YOL005C / RPB11

RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOL005C / RPB11

Click on Significant Values for Screen Details ID:SGTC_121|Compound:0557-0495|FD-Score:3.27|P-value:7.26E-4 ID:SGTC_174|Compound:3553-0389|FD-Score:3.24|P-value:8.04E-4 ID:SGTC_227|Compound:fluconazole|FD-Score:3.18|P-value:9.76E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:4.89|P-value:1.05E-6 ID:SGTC_344|Compound:k064-0035|FD-Score:-3.98|P-value:6.62E-5 ID:SGTC_346|Compound:4513-0042|FD-Score:3.21|P-value:9.01E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:4.65|P-value:3.23E-6 ID:SGTC_354|Compound:2783-3500|FD-Score:3.44|P-value:4.05E-4 ID:SGTC_518|Compound:1021-0119|FD-Score:3.61|P-value:2.27E-4 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:4.06|P-value:4.02E-5 ID:SGTC_644|Compound:1498-1197|FD-Score:-3.29|P-value:7.84E-4 ID:SGTC_655|Compound:k060-0012|FD-Score:3.41|P-value:4.53E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:4.17|P-value:2.51E-5 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.81|P-value:1.52E-6 ID:SGTC_709|Compound:3807-1509|FD-Score:3.41|P-value:4.52E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:3.54|P-value:2.91E-4 ID:SGTC_839|Compound:0122-0028|FD-Score:4.51|P-value:5.91E-6 ID:SGTC_928|Compound:2764-0428|FD-Score:3.52|P-value:3.08E-4 ID:SGTC_1024|Compound:4507-0395|FD-Score:5.45|P-value:6.25E-8 ID:SGTC_1050|Compound:3232-1156|FD-Score:3.79|P-value:1.16E-4 ID:SGTC_1069|Compound:diphenylcyclopropenone|FD-Score:5.69|P-value:1.68E-8 ID:SGTC_1081|Compound:sertraline|FD-Score:-3.39|P-value:5.65E-4 ID:SGTC_1229|Compound:0139-0193|FD-Score:9.52|P-value:1.43E-20 ID:SGTC_1233|Compound:0325-0408|FD-Score:3.62|P-value:2.17E-4 ID:SGTC_1234|Compound:0326-0494|FD-Score:5.60|P-value:2.80E-8 ID:SGTC_1342|Compound:1486-1464|FD-Score:4.75|P-value:1.96E-6 ID:SGTC_1374|Compound:2889-5571|FD-Score:6.04|P-value:2.32E-9 ID:SGTC_1570|Compound:verbenone|FD-Score:-3.37|P-value:6.11E-4 ID:SGTC_1652|Compound:st011932|FD-Score:8.38|P-value:2.31E-16 ID:SGTC_1677|Compound:st019204|FD-Score:5.82|P-value:8.07E-9 ID:SGTC_1774|Compound:st048371|FD-Score:4.45|P-value:7.74E-6 ID:SGTC_1784|Compound:st039334|FD-Score:3.98|P-value:5.41E-5 ID:SGTC_1896|Compound:st058441|FD-Score:3.24|P-value:8.21E-4 ID:SGTC_1915|Compound:st067105|FD-Score:5.07|P-value:4.33E-7 ID:SGTC_1925|Compound:st057770|FD-Score:4.27|P-value:1.68E-5 ID:SGTC_1949|Compound:st076024|FD-Score:-3.75|P-value:1.58E-4 ID:SGTC_1954|Compound:st077032|FD-Score:-3.49|P-value:4.05E-4 ID:SGTC_1964|Compound:st076914|FD-Score:3.31|P-value:6.31E-4 ID:SGTC_2041|Compound:5107442|FD-Score:6.92|P-value:9.98E-12 ID:SGTC_2109|Compound:5483026|FD-Score:4.95|P-value:7.91E-7 ID:SGTC_2124|Compound:5246320|FD-Score:3.66|P-value:1.89E-4 ID:SGTC_2163|Compound:5671233|FD-Score:4.09|P-value:3.48E-5 ID:SGTC_2187|Compound:6125751|FD-Score:5.54|P-value:3.88E-8 ID:SGTC_2348|Compound:9022543|FD-Score:3.85|P-value:9.21E-5 ID:SGTC_2357|Compound:9067248|FD-Score:3.52|P-value:3.10E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:4.94|P-value:8.03E-7 ID:SGTC_2534|Compound:helenine|FD-Score:3.79|P-value:1.17E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:4.69|P-value:2.60E-6 ID:SGTC_2575|Compound:plumbagin|FD-Score:6.09|P-value:1.75E-9 ID:SGTC_2782|Compound:7736369|FD-Score:-3.22|P-value:9.86E-4 