTC_2567 aureobasidin a 3.62 60.0 nM 2.50E-5 50 1.50E-4 0.02
11 SGTC_296 1222-0039 3.47 74.9 µM 4.93E-5 2 2.59E-4 0.02
12 SGTC_1562 morin hydrate 3.36 59.1 µM 8.26E-5 12 3.90E-4 0.09
13 SGTC_10 0279-0212 3.26 42.6 µM 1.30E-4 TRP & mitochondrial translation 10 5.61E-4 0.08
14 SGTC_2009 4012297 3.21 12.1 µM 1.57E-4 cell wall signaling 14 6.53E-4 0.06
15 SGTC_1644 st010134 3.10 20.9 µM 2.57E-4 mitochondrial processes 10 9.67E-4 0.07
16 SGTC_1764 st044502 3.09 93.5 µM 2.62E-4 13 9.84E-4 0.07
17 SGTC_418 lividomycin a 3.05 100.0 µM 3.14E-4 8 0.00114 0.02
18 SGTC_1446 4239-0190 3.05 62.0 µM 3.20E-4 17 0.00115 0.03
19 SGTC_1375 2910-0797 3.03 186.0 µM 3.40E-4 13 0.00121 0.01
20 SGTC_846 0335-0850 3.03 148.0 µM 3.46E-4 ubiquinone biosynthesis & proteasome 11 0.00123 0.03

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.181 3.41E-26 YDL182W LYS20 hom Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS20 has a paralog, LYS21, that arose from the whole genome duplication
0.161 5.10E-21 YKL004W AUR1 het Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
0.144 4.65E-17 YAL032C PRP45 het Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene
0.118 5.89E-12 YDR367W KEI1 het Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
0.113 4.68E-11 YDR118W APC4 het Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress
0.113 4.84E-11 YJR072C NPA3 het Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress
0.107 5.76E-10 YAL003W EFB1 het Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site
0.105 9.89E-10 YOR011W AUS1 hom Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen
0.103 2.11E-9 YGR140W CBF2 het Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress
0.102 2.89E-9 YPL084W BRO1 hom Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes
0.100 6.85E-9 YHR147C MRPL6 hom Mitochondrial ribosomal protein of the large subunit
0.099 9.95E-9 YGL207W SPT16 het Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome
0.097 1.56E-8 YKR051W_p YKR051W_p hom Putative protein of unknown function
0.097 1.68E-8 YBR155W CNS1 het TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion
0.086 5.38E-7 YOR280C FSH3 hom Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2