YOL006C / TOP1

Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL006C / TOP1

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:-4.17|P-value:6.63E-5 ID:SGTC_202|Compound:3594-0513|FD-Score:6.52|P-value:5.00E-10 ID:SGTC_268|Compound:camptothecin|FD-Score:-5.90|P-value:2.89E-8 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.57|P-value:5.54E-4 ID:SGTC_537|Compound:1334-0398|FD-Score:4.38|P-value:1.93E-5 ID:SGTC_736|Compound:0958-0254|FD-Score:3.87|P-value:1.31E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:7.51|P-value:1.08E-12 ID:SGTC_853|Compound:0419-0306|FD-Score:3.96|P-value:9.61E-5 ID:SGTC_885|Compound:0865-0106|FD-Score:-4.30|P-value:4.10E-5 ID:SGTC_890|Compound:0929-0006|FD-Score:-3.60|P-value:5.06E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:-4.07|P-value:9.74E-5 ID:SGTC_940|Compound:3232-1287|FD-Score:-3.55|P-value:5.79E-4 ID:SGTC_972|Compound:1319-0219|FD-Score:3.59|P-value:3.52E-4 ID:SGTC_983|Compound:1491-0821|FD-Score:4.98|P-value:1.43E-6 ID:SGTC_1050|Compound:3232-1156|FD-Score:3.56|P-value:3.96E-4 ID:SGTC_1212|Compound:0520-0165|FD-Score:3.38|P-value:7.18E-4 ID:SGTC_1345|Compound:1488-0192|FD-Score:5.44|P-value:1.65E-7 ID:SGTC_1346|Compound:1488-2224|FD-Score:-3.45|P-value:8.01E-4 ID:SGTC_1827|Compound:berberine|FD-Score:-4.33|P-value:3.54E-5 ID:SGTC_1836|Compound:st055642|FD-Score:5.33|P-value:2.85E-7 ID:SGTC_1838|Compound:st055369|FD-Score:5.99|P-value:9.97E-9 ID:SGTC_1842|Compound:st055362|FD-Score:4.32|P-value:2.44E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:-4.97|P-value:2.51E-6 ID:SGTC_1901|Compound:st060216|FD-Score:3.72|P-value:2.28E-4 ID:SGTC_1915|Compound:st067105|FD-Score:5.39|P-value:2.15E-7 ID:SGTC_1930|Compound:st074634|FD-Score:6.14|P-value:4.37E-9 ID:SGTC_2128|Compound:5255449|FD-Score:7.17|P-value:9.43E-12 ID:SGTC_2142|Compound:5622915|FD-Score:5.65|P-value:5.67E-8 ID:SGTC_2260|Compound:7964312|FD-Score:3.31|P-value:8.83E-4 ID:SGTC_2338|Compound:7999974|FD-Score:-3.74|P-value:3.15E-4 ID:SGTC_2347|Compound:9049783|FD-Score:3.30|P-value:9.20E-4 ID:SGTC_2402|Compound:5553605|FD-Score:-3.51|P-value:6.64E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:4.00|P-value:8.31E-5 ID:SGTC_2467|Compound:5474540|FD-Score:3.46|P-value:5.38E-4 ID:SGTC_2532|Compound:xanthone|FD-Score:5.24|P-value:4.34E-7 ID:SGTC_2622|Compound:2-methoxyxanthone|FD-Score:3.43|P-value:6.03E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.13|P-value:5.03E-5 ID:SGTC_2760|Compound:etidronate|FD-Score:5.72|P-value:4.02E-8 ID:SGTC_2854|Compound:9024559|FD-Score:3.56|P-value:3.95E-4 ID:SGTC_2937|Compound:9044753|FD-Score:4.09|P-value:5.80E-5 ID:SGTC_3152|Compound:9099253|FD-Score:-3.77|P-value:2.85E-4 ID:SGTC_3159|Compound:9100096|FD-Score:3.69|P-value:2.48E-4 ID:SGTC_3247|Compound:9135149|FD-Score:7.28|P-value:4.80E-12 ID:SGTC_3325|Compound:9138760|FD-Score:3.73|P-value:2.19E-4 ID:SGTC_3345|Compound:9148410|FD-Score:-3.66|P-value:4.10E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-4.17|P-value:6.63E-5 ID:SGTC_202|Compound:3594-0513|FD-Score:6.52|P-value:5.00E-10 ID:SGTC_268|Compound:camptothecin|FD-Score:-5.90|P-value:2.89E-8 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.57|P-value:5.54E-4 ID:SGTC_537|Compound:1334-0398|FD-Score:4.38|P-value:1.93E-5 ID:SGTC_736|Compound:0958-0254|FD-Score:3.87|P-value:1.31E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:7.51|P-value:1.08E-12 ID:SGTC_853|Compound:0419-0306|FD-Score:3.96|P-value:9.61E-5 ID:SGTC_885|Compound:0865-0106|FD-Score:-4.30|P-value:4.10E-5 ID:SGTC_890|Compound:0929-0006|FD-Score:-3.60|P-value:5.06E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:-4.07|P-value:9.74E-5 ID:SGTC_940|Compound:3232-1287|FD-Score:-3.55|P-value:5.79E-4 ID:SGTC_972|Compound:1319-0219|FD-Score:3.59|P-value:3.52E-4 ID:SGTC_983|Compound:1491-0821|FD-Score:4.98|P-value:1.43E-6 ID:SGTC_1050|Compound:3232-1156|FD-Score:3.56|P-value:3.96E-4 ID:SGTC_1212|Compound:0520-0165|FD-Score:3.38|P-value:7.18E-4 ID:SGTC_1345|Compound:1488-0192|FD-Score:5.44|P-value:1.65E-7 ID:SGTC_1346|Compound:1488-2224|FD-Score:-3.45|P-value:8.01E-4 ID:SGTC_1827|Compound:berberine|FD-Score:-4.33|P-value:3.54E-5 ID:SGTC_1836|Compound:st055642|FD-Score:5.33|P-value:2.85E-7 ID:SGTC_1838|Compound:st055369|FD-Score:5.99|P-value:9.97E-9 ID:SGTC_1842|Compound:st055362|FD-Score:4.32|P-value:2.44E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:-4.97|P-value:2.51E-6 ID:SGTC_1901|Compound:st060216|FD-Score:3.72|P-value:2.28E-4 ID:SGTC_1915|Compound:st067105|FD-Score:5.39|P-value:2.15E-7 ID:SGTC_1930|Compound:st074634|FD-Score:6.14|P-value:4.37E-9 ID:SGTC_2128|Compound:5255449|FD-Score:7.17|P-value:9.43E-12 ID:SGTC_2142|Compound:5622915|FD-Score:5.65|P-value:5.67E-8 ID:SGTC_2260|Compound:7964312|FD-Score:3.31|P-value:8.83E-4 ID:SGTC_2338|Compound:7999974|FD-Score:-3.74|P-value:3.15E-4 ID:SGTC_2347|Compound:9049783|FD-Score:3.30|P-value:9.20E-4 ID:SGTC_2402|Compound:5553605|FD-Score:-3.51|P-value:6.64E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:4.00|P-value:8.31E-5 ID:SGTC_2467|Compound:5474540|FD-Score:3.46|P-value:5.38E-4 ID:SGTC_2532|Compound:xanthone|FD-Score:5.24|P-value:4.34E-7 ID:SGTC_2622|Compound:2-methoxyxanthone|FD-Score:3.43|P-value:6.03E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.13|P-value:5.03E-5 ID:SGTC_2760|Compound:etidronate|FD-Score:5.72|P-value:4.02E-8 ID:SGTC_2854|Compound:9024559|FD-Score:3.56|P-value:3.95E-4 ID:SGTC_2937|Compound:9044753|FD-Score:4.09|P-value:5.80E-5 ID:SGTC_3152|Compound:9099253|FD-Score:-3.77|P-value:2.85E-4 ID:SGTC_3159|Compound:9100096|FD-Score:3.69|P-value:2.48E-4 ID:SGTC_3247|Compound:9135149|FD-Score:7.28|P-value:4.80E-12 ID:SGTC_3325|Compound:9138760|FD-Score:3.73|P-value:2.19E-4 ID:SGTC_3345|Compound:9148410|FD-Score:-3.66|P-value:4.10E-4

Top fitness defect scores for YOL006C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_829 1635-0120 7.51 117.0 µM 1.08E-12 DNA damage response 8 3.04E-14
2 SGTC_3247 9135149 7.28 49.5 µM 4.80E-12 DNA damage response 7 1.69E-13
3 SGTC_2128 5255449 7.17 168.5 µM 9.43E-12 2 3.67E-13
4 SGTC_202 3594-0513 6.52 34.8 µM 5.00E-10 PDR1 2 3.48E-11
5 SGTC_1930 st074634 6.14 34.3 µM 4.37E-9 RNA pol III & RNase P/MRP 9 4.17E-10
6 SGTC_1838 st055369 5.99 84.5 µM 9.97E-9 4 1.07E-9
7 SGTC_2760 etidronate 5.72 98.0 µM 4.02E-8 4 5.29E-9
8 SGTC_2142 5622915 5.65 118.5 µM 5.67E-8 3 7.84E-9
9 SGTC_1345 1488-0192 5.44 31.4 µM 1.65E-7 4 2.66E-8
10 SGTC_1915 st067105 5.39 30.6 µM 2.15E-7 RNA pol III & RNase P/MRP 11 3.61E-8
11 SGTC_1836 st055642 5.33 81.0 µM 2.85E-7 5 4.97E-8
12 SGTC_2532 xanthone 5.24 78.9 µM 4.34E-7 14 8.03E-8
13 SGTC_983 1491-0821 4.98 12.2 µM 1.43E-6 4 3.14E-7
14 SGTC_537 1334-0398 4.38 209.0 µM 1.93E-5 16 6.06E-6
15 SGTC_1842 st055362 4.32 61.2 µM 2.44E-5 7 7.92E-6
16 SGTC_2688 kanamycin b 4.13 96.7 µM 5.03E-5 calcium & mitochondrial duress 343 1.81E-5
17 SGTC_2937 9044753 4.09 11.3 µM 5.80E-5 60S ribosome export 30 2.12E-5
18 SGTC_2406 ethylparaben 4.00 405.7 µM 8.31E-5 35 3.20E-5
19 SGTC_853 0419-0306 3.96 21.0 µM 9.61E-5 12 3.77E-5
20 SGTC_736 0958-0254 3.87 92.6 µM 1.31E-4 Golgi 35 5.37E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.307 5.52E-74 YOR191W ULS1 hom Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.205 3.91E-33 YOR116C RPO31 het RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
0.163 2.15E-21 YDR225W HTA1 hom Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p
0.158 3.03E-20 YDR004W RAD57 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
0.147 1.37E-17 YDR076W RAD55 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
0.143 8.82E-17 YNL248C RPA49 hom RNA polymerase I subunit A49
0.141 2.02E-16 YGL163C RAD54 hom DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress
0.134 7.32E-15 YJL148W RPA34 hom RNA polymerase I subunit A34.5
0.133 1.01E-14 YBR202W MCM7 het Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
0.118 8.48E-12 YBL039C URA7 hom Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication
0.116 1.42E-11 YBR154C RPB5 het RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation
0.115 2.83E-11 YOR207C RET1 het Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
0.112 6.51E-11 YNL108C_p YNL108C_p hom Putative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication
0.112 7.69E-11 YDL150W RPC53 het RNA polymerase III subunit C53
0.104 1.30E-9 YDL136W RPL35B hom Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication