YNR060W / FRE4

Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels

Zygosity: Homozygous strain
fixedexpanded
Profile for YNR060W / FRE4

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:6.90|P-value:7.45E-13 ID:SGTC_76|Compound:0433-0009|FD-Score:4.21|P-value:7.87E-6 ID:SGTC_143|Compound:4032-1194|FD-Score:-3.34|P-value:3.17E-4 ID:SGTC_154|Compound:3389-1638|FD-Score:5.01|P-value:1.42E-7 ID:SGTC_176|Compound:1486-1328|FD-Score:4.71|P-value:6.79E-7 ID:SGTC_322|Compound:k048-0136|FD-Score:6.57|P-value:8.53E-12 ID:SGTC_324|Compound:1315-0088|FD-Score:3.28|P-value:3.83E-4 ID:SGTC_456|Compound:gingerol|FD-Score:3.75|P-value:6.04E-5 ID:SGTC_523|Compound:1934-0222|FD-Score:3.14|P-value:6.35E-4 ID:SGTC_536|Compound:3852-0578|FD-Score:3.94|P-value:2.63E-5 ID:SGTC_537|Compound:1334-0398|FD-Score:3.84|P-value:4.09E-5 ID:SGTC_655|Compound:k060-0012|FD-Score:3.18|P-value:5.53E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:3.42|P-value:2.26E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:3.49|P-value:1.74E-4 ID:SGTC_927|Compound:2425-1185|FD-Score:3.31|P-value:3.42E-4 ID:SGTC_938|Compound:3126-1755|FD-Score:3.57|P-value:1.26E-4 ID:SGTC_1000|Compound:4048-0884|FD-Score:3.89|P-value:3.37E-5 ID:SGTC_1252|Compound:0493-0875|FD-Score:3.12|P-value:6.86E-4 ID:SGTC_1257|Compound:0669-0121|FD-Score:3.26|P-value:4.20E-4 ID:SGTC_1283|Compound:0922-0125|FD-Score:3.48|P-value:1.79E-4 ID:SGTC_1337|Compound:1486-1011|FD-Score:3.47|P-value:1.88E-4 ID:SGTC_1451|Compound:4152-0210|FD-Score:4.14|P-value:1.08E-5 ID:SGTC_1458|Compound:k015-0034|FD-Score:4.31|P-value:5.00E-6 ID:SGTC_1540|Compound:guanosine|FD-Score:3.89|P-value:3.29E-5 ID:SGTC_1851|Compound:st056236|FD-Score:3.50|P-value:1.63E-4 ID:SGTC_2295|Compound:7971645|FD-Score:-3.73|P-value:6.73E-5 ID:SGTC_2340|Compound:7987427|FD-Score:-5.16|P-value:6.52E-8 ID:SGTC_2351|Compound:9026858|FD-Score:7.16|P-value:1.06E-13 ID:SGTC_2387|Compound:9077560|FD-Score:-3.74|P-value:6.47E-5 ID:SGTC_2393|Compound:9031399|FD-Score:4.29|P-value:5.36E-6 ID:SGTC_2395|Compound:5646831|FD-Score:5.99|P-value:4.14E-10 ID:SGTC_2400|Compound:5203494|FD-Score:4.15|P-value:1.05E-5 ID:SGTC_2405|Compound:ticlatone|FD-Score:4.28|P-value:5.64E-6 ID:SGTC_2642|Compound:cholecalciferol|FD-Score:3.15|P-value:6.26E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:3.65|P-value:9.04E-5 ID:SGTC_2777|Compound:climbazole|FD-Score:3.81|P-value:4.72E-5 ID:SGTC_2788|Compound:5282734|FD-Score:3.39|P-value:2.49E-4 ID:SGTC_2798|Compound:7813637|FD-Score:3.93|P-value:2.83E-5 ID:SGTC_2882|Compound:9058044|FD-Score:3.05|P-value:8.71E-4 ID:SGTC_2973|Compound:9094665|FD-Score:4.71|P-value:6.85E-7 ID:SGTC_2981|Compound:9013952|FD-Score:5.82|P-value:1.26E-9 ID:SGTC_2983|Compound:9022671|FD-Score:6.16|P-value:1.39E-10 ID:SGTC_2988|Compound:9073640|FD-Score:-3.29|P-value:3.86E-4 ID:SGTC_3077|Compound:9117319|FD-Score:3.09|P-value:7.54E-4 ID:SGTC_3086|Compound:9117350|FD-Score:3.46|P-value:1.96E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:6.90|P-value:7.45E-13 ID:SGTC_76|Compound:0433-0009|FD-Score:4.21|P-value:7.87E-6 ID:SGTC_143|Compound:4032-1194|FD-Score:-3.34|P-value:3.17E-4 ID:SGTC_154|Compound:3389-1638|FD-Score:5.01|P-value:1.42E-7 ID:SGTC_176|Compound:1486-1328|FD-Score:4.71|P-value:6.79E-7 ID:SGTC_322|Compound:k048-0136|FD-Score:6.57|P-value:8.53E-12 ID:SGTC_324|Compound:1315-0088|FD-Score:3.28|P-value:3.83E-4 ID:SGTC_456|Compound:gingerol|FD-Score:3.75|P-value:6.04E-5 ID:SGTC_523|Compound:1934-0222|FD-Score:3.14|P-value:6.35E-4 ID:SGTC_536|Compound:3852-0578|FD-Score:3.94|P-value:2.63E-5 ID:SGTC_537|Compound:1334-0398|FD-Score:3.84|P-value:4.09E-5 ID:SGTC_655|Compound:k060-0012|FD-Score:3.18|P-value:5.53E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:3.42|P-value:2.26E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:3.49|P-value:1.74E-4 ID:SGTC_927|Compound:2425-1185|FD-Score:3.31|P-value:3.42E-4 ID:SGTC_938|Compound:3126-1755|FD-Score:3.57|P-value:1.26E-4 ID:SGTC_1000|Compound:4048-0884|FD-Score:3.89|P-value:3.37E-5 ID:SGTC_1252|Compound:0493-0875|FD-Score:3.12|P-value:6.86E-4 ID:SGTC_1257|Compound:0669-0121|FD-Score:3.26|P-value:4.20E-4 ID:SGTC_1283|Compound:0922-0125|FD-Score:3.48|P-value:1.79E-4 ID:SGTC_1337|Compound:1486-1011|FD-Score:3.47|P-value:1.88E-4 ID:SGTC_1451|Compound:4152-0210|FD-Score:4.14|P-value:1.08E-5 ID:SGTC_1458|Compound:k015-0034|FD-Score:4.31|P-value:5.00E-6 ID:SGTC_1540|Compound:guanosine|FD-Score:3.89|P-value:3.29E-5 ID:SGTC_1851|Compound:st056236|FD-Score:3.50|P-value:1.63E-4 ID:SGTC_2295|Compound:7971645|FD-Score:-3.73|P-value:6.73E-5 ID:SGTC_2340|Compound:7987427|FD-Score:-5.16|P-value:6.52E-8 ID:SGTC_2351|Compound:9026858|FD-Score:7.16|P-value:1.06E-13 ID:SGTC_2387|Compound:9077560|FD-Score:-3.74|P-value:6.47E-5 ID:SGTC_2393|Compound:9031399|FD-Score:4.29|P-value:5.36E-6 ID:SGTC_2395|Compound:5646831|FD-Score:5.99|P-value:4.14E-10 ID:SGTC_2400|Compound:5203494|FD-Score:4.15|P-value:1.05E-5 ID:SGTC_2405|Compound:ticlatone|FD-Score:4.28|P-value:5.64E-6 ID:SGTC_2642|Compound:cholecalciferol|FD-Score:3.15|P-value:6.26E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:3.65|P-value:9.04E-5 ID:SGTC_2777|Compound:climbazole|FD-Score:3.81|P-value:4.72E-5 ID:SGTC_2788|Compound:5282734|FD-Score:3.39|P-value:2.49E-4 ID:SGTC_2798|Compound:7813637|FD-Score:3.93|P-value:2.83E-5 ID:SGTC_2882|Compound:9058044|FD-Score:3.05|P-value:8.71E-4 ID:SGTC_2973|Compound:9094665|FD-Score:4.71|P-value:6.85E-7 ID:SGTC_2981|Compound:9013952|FD-Score:5.82|P-value:1.26E-9 ID:SGTC_2983|Compound:9022671|FD-Score:6.16|P-value:1.39E-10 ID:SGTC_2988|Compound:9073640|FD-Score:-3.29|P-value:3.86E-4 ID:SGTC_3077|Compound:9117319|FD-Score:3.09|P-value:7.54E-4 ID:SGTC_3086|Compound:9117350|FD-Score:3.46|P-value:1.96E-4

Top fitness defect scores for YNR060W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2351 9026858 7.16 72.3 µM 1.06E-13 RNA processing & uracil transport 3 3.96E-13
2 SGTC_58 0957-0393 6.90 9.0 µM 7.45E-13 cell wall 168 2.53E-12
3 SGTC_322 k048-0136 6.57 19.3 µM 8.53E-12 RSC & ERG11 1 2.59E-11
4 SGTC_2983 9022671 6.16 13.0 µM 1.39E-10 60S ribosome export 2 3.70E-10
5 SGTC_2395 5646831 5.99 180.2 µM 4.14E-10 6 1.05E-9
6 SGTC_2981 9013952 5.82 39.0 µM 1.26E-9 15 3.03E-9
7 SGTC_154 3389-1638 5.01 511.1 µM 1.42E-7 9 2.75E-7
8 SGTC_176 1486-1328 4.71 72.8 µM 6.79E-7 37 1.23E-6
9 SGTC_2973 9094665 4.71 18.0 µM 6.85E-7 NEO1 20 1.23E-6
10 SGTC_1458 k015-0034 4.31 6.3 µM 5.00E-6 12 8.23E-6
11 SGTC_2393 9031399 4.29 185.9 µM 5.36E-6 RNA processing & uracil transport 19 8.81E-6
12 SGTC_2405 ticlatone 4.28 3.5 µM 5.64E-6 59 9.24E-6
13 SGTC_76 0433-0009 4.21 400.3 µM 7.87E-6 9 1.27E-5
14 SGTC_2400 5203494 4.15 178.7 µM 1.05E-5 RSC complex & mRNA processing 22 1.67E-5
15 SGTC_1451 4152-0210 4.14 104.0 µM 1.08E-5 20 1.73E-5
16 SGTC_536 3852-0578 3.94 10.6 µM 2.63E-5 24 4.01E-5
17 SGTC_2798 7813637 3.93 45.5 µM 2.83E-5 32 4.31E-5
18 SGTC_1540 guanosine 3.89 70.6 µM 3.29E-5 9 4.97E-5
19 SGTC_1000 4048-0884 3.89 8.7 µM 3.37E-5 copper-dependent oxidative stress 33 5.09E-5
20 SGTC_537 1334-0398 3.84 209.0 µM 4.09E-5 31 6.13E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.169 5.40E-23 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.165 8.06E-22 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.154 2.37E-19 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.154 3.35E-19 YBR120C CBP6 hom Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.151 1.27E-18 YIL160C POT1 hom 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
0.146 2.39E-17 YKL115C_d YKL115C_d hom Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1
0.144 4.44E-17 YBR021W FUR4 hom Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation
0.140 4.74E-16 YIL148W RPL40A hom Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.136 2.24E-15 YKL033W-A_p YKL033W-A_p hom Putative protein of unknown function; similar to uncharacterized proteins from other fungi
0.131 2.12E-14 YIL123W SIM1 hom Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p) that may participate in DNA replication, promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated
0.129 8.11E-14 YKL076C_d PSY1_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
0.128 1.28E-13 YOR282W_d YOR282W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
0.127 1.60E-13 YDL189W RBS1 hom Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain
0.126 2.68E-13 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.122 1.50E-12 YOR127W RGA1 hom GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress