YOL021C / DIS3

Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOL021C / DIS3

Click on Significant Values for Screen Details ID:SGTC_25|Compound:0118-0223|FD-Score:4.23|P-value:3.96E-4 ID:SGTC_109|Compound:0132-0036|FD-Score:-4.39|P-value:2.35E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:10.00|P-value:8.37E-16 ID:SGTC_195|Compound:4234-0266|FD-Score:4.30|P-value:3.29E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:5.71|P-value:2.97E-6 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:19.10|P-value:4.33E-52 ID:SGTC_258|Compound:haloperidol|FD-Score:4.46|P-value:2.03E-4 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:11.50|P-value:4.76E-20 ID:SGTC_274|Compound:sirtinol|FD-Score:4.18|P-value:4.58E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:7.29|P-value:3.54E-9 ID:SGTC_610|Compound:4215-0184|FD-Score:11.20|P-value:2.80E-19 ID:SGTC_617|Compound:3770-0098|FD-Score:13.40|P-value:9.33E-27 ID:SGTC_736|Compound:0958-0254|FD-Score:7.14|P-value:7.37E-9 ID:SGTC_743|Compound:1275-0778|FD-Score:-4.41|P-value:2.19E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:4.82|P-value:6.60E-5 ID:SGTC_893|Compound:0958-0369|FD-Score:5.96|P-value:1.11E-6 ID:SGTC_902|Compound:1120-0002|FD-Score:-4.24|P-value:3.63E-4 ID:SGTC_905|Compound:2-aminofluorene|FD-Score:-4.85|P-value:5.64E-5 ID:SGTC_913|Compound:3487-1245|FD-Score:4.55|P-value:1.56E-4 ID:SGTC_1074|Compound:carmofur|FD-Score:16.00|P-value:2.02E-37 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:12.30|P-value:1.07E-22 ID:SGTC_1125|Compound:tnp00248|FD-Score:4.09|P-value:5.96E-4 ID:SGTC_1260|Compound:0700-1617|FD-Score:4.25|P-value:3.75E-4 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:4.37|P-value:2.67E-4 ID:SGTC_1611|Compound:st001856|FD-Score:4.19|P-value:4.50E-4 ID:SGTC_1698|Compound:5-fluorocytosine|FD-Score:9.51|P-value:2.20E-14 ID:SGTC_1786|Compound:berberine|FD-Score:-4.10|P-value:5.37E-4 ID:SGTC_1827|Compound:berberine|FD-Score:-3.99|P-value:7.43E-4 ID:SGTC_1855|Compound:st056195|FD-Score:-4.75|P-value:7.62E-5 ID:SGTC_2036|Compound:5160441|FD-Score:-4.49|P-value:1.73E-4 ID:SGTC_2038|Compound:4100038|FD-Score:-4.72|P-value:8.33E-5 ID:SGTC_2048|Compound:5246248|FD-Score:-4.64|P-value:1.08E-4 ID:SGTC_2444|Compound:5485484|FD-Score:-3.92|P-value:8.96E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:-4.54|P-value:1.49E-4 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:-4.76|P-value:7.50E-5 ID:SGTC_2619|Compound:obliquin|FD-Score:-3.88|P-value:9.78E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:-5.36|P-value:9.77E-6 ID:SGTC_2625|Compound:euphol|FD-Score:-4.22|P-value:3.89E-4 ID:SGTC_2705|Compound:st078069|FD-Score:-4.15|P-value:4.69E-4 ID:SGTC_2714|Compound:st079313|FD-Score:4.64|P-value:1.18E-4 ID:SGTC_2716|Compound:st080858|FD-Score:-4.43|P-value:2.07E-4 ID:SGTC_2732|Compound:terfenadine|FD-Score:4.07|P-value:6.34E-4 ID:SGTC_2739|Compound:tacrine|FD-Score:-5.82|P-value:1.81E-6 ID:SGTC_2743|Compound:auranofin|FD-Score:-4.14|P-value:4.77E-4 ID:SGTC_2996|Compound:9066192|FD-Score:4.13|P-value:5.26E-4 ID:SGTC_3032|Compound:9089427|FD-Score:-4.24|P-value:3.67E-4 ID:SGTC_3178|Compound:9107506|FD-Score:4.16|P-value:4.92E-4 ID:SGTC_3189|Compound:9110682|FD-Score:-4.21|P-value:3.96E-4 ID:SGTC_3221|Compound:9128209|FD-Score:5.90|P-value:1.44E-6 ID:SGTC_3223|Compound:9129970|FD-Score:4.09|P-value:5.88E-4 ID:SGTC_3249|Compound:9135183|FD-Score:-3.99|P-value:7.32E-4 ID:SGTC_3287|Compound:9117173|FD-Score:-3.89|P-value:9.59E-4 ID:SGTC_3337|Compound:9144342|FD-Score:8.18|P-value:4.12E-11 ID:SGTC_25|Compound:0118-0223|FD-Score:4.23|P-value:3.96E-4 ID:SGTC_109|Compound:0132-0036|FD-Score:-4.39|P-value:2.35E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:10.00|P-value:8.37E-16 ID:SGTC_195|Compound:4234-0266|FD-Score:4.30|P-value:3.29E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:5.71|P-value:2.97E-6 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:19.10|P-value:4.33E-52 ID:SGTC_258|Compound:haloperidol|FD-Score:4.46|P-value:2.03E-4 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:11.50|P-value:4.76E-20 ID:SGTC_274|Compound:sirtinol|FD-Score:4.18|P-value:4.58E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:7.29|P-value:3.54E-9 ID:SGTC_610|Compound:4215-0184|FD-Score:11.20|P-value:2.80E-19 ID:SGTC_617|Compound:3770-0098|FD-Score:13.40|P-value:9.33E-27 ID:SGTC_736|Compound:0958-0254|FD-Score:7.14|P-value:7.37E-9 ID:SGTC_743|Compound:1275-0778|FD-Score:-4.41|P-value:2.19E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:4.82|P-value:6.60E-5 ID:SGTC_893|Compound:0958-0369|FD-Score:5.96|P-value:1.11E-6 ID:SGTC_902|Compound:1120-0002|FD-Score:-4.24|P-value:3.63E-4 ID:SGTC_905|Compound:2-aminofluorene|FD-Score:-4.85|P-value:5.64E-5 ID:SGTC_913|Compound:3487-1245|FD-Score:4.55|P-value:1.56E-4 ID:SGTC_1074|Compound:carmofur|FD-Score:16.00|P-value:2.02E-37 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:12.30|P-value:1.07E-22 ID:SGTC_1125|Compound:tnp00248|FD-Score:4.09|P-value:5.96E-4 ID:SGTC_1260|Compound:0700-1617|FD-Score:4.25|P-value:3.75E-4 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:4.37|P-value:2.67E-4 ID:SGTC_1611|Compound:st001856|FD-Score:4.19|P-value:4.50E-4 ID:SGTC_1698|Compound:5-fluorocytosine|FD-Score:9.51|P-value:2.20E-14 ID:SGTC_1786|Compound:berberine|FD-Score:-4.10|P-value:5.37E-4 ID:SGTC_1827|Compound:berberine|FD-Score:-3.99|P-value:7.43E-4 ID:SGTC_1855|Compound:st056195|FD-Score:-4.75|P-value:7.62E-5 ID:SGTC_2036|Compound:5160441|FD-Score:-4.49|P-value:1.73E-4 ID:SGTC_2038|Compound:4100038|FD-Score:-4.72|P-value:8.33E-5 ID:SGTC_2048|Compound:5246248|FD-Score:-4.64|P-value:1.08E-4 ID:SGTC_2444|Compound:5485484|FD-Score:-3.92|P-value:8.96E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:-4.54|P-value:1.49E-4 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:-4.76|P-value:7.50E-5 ID:SGTC_2619|Compound:obliquin|FD-Score:-3.88|P-value:9.78E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:-5.36|P-value:9.77E-6 ID:SGTC_2625|Compound:euphol|FD-Score:-4.22|P-value:3.89E-4 ID:SGTC_2705|Compound:st078069|FD-Score:-4.15|P-value:4.69E-4 ID:SGTC_2714|Compound:st079313|FD-Score:4.64|P-value:1.18E-4 ID:SGTC_2716|Compound:st080858|FD-Score:-4.43|P-value:2.07E-4 ID:SGTC_2732|Compound:terfenadine|FD-Score:4.07|P-value:6.34E-4 ID:SGTC_2739|Compound:tacrine|FD-Score:-5.82|P-value:1.81E-6 ID:SGTC_2743|Compound:auranofin|FD-Score:-4.14|P-value:4.77E-4 ID:SGTC_2996|Compound:9066192|FD-Score:4.13|P-value:5.26E-4 ID:SGTC_3032|Compound:9089427|FD-Score:-4.24|P-value:3.67E-4 ID:SGTC_3178|Compound:9107506|FD-Score:4.16|P-value:4.92E-4 ID:SGTC_3189|Compound:9110682|FD-Score:-4.21|P-value:3.96E-4 ID:SGTC_3221|Compound:9128209|FD-Score:5.90|P-value:1.44E-6 ID:SGTC_3223|Compound:9129970|FD-Score:4.09|P-value:5.88E-4 ID:SGTC_3249|Compound:9135183|FD-Score:-3.99|P-value:7.32E-4 ID:SGTC_3287|Compound:9117173|FD-Score:-3.89|P-value:9.59E-4 ID:SGTC_3337|Compound:9144342|FD-Score:8.18|P-value:4.12E-11

Top fitness defect scores for YOL021C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_242 5hr heat shock (37°C) + 5-fluorouracil 19.10 4.6 µM 4.33E-52 heat shock/prefoldin 1 2.83E-81 5.16
2 SGTC_1074 carmofur 16.00 3.8 µM 2.02E-37 exosome 2 4.32E-58 2.88
3 SGTC_617 3770-0098 13.40 35.0 µM 9.33E-27 exosome 3 3.01E-41 0.95
4 SGTC_1077 5-fluorocytosine 12.30 377.0 nM 1.07E-22 exosome 2 7.64E-35 1.07
5 SGTC_261 5-fluorouridine 11.50 15.4 µM 4.76E-20 exosome 2 1.16E-30 4.60
6 SGTC_610 4215-0184 11.20 155.0 µM 2.80E-19 exosome 3 1.89E-29 2.19
7 SGTC_118 0083-0097 10.00 135.3 µM 8.37E-16 translation 1 5.70E-24 3.26
8 SGTC_1698 5-fluorocytosine 9.51 1.1 µM 2.20E-14 exosome 3 9.83E-22 0.92
9 SGTC_3337 9144342 8.18 33.5 µM 4.12E-11 2 1.38E-16 1.40
10 SGTC_423 5-fluorouracil 7.29 457.0 µM 3.54E-9 exosome 9 1.51E-13 0.15
11 SGTC_736 0958-0254 7.14 92.6 µM 7.37E-9 Golgi 1 4.77E-13 2.42
12 SGTC_893 0958-0369 5.96 48.9 µM 1.11E-6 1 1.24E-9 1.43
13 SGTC_3221 9128209 5.90 49.5 µM 1.44E-6 2 1.83E-9 1.16
14 SGTC_236 1509-0011 5.71 5.3 µM 2.97E-6 10 5.72E-9 0.11
15 SGTC_800 3448-7362 4.82 155.0 µM 6.60E-5 translation 35 7.17E-7 0.07
16 SGTC_2714 st079313 4.64 56.9 µM 1.18E-4 4 1.76E-6 0.77
17 SGTC_913 3487-1245 4.55 167.0 µM 1.56E-4 9 2.73E-6 0.22
18 SGTC_258 haloperidol 4.46 50.8 µM 2.03E-4 fatty acid desaturase (OLE1) 17 4.10E-6 0.07
19 SGTC_1566 6,7-dimethoxy-4-ethylcoumarin 4.37 85.4 µM 2.67E-4 2 6.26E-6 0.56
20 SGTC_195 4234-0266 4.30 16.6 µM 3.29E-4 10 8.68E-6 0.27

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.589 7.87E-313 YGR195W SKI6 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
0.530 4.50E-242 YDL111C RRP42 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
0.500 4.19E-212 YGR158C MTR3 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
0.445 9.76E-163 YDR280W RRP45 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
0.378 2.10E-114 YGR095C RRP46 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
0.340 2.25E-91 YKL172W EBP2 het Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
0.331 9.68E-87 YPL043W NOP4 het Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs)
0.323 4.92E-82 YPR142C_d YPR142C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing
0.313 5.74E-77 YPL044C_d YPL044C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W
0.292 1.08E-66 YNL061W NOP2 het Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles
0.277 3.34E-60 YPR143W RRP15 het Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
0.268 2.22E-56 YNL232W CSL4 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1)
0.268 5.27E-56 YNL002C RLP7 het Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs
0.203 1.75E-32 YHR069C RRP4 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
0.180 6.99E-26 YOL077C BRX1 het Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif