YOL003C / PFA4

Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL003C / PFA4

Click on Significant Values for Screen Details ID:SGTC_15|Compound:0266-0086|FD-Score:3.69|P-value:1.24E-4 ID:SGTC_35|Compound:0133-0054|FD-Score:3.88|P-value:5.83E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:12.10|P-value:2.21E-33 ID:SGTC_63|Compound:0099-0242|FD-Score:-3.24|P-value:6.61E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:4.46|P-value:4.96E-6 ID:SGTC_138|Compound:0850-0259|FD-Score:9.56|P-value:1.24E-21 ID:SGTC_160|Compound:3453-0671|FD-Score:-4.34|P-value:8.29E-6 ID:SGTC_163|Compound:1327-0036|FD-Score:4.37|P-value:7.20E-6 ID:SGTC_192|Compound:3455-0609|FD-Score:-3.18|P-value:8.00E-4 ID:SGTC_206|Compound:4546-0025|FD-Score:4.82|P-value:8.65E-7 ID:SGTC_304|Compound:1310-0298|FD-Score:4.38|P-value:7.09E-6 ID:SGTC_351|Compound:caspofungin|FD-Score:6.86|P-value:4.98E-12 ID:SGTC_354|Compound:2783-3500|FD-Score:3.15|P-value:8.87E-4 ID:SGTC_360|Compound:0957-0399|FD-Score:3.22|P-value:7.13E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:18.10|P-value:3.99E-72 ID:SGTC_586|Compound:0957-0265|FD-Score:6.72|P-value:1.33E-11 ID:SGTC_627|Compound:1391-0720|FD-Score:-3.23|P-value:6.72E-4 ID:SGTC_670|Compound:4204-0025|FD-Score:3.22|P-value:7.07E-4 ID:SGTC_675|Compound:1309-0189|FD-Score:4.42|P-value:5.75E-6 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.13|P-value:2.13E-5 ID:SGTC_839|Compound:0122-0028|FD-Score:3.23|P-value:6.71E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:7.85|P-value:3.36E-15 ID:SGTC_1282|Compound:0886-0010|FD-Score:4.21|P-value:1.50E-5 ID:SGTC_1333|Compound:1482-0047|FD-Score:-3.15|P-value:9.06E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:4.54|P-value:3.34E-6 ID:SGTC_1364|Compound:1653-0028|FD-Score:5.55|P-value:1.86E-8 ID:SGTC_1464|Compound:k208-0027|FD-Score:3.87|P-value:6.17E-5 ID:SGTC_1621|Compound:st003705|FD-Score:3.21|P-value:7.23E-4 ID:SGTC_1733|Compound:st037357|FD-Score:3.14|P-value:9.18E-4 ID:SGTC_1754|Compound:st042065|FD-Score:5.01|P-value:3.37E-7 ID:SGTC_1760|Compound:st045453|FD-Score:5.39|P-value:4.51E-8 ID:SGTC_1800|Compound:st050079|FD-Score:3.93|P-value:4.86E-5 ID:SGTC_1803|Compound:st050068|FD-Score:4.33|P-value:8.58E-6 ID:SGTC_2316|Compound:5844804|FD-Score:-3.84|P-value:6.95E-5 ID:SGTC_2426|Compound:5582046|FD-Score:3.87|P-value:6.26E-5 ID:SGTC_2581|Compound:avocatin a|FD-Score:3.21|P-value:7.32E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:12.10|P-value:1.42E-33 ID:SGTC_2584|Compound:androsterone|FD-Score:11.70|P-value:2.25E-31 ID:SGTC_2619|Compound:obliquin|FD-Score:4.78|P-value:1.04E-6 ID:SGTC_2805|Compound:7950783|FD-Score:3.61|P-value:1.74E-4 ID:SGTC_2969|Compound:9091408|FD-Score:3.24|P-value:6.61E-4 ID:SGTC_2974|Compound:9094440|FD-Score:3.25|P-value:6.34E-4 ID:SGTC_3297|Compound:9117502|FD-Score:-3.23|P-value:6.77E-4 ID:SGTC_15|Compound:0266-0086|FD-Score:3.69|P-value:1.24E-4 ID:SGTC_35|Compound:0133-0054|FD-Score:3.88|P-value:5.83E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:12.10|P-value:2.21E-33 ID:SGTC_63|Compound:0099-0242|FD-Score:-3.24|P-value:6.61E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:4.46|P-value:4.96E-6 ID:SGTC_138|Compound:0850-0259|FD-Score:9.56|P-value:1.24E-21 ID:SGTC_160|Compound:3453-0671|FD-Score:-4.34|P-value:8.29E-6 ID:SGTC_163|Compound:1327-0036|FD-Score:4.37|P-value:7.20E-6 ID:SGTC_192|Compound:3455-0609|FD-Score:-3.18|P-value:8.00E-4 ID:SGTC_206|Compound:4546-0025|FD-Score:4.82|P-value:8.65E-7 ID:SGTC_304|Compound:1310-0298|FD-Score:4.38|P-value:7.09E-6 ID:SGTC_351|Compound:caspofungin|FD-Score:6.86|P-value:4.98E-12 ID:SGTC_354|Compound:2783-3500|FD-Score:3.15|P-value:8.87E-4 ID:SGTC_360|Compound:0957-0399|FD-Score:3.22|P-value:7.13E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:18.10|P-value:3.99E-72 ID:SGTC_586|Compound:0957-0265|FD-Score:6.72|P-value:1.33E-11 ID:SGTC_627|Compound:1391-0720|FD-Score:-3.23|P-value:6.72E-4 ID:SGTC_670|Compound:4204-0025|FD-Score:3.22|P-value:7.07E-4 ID:SGTC_675|Compound:1309-0189|FD-Score:4.42|P-value:5.75E-6 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.13|P-value:2.13E-5 ID:SGTC_839|Compound:0122-0028|FD-Score:3.23|P-value:6.71E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:7.85|P-value:3.36E-15 ID:SGTC_1282|Compound:0886-0010|FD-Score:4.21|P-value:1.50E-5 ID:SGTC_1333|Compound:1482-0047|FD-Score:-3.15|P-value:9.06E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:4.54|P-value:3.34E-6 ID:SGTC_1364|Compound:1653-0028|FD-Score:5.55|P-value:1.86E-8 ID:SGTC_1464|Compound:k208-0027|FD-Score:3.87|P-value:6.17E-5 ID:SGTC_1621|Compound:st003705|FD-Score:3.21|P-value:7.23E-4 ID:SGTC_1733|Compound:st037357|FD-Score:3.14|P-value:9.18E-4 ID:SGTC_1754|Compound:st042065|FD-Score:5.01|P-value:3.37E-7 ID:SGTC_1760|Compound:st045453|FD-Score:5.39|P-value:4.51E-8 ID:SGTC_1800|Compound:st050079|FD-Score:3.93|P-value:4.86E-5 ID:SGTC_1803|Compound:st050068|FD-Score:4.33|P-value:8.58E-6 ID:SGTC_2316|Compound:5844804|FD-Score:-3.84|P-value:6.95E-5 ID:SGTC_2426|Compound:5582046|FD-Score:3.87|P-value:6.26E-5 ID:SGTC_2581|Compound:avocatin a|FD-Score:3.21|P-value:7.32E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:12.10|P-value:1.42E-33 ID:SGTC_2584|Compound:androsterone|FD-Score:11.70|P-value:2.25E-31 ID:SGTC_2619|Compound:obliquin|FD-Score:4.78|P-value:1.04E-6 ID:SGTC_2805|Compound:7950783|FD-Score:3.61|P-value:1.74E-4 ID:SGTC_2969|Compound:9091408|FD-Score:3.24|P-value:6.61E-4 ID:SGTC_2974|Compound:9094440|FD-Score:3.25|P-value:6.34E-4 ID:SGTC_3297|Compound:9117502|FD-Score:-3.23|P-value:6.77E-4

