YNR050C / LYS9

Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112

Zygosity: Homozygous strain
fixedexpanded
Profile for YNR050C / LYS9

Click on Significant Values for Screen Details ID:SGTC_64|Compound:000l-0018|FD-Score:-3.19|P-value:8.11E-4 ID:SGTC_114|Compound:0364-0001|FD-Score:4.33|P-value:1.15E-5 ID:SGTC_210|Compound:3551-0445|FD-Score:4.28|P-value:1.40E-5 ID:SGTC_221|Compound:trifluoperazine|FD-Score:4.07|P-value:3.45E-5 ID:SGTC_253|Compound:dyclonine|FD-Score:6.27|P-value:4.25E-10 ID:SGTC_285|Compound:1486-1325|FD-Score:3.51|P-value:3.03E-4 ID:SGTC_307|Compound:1501-1223|FD-Score:4.27|P-value:1.47E-5 ID:SGTC_360|Compound:0957-0399|FD-Score:-4.12|P-value:2.41E-5 ID:SGTC_363|Compound:0455-0021|FD-Score:3.66|P-value:1.77E-4 ID:SGTC_400|Compound:oxethazaine|FD-Score:3.24|P-value:7.80E-4 ID:SGTC_444|Compound:curcumin|FD-Score:-3.18|P-value:8.38E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.44|P-value:6.95E-6 ID:SGTC_735|Compound:3076-0345|FD-Score:5.56|P-value:2.60E-8 ID:SGTC_811|Compound:0416-0114|FD-Score:3.80|P-value:1.04E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:4.11|P-value:2.96E-5 ID:SGTC_1033|Compound:k072-0102|FD-Score:-3.27|P-value:6.20E-4 ID:SGTC_1042|Compound:0350-0096|FD-Score:-3.52|P-value:2.59E-4 ID:SGTC_1229|Compound:0139-0193|FD-Score:3.42|P-value:4.13E-4 ID:SGTC_1234|Compound:0326-0494|FD-Score:4.63|P-value:3.01E-6 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:5.78|P-value:7.44E-9 ID:SGTC_1729|Compound:st035615|FD-Score:4.16|P-value:2.33E-5 ID:SGTC_1786|Compound:berberine|FD-Score:4.11|P-value:2.87E-5 ID:SGTC_1835|Compound:st008666|FD-Score:4.12|P-value:2.76E-5 ID:SGTC_1978|Compound:st071761|FD-Score:-3.88|P-value:6.52E-5 ID:SGTC_1984|Compound:st072354|FD-Score:3.41|P-value:4.32E-4 ID:SGTC_1999|Compound:st074831|FD-Score:3.32|P-value:5.90E-4 ID:SGTC_2272|Compound:7805186|FD-Score:-3.21|P-value:7.63E-4 ID:SGTC_2330|Compound:7979373|FD-Score:3.98|P-value:4.98E-5 ID:SGTC_2398|Compound:5228213|FD-Score:-3.23|P-value:7.19E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:3.84|P-value:8.78E-5 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:3.43|P-value:4.08E-4 ID:SGTC_2417|Compound:cerulenin|FD-Score:5.85|P-value:5.22E-9 ID:SGTC_2421|Compound:5690543|FD-Score:5.70|P-value:1.18E-8 ID:SGTC_2430|Compound:5571206|FD-Score:4.86|P-value:9.84E-7 ID:SGTC_2433|Compound:5607874|FD-Score:-3.69|P-value:1.32E-4 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:3.49|P-value:3.30E-4 ID:SGTC_2629|Compound:bisabolol|FD-Score:3.54|P-value:2.70E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:3.42|P-value:4.17E-4 ID:SGTC_2783|Compound:7738165|FD-Score:-12.10|P-value:5.02E-33 ID:SGTC_2788|Compound:5282734|FD-Score:-3.43|P-value:3.48E-4 ID:SGTC_3056|Compound:9094900|FD-Score:3.82|P-value:9.56E-5 ID:SGTC_3093|Compound:9117344|FD-Score:5.74|P-value:9.36E-9 ID:SGTC_3126|Compound:9127922|FD-Score:7.26|P-value:5.87E-13 ID:SGTC_3151|Compound:9099821|FD-Score:-3.15|P-value:9.20E-4 ID:SGTC_3348|Compound:9149464|FD-Score:3.42|P-value:4.11E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.19|P-value:8.11E-4 ID:SGTC_114|Compound:0364-0001|FD-Score:4.33|P-value:1.15E-5 ID:SGTC_210|Compound:3551-0445|FD-Score:4.28|P-value:1.40E-5 ID:SGTC_221|Compound:trifluoperazine|FD-Score:4.07|P-value:3.45E-5 ID:SGTC_253|Compound:dyclonine|FD-Score:6.27|P-value:4.25E-10 ID:SGTC_285|Compound:1486-1325|FD-Score:3.51|P-value:3.03E-4 ID:SGTC_307|Compound:1501-1223|FD-Score:4.27|P-value:1.47E-5 ID:SGTC_360|Compound:0957-0399|FD-Score:-4.12|P-value:2.41E-5 ID:SGTC_363|Compound:0455-0021|FD-Score:3.66|P-value:1.77E-4 ID:SGTC_400|Compound:oxethazaine|FD-Score:3.24|P-value:7.80E-4 ID:SGTC_444|Compound:curcumin|FD-Score:-3.18|P-value:8.38E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.44|P-value:6.95E-6 ID:SGTC_735|Compound:3076-0345|FD-Score:5.56|P-value:2.60E-8 ID:SGTC_811|Compound:0416-0114|FD-Score:3.80|P-value:1.04E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:4.11|P-value:2.96E-5 ID:SGTC_1033|Compound:k072-0102|FD-Score:-3.27|P-value:6.20E-4 ID:SGTC_1042|Compound:0350-0096|FD-Score:-3.52|P-value:2.59E-4 ID:SGTC_1229|Compound:0139-0193|FD-Score:3.42|P-value:4.13E-4 ID:SGTC_1234|Compound:0326-0494|FD-Score:4.63|P-value:3.01E-6 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:5.78|P-value:7.44E-9 ID:SGTC_1729|Compound:st035615|FD-Score:4.16|P-value:2.33E-5 ID:SGTC_1786|Compound:berberine|FD-Score:4.11|P-value:2.87E-5 ID:SGTC_1835|Compound:st008666|FD-Score:4.12|P-value:2.76E-5 ID:SGTC_1978|Compound:st071761|FD-Score:-3.88|P-value:6.52E-5 ID:SGTC_1984|Compound:st072354|FD-Score:3.41|P-value:4.32E-4 ID:SGTC_1999|Compound:st074831|FD-Score:3.32|P-value:5.90E-4 ID:SGTC_2272|Compound:7805186|FD-Score:-3.21|P-value:7.63E-4 ID:SGTC_2330|Compound:7979373|FD-Score:3.98|P-value:4.98E-5 ID:SGTC_2398|Compound:5228213|FD-Score:-3.23|P-value:7.19E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:3.84|P-value:8.78E-5 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:3.43|P-value:4.08E-4 ID:SGTC_2417|Compound:cerulenin|FD-Score:5.85|P-value:5.22E-9 ID:SGTC_2421|Compound:5690543|FD-Score:5.70|P-value:1.18E-8 ID:SGTC_2430|Compound:5571206|FD-Score:4.86|P-value:9.84E-7 ID:SGTC_2433|Compound:5607874|FD-Score:-3.69|P-value:1.32E-4 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:3.49|P-value:3.30E-4 ID:SGTC_2629|Compound:bisabolol|FD-Score:3.54|P-value:2.70E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:3.42|P-value:4.17E-4 ID:SGTC_2783|Compound:7738165|FD-Score:-12.10|P-value:5.02E-33 ID:SGTC_2788|Compound:5282734|FD-Score:-3.43|P-value:3.48E-4 ID:SGTC_3056|Compound:9094900|FD-Score:3.82|P-value:9.56E-5 ID:SGTC_3093|Compound:9117344|FD-Score:5.74|P-value:9.36E-9 ID:SGTC_3126|Compound:9127922|FD-Score:7.26|P-value:5.87E-13 ID:SGTC_3151|Compound:9099821|FD-Score:-3.15|P-value:9.20E-4 ID:SGTC_3348|Compound:9149464|FD-Score:3.42|P-value:4.11E-4

Top fitness defect scores for YNR050C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3126 9127922 7.26 49.5 µM 5.87E-13 fatty acid desaturase (OLE1) 12 1.99E-13
2 SGTC_253 dyclonine 6.27 31.2 µM 4.25E-10 amide catabolism 8 1.86E-10
3 SGTC_2417 cerulenin 5.85 830.0 nM 5.22E-9 8 2.52E-9
4 SGTC_1557 sumaresinolic acid 5.78 42.3 µM 7.44E-9 2 3.64E-9
5 SGTC_3093 9117344 5.74 49.5 µM 9.36E-9 60S ribosome export 3 4.62E-9
6 SGTC_2421 5690543 5.70 90.7 µM 1.18E-8 5 5.87E-9
7 SGTC_735 3076-0345 5.56 636.0 µM 2.60E-8 6 1.34E-8
8 SGTC_2430 5571206 4.86 200.0 µM 9.84E-7 35 5.82E-7
9 SGTC_1234 0326-0494 4.63 15.5 µM 3.01E-6 8 1.86E-6
10 SGTC_682 0kpi-0099 4.44 17.0 µM 6.95E-6 53 4.44E-6
11 SGTC_114 0364-0001 4.33 490.3 µM 1.15E-5 36 7.50E-6
12 SGTC_210 3551-0445 4.28 56.8 µM 1.40E-5 54 9.18E-6
13 SGTC_307 1501-1223 4.27 22.3 µM 1.47E-5 8 9.64E-6
14 SGTC_1729 st035615 4.16 46.0 µM 2.33E-5 33 1.56E-5
15 SGTC_1835 st008666 4.12 49.2 µM 2.76E-5 22 1.86E-5
16 SGTC_1786 berberine 4.11 21.6 µM 2.87E-5 60S ribosome export 27 1.94E-5
17 SGTC_946 3434-1334 4.11 87.4 µM 2.96E-5 26 2.00E-5
18 SGTC_221 trifluoperazine 4.07 10.3 µM 3.45E-5 NEO1-PIK1 202 2.35E-5
19 SGTC_2330 7979373 3.98 200.0 µM 4.98E-5 60S ribosome export 33 3.43E-5
20 SGTC_2406 ethylparaben 3.84 405.7 µM 8.78E-5 44 6.19E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.175 2.05E-24 YLR243W GPN3 het Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p
0.114 3.57E-11 YML126C ERG13 het 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
0.106 7.52E-10 YNL071W LAT1 hom Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA
0.104 1.32E-9 YHR132W-A IGO2 hom Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo1p
0.100 6.78E-9 YGL055W OLE1 het Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
0.093 7.28E-8 YGL097W SRM1 het Nucleotide exchange factor for Gsp1p, localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p
0.092 8.19E-8 YLR232W_d YLR232W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene BNA5
0.092 1.08E-7 YOR083W WHI5 hom Repressor of G1 transcription that binds to SCB binding factor (SBF) at SCB target promoters in early G1; phosphorylation of Whi5p by the CDK, Cln3p/Cdc28p relieves repression and promoter binding by Whi5; periodically expressed in G1
0.091 1.19E-7 YGL077C HNM1 hom Choline/ethanolamine transporter; involved in the uptake of nitrogen mustard and the uptake of glycine betaine during hypersaline stress; co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol
0.088 3.25E-7 YHL011C PRS3 hom 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes
0.086 6.36E-7 YKL081W TEF4 hom Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex
0.086 6.47E-7 YNL116W DMA2 hom Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma1p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8 protein, with sequence similarity to human Chfr; contains FHA and RING finger domains
0.085 8.77E-7 YNL295W_p YNL295W_p hom Putative protein of unknown function
0.084 1.21E-6 YOL159C YOL159C hom Soluble protein of unknown function; deletion mutants are viable and have elevated levels of Ty1 retrotransposition and Ty1 cDNA
0.083 1.32E-6 YOR357C SNX3 hom Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p