YOL010W / RCL1

Endonuclease that cleaves pre-rRNA at site A2 for 18S rRNA biogenesis; subunit of U3-containing 90S preribosome processome complex involved in small ribosomal subunit assembly; stimulates Bms1p GTPase and U3 binding activity; similar to RNA cyclase-like proteins but no cyclase activity detected

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOL010W / RCL1

Click on Significant Values for Screen Details ID:SGTC_52|Compound:0329-0193|FD-Score:3.02|P-value:9.77E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:3.44|P-value:2.13E-4 ID:SGTC_752|Compound:1896-0090|FD-Score:3.73|P-value:6.80E-5 ID:SGTC_830|Compound:4076-0286|FD-Score:3.89|P-value:3.44E-5 ID:SGTC_1045|Compound:1348-1485|FD-Score:3.65|P-value:9.17E-5 ID:SGTC_1262|Compound:0737-0114|FD-Score:3.06|P-value:8.52E-4 ID:SGTC_1372|Compound:2752-0194|FD-Score:-3.25|P-value:6.31E-4 ID:SGTC_1782|Compound:st039423|FD-Score:3.03|P-value:9.23E-4 ID:SGTC_1846|Compound:st056244|FD-Score:-4.38|P-value:6.41E-6 ID:SGTC_1912|Compound:st060209|FD-Score:3.68|P-value:8.29E-5 ID:SGTC_1913|Compound:st060213|FD-Score:-4.02|P-value:3.19E-5 ID:SGTC_1917|Compound:st066507|FD-Score:-3.64|P-value:1.52E-4 ID:SGTC_2059|Compound:5238311|FD-Score:3.16|P-value:5.84E-4 ID:SGTC_2317|Compound:5986382|FD-Score:4.30|P-value:5.62E-6 ID:SGTC_2333|Compound:9017922|FD-Score:3.34|P-value:3.04E-4 ID:SGTC_2362|Compound:9071156|FD-Score:-3.47|P-value:2.87E-4 ID:SGTC_2407|Compound:paclitaxel|FD-Score:-3.82|P-value:7.44E-5 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.44|P-value:2.11E-4 ID:SGTC_2851|Compound:9015922|FD-Score:-3.24|P-value:6.58E-4 ID:SGTC_3146|Compound:9095609|FD-Score:3.32|P-value:3.34E-4 ID:SGTC_3265|Compound:9137266|FD-Score:-3.77|P-value:9.09E-5 ID:SGTC_52|Compound:0329-0193|FD-Score:3.02|P-value:9.77E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:3.44|P-value:2.13E-4 ID:SGTC_752|Compound:1896-0090|FD-Score:3.73|P-value:6.80E-5 ID:SGTC_830|Compound:4076-0286|FD-Score:3.89|P-value:3.44E-5 ID:SGTC_1045|Compound:1348-1485|FD-Score:3.65|P-value:9.17E-5 ID:SGTC_1262|Compound:0737-0114|FD-Score:3.06|P-value:8.52E-4 ID:SGTC_1372|Compound:2752-0194|FD-Score:-3.25|P-value:6.31E-4 ID:SGTC_1782|Compound:st039423|FD-Score:3.03|P-value:9.23E-4 ID:SGTC_1846|Compound:st056244|FD-Score:-4.38|P-value:6.41E-6 ID:SGTC_1912|Compound:st060209|FD-Score:3.68|P-value:8.29E-5 ID:SGTC_1913|Compound:st060213|FD-Score:-4.02|P-value:3.19E-5 ID:SGTC_1917|Compound:st066507|FD-Score:-3.64|P-value:1.52E-4 ID:SGTC_2059|Compound:5238311|FD-Score:3.16|P-value:5.84E-4 ID:SGTC_2317|Compound:5986382|FD-Score:4.30|P-value:5.62E-6 ID:SGTC_2333|Compound:9017922|FD-Score:3.34|P-value:3.04E-4 ID:SGTC_2362|Compound:9071156|FD-Score:-3.47|P-value:2.87E-4 ID:SGTC_2407|Compound:paclitaxel|FD-Score:-3.82|P-value:7.44E-5 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.44|P-value:2.11E-4 ID:SGTC_2851|Compound:9015922|FD-Score:-3.24|P-value:6.58E-4 ID:SGTC_3146|Compound:9095609|FD-Score:3.32|P-value:3.34E-4 ID:SGTC_3265|Compound:9137266|FD-Score:-3.77|P-value:9.09E-5

Top fitness defect scores for YOL010W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2317 5986382 4.30 200.0 µM 5.62E-6 2 8.54E-6 0.32
2 SGTC_830 4076-0286 3.89 57.8 µM 3.44E-5 translation 5 4.97E-5 0.04
3 SGTC_752 1896-0090 3.73 130.0 µM 6.80E-5 TSC3-RPN4 8 9.63E-5 0.19
4 SGTC_1912 st060209 3.68 90.8 µM 8.29E-5 16 1.17E-4 0.19
5 SGTC_1045 1348-1485 3.65 165.0 µM 9.17E-5 6 1.29E-4 0.24
6 SGTC_2621 lithochol-11-enic acid 3.44 100.0 µM 2.11E-4 13 2.89E-4 0.17
7 SGTC_353 1988-1175 3.44 58.3 µM 2.13E-4 unfolded protein response 13 2.92E-4 0.02
8 SGTC_2333 9017922 3.34 200.0 µM 3.04E-4 8 4.13E-4 0.21
9 SGTC_3146 9095609 3.32 49.5 µM 3.34E-4 9 4.53E-4 0.22
10 SGTC_2059 5238311 3.16 157.0 µM 5.84E-4 9 7.78E-4 0.06
11 SGTC_1262 0737-0114 3.06 49.6 µM 8.52E-4 13 0.00112 0.22
12 SGTC_1782 st039423 3.03 80.6 µM 9.23E-4 9 0.00121 0.00
13 SGTC_52 0329-0193 3.02 875.0 nM 9.77E-4 TSC3-RPN4 15 0.00128 0.04
14 SGTC_1578 josamycin 2.98 24.2 µM 0.00111 15 0.00145 0.20
15 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 2.95 3.7 µM 0.00120 26 0.00156 0.03
16 SGTC_821 1114-0266 2.95 29.2 µM 0.00123 12 0.00160 0.04
17 SGTC_1274 0865-0114 2.94 173.0 µM 0.00127 24 0.00165 0.04
18 SGTC_1172 0370-0043 2.93 85.0 µM 0.00128 15 0.00167 0.05
19 SGTC_306 1672-3436 2.92 11.6 µM 0.00137 Golgi 14 0.00177 0.12
20 SGTC_1920 st075728 2.91 45.7 µM 0.00138 16 0.00179 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.128 1.24E-13 YOL150C_d YOL150C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.120 3.27E-12 YLR187W SKG3 hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication
0.109 2.81E-10 YPL081W RPS9A hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9A has a paralog, RPS9B, that arose from the whole genome duplication
0.101 5.38E-9 YIR043C YIR043C hom Possible pseudogene in strain S288C; YIR043C and the adjacent ORF, YIR044C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family
0.098 1.33E-8 YDR059C UBC5 hom Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication
0.094 5.70E-8 YIR010W DSN1 het Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation
0.092 8.98E-8 YPL192C PRM3 hom Pheromone-regulated protein required for nuclear envelope fusion during karyogamy; localizes to the outer face of the nuclear membrane; interacts with Kar5p at the spindle pole body
0.091 1.46E-7 YNR002C ATO2 hom Putative transmembrane protein involved in export of ammonia; ammonia is a starvation signal that promotes cell death in aging colonies; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family; homolog of Y. lipolytica Gpr1p; ATO2 has a paralog, ADY2, that arose from the whole genome duplication
0.089 2.60E-7 YLR122C_d YLR122C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C
0.088 3.23E-7 YHL026C_p YHL026C_p hom Putative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History)
0.084 1.04E-6 YKL061W_p BLI1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome
0.084 1.09E-6 YAL031C GIP4 hom Cytoplasmic Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate
0.084 1.12E-6 YCL050C APA1 hom AP4A phosphorylase; bifunctional diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase and ADP sulfurylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; catalyzes phosphorolysis of dinucleoside oligophosphates, cleaving substrates' alpha/beta-anhydride bond and introducing Pi into the beta-position of the corresponding NDP formed; protein abundance increases under DNA replication stress; APA1 has a paralog, APA2, that arose from the whole genome duplication
0.081 2.40E-6 YOL024W_p YOL024W_p hom Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site; YOL024W has a paralog, IGD1, that arose from the whole genome duplication
0.081 2.40E-6 YJR107W_p YJR107W_p hom Putative lipase