Top fitness defect scores for YOL003C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_514 4466-0038 18.10 2.6 nM 3.99E-72 cell wall 3 2.70E-73
2 SGTC_2582 agaric acid 12.10 100.0 µM 1.42E-33 redox potentiating 40 4.24E-34
3 SGTC_58 0957-0393 12.10 9.0 µM 2.21E-33 cell wall 52 6.64E-34
4 SGTC_2584 androsterone 11.70 48.7 µM 2.25E-31 cell wall 12 7.30E-32
5 SGTC_138 0850-0259 9.56 40.9 µM 1.24E-21 cell wall 47 5.82E-22
6 SGTC_951 1171-0579 7.85 82.7 µM 3.36E-15 cell wall 17 2.02E-15
7 SGTC_351 caspofungin 6.86 25.0 nM 4.98E-12 cell wall 29 3.36E-12
8 SGTC_586 0957-0265 6.72 8.2 µM 1.33E-11 cell wall 13 9.16E-12
9 SGTC_1364 1653-0028 5.55 37.6 µM 1.86E-8 5 1.44E-8
10 SGTC_1760 st045453 5.39 10.4 µM 4.51E-8 NEO1-PIK1 98 3.54E-8
11 SGTC_1754 st042065 5.01 16.1 µM 3.37E-7 NEO1-PIK1 126 2.73E-7
12 SGTC_206 4546-0025 4.82 72.0 µM 8.65E-7 NEO1 44 7.11E-7
13 SGTC_2619 obliquin 4.78 87.9 µM 1.04E-6 cell wall 103 8.55E-7
14 SGTC_1360 1598-0022 4.54 39.8 µM 3.34E-6 ERG2 19 2.81E-6
15 SGTC_99 0109-0120 4.46 622.8 µM 4.96E-6 8 4.19E-6
16 SGTC_675 1309-0189 4.42 238.0 µM 5.75E-6 9 4.87E-6
17 SGTC_304 1310-0298 4.38 97.0 µM 7.09E-6 cell wall 49 6.03E-6
18 SGTC_163 1327-0036 4.37 7.5 µM 7.20E-6 87 6.13E-6
19 SGTC_1803 st050068 4.33 52.4 µM 8.58E-6 NEO1-PIK1 152 7.32E-6
20 SGTC_1282 0886-0010 4.21 32.4 µM 1.50E-5 endomembrane recycling 29 1.29E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.514 1.15E-225 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.495 2.49E-207 YBR134W_d YBR134W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.443 1.86E-161 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.442 1.01E-160 YBR020W GAL1 hom Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication
0.436 6.86E-156 YJL099W CHS6 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.436 1.00E-155 YBR023C CHS3 hom Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
0.432 1.68E-152 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.422 2.99E-145 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.405 2.73E-132 YMR121C RPL15B hom Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress
0.399 2.08E-128 YPL269W KAR9 hom Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase
0.398 5.39E-128 YOR051C ETT1 hom Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough
0.395 6.71E-126 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.391 5.54E-123 YDR335W MSN5 hom Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm
0.391 8.91E-123 YCL005W LDB16 hom Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria
0.391 1.15E-122 YGL033W HOP2 hom Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair