YNR057C / BIO4

Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels

Zygosity: Homozygous strain
fixedexpanded
Profile for YNR057C / BIO4

Click on Significant Values for Screen Details ID:SGTC_209|Compound:4077-0173|FD-Score:3.55|P-value:3.70E-4 ID:SGTC_250|Compound:brefeldin a|FD-Score:4.17|P-value:3.65E-5 ID:SGTC_318|Compound:1488-0629|FD-Score:4.08|P-value:5.19E-5 ID:SGTC_334|Compound:1502-1024|FD-Score:-3.65|P-value:2.13E-4 ID:SGTC_394|Compound:menadione|FD-Score:3.78|P-value:1.66E-4 ID:SGTC_403|Compound:arphamenine a|FD-Score:-3.34|P-value:6.23E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:-3.75|P-value:1.45E-4 ID:SGTC_746|Compound:1095-0025|FD-Score:-4.94|P-value:9.50E-7 ID:SGTC_750|Compound:4073-0104|FD-Score:4.75|P-value:3.03E-6 ID:SGTC_921|Compound:1561-0023|FD-Score:3.43|P-value:5.60E-4 ID:SGTC_927|Compound:2425-1185|FD-Score:4.52|P-value:8.41E-6 ID:SGTC_1118|Compound:chelidonine|FD-Score:3.41|P-value:6.07E-4 ID:SGTC_1375|Compound:2910-0797|FD-Score:6.02|P-value:4.87E-9 ID:SGTC_1380|Compound:2291-0730|FD-Score:3.37|P-value:7.03E-4 ID:SGTC_1447|Compound:4239-0816|FD-Score:3.46|P-value:5.04E-4 ID:SGTC_1536|Compound:cytidine-2',3'-monophosphoric acid;  3'-cytidylic acid|FD-Score:3.41|P-value:6.00E-4 ID:SGTC_1717|Compound:st033143|FD-Score:3.92|P-value:9.69E-5 ID:SGTC_1721|Compound:st082152|FD-Score:-3.23|P-value:8.87E-4 ID:SGTC_1769|Compound:st044406|FD-Score:3.91|P-value:9.99E-5 ID:SGTC_1771|Compound:st048882|FD-Score:3.30|P-value:8.86E-4 ID:SGTC_1947|Compound:st076332|FD-Score:5.19|P-value:3.79E-7 ID:SGTC_2104|Compound:5389443|FD-Score:4.54|P-value:7.78E-6 ID:SGTC_2105|Compound:5350118|FD-Score:3.37|P-value:6.91E-4 ID:SGTC_2146|Compound:5545642|FD-Score:3.95|P-value:8.80E-5 ID:SGTC_2167|Compound:5876490|FD-Score:-8.00|P-value:6.90E-15 ID:SGTC_2241|Compound:6772625|FD-Score:3.92|P-value:9.75E-5 ID:SGTC_2246|Compound:7293507|FD-Score:-3.78|P-value:1.28E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:3.88|P-value:1.12E-4 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:3.69|P-value:2.26E-4 ID:SGTC_2832|Compound:7992762|FD-Score:4.07|P-value:5.56E-5 ID:SGTC_2944|Compound:9067346|FD-Score:4.04|P-value:6.15E-5 ID:SGTC_2950|Compound:9072895|FD-Score:5.43|P-value:1.14E-7 ID:SGTC_2982|Compound:9019404|FD-Score:-4.88|P-value:1.25E-6 ID:SGTC_3101|Compound:9120531|FD-Score:5.45|P-value:1.06E-7 ID:SGTC_3145|Compound:9085516|FD-Score:3.62|P-value:2.94E-4 ID:SGTC_3282|Compound:9108498|FD-Score:3.96|P-value:8.25E-5 ID:SGTC_3290|Compound:9121031|FD-Score:3.61|P-value:3.00E-4 ID:SGTC_3324|Compound:9138759|FD-Score:4.87|P-value:1.82E-6 ID:SGTC_3328|Compound:9140624|FD-Score:3.91|P-value:1.03E-4 ID:SGTC_209|Compound:4077-0173|FD-Score:3.55|P-value:3.70E-4 ID:SGTC_250|Compound:brefeldin a|FD-Score:4.17|P-value:3.65E-5 ID:SGTC_318|Compound:1488-0629|FD-Score:4.08|P-value:5.19E-5 ID:SGTC_334|Compound:1502-1024|FD-Score:-3.65|P-value:2.13E-4 ID:SGTC_394|Compound:menadione|FD-Score:3.78|P-value:1.66E-4 ID:SGTC_403|Compound:arphamenine a|FD-Score:-3.34|P-value:6.23E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:-3.75|P-value:1.45E-4 ID:SGTC_746|Compound:1095-0025|FD-Score:-4.94|P-value:9.50E-7 ID:SGTC_750|Compound:4073-0104|FD-Score:4.75|P-value:3.03E-6 ID:SGTC_921|Compound:1561-0023|FD-Score:3.43|P-value:5.60E-4 ID:SGTC_927|Compound:2425-1185|FD-Score:4.52|P-value:8.41E-6 ID:SGTC_1118|Compound:chelidonine|FD-Score:3.41|P-value:6.07E-4 ID:SGTC_1375|Compound:2910-0797|FD-Score:6.02|P-value:4.87E-9 ID:SGTC_1380|Compound:2291-0730|FD-Score:3.37|P-value:7.03E-4 ID:SGTC_1447|Compound:4239-0816|FD-Score:3.46|P-value:5.04E-4 ID:SGTC_1536|Compound:cytidine-2',3'-monophosphoric acid;  3'-cytidylic acid|FD-Score:3.41|P-value:6.00E-4 ID:SGTC_1717|Compound:st033143|FD-Score:3.92|P-value:9.69E-5 ID:SGTC_1721|Compound:st082152|FD-Score:-3.23|P-value:8.87E-4 ID:SGTC_1769|Compound:st044406|FD-Score:3.91|P-value:9.99E-5 ID:SGTC_1771|Compound:st048882|FD-Score:3.30|P-value:8.86E-4 ID:SGTC_1947|Compound:st076332|FD-Score:5.19|P-value:3.79E-7 ID:SGTC_2104|Compound:5389443|FD-Score:4.54|P-value:7.78E-6 ID:SGTC_2105|Compound:5350118|FD-Score:3.37|P-value:6.91E-4 ID:SGTC_2146|Compound:5545642|FD-Score:3.95|P-value:8.80E-5 ID:SGTC_2167|Compound:5876490|FD-Score:-8.00|P-value:6.90E-15 ID:SGTC_2241|Compound:6772625|FD-Score:3.92|P-value:9.75E-5 ID:SGTC_2246|Compound:7293507|FD-Score:-3.78|P-value:1.28E-4 ID:SGTC_2593|Compound:nobiletin|FD-Score:3.88|P-value:1.12E-4 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:3.69|P-value:2.26E-4 ID:SGTC_2832|Compound:7992762|FD-Score:4.07|P-value:5.56E-5 ID:SGTC_2944|Compound:9067346|FD-Score:4.04|P-value:6.15E-5 ID:SGTC_2950|Compound:9072895|FD-Score:5.43|P-value:1.14E-7 ID:SGTC_2982|Compound:9019404|FD-Score:-4.88|P-value:1.25E-6 ID:SGTC_3101|Compound:9120531|FD-Score:5.45|P-value:1.06E-7 ID:SGTC_3145|Compound:9085516|FD-Score:3.62|P-value:2.94E-4 ID:SGTC_3282|Compound:9108498|FD-Score:3.96|P-value:8.25E-5 ID:SGTC_3290|Compound:9121031|FD-Score:3.61|P-value:3.00E-4 ID:SGTC_3324|Compound:9138759|FD-Score:4.87|P-value:1.82E-6 ID:SGTC_3328|Compound:9140624|FD-Score:3.91|P-value:1.03E-4

Top fitness defect scores for YNR057C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1375 2910-0797 6.02 186.0 µM 4.87E-9 5 8.78E-10
2 SGTC_3101 9120531 5.45 49.5 µM 1.06E-7 8 2.55E-8
3 SGTC_2950 9072895 5.43 21.5 µM 1.14E-7 11 2.76E-8
4 SGTC_1947 st076332 5.19 61.6 µM 3.79E-7 5 1.03E-7
5 SGTC_3324 9138759 4.87 60.8 µM 1.82E-6 8 5.71E-7
6 SGTC_750 4073-0104 4.75 132.0 µM 3.03E-6 4 9.94E-7
7 SGTC_2104 5389443 4.54 200.0 µM 7.78E-6 5 2.78E-6
8 SGTC_927 2425-1185 4.52 123.0 µM 8.41E-6 19 3.03E-6
9 SGTC_250 brefeldin a 4.17 552.2 µM 3.65E-5 PDR1 11 1.51E-5
10 SGTC_318 1488-0629 4.08 67.2 µM 5.19E-5 copper-dependent oxidative stress 22 2.22E-5
11 SGTC_2832 7992762 4.07 52.0 µM 5.56E-5 26 2.39E-5
12 SGTC_2944 9067346 4.04 25.6 µM 6.15E-5 22 2.67E-5
13 SGTC_3282 9108498 3.96 15.5 µM 8.25E-5 30 3.68E-5
14 SGTC_2146 5545642 3.95 200.0 µM 8.80E-5 18 3.94E-5
15 SGTC_1717 st033143 3.92 58.0 µM 9.69E-5 41 4.38E-5
16 SGTC_2241 6772625 3.92 37.8 µM 9.75E-5 25 4.41E-5
17 SGTC_1769 st044406 3.91 64.4 µM 9.99E-5 16 4.53E-5
18 SGTC_3328 9140624 3.91 29.0 µM 1.03E-4 23 4.67E-5
19 SGTC_2593 nobiletin 3.88 86.2 µM 1.12E-4 28 5.14E-5
20 SGTC_394 menadione 3.78 8.3 µM 1.66E-4 superoxide 39 7.91E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.096 2.73E-8 YFR012W DCV1 hom Protein of unknown function; deletion mutant shows strong genetic interaction with cdc28-as1 mutant in the presence of 1-NM-PP1; DCV1 has a paralog, YOL019W, that arose from the whole genome duplication
0.094 4.40E-8 YIL009C-A EST3 hom Component of the telomerase holoenzyme, involved in telomere replication
0.094 5.43E-8 YMR078C CTF18 hom Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint
0.092 1.05E-7 YLR401C DUS3 hom Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress
0.083 1.37E-6 YCR004C YCP4 hom Protein of unknown function, has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.077 7.31E-6 YDL201W TRM8 hom Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
0.077 7.45E-6 YGL228W SHE10 hom Putative glycosylphosphatidylinositol (GPI)-anchored protein of unknown function; overexpression causes growth arrest
0.077 7.49E-6 YEL067C_p YEL067C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.076 9.57E-6 YOR144C ELG1 hom Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication
0.075 1.47E-5 YJL107C_p YJL107C_p hom Putative protein of unknown function; expression is induced by activation of the HOG1 mitogen-activated signaling pathway and this induction is Hog1p/Pbs2p dependent; YJL107C and adjacent ORF, YJL108C are merged in related fungi
0.073 2.49E-5 YIR022W SEC11 het 18kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum
0.072 3.26E-5 YBR105C VID24 hom GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles
0.070 4.55E-5 YDR165W TRM82 hom Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
0.070 4.72E-5 YMR020W FMS1 hom Polyamine oxidase, converts spermine to spermidine, which is required for the essential hypusination modification of translation factor eIF-5A; also involved in pantothenic acid biosynthesis
0.069 5.71E-5 YJR055W HIT1 hom Protein of unknown function, required for growth at high temperature