YNR054C / ESF2

Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome

Zygosity: Heterozygous strain
fixedexpanded
Profile for YNR054C / ESF2

Click on Significant Values for Screen Details ID:SGTC_109|Compound:0132-0036|FD-Score:-2.93|P-value:7.68E-4 ID:SGTC_113|Compound:0099-0221|FD-Score:-2.99|P-value:6.15E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:3.87|P-value:2.06E-5 ID:SGTC_213|Compound:1246-0773|FD-Score:-2.99|P-value:6.04E-4 ID:SGTC_404|Compound:fumagillin|FD-Score:3.25|P-value:2.83E-4 ID:SGTC_484|Compound:loperamide|FD-Score:2.93|P-value:9.60E-4 ID:SGTC_500|Compound:h-89|FD-Score:4.19|P-value:4.25E-6 ID:SGTC_572|Compound:0960-0087|FD-Score:3.00|P-value:7.39E-4 ID:SGTC_577|Compound:r071-0003|FD-Score:2.95|P-value:8.96E-4 ID:SGTC_664|Compound:1222-0054|FD-Score:3.85|P-value:2.24E-5 ID:SGTC_680|Compound:0417-1641|FD-Score:3.54|P-value:8.77E-5 ID:SGTC_955|Compound:1216-0110|FD-Score:3.42|P-value:1.48E-4 ID:SGTC_1045|Compound:1348-1485|FD-Score:2.96|P-value:8.55E-4 ID:SGTC_1139|Compound:k015-0024|FD-Score:-3.69|P-value:3.49E-5 ID:SGTC_1220|Compound:1838-0075|FD-Score:3.04|P-value:6.44E-4 ID:SGTC_1321|Compound:1326-2144|FD-Score:-3.19|P-value:2.80E-4 ID:SGTC_1492|Compound:4158-0558|FD-Score:3.13|P-value:4.59E-4 ID:SGTC_1512|Compound:0259-0544|FD-Score:3.72|P-value:3.91E-5 ID:SGTC_1541|Compound:2',3',6-trimethoxyflavone|FD-Score:3.04|P-value:6.47E-4 ID:SGTC_1660|Compound:st012960|FD-Score:-3.74|P-value:2.69E-5 ID:SGTC_1666|Compound:st014829|FD-Score:3.19|P-value:3.59E-4 ID:SGTC_1738|Compound:st035555|FD-Score:3.76|P-value:3.28E-5 ID:SGTC_1916|Compound:st067606|FD-Score:4.16|P-value:5.10E-6 ID:SGTC_1917|Compound:st066507|FD-Score:2.95|P-value:8.99E-4 ID:SGTC_1920|Compound:st075728|FD-Score:-5.29|P-value:6.35E-9 ID:SGTC_1925|Compound:st057770|FD-Score:-3.66|P-value:3.97E-5 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:-4.65|P-value:2.69E-7 ID:SGTC_1935|Compound:st074532|FD-Score:3.62|P-value:6.32E-5 ID:SGTC_1962|Compound:st076595|FD-Score:3.21|P-value:3.41E-4 ID:SGTC_1982|Compound:st072467|FD-Score:-3.08|P-value:4.35E-4 ID:SGTC_2028|Compound:5153890|FD-Score:3.42|P-value:1.44E-4 ID:SGTC_2063|Compound:5246145|FD-Score:3.43|P-value:1.38E-4 ID:SGTC_2328|Compound:6201408|FD-Score:4.54|P-value:7.15E-7 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:-2.93|P-value:7.55E-4 ID:SGTC_2690|Compound:usnic acid|FD-Score:2.95|P-value:9.07E-4 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:-3.82|P-value:1.86E-5 ID:SGTC_2806|Compound:7952793|FD-Score:-3.80|P-value:2.08E-5 ID:SGTC_2830|Compound:7992007|FD-Score:-2.91|P-value:8.18E-4 ID:SGTC_3193|Compound:9111931|FD-Score:-3.07|P-value:4.53E-4 ID:SGTC_109|Compound:0132-0036|FD-Score:-2.93|P-value:7.68E-4 ID:SGTC_113|Compound:0099-0221|FD-Score:-2.99|P-value:6.15E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:3.87|P-value:2.06E-5 ID:SGTC_213|Compound:1246-0773|FD-Score:-2.99|P-value:6.04E-4 ID:SGTC_404|Compound:fumagillin|FD-Score:3.25|P-value:2.83E-4 ID:SGTC_484|Compound:loperamide|FD-Score:2.93|P-value:9.60E-4 ID:SGTC_500|Compound:h-89|FD-Score:4.19|P-value:4.25E-6 ID:SGTC_572|Compound:0960-0087|FD-Score:3.00|P-value:7.39E-4 ID:SGTC_577|Compound:r071-0003|FD-Score:2.95|P-value:8.96E-4 ID:SGTC_664|Compound:1222-0054|FD-Score:3.85|P-value:2.24E-5 ID:SGTC_680|Compound:0417-1641|FD-Score:3.54|P-value:8.77E-5 ID:SGTC_955|Compound:1216-0110|FD-Score:3.42|P-value:1.48E-4 ID:SGTC_1045|Compound:1348-1485|FD-Score:2.96|P-value:8.55E-4 ID:SGTC_1139|Compound:k015-0024|FD-Score:-3.69|P-value:3.49E-5 ID:SGTC_1220|Compound:1838-0075|FD-Score:3.04|P-value:6.44E-4 ID:SGTC_1321|Compound:1326-2144|FD-Score:-3.19|P-value:2.80E-4 ID:SGTC_1492|Compound:4158-0558|FD-Score:3.13|P-value:4.59E-4 ID:SGTC_1512|Compound:0259-0544|FD-Score:3.72|P-value:3.91E-5 ID:SGTC_1541|Compound:2',3',6-trimethoxyflavone|FD-Score:3.04|P-value:6.47E-4 ID:SGTC_1660|Compound:st012960|FD-Score:-3.74|P-value:2.69E-5 ID:SGTC_1666|Compound:st014829|FD-Score:3.19|P-value:3.59E-4 ID:SGTC_1738|Compound:st035555|FD-Score:3.76|P-value:3.28E-5 ID:SGTC_1916|Compound:st067606|FD-Score:4.16|P-value:5.10E-6 ID:SGTC_1917|Compound:st066507|FD-Score:2.95|P-value:8.99E-4 ID:SGTC_1920|Compound:st075728|FD-Score:-5.29|P-value:6.35E-9 ID:SGTC_1925|Compound:st057770|FD-Score:-3.66|P-value:3.97E-5 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:-4.65|P-value:2.69E-7 ID:SGTC_1935|Compound:st074532|FD-Score:3.62|P-value:6.32E-5 ID:SGTC_1962|Compound:st076595|FD-Score:3.21|P-value:3.41E-4 ID:SGTC_1982|Compound:st072467|FD-Score:-3.08|P-value:4.35E-4 ID:SGTC_2028|Compound:5153890|FD-Score:3.42|P-value:1.44E-4 ID:SGTC_2063|Compound:5246145|FD-Score:3.43|P-value:1.38E-4 ID:SGTC_2328|Compound:6201408|FD-Score:4.54|P-value:7.15E-7 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:-2.93|P-value:7.55E-4 ID:SGTC_2690|Compound:usnic acid|FD-Score:2.95|P-value:9.07E-4 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:-3.82|P-value:1.86E-5 ID:SGTC_2806|Compound:7952793|FD-Score:-3.80|P-value:2.08E-5 ID:SGTC_2830|Compound:7992007|FD-Score:-2.91|P-value:8.18E-4 ID:SGTC_3193|Compound:9111931|FD-Score:-3.07|P-value:4.53E-4

Top fitness defect scores for YNR054C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2328 6201408 4.54 200.0 µM 7.15E-7 7 2.83E-6 0.01
2 SGTC_500 h-89 4.19 112.0 µM 4.25E-6 cell wall signaling 7 1.37E-5 0.28
3 SGTC_1916 st067606 4.16 49.2 µM 5.10E-6 5 1.61E-5 0.31
4 SGTC_151 3013-0144 3.87 2.3 µM 2.06E-5 iron homeostasis 11 5.54E-5 0.07
5 SGTC_664 1222-0054 3.85 14.9 µM 2.24E-5 sphingolipid biosynthesis & PDR1 10 5.98E-5 0.41
6 SGTC_1738 st035555 3.76 74.0 µM 3.28E-5 TSC3-RPN4 6 8.37E-5 0.14
7 SGTC_1512 0259-0544 3.72 12.2 µM 3.91E-5 7 9.78E-5 0.17
8 SGTC_1935 st074532 3.62 41.6 µM 6.32E-5 8 1.50E-4 0.10
9 SGTC_680 0417-1641 3.54 114.0 µM 8.77E-5 RPP1 & pyrimidine depletion 10 2.00E-4 0.15
10 SGTC_2063 5246145 3.43 200.0 µM 1.38E-4 10 2.99E-4 0.06
11 SGTC_2028 5153890 3.42 36.4 µM 1.44E-4 cell wall signaling 14 3.11E-4 0.03
12 SGTC_955 1216-0110 3.42 33.0 µM 1.48E-4 sphingolipid biosynthesis & PDR1 8 3.18E-4 0.01
13 SGTC_404 fumagillin 3.25 50.0 µM 2.83E-4 13 5.67E-4 0.00
14 SGTC_1962 st076595 3.21 64.5 µM 3.41E-4 redox potentiating 26 6.69E-4 0.05
15 SGTC_1666 st014829 3.19 29.0 µM 3.59E-4 calcium & mitochondrial duress 12 7.01E-4 0.19
16 SGTC_1492 4158-0558 3.13 28.5 µM 4.59E-4 60S ribosome export 6 8.72E-4 0.40
17 SGTC_1220 1838-0075 3.04 193.0 µM 6.44E-4 9 0.00118 0.15
18 SGTC_1541 2',3',6-trimethoxyflavone 3.04 64.0 µM 6.47E-4 16 0.00119 0.03
19 SGTC_572 0960-0087 3.00 17.7 µM 7.39E-4 27 0.00133 0.00
20 SGTC_1045 1348-1485 2.96 165.0 µM 8.55E-4 13 0.00152 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.152 9.90E-19 YOL050C_d YOL050C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
0.112 7.63E-11 YLR101C_d YLR101C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF ERG27/YLR100W
0.109 2.47E-10 YMR003W AIM34 hom Protein of unknown function; GFP-fusion protein localizes to the mitochondria; null mutant is viable and displays reduced frequency of mitochondrial genome loss
0.091 1.24E-7 YPL057C SUR1 hom Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication
0.090 1.98E-7 YJR047C ANB1 hom Translation elongation factor eIF-5A; previously thought to function in translation initiation; undergoes an essential hypusination modification; expressed under anaerobic conditions; ANB1 has a paralog, HYP2, that arose from the whole genome duplication
0.085 7.64E-7 YDL054C MCH1 hom Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport
0.084 9.53E-7 YLR100W ERG27 het 3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs
0.084 1.09E-6 YDR325W YCG1 het Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation
0.083 1.63E-6 YMR105C PGM2 hom Phosphoglucomutase; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; functions as the acceptor for a Glc-phosphotransferase; protein abundance increases in response to DNA replication stress
0.082 2.17E-6 YBL078C ATG8 hom Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis
0.081 2.51E-6 YMR241W YHM2 hom Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome
0.081 2.88E-6 YOR348C PUT4 hom Proline permease, required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells
0.081 2.96E-6 YJR010C-A SPC1 hom Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress
0.079 4.33E-6 YKL023W_p YKL023W_p hom Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.078 5.58E-6 YDL078C MDH3 hom Peroxisomal malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the glyoxylate cycle