ID:SGTC_2789|Compound:5281112|FD-Score:4.21|P-value:2.17E-5 ID:SGTC_2840|Compound:9008771|FD-Score:-3.80|P-value:1.29E-4 ID:SGTC_2930|Compound:9013230|FD-Score:4.79|P-value:1.68E-6 ID:SGTC_3343|Compound:9148379|FD-Score:-3.24|P-value:9.43E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:3.27|P-value:7.26E-4 ID:SGTC_174|Compound:3553-0389|FD-Score:3.24|P-value:8.04E-4 ID:SGTC_227|Compound:fluconazole|FD-Score:3.18|P-value:9.76E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:4.89|P-value:1.05E-6 ID:SGTC_344|Compound:k064-0035|FD-Score:-3.98|P-value:6.62E-5 ID:SGTC_346|Compound:4513-0042|FD-Score:3.21|P-value:9.01E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:4.65|P-value:3.23E-6 ID:SGTC_354|Compound:2783-3500|FD-Score:3.44|P-value:4.05E-4 ID:SGTC_518|Compound:1021-0119|FD-Score:3.61|P-value:2.27E-4 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:4.06|P-value:4.02E-5 ID:SGTC_644|Compound:1498-1197|FD-Score:-3.29|P-value:7.84E-4 ID:SGTC_655|Compound:k060-0012|FD-Score:3.41|P-value:4.53E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:4.17|P-value:2.51E-5 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.81|P-value:1.52E-6 ID:SGTC_709|Compound:3807-1509|FD-Score:3.41|P-value:4.52E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:3.54|P-value:2.91E-4 ID:SGTC_839|Compound:0122-0028|FD-Score:4.51|P-value:5.91E-6 ID:SGTC_928|Compound:2764-0428|FD-Score:3.52|P-value:3.08E-4 ID:SGTC_1024|Compound:4507-0395|FD-Score:5.45|P-value:6.25E-8 ID:SGTC_1050|Compound:3232-1156|FD-Score:3.79|P-value:1.16E-4 ID:SGTC_1069|Compound:diphenylcyclopropenone|FD-Score:5.69|P-value:1.68E-8 ID:SGTC_1081|Compound:sertraline|FD-Score:-3.39|P-value:5.65E-4 ID:SGTC_1229|Compound:0139-0193|FD-Score:9.52|P-value:1.43E-20 ID:SGTC_1233|Compound:0325-0408|FD-Score:3.62|P-value:2.17E-4 ID:SGTC_1234|Compound:0326-0494|FD-Score:5.60|P-value:2.80E-8 ID:SGTC_1342|Compound:1486-1464|FD-Score:4.75|P-value:1.96E-6 ID:SGTC_1374|Compound:2889-5571|FD-Score:6.04|P-value:2.32E-9 ID:SGTC_1570|Compound:verbenone|FD-Score:-3.37|P-value:6.11E-4 ID:SGTC_1652|Compound:st011932|FD-Score:8.38|P-value:2.31E-16 ID:SGTC_1677|Compound:st019204|FD-Score:5.82|P-value:8.07E-9 ID:SGTC_1774|Compound:st048371|FD-Score:4.45|P-value:7.74E-6 ID:SGTC_1784|Compound:st039334|FD-Score:3.98|P-value:5.41E-5 ID:SGTC_1896|Compound:st058441|FD-Score:3.24|P-value:8.21E-4 ID:SGTC_1915|Compound:st067105|FD-Score:5.07|P-value:4.33E-7 ID:SGTC_1925|Compound:st057770|FD-Score:4.27|P-value:1.68E-5 ID:SGTC_1949|Compound:st076024|FD-Score:-3.75|P-value:1.58E-4 ID:SGTC_1954|Compound:st077032|FD-Score:-3.49|P-value:4.05E-4 ID:SGTC_1964|Compound:st076914|FD-Score:3.31|P-value:6.31E-4 ID:SGTC_2041|Compound:5107442|FD-Score:6.92|P-value:9.98E-12 ID:SGTC_2109|Compound:5483026|FD-Score:4.95|P-value:7.91E-7 ID:SGTC_2124|Compound:5246320|FD-Score:3.66|P-value:1.89E-4 ID:SGTC_2163|Compound:5671233|FD-Score:4.09|P-value:3.48E-5 ID:SGTC_2187|Compound:6125751|FD-Score:5.54|P-value:3.88E-8 ID:SGTC_2348|Compound:9022543|FD-Score:3.85|P-value:9.21E-5 ID:SGTC_2357|Compound:9067248|FD-Score:3.52|P-value:3.10E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:4.94|P-value:8.03E-7 ID:SGTC_2534|Compound:helenine|FD-Score:3.79|P-value:1.17E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:4.69|P-value:2.60E-6 ID:SGTC_2575|Compound:plumbagin|FD-Score:6.09|P-value:1.75E-9 ID:SGTC_2782|Compound:7736369|FD-Score:-3.22|P-value:9.86E-4 ID:SGTC_2789|Compound:5281112|FD-Score:4.21|P-value:2.17E-5 ID:SGTC_2840|Compound:9008771|FD-Score:-3.80|P-value:1.29E-4 ID:SGTC_2930|Compound:9013230|FD-Score:4.79|P-value:1.68E-6 ID:SGTC_3343|Compound:9148379|FD-Score:-3.24|P-value:9.43E-4

Top fitness defect scores for YOL005C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1229 0139-0193 9.52 12.2 µM 1.43E-20 RSC complex & mRNA processing 3 8.33E-22 2.12
2 SGTC_1652 st011932 8.38 78.0 µM 2.31E-16 RSC complex & mRNA processing 15 2.60E-17 0.78
3 SGTC_2041 5107442 6.92 45.3 µM 9.98E-12 RSC complex & mRNA processing 13 2.29E-12 0.02
4 SGTC_2575 plumbagin 6.09 510.0 nM 1.75E-9 superoxide 12 5.63E-10 0.25
5 SGTC_1374 2889-5571 6.04 141.0 µM 2.32E-9 RSC complex & mRNA processing 20 7.62E-10 0.07
6 SGTC_1677 st019204 5.82 53.9 µM 8.07E-9 2 2.87E-9 1.17
7 SGTC_1069 diphenylcyclopropenone 5.69 8.5 µM 1.68E-8 4 6.27E-9 1.17
8 SGTC_1234 0326-0494 5.60 15.5 µM 2.80E-8 1 1.08E-8 0.98
9 SGTC_2187 6125751 5.54 200.0 µM 3.88E-8 RSC complex & mRNA processing 4 1.53E-8 0.01
10 SGTC_1024 4507-0395 5.45 26.0 µM 6.25E-8 TSC3-RPN4 2 2.54E-8 0.52
11 SGTC_1915 st067105 5.07 30.6 µM 4.33E-7 RNA pol III & RNase P/MRP 27 2.00E-7 0.61
12 SGTC_2109 5483026 4.95 200.0 µM 7.91E-7 RSC complex & mRNA processing 25 3.79E-7 0.09
13 SGTC_2408 sulbentine 4.94 18.4 µM 8.03E-7 23 3.85E-7 0.18
14 SGTC_236 1509-0011 4.89 5.3 µM 1.05E-6 16 5.12E-7 0.09
15 SGTC_682 0kpi-0099 4.81 17.0 µM 1.52E-6 20 7.58E-7 0.11
16 SGTC_2930 9013230 4.79 14.9 µM 1.68E-6 heme biosynthesis & mitochondrial translocase 12 8.42E-7 0.28
17 SGTC_1342 1486-1464 4.75 234.0 µM 1.96E-6 1 9.95E-7 0.88
18 SGTC_2544 sphondin 4.69 68.5 µM 2.60E-6 RNA pol III & RNase P/MRP 27 1.34E-6 0.07
19 SGTC_351 caspofungin 4.65 25.0 nM 3.23E-6 cell wall 22 1.69E-6 0.00
20 SGTC_839 0122-0028 4.51 230.0 µM 5.91E-6 RSC complex & mRNA processing 28 3.21E-6 0.05

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.404 3.17E-132 YER165W PAB1 het Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G
0.300 1.50E-70 YPR104C FHL1 het Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants
0.280 1.58E-61 YNR016C ACC1 het Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
0.278 1.16E-60 YML127W RSC9 het Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
0.264 8.99E-55 YFR037C RSC8 het Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
0.262 7.47E-54 YDR404C RPB7 het RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
0.253 5.65E-50 YJL081C ARP4 het Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
0.252 9.53E-50 YLR321C SFH1 het Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog
0.239 6.43E-45 YPR034W ARP7 het Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
0.239 1.01E-44 YBR091C TIM12 het Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins
0.238 1.60E-44 YBL092W RPL32 het Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog
0.237 3.45E-44 YFL002C SPB4 het Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients
0.233 1.78E-42 YBL026W LSM2 het Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
0.232 4.41E-42 YLR033W RSC58 het Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
0.227 1.70E-40 YOR236W DFR1 het Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism