Protein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress

Zygosity: Homozygous strain
Profile for YNR061C / YNR061C

Click on Significant Values for Screen Details

Top fitness defect scores for YNR061C deletion by condition

RankScreen IDConditionFD ScoreConc
1 SGTC_1245 0418-0076 4.96 17.2 µM 1.06E-7 ERG2 11 3.49E-7
2 SGTC_522 1273-0010 4.96 11.6 µM 1.09E-7 60S ribosome export 11 3.56E-7
3 SGTC_2628 lathosterol 4.91 100.0 µM 1.41E-7 13 4.52E-7
4 SGTC_815 1582-0027 4.67 36.9 µM 5.32E-7 9 1.53E-6
5 SGTC_2184 6075246 4.52 200.0 µM 1.12E-6 14 3.05E-6
6 SGTC_853 0419-0306 4.49 21.0 µM 1.32E-6 6 3.55E-6
7 SGTC_883 0850-0245 4.48 24.6 µM 1.41E-6 cell wall 23 3.76E-6
8 SGTC_1136 pergolide 4.44 1.1 µM 1.70E-6 27 4.47E-6
9 SGTC_2718 lomofungin 4.21 53.0 µM 5.40E-6 mitochondrial processes 6 1.30E-5
10 SGTC_1454 4239-0054 4.14 42.6 µM 7.39E-6 28 1.73E-5
11 SGTC_797 k781-0471 4.13 270.0 µM 7.87E-6 RPP1 & pyrimidine depletion 17 1.84E-5
12 SGTC_2255 7941087 4.06 197.4 µM 1.06E-5 28 2.41E-5
13 SGTC_2561 acetosyringone 3.95 100.0 µM 1.83E-5 25 3.99E-5
14 SGTC_1731 st035964 3.91 63.0 µM 2.12E-5 29 4.57E-5
15 SGTC_408 cantharidin 3.84 100.0 µM 2.93E-5 cell wall signaling 25 6.17E-5
16 SGTC_444 curcumin 3.79 90.4 µM 3.68E-5 iron homeostasis 32 7.61E-5
17 SGTC_98 0074-0023 3.78 107.7 µM 3.85E-5 Golgi 44 7.94E-5
18 SGTC_684 1140-0066 3.73 328.0 µM 4.74E-5 Golgi 30 9.62E-5
19 SGTC_998 3996-0085 3.67 177.0 µM 6.15E-5 30 1.22E-4
20 SGTC_2748 diazoxide 3.63 86.7 µM 7.08E-5 12 1.39E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.176 8.35E-25 YGL090W LIF1 hom Component of the DNA ligase IV complex that mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein
0.144 4.89E-17 YLR040C_p YLR040C_p hom Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential
0.143 8.62E-17 YPL246C RBD2 hom Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p
0.137 1.64E-15 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.135 4.46E-15 YHL045W_d YHL045W_d hom Putative protein of unknown function; not an essential gene
0.134 7.85E-15 YDL155W CLB3 hom B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress
0.130 4.79E-14 YOR271C_p FSF1_p hom Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis
0.119 5.10E-12 YDL022W GPD1 hom NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity
0.117 1.02E-11 YNL123W NMA111 hom Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases
0.112 7.37E-11 YIL009W FAA3 hom Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.108 3.35E-10 YML029W USA1 hom Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p
0.108 4.48E-10 YDR242W AMD2 hom Putative amidase
0.107 4.76E-10 YML025C YML6 het Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins
0.106 6.38E-10 YGR243W FMP43 hom Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium; expression regulated by osmotic and alkaline stresses; protein abundance increases in response to DNA replication stress
0.106 6.46E-10 YOR033C EXO1 hom 5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress