YOL018C / TLG2

Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16)

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL018C / TLG2

Click on Significant Values for Screen Details ID:SGTC_66|Compound:0109-0187|FD-Score:5.64|P-value:3.55E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:6.72|P-value:2.66E-5 ID:SGTC_169|Compound:k015-0017|FD-Score:-5.30|P-value:5.95E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:7.62|P-value:2.24E-6 ID:SGTC_232|Compound:clozapine|FD-Score:9.35|P-value:8.97E-9 ID:SGTC_301|Compound:4119-0100|FD-Score:5.90|P-value:1.97E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:5.50|P-value:4.81E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:6.81|P-value:2.13E-5 ID:SGTC_389|Compound:tpck|FD-Score:5.35|P-value:6.70E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:6.07|P-value:1.34E-4 ID:SGTC_449|Compound:xestospongin c|FD-Score:7.17|P-value:8.01E-6 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-8.59|P-value:8.25E-8 ID:SGTC_459|Compound:nigericin|FD-Score:11.50|P-value:1.65E-12 ID:SGTC_488|Compound:nitrendipine|FD-Score:5.93|P-value:1.85E-4 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:6.23|P-value:9.09E-5 ID:SGTC_706|Compound:4262-0057|FD-Score:-5.26|P-value:6.45E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:7.56|P-value:2.73E-6 ID:SGTC_949|Compound:1165-0505|FD-Score:6.97|P-value:1.40E-5 ID:SGTC_1010|Compound:4112-3296|FD-Score:6.26|P-value:8.46E-5 ID:SGTC_1026|Compound:k007-1105|FD-Score:10.30|P-value:2.83E-10 ID:SGTC_1060|Compound:3448-7350|FD-Score:5.78|P-value:2.61E-4 ID:SGTC_1069|Compound:diphenylcyclopropenone|FD-Score:6.93|P-value:1.55E-5 ID:SGTC_1078|Compound:idebenone|FD-Score:6.62|P-value:3.46E-5 ID:SGTC_1104|Compound:tacrolimus|FD-Score:8.04|P-value:6.55E-7 ID:SGTC_1114|Compound:scopoletin|FD-Score:5.39|P-value:6.06E-4 ID:SGTC_1149|Compound:3909-7961|FD-Score:6.18|P-value:1.02E-4 ID:SGTC_1618|Compound:st003135|FD-Score:6.12|P-value:1.18E-4 ID:SGTC_1940|Compound:st075990|FD-Score:5.37|P-value:6.38E-4 ID:SGTC_1953|Compound:st076800|FD-Score:6.09|P-value:1.28E-4 ID:SGTC_1975|Compound:st070967|FD-Score:7.09|P-value:1.02E-5 ID:SGTC_1991|Compound:st073000|FD-Score:5.65|P-value:3.46E-4 ID:SGTC_2013|Compound:4024628|FD-Score:7.44|P-value:3.82E-6 ID:SGTC_2111|Compound:5486399|FD-Score:-5.69|P-value:2.51E-4 ID:SGTC_2168|Compound:5727979|FD-Score:6.30|P-value:7.62E-5 ID:SGTC_2242|Compound:6630449|FD-Score:6.48|P-value:4.96E-5 ID:SGTC_2277|Compound:7947813|FD-Score:5.76|P-value:2.70E-4 ID:SGTC_2330|Compound:7979373|FD-Score:8.73|P-value:7.40E-8 ID:SGTC_2332|Compound:7982454|FD-Score:6.64|P-value:3.27E-5 ID:SGTC_2413|Compound:inermin|FD-Score:7.70|P-value:1.81E-6 ID:SGTC_2431|Compound:5490079|FD-Score:5.17|P-value:9.64E-4 ID:SGTC_2440|Compound:5628896|FD-Score:10.10|P-value:5.16E-10 ID:SGTC_2443|Compound:5493264|FD-Score:7.55|P-value:2.78E-6 ID:SGTC_2445|Compound:5742597|FD-Score:5.49|P-value:4.88E-4 ID:SGTC_2460|Compound:5552655|FD-Score:5.34|P-value:6.85E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:5.29|P-value:7.61E-4 ID:SGTC_2556|Compound:citropten|FD-Score:5.63|P-value:3.62E-4 ID:SGTC_2600|Compound:tangeritin|FD-Score:8.37|P-value:2.31E-7 ID:SGTC_2686|Compound:alizarin|FD-Score:6.04|P-value:1.43E-4 ID:SGTC_2689|Compound:tacrolimus|FD-Score:9.68|P-value:2.69E-9 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:11.20|P-value:8.68E-12 ID:SGTC_2699|Compound:st077704|FD-Score:5.22|P-value:8.77E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:6.75|P-value:2.50E-5 ID:SGTC_2752|Compound:diclazuril|FD-Score:6.17|P-value:1.05E-4 ID:SGTC_2824|Compound:7998404|FD-Score:6.79|P-value:2.22E-5 ID:SGTC_2826|Compound:7992541|FD-Score:5.19|P-value:9.18E-4 ID:SGTC_2873|Compound:9040596|FD-Score:5.36|P-value:6.45E-4 ID:SGTC_2954|Compound:9074818|FD-Score:7.37|P-value:4.61E-6 ID:SGTC_3055|Compound:9094636|FD-Score:5.22|P-value:8.72E-4 ID:SGTC_3062|Compound:9077494|FD-Score:6.29|P-value:7.85E-5 ID:SGTC_3098|Compound:9119039|FD-Score:5.57|P-value:4.15E-4 ID:SGTC_3225|Compound:9130819|FD-Score:5.33|P-value:6.96E-4 ID:SGTC_3307|Compound:9126885|FD-Score:10.10|P-value:5.80E-10 ID:SGTC_3350|Compound:9150499|FD-Score:7.91|P-value:9.72E-7 ID:SGTC_66|Compound:0109-0187|FD-Score:5.64|P-value:3.55E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:6.72|P-value:2.66E-5 ID:SGTC_169|Compound:k015-0017|FD-Score:-5.30|P-value:5.95E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:7.62|P-value:2.24E-6 ID:SGTC_232|Compound:clozapine|FD-Score:9.35|P-value:8.97E-9 ID:SGTC_301|Compound:4119-0100|FD-Score:5.90|P-value:1.97E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:5.50|P-value:4.81E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:6.81|P-value:2.13E-5 ID:SGTC_389|Compound:tpck|FD-Score:5.35|P-value:6.70E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:6.07|P-value:1.34E-4 ID:SGTC_449|Compound:xestospongin c|FD-Score:7.17|P-value:8.01E-6 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-8.59|P-value:8.25E-8 ID:SGTC_459|Compound:nigericin|FD-Score:11.50|P-value:1.65E-12 ID:SGTC_488|Compound:nitrendipine|FD-Score:5.93|P-value:1.85E-4 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:6.23|P-value:9.09E-5 ID:SGTC_706|Compound:4262-0057|FD-Score:-5.26|P-value:6.45E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:7.56|P-value:2.73E-6 ID:SGTC_949|Compound:1165-0505|FD-Score:6.97|P-value:1.40E-5 ID:SGTC_1010|Compound:4112-3296|FD-Score:6.26|P-value:8.46E-5 ID:SGTC_1026|Compound:k007-1105|FD-Score:10.30|P-value:2.83E-10 ID:SGTC_1060|Compound:3448-7350|FD-Score:5.78|P-value:2.61E-4 ID:SGTC_1069|Compound:diphenylcyclopropenone|FD-Score:6.93|P-value:1.55E-5 ID:SGTC_1078|Compound:idebenone|FD-Score:6.62|P-value:3.46E-5 ID:SGTC_1104|Compound:tacrolimus|FD-Score:8.04|P-value:6.55E-7 ID:SGTC_1114|Compound:scopoletin|FD-Score:5.39|P-value:6.06E-4 ID:SGTC_1149|Compound:3909-7961|FD-Score:6.18|P-value:1.02E-4 ID:SGTC_1618|Compound:st003135|FD-Score:6.12|P-value:1.18E-4 ID:SGTC_1940|Compound:st075990|FD-Score:5.37|P-value:6.38E-4 ID:SGTC_1953|Compound:st076800|FD-Score:6.09|P-value:1.28E-4 ID:SGTC_1975|Compound:st070967|FD-Score:7.09|P-value:1.02E-5 ID:SGTC_1991|Compound:st073000|FD-Score:5.65|P-value:3.46E-4 ID:SGTC_2013|Compound:4024628|FD-Score:7.44|P-value:3.82E-6 ID:SGTC_2111|Compound:5486399|FD-Score:-5.69|P-value:2.51E-4 ID:SGTC_2168|Compound:5727979|FD-Score:6.30|P-value:7.62E-5 ID:SGTC_2242|Compound:6630449|FD-Score:6.48|P-value:4.96E-5 ID:SGTC_2277|Compound:7947813|FD-Score:5.76|P-value:2.70E-4 ID:SGTC_2330|Compound:7979373|FD-Score:8.73|P-value:7.40E-8 ID:SGTC_2332|Compound:7982454|FD-Score:6.64|P-value:3.27E-5 ID:SGTC_2413|Compound:inermin|FD-Score:7.70|P-value:1.81E-6 ID:SGTC_2431|Compound:5490079|FD-Score:5.17|P-value:9.64E-4 ID:SGTC_2440|Compound:5628896|FD-Score:10.10|P-value:5.16E-10 ID:SGTC_2443|Compound:5493264|FD-Score:7.55|P-value:2.78E-6 ID:SGTC_2445|Compound:5742597|FD-Score:5.49|P-value:4.88E-4 ID:SGTC_2460|Compound:5552655|FD-Score:5.34|P-value:6.85E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:5.29|P-value:7.61E-4 ID:SGTC_2556|Compound:citropten|FD-Score:5.63|P-value:3.62E-4 ID:SGTC_2600|Compound:tangeritin|FD-Score:8.37|P-value:2.31E-7 ID:SGTC_2686|Compound:alizarin|FD-Score:6.04|P-value:1.43E-4 ID:SGTC_2689|Compound:tacrolimus|FD-Score:9.68|P-value:2.69E-9 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:11.20|P-value:8.68E-12 ID:SGTC_2699|Compound:st077704|FD-Score:5.22|P-value:8.77E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:6.75|P-value:2.50E-5 ID:SGTC_2752|Compound:diclazuril|FD-Score:6.17|P-value:1.05E-4 ID:SGTC_2824|Compound:7998404|FD-Score:6.79|P-value:2.22E-5 ID:SGTC_2826|Compound:7992541|FD-Score:5.19|P-value:9.18E-4 ID:SGTC_2873|Compound:9040596|FD-Score:5.36|P-value:6.45E-4 ID:SGTC_2954|Compound:9074818|FD-Score:7.37|P-value:4.61E-6 ID:SGTC_3055|Compound:9094636|FD-Score:5.22|P-value:8.72E-4 ID:SGTC_3062|Compound:9077494|FD-Score:6.29|P-value:7.85E-5 ID:SGTC_3098|Compound:9119039|FD-Score:5.57|P-value:4.15E-4 ID:SGTC_3225|Compound:9130819|FD-Score:5.33|P-value:6.96E-4 ID:SGTC_3307|Compound:9126885|FD-Score:10.10|P-value:5.80E-10 ID:SGTC_3350|Compound:9150499|FD-Score:7.91|P-value:9.72E-7

Top fitness defect scores for YOL018C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_459 nigericin 11.50 15.3 µM 1.65E-12 4 3.72E-31
2 SGTC_2697 mebhydrolin 11.20 72.4 µM 8.68E-12 NEO1 13 3.32E-29
3 SGTC_1026 k007-1105 10.30 30.6 µM 2.83E-10 ERAD & cell cycle 11 4.06E-25
4 SGTC_2440 5628896 10.10 157.8 µM 5.16E-10 1 2.05E-24
5 SGTC_3307 9126885 10.10 69.3 µM 5.80E-10 1 2.81E-24
6 SGTC_2689 tacrolimus 9.68 100.0 µM 2.69E-9 4 1.76E-22
7 SGTC_232 clozapine 9.35 119.0 µM 8.97E-9 NEO1 16 4.50E-21
8 SGTC_2330 7979373 8.73 200.0 µM 7.40E-8 60S ribosome export 4 1.31E-18
9 SGTC_2600 tangeritin 8.37 88.0 µM 2.31E-7 4 2.78E-17
10 SGTC_1104 tacrolimus 8.04 29.8 µM 6.55E-7 8 4.53E-16
11 SGTC_3350 9150499 7.91 3.6 µM 9.72E-7 cell wall 7 1.31E-15
12 SGTC_2413 inermin 7.70 26.1 µM 1.81E-6 11 6.87E-15
13 SGTC_221 trifluoperazine 7.62 10.3 µM 2.24E-6 NEO1-PIK1 89 1.22E-14
14 SGTC_946 3434-1334 7.56 87.4 µM 2.73E-6 2 2.06E-14
15 SGTC_2443 5493264 7.55 126.7 µM 2.78E-6 1 2.18E-14
16 SGTC_2013 4024628 7.44 200.0 µM 3.82E-6 9 5.08E-14
17 SGTC_2954 9074818 7.37 52.7 µM 4.61E-6 5 8.35E-14
18 SGTC_449 xestospongin c 7.17 11.2 µM 8.01E-6 NEO1-PIK1 11 3.65E-13
19 SGTC_1975 st070967 7.09 30.8 µM 1.02E-5 7 6.89E-13
20 SGTC_949 1165-0505 6.97 146.0 µM 1.40E-5 4 1.63E-12

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.439 7.12E-157 YKR019C IRS4 hom EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
0.294 1.73E-67 YML071C COG8 hom Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
0.292 1.05E-66 YGL005C COG7 hom Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
0.283 2.47E-62 YJL004C SYS1 hom Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
0.279 8.04E-61 YNL041C COG6 hom Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
0.256 4.30E-51 YGL095C VPS45 hom Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment
0.238 3.33E-44 YKR020W VPS51 hom Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p
0.230 2.54E-41 YKR001C VPS1 hom Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis
0.229 5.14E-41 YDR484W VPS52 hom Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin
0.228 1.15E-40 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.223 7.50E-39 YHR012W VPS29 hom Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
0.216 2.05E-36 YLR262C YPT6 hom Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6
0.204 1.04E-32 YJL053W PEP8 hom Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
0.203 1.85E-32 YDR108W TRS85 hom Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role
0.199 3.66E-31 YDR137W RGP1 hom Